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The map label for this gene is 162147970

Identifier: 162147970

GI number: 162147970

Start: 2237522

End: 2238226

Strand: Reverse

Name: 162147970

Synonym: GDI_2186

Alternate gene names: NA

Gene position: 2238226-2237522 (Counterclockwise)

Preceding gene: 162147976

Following gene: 162147967

Centisome position: 56.75

GC content: 66.81

Gene sequence:

>705_bases
ATGGAATGGACGGCGGAACGGGACTGGCCCCGCCTGGCAAGCGGCCTGGCCGGGCGGCTGCGGCGGGATTGCGGCGCCGA
ATGGGACGGCTTCATCCATCATCCCTTCGTGCGCGGCCTGGCGGACGGCAGCCTGCCCGAGGCCGAGTTCAGGCGGTTCC
TGATCCAGGACTACCTGTACCTGATCCAGTATGCCCGGGCCTACGCCCTGGCGATCTACAAGGCCGACAGGCTGGAGGAC
ATGCGCTCGGCCTCCGCCATCGTGTCGGGCCTGCTGGATACCGAACTGGCGCTGCACGTGTCCTATTGCACCGGCTGGGG
CCTGTGCATCGAGGATTTGCAGAGCCAGCCGGAATCGCTGGAACTGCTGGCCTATACGCGCTTCATCCTGGACCGGGGGC
AGGCGGGCGACCTTCTGGACCTGATGGTGACGCTGGCGCCCTGCCTGATCGGCTATGGCGAAATCGGCGTCCGGCTGGTG
GCCGATCCGCACACCAGGCGGGACGGCAATCCCTACTGGCCATGGATCGCGCTTTACGGCGGTGAGAAATTCATCGGCCT
GGTCGACGCCGGTATCGACACGCTGGAGGCATTGAGCCTGCGCTACGGCGCCGATGCGCGTTATCCGATGCTGCTGTCGG
AATTCCGGACCGCCGTGAAGCTGGAGGCCGCGTTCTGGCACGCTGGCCGTTCGGGCGCGCTCTGA

Upstream 100 bases:

>100_bases
TCGGGGCGAACCTGCTGCAATAAGGGAGCGTATCCTGCCGGGCGGAGTTCCTGCGGGAATCGTGCCGACCCCCGAACGGG
ACAGAAAAGGAGAACCAGCC

Downstream 100 bases:

>100_bases
AATTCAGGATCTGACGGCGTCGGCGCTGTTGCCTGCCGGTGGCGGAACACCACGCGGTGGCTGAGCGTGAAGTTGGCGAA
CAGCCCGGTCAGCGATCCGG

Product: thiaminase-2 protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MEWTAERDWPRLASGLAGRLRRDCGAEWDGFIHHPFVRGLADGSLPEAEFRRFLIQDYLYLIQYARAYALAIYKADRLED
MRSASAIVSGLLDTELALHVSYCTGWGLCIEDLQSQPESLELLAYTRFILDRGQAGDLLDLMVTLAPCLIGYGEIGVRLV
ADPHTRRDGNPYWPWIALYGGEKFIGLVDAGIDTLEALSLRYGADARYPMLLSEFRTAVKLEAAFWHAGRSGAL

Sequences:

>Translated_234_residues
MEWTAERDWPRLASGLAGRLRRDCGAEWDGFIHHPFVRGLADGSLPEAEFRRFLIQDYLYLIQYARAYALAIYKADRLED
MRSASAIVSGLLDTELALHVSYCTGWGLCIEDLQSQPESLELLAYTRFILDRGQAGDLLDLMVTLAPCLIGYGEIGVRLV
ADPHTRRDGNPYWPWIALYGGEKFIGLVDAGIDTLEALSLRYGADARYPMLLSEFRTAVKLEAAFWHAGRSGAL
>Mature_234_residues
MEWTAERDWPRLASGLAGRLRRDCGAEWDGFIHHPFVRGLADGSLPEAEFRRFLIQDYLYLIQYARAYALAIYKADRLED
MRSASAIVSGLLDTELALHVSYCTGWGLCIEDLQSQPESLELLAYTRFILDRGQAGDLLDLMVTLAPCLIGYGEIGVRLV
ADPHTRRDGNPYWPWIALYGGEKFIGLVDAGIDTLEALSLRYGADARYPMLLSEFRTAVKLEAAFWHAGRSGAL

Specific function: Unknown

COG id: COG0819

COG function: function code K; Putative transcription activator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiaminase-2 family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6324517, Length=204, Percent_Identity=32.3529411764706, Blast_Score=99, Evalue=8e-22,
Organism=Saccharomyces cerevisiae, GI6324997, Length=206, Percent_Identity=31.0679611650485, Blast_Score=90, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6325378, Length=204, Percent_Identity=30.3921568627451, Blast_Score=83, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016084
- InterPro:   IPR004305 [H]

Pfam domain/function: PF03070 TENA_THI-4 [H]

EC number: NA

Molecular weight: Translated: 26234; Mature: 26234

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00334 MYB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEWTAERDWPRLASGLAGRLRRDCGAEWDGFIHHPFVRGLADGSLPEAEFRRFLIQDYLY
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
LIQYARAYALAIYKADRLEDMRSASAIVSGLLDTELALHVSYCTGWGLCIEDLQSQPESL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHCCHHHHHHHHHCCCHHH
ELLAYTRFILDRGQAGDLLDLMVTLAPCLIGYGEIGVRLVADPHTRRDGNPYWPWIALYG
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCEEEEEC
GEKFIGLVDAGIDTLEALSLRYGADARYPMLLSEFRTAVKLEAAFWHAGRSGAL
CCEEEEHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MEWTAERDWPRLASGLAGRLRRDCGAEWDGFIHHPFVRGLADGSLPEAEFRRFLIQDYLY
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
LIQYARAYALAIYKADRLEDMRSASAIVSGLLDTELALHVSYCTGWGLCIEDLQSQPESL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHCCHHHHHHHHHCCCHHH
ELLAYTRFILDRGQAGDLLDLMVTLAPCLIGYGEIGVRLVADPHTRRDGNPYWPWIALYG
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCEEEEEC
GEKFIGLVDAGIDTLEALSLRYGADARYPMLLSEFRTAVKLEAAFWHAGRSGAL
CCEEEEHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]