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The map label for this gene is prfC [H]

Identifier: 162147981

GI number: 162147981

Start: 2248091

End: 2249674

Strand: Direct

Name: prfC [H]

Synonym: GDI_2197

Alternate gene names: 162147981

Gene position: 2248091-2249674 (Clockwise)

Preceding gene: 162147980

Following gene: 162147983

Centisome position: 57.0

GC content: 68.18

Gene sequence:

>1584_bases
ATGGATGCGCCCGTGAACGCCGAGCCCGCAACCGCCCCCACATCCGGCCTAGCCGCCACCATCGCCCGGGAAATCGCCCG
GCGGCGCACCTTCGCCATCATCTCGCACCCAGACGCGGGCAAGACCACGCTGACCGAGCGCATCCTGCGCGCGGGCGGCG
CGATCCAGATGGCGGGCAATGTCCGGGCCAAGGGCGAACGCCGCCGCACCCGGTCGGACTGGATGGGGATCGAGCGCGAT
CGCGGCATTTCGGTCGTGACCTCGGTCATGACGTTCGAATATGGCGGCTGCATCTTCAACCTGCTGGACACGCCGGGCCA
CGAGGATTTTTCGGAAGATACCTATCGCACGCTGACGGCCGTCGATGCGGCGGTGATGGTGATCGACGCCGCCAAAGGGA
TCGAGGACCGGACCCGCAAGCTGTTCGAGATCTGCCGCCTGCGCGACATTCCGATCGTCACCTTCATCAACAAGATGGAC
CGCGAGGCGCAGGACCCGTTCACGCTGCTGGACGAAATCTCGTCCGCGCTGGCGCTGGATACCGCGCCGGCGACGTGGCC
GGTCGGCCGTGCGGCGCAGTTCGTCGGCACGTATGACCTGCGCGCGCGCAGCCTGCATGTGTCCACGCCGCTGGAGCAGT
CCGACCCGCGCATGGTGCAACTGGCCGAGGACCTGGAACTGGCCGAGGCCGCCCTGCCGGTCTTCGACCGCGACAGCTTC
AATGCCGGGCACCTGACCCCGGTCTTCTTCGGCAGCGCGATGAAGGAGATCGGCGTGACCGACCTGCTGGACGCGCTGGT
GGCGTTCGGCCCGCCGCCGCGCGATCAGGCGACCGAAAGCCGGACGGTGCGCGCGGACGAGACCGGACTGACCGCGCTGG
TCTTCAAGATCCAGGCCAACATGGACCCGAACCATCGCGACCGCATGGCCTTCGCGCGGATCTGCTCGGGCCGGCTGGAA
CGCGGGATGCGGCTGAAGCATGTGCGCATCGGCAAGCAGTTCGCGCTGCATACGCCGCAATTCTTCTTCGCCCGCGACCG
GCAGCTGGCCGAGGAAGCCTTCGCCGGCGACGTGGTGGGCATTCCCAACCACGGCACCCTGCGCATCGGCGACACGCTGA
CCGAGGGCGAGGACCTGCGCTTTACCGGCGTGCCGCACTTCGCCCCGGAAATCCTGCGCCGCGTCCGGCTGGACGACGCG
ATGAAGGCCAAGAAGCTGCGCCAGGCCCTGACGGAACTGGCCGAGGAAGGGGTGGTTCAGCTCTTCCGCCCGCAGGACGG
CGCGCCGCCCATCGTCGGCGTCGTCGGCACGCTGCAGCTCGACGTGCTGCAGGCGCGGCTGTCCGGGGAATACGGGGTGG
CGATCGGCTTCGAATCCACCCCCTACAACCTGGCCCGCTGGGTGACGGGCGACCGCGCGAAGCTGGAGACGTTCGCGATG
GCCAACCGCTCGGCCATGGCGGACGACCTGGACGGCGATCCGGTCTTCCTGGCCGGATCGGCCTTCATGATGCGTCGCAC
GGCCGAGATGAACCTCGACCTGTCATTCCACGACATCAAGCAGATCGGCATCGAGACACGCTGA

Upstream 100 bases:

>100_bases
CGATCGCGGCGCCGGGCGCGGGGGACATCCCCCTCGCCCGGCGTCCGTGTTTTGGGACCCCGCCGGACCGCATCGCGGCC
CGGCCGACATCGCAGCACGA

Downstream 100 bases:

>100_bases
CCGCCGCGGGCCCGCGATCGCCCAGCGTCCGCGCGGCCCGCACCCCCAGCATCAGCGCCACCGCCACCAGCGCGCACGCC
ACGCCGCCCCACAGGCCGAC

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 527; Mature: 527

Protein sequence:

>527_residues
MDAPVNAEPATAPTSGLAATIAREIARRRTFAIISHPDAGKTTLTERILRAGGAIQMAGNVRAKGERRRTRSDWMGIERD
RGISVVTSVMTFEYGGCIFNLLDTPGHEDFSEDTYRTLTAVDAAVMVIDAAKGIEDRTRKLFEICRLRDIPIVTFINKMD
REAQDPFTLLDEISSALALDTAPATWPVGRAAQFVGTYDLRARSLHVSTPLEQSDPRMVQLAEDLELAEAALPVFDRDSF
NAGHLTPVFFGSAMKEIGVTDLLDALVAFGPPPRDQATESRTVRADETGLTALVFKIQANMDPNHRDRMAFARICSGRLE
RGMRLKHVRIGKQFALHTPQFFFARDRQLAEEAFAGDVVGIPNHGTLRIGDTLTEGEDLRFTGVPHFAPEILRRVRLDDA
MKAKKLRQALTELAEEGVVQLFRPQDGAPPIVGVVGTLQLDVLQARLSGEYGVAIGFESTPYNLARWVTGDRAKLETFAM
ANRSAMADDLDGDPVFLAGSAFMMRRTAEMNLDLSFHDIKQIGIETR

Sequences:

>Translated_527_residues
MDAPVNAEPATAPTSGLAATIAREIARRRTFAIISHPDAGKTTLTERILRAGGAIQMAGNVRAKGERRRTRSDWMGIERD
RGISVVTSVMTFEYGGCIFNLLDTPGHEDFSEDTYRTLTAVDAAVMVIDAAKGIEDRTRKLFEICRLRDIPIVTFINKMD
REAQDPFTLLDEISSALALDTAPATWPVGRAAQFVGTYDLRARSLHVSTPLEQSDPRMVQLAEDLELAEAALPVFDRDSF
NAGHLTPVFFGSAMKEIGVTDLLDALVAFGPPPRDQATESRTVRADETGLTALVFKIQANMDPNHRDRMAFARICSGRLE
RGMRLKHVRIGKQFALHTPQFFFARDRQLAEEAFAGDVVGIPNHGTLRIGDTLTEGEDLRFTGVPHFAPEILRRVRLDDA
MKAKKLRQALTELAEEGVVQLFRPQDGAPPIVGVVGTLQLDVLQARLSGEYGVAIGFESTPYNLARWVTGDRAKLETFAM
ANRSAMADDLDGDPVFLAGSAFMMRRTAEMNLDLSFHDIKQIGIETR
>Mature_527_residues
MDAPVNAEPATAPTSGLAATIAREIARRRTFAIISHPDAGKTTLTERILRAGGAIQMAGNVRAKGERRRTRSDWMGIERD
RGISVVTSVMTFEYGGCIFNLLDTPGHEDFSEDTYRTLTAVDAAVMVIDAAKGIEDRTRKLFEICRLRDIPIVTFINKMD
REAQDPFTLLDEISSALALDTAPATWPVGRAAQFVGTYDLRARSLHVSTPLEQSDPRMVQLAEDLELAEAALPVFDRDSF
NAGHLTPVFFGSAMKEIGVTDLLDALVAFGPPPRDQATESRTVRADETGLTALVFKIQANMDPNHRDRMAFARICSGRLE
RGMRLKHVRIGKQFALHTPQFFFARDRQLAEEAFAGDVVGIPNHGTLRIGDTLTEGEDLRFTGVPHFAPEILRRVRLDDA
MKAKKLRQALTELAEEGVVQLFRPQDGAPPIVGVVGTLQLDVLQARLSGEYGVAIGFESTPYNLARWVTGDRAKLETFAM
ANRSAMADDLDGDPVFLAGSAFMMRRTAEMNLDLSFHDIKQIGIETR

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI19923640, Length=509, Percent_Identity=26.9155206286837, Blast_Score=133, Evalue=5e-31,
Organism=Homo sapiens, GI18390331, Length=477, Percent_Identity=26.2054507337526, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI25306283, Length=355, Percent_Identity=29.5774647887324, Blast_Score=129, Evalue=6e-30,
Organism=Homo sapiens, GI25306287, Length=305, Percent_Identity=30.4918032786885, Blast_Score=119, Evalue=1e-26,
Organism=Homo sapiens, GI157426893, Length=150, Percent_Identity=33.3333333333333, Blast_Score=89, Evalue=7e-18,
Organism=Homo sapiens, GI94966754, Length=147, Percent_Identity=29.9319727891156, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI4503483, Length=154, Percent_Identity=31.8181818181818, Blast_Score=68, Evalue=3e-11,
Organism=Escherichia coli, GI1790835, Length=525, Percent_Identity=49.7142857142857, Blast_Score=514, Evalue=1e-147,
Organism=Escherichia coli, GI1789738, Length=487, Percent_Identity=26.694045174538, Blast_Score=138, Evalue=7e-34,
Organism=Escherichia coli, GI1788922, Length=180, Percent_Identity=33.3333333333333, Blast_Score=96, Evalue=7e-21,
Organism=Escherichia coli, GI48994988, Length=149, Percent_Identity=30.2013422818792, Blast_Score=82, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17533571, Length=501, Percent_Identity=26.3473053892216, Blast_Score=149, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI17556745, Length=463, Percent_Identity=25.2699784017279, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17557151, Length=137, Percent_Identity=33.5766423357664, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17506493, Length=161, Percent_Identity=27.9503105590062, Blast_Score=68, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71988819, Length=135, Percent_Identity=30.3703703703704, Blast_Score=68, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71988811, Length=135, Percent_Identity=30.3703703703704, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323098, Length=476, Percent_Identity=26.2605042016807, Blast_Score=148, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6322359, Length=416, Percent_Identity=28.125, Blast_Score=117, Evalue=5e-27,
Organism=Saccharomyces cerevisiae, GI6323320, Length=176, Percent_Identity=27.8409090909091, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6324707, Length=160, Percent_Identity=28.125, Blast_Score=66, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6320593, Length=160, Percent_Identity=28.125, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24582462, Length=486, Percent_Identity=26.5432098765432, Blast_Score=139, Evalue=4e-33,
Organism=Drosophila melanogaster, GI221458488, Length=391, Percent_Identity=29.6675191815857, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI78706572, Length=160, Percent_Identity=33.125, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24585709, Length=155, Percent_Identity=29.6774193548387, Blast_Score=67, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24585711, Length=155, Percent_Identity=29.6774193548387, Blast_Score=67, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24585713, Length=155, Percent_Identity=29.6774193548387, Blast_Score=67, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 58098; Mature: 58098

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAPVNAEPATAPTSGLAATIAREIARRRTFAIISHPDAGKTTLTERILRAGGAIQMAGN
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCEEEEEECCCCCHHHHHHHHHHCCCCEEECCC
VRAKGERRRTRSDWMGIERDRGISVVTSVMTFEYGGCIFNLLDTPGHEDFSEDTYRTLTA
CCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHH
VDAAVMVIDAAKGIEDRTRKLFEICRLRDIPIVTFINKMDREAQDPFTLLDEISSALALD
HHHHHHEEHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHEEC
TAPATWPVGRAAQFVGTYDLRARSLHVSTPLEQSDPRMVQLAEDLELAEAALPVFDRDSF
CCCCCCCCCHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
NAGHLTPVFFGSAMKEIGVTDLLDALVAFGPPPRDQATESRTVRADETGLTALVFKIQAN
CCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCEEEEEEEECC
MDPNHRDRMAFARICSGRLERGMRLKHVRIGKQFALHTPQFFFARDRQLAEEAFAGDVVG
CCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCHHCCCCCHHHHHHHHHHHHHHHCCCEEE
IPNHGTLRIGDTLTEGEDLRFTGVPHFAPEILRRVRLDDAMKAKKLRQALTELAEEGVVQ
CCCCCEEEECCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHE
LFRPQDGAPPIVGVVGTLQLDVLQARLSGEYGVAIGFESTPYNLARWVTGDRAKLETFAM
EECCCCCCCCCEEHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHCCCCHHHHHHHHH
ANRSAMADDLDGDPVFLAGSAFMMRRTAEMNLDLSFHDIKQIGIETR
HCCHHCCCCCCCCEEEEECCHHHHHHHHHCCCCCCHHHHHHHCCCCC
>Mature Secondary Structure
MDAPVNAEPATAPTSGLAATIAREIARRRTFAIISHPDAGKTTLTERILRAGGAIQMAGN
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCEEEEEECCCCCHHHHHHHHHHCCCCEEECCC
VRAKGERRRTRSDWMGIERDRGISVVTSVMTFEYGGCIFNLLDTPGHEDFSEDTYRTLTA
CCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHH
VDAAVMVIDAAKGIEDRTRKLFEICRLRDIPIVTFINKMDREAQDPFTLLDEISSALALD
HHHHHHEEHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHEEC
TAPATWPVGRAAQFVGTYDLRARSLHVSTPLEQSDPRMVQLAEDLELAEAALPVFDRDSF
CCCCCCCCCHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
NAGHLTPVFFGSAMKEIGVTDLLDALVAFGPPPRDQATESRTVRADETGLTALVFKIQAN
CCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCEEEEEEEECC
MDPNHRDRMAFARICSGRLERGMRLKHVRIGKQFALHTPQFFFARDRQLAEEAFAGDVVG
CCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCHHCCCCCHHHHHHHHHHHHHHHCCCEEE
IPNHGTLRIGDTLTEGEDLRFTGVPHFAPEILRRVRLDDAMKAKKLRQALTELAEEGVVQ
CCCCCEEEECCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHE
LFRPQDGAPPIVGVVGTLQLDVLQARLSGEYGVAIGFESTPYNLARWVTGDRAKLETFAM
EECCCCCCCCCEEHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHCCCCHHHHHHHHH
ANRSAMADDLDGDPVFLAGSAFMMRRTAEMNLDLSFHDIKQIGIETR
HCCHHCCCCCCCCEEEEECCHHHHHHHHHCCCCCCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA