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The map label for this gene is spoT [H]

Identifier: 162147940

GI number: 162147940

Start: 2207922

End: 2210258

Strand: Reverse

Name: spoT [H]

Synonym: GDI_2156

Alternate gene names: 162147940

Gene position: 2210258-2207922 (Counterclockwise)

Preceding gene: 162147941

Following gene: 162147939

Centisome position: 56.04

GC content: 67.52

Gene sequence:

>2337_bases
ATGGGGAAGAATGCGGCGGACCCTGTCGGCACGTTGCATGTCCCCGCCCTCGAACTGGGGGCGTCCCTTCCGGCTGGGGG
CACGGGATCGGGCCGGCCCGTTACCGTGCCTTCGGACCCCGATGCCCCGCCCGCGCCGGCACGGGTCATCTCGTGCGAGG
GATTGCTGCGGCGCATTCGCAGCTACGACCCGGATGCCGACCTCGACCTGATTCGCCGCGCCTTCGCCGTGGCCCAGGCC
GCCCATGCCGGGCAGGTCCGCGACAACGGTGACCCGTACATCACCCATCCGCTGGCGGTTGCCAGTATCCTGGCGGGCTT
CCATCTCGACCCGTCGTCGATCGCGACGGCGCTGCTGCACGATACGATCGAGGATACCGGCGTCACGCACGATCGGCTGC
GCGCCGAATTCGGGCCGACCATCGCCGACCTGGTGGATGGCGTCACCAAGCTGACCCGGCTGGAACTGCAATCCGACCGG
ACCAAGCAGGCCGAGAATTTCCGCAAGCTGGTCCTGGCGATGTCCAAGGACATCCGGGTGCTGATCGTCAAGCTGGCGGA
CCGGCTGCACAACATGCGCACGCTGCATTACGTGCAGCGGCTGGACCGCCGCCAGCGTATCGCGCGCGAGACCATGGAAA
TCTATGCGCCTCTGGCCGGGCGCATCGGCATGGACAAGGTCAAGACCGAACTCCAGAACCTGTCCTTTGCCGAACTGGAG
CCCGAAGCCAACGCCACCATCCGTGCGCGCCTGAACTACCTGCGCGGCCAGGGCGCCGACGTGATCGAGGAAGTGCGGCG
CGAACTCGCGCATCTGTGCCGCGAAGCCGGACTGGGCGAGGTCGAGGTGACGGGGCGCGAGAAATCGCCCTTCTCGATCT
GGGAGAAGATGCAGAACCGCAACGTCGCGTTCGAGCAGCTGTCCGACATCATGGCGTTCCGCATCCTCGTGCCGACGCGT
GAGGCCTGCTACATCGCGCTGGGCGCCGTCCATGCGGCCTATCCGGTCATTGTCGGGCGGTTCAAGGACTATATCTCCAC
CCCCAAGGCGAACGGCTACCAGAGCCTGCATACCGGCGTGACCCTGCGCCAGCCCCGCAACCAGAAGATCGAAGTGCAGA
TCCGCACCGCCGAGATGCACGACATCGCGGAAAACGGCGTTGCCTCGCACTGGCTGTACAAGCAGTTGCCGGGGGCCGGC
GAGGATGCGGCGCGCACGGTCACGGGCCTGCGCTGGGTCCAGGACCTGCTGGATATTCTCGAAGATTCGGCGGCACCCGA
CGAATTCCTCGAAAACACCAAGCTGGAACTCTACCAGGACCAGGTGTTCTGCTTCACCCCGAAAGGGCAGCTGATTTCGC
TGCCGCGCGGGGCCACGCCGGTCGATTTCGCCTATGCCGTGCACAGCCAGGTCGGCGACAGCTGCGTGGGGGCGCGGATC
AACGGGCGGCTGATGCCGCTGCGCCACGAACTGCAGAACGGCGACCAGGTCGAAATCATGACGGCGCGGGGCGGTACGCC
GTCGCCCTCGTGGGAACGCTTCGTCGTCACCGGCAAGGCGCGCGCGCGCATCCGCCGCCATGTCGCGGCCCAGCAGCGCG
AGGCCCATGTCGATGGCGGCCGCGCCGCCCTGGCCAAGGCCTTCCGGCAGGAGGGCGTGGACGGGTCGGAAAAGGTCCTG
GACAGCATCCTGCCCGACCTGAAGCTGGTATCGGTCGAGGATCTGTACGTCGCGGTCGGCAACGGCAATATCAGCCCCCG
CGACGTGGTGCATGCGGCCTATCCCGAGTTGCGGCAGACGCCGCGCGCGCCGCGCATGGTGCCGGGTATTCCCATGCGGG
CGCCGTCGCGGGTGGCCAGCCGGCCGAAGCCGGGCGGCGGCATGGCGCTGGCCGGGATCGGCCCGGGCATCGCGGTGCAT
TTCGCCGGCTGCTGCCATCCGCTGCCGGGCGACAAGATCGTCGGCATCGTCGCGACCGGCAAGGGCATCACCATCCATAT
GGCCGGGTGCCAGACGCTGGAGAGCTTCGCGGCGACCCCCGAACGGTTCATGGACGTGGACTGGGATTACGACATCATCG
GCCGTGGCGCGACCGAACCGCATATCGGGCGGCTGAGCGTGATCGCGGCGAACGAACCGTCCGTGCTGGCGACGCTGACC
AACACCGCGTCGAAGCATGACGGCACCGTCGTCAACCTGCGCATCGTGAACCGCCAGCTCGATTTCATGGAAATCCTGGC
GGATATCGAGGTCCGCGACCTGCGTCACCTCTCCTCGGTGATCGCGGCGCTGCGGGCGGCCAACGGCGTGGTCCAGGTCG
AGCGCGCGAAGGGATGA

Upstream 100 bases:

>100_bases
GCCGAGGAAGAAGCATCCCACGGTACTGCCGGCATGTCGGCCGAAGAACTGGAAGCCGCGATCGAGGCCGAACTGGGCGG
CCGCGCGCGTCGATAAACCC

Downstream 100 bases:

>100_bases
GCCTGATCGGAATCGGATCGGACCTGTGCGATATCAGGCGGATCGAACGCGTGCTGGCCCGTCATGGCGAGCGTTTCCTG
GTCCGCGTCTTCACCGACGC

Product: guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase

Products: NA

Alternate protein names: (p)ppGpp synthase; ATP:GTP 3'-pyrophosphotransferase [H]

Number of amino acids: Translated: 778; Mature: 777

Protein sequence:

>778_residues
MGKNAADPVGTLHVPALELGASLPAGGTGSGRPVTVPSDPDAPPAPARVISCEGLLRRIRSYDPDADLDLIRRAFAVAQA
AHAGQVRDNGDPYITHPLAVASILAGFHLDPSSIATALLHDTIEDTGVTHDRLRAEFGPTIADLVDGVTKLTRLELQSDR
TKQAENFRKLVLAMSKDIRVLIVKLADRLHNMRTLHYVQRLDRRQRIARETMEIYAPLAGRIGMDKVKTELQNLSFAELE
PEANATIRARLNYLRGQGADVIEEVRRELAHLCREAGLGEVEVTGREKSPFSIWEKMQNRNVAFEQLSDIMAFRILVPTR
EACYIALGAVHAAYPVIVGRFKDYISTPKANGYQSLHTGVTLRQPRNQKIEVQIRTAEMHDIAENGVASHWLYKQLPGAG
EDAARTVTGLRWVQDLLDILEDSAAPDEFLENTKLELYQDQVFCFTPKGQLISLPRGATPVDFAYAVHSQVGDSCVGARI
NGRLMPLRHELQNGDQVEIMTARGGTPSPSWERFVVTGKARARIRRHVAAQQREAHVDGGRAALAKAFRQEGVDGSEKVL
DSILPDLKLVSVEDLYVAVGNGNISPRDVVHAAYPELRQTPRAPRMVPGIPMRAPSRVASRPKPGGGMALAGIGPGIAVH
FAGCCHPLPGDKIVGIVATGKGITIHMAGCQTLESFAATPERFMDVDWDYDIIGRGATEPHIGRLSVIAANEPSVLATLT
NTASKHDGTVVNLRIVNRQLDFMEILADIEVRDLRHLSSVIAALRAANGVVQVERAKG

Sequences:

>Translated_778_residues
MGKNAADPVGTLHVPALELGASLPAGGTGSGRPVTVPSDPDAPPAPARVISCEGLLRRIRSYDPDADLDLIRRAFAVAQA
AHAGQVRDNGDPYITHPLAVASILAGFHLDPSSIATALLHDTIEDTGVTHDRLRAEFGPTIADLVDGVTKLTRLELQSDR
TKQAENFRKLVLAMSKDIRVLIVKLADRLHNMRTLHYVQRLDRRQRIARETMEIYAPLAGRIGMDKVKTELQNLSFAELE
PEANATIRARLNYLRGQGADVIEEVRRELAHLCREAGLGEVEVTGREKSPFSIWEKMQNRNVAFEQLSDIMAFRILVPTR
EACYIALGAVHAAYPVIVGRFKDYISTPKANGYQSLHTGVTLRQPRNQKIEVQIRTAEMHDIAENGVASHWLYKQLPGAG
EDAARTVTGLRWVQDLLDILEDSAAPDEFLENTKLELYQDQVFCFTPKGQLISLPRGATPVDFAYAVHSQVGDSCVGARI
NGRLMPLRHELQNGDQVEIMTARGGTPSPSWERFVVTGKARARIRRHVAAQQREAHVDGGRAALAKAFRQEGVDGSEKVL
DSILPDLKLVSVEDLYVAVGNGNISPRDVVHAAYPELRQTPRAPRMVPGIPMRAPSRVASRPKPGGGMALAGIGPGIAVH
FAGCCHPLPGDKIVGIVATGKGITIHMAGCQTLESFAATPERFMDVDWDYDIIGRGATEPHIGRLSVIAANEPSVLATLT
NTASKHDGTVVNLRIVNRQLDFMEILADIEVRDLRHLSSVIAALRAANGVVQVERAKG
>Mature_777_residues
GKNAADPVGTLHVPALELGASLPAGGTGSGRPVTVPSDPDAPPAPARVISCEGLLRRIRSYDPDADLDLIRRAFAVAQAA
HAGQVRDNGDPYITHPLAVASILAGFHLDPSSIATALLHDTIEDTGVTHDRLRAEFGPTIADLVDGVTKLTRLELQSDRT
KQAENFRKLVLAMSKDIRVLIVKLADRLHNMRTLHYVQRLDRRQRIARETMEIYAPLAGRIGMDKVKTELQNLSFAELEP
EANATIRARLNYLRGQGADVIEEVRRELAHLCREAGLGEVEVTGREKSPFSIWEKMQNRNVAFEQLSDIMAFRILVPTRE
ACYIALGAVHAAYPVIVGRFKDYISTPKANGYQSLHTGVTLRQPRNQKIEVQIRTAEMHDIAENGVASHWLYKQLPGAGE
DAARTVTGLRWVQDLLDILEDSAAPDEFLENTKLELYQDQVFCFTPKGQLISLPRGATPVDFAYAVHSQVGDSCVGARIN
GRLMPLRHELQNGDQVEIMTARGGTPSPSWERFVVTGKARARIRRHVAAQQREAHVDGGRAALAKAFRQEGVDGSEKVLD
SILPDLKLVSVEDLYVAVGNGNISPRDVVHAAYPELRQTPRAPRMVPGIPMRAPSRVASRPKPGGGMALAGIGPGIAVHF
AGCCHPLPGDKIVGIVATGKGITIHMAGCQTLESFAATPERFMDVDWDYDIIGRGATEPHIGRLSVIAANEPSVLATLTN
TASKHDGTVVNLRIVNRQLDFMEILADIEVRDLRHLSSVIAALRAANGVVQVERAKG

Specific function: Functions as a (p)ppGpp synthase. In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. Plays a role in ada

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Escherichia coli, GI1790082, Length=741, Percent_Identity=38.191632928475, Blast_Score=507, Evalue=1e-145,
Organism=Escherichia coli, GI1789147, Length=718, Percent_Identity=32.3119777158774, Blast_Score=365, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI25143118, Length=133, Percent_Identity=35.3383458646617, Blast_Score=72, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF01842 ACT; PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =2.7.6.5 [H]

Molecular weight: Translated: 84790; Mature: 84659

Theoretical pI: Translated: 7.56; Mature: 7.56

Prosite motif: PS00227 TUBULIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKNAADPVGTLHVPALELGASLPAGGTGSGRPVTVPSDPDAPPAPARVISCEGLLRRIR
CCCCCCCCCCEEECCHHHHCCCCCCCCCCCCCEEECCCCCCCCCCCCEEEEHHHHHHHHH
SYDPDADLDLIRRAFAVAQAAHAGQVRDNGDPYITHPLAVASILAGFHLDPSSIATALLH
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHCCCCCHHHHHHHHHH
DTIEDTGVTHDRLRAEFGPTIADLVDGVTKLTRLELQSDRTKQAENFRKLVLAMSKDIRV
HHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
LIVKLADRLHNMRTLHYVQRLDRRQRIARETMEIYAPLAGRIGMDKVKTELQNLSFAELE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEECC
PEANATIRARLNYLRGQGADVIEEVRRELAHLCREAGLGEVEVTGREKSPFSIWEKMQNR
CCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHCC
NVAFEQLSDIMAFRILVPTREACYIALGAVHAAYPVIVGRFKDYISTPKANGYQSLHTGV
CCHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCE
TLRQPRNQKIEVQIRTAEMHDIAENGVASHWLYKQLPGAGEDAARTVTGLRWVQDLLDIL
EEECCCCCEEEEEEEECHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
EDSAAPDEFLENTKLELYQDQVFCFTPKGQLISLPRGATPVDFAYAVHSQVGDSCVGARI
HCCCCCHHHHCCCCCEEEECCEEEECCCCCEEECCCCCCCHHHHHHHHHHCCCHHHCCEE
NGRLMPLRHELQNGDQVEIMTARGGTPSPSWERFVVTGKARARIRRHVAAQQREAHVDGG
CCEEEHHHHHHCCCCEEEEEEECCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHCCCH
RAALAKAFRQEGVDGSEKVLDSILPDLKLVSVEDLYVAVGNGNISPRDVVHAAYPELRQT
HHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEEEEEEEEECCCCCCHHHHHHHHCHHHHCC
PRAPRMVPGIPMRAPSRVASRPKPGGGMALAGIGPGIAVHFAGCCHPLPGDKIVGIVATG
CCCCCCCCCCCCCCCHHHHCCCCCCCCEEEECCCCCHHEEECCCCCCCCCCCEEEEEEEC
KGITIHMAGCQTLESFAATPERFMDVDWDYDIIGRGATEPHIGRLSVIAANEPSVLATLT
CCEEEEECCHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEEE
NTASKHDGTVVNLRIVNRQLDFMEILADIEVRDLRHLSSVIAALRAANGVVQVERAKG
CCCCCCCCEEEEEEEECCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEECCCC
>Mature Secondary Structure 
GKNAADPVGTLHVPALELGASLPAGGTGSGRPVTVPSDPDAPPAPARVISCEGLLRRIR
CCCCCCCCCEEECCHHHHCCCCCCCCCCCCCEEECCCCCCCCCCCCEEEEHHHHHHHHH
SYDPDADLDLIRRAFAVAQAAHAGQVRDNGDPYITHPLAVASILAGFHLDPSSIATALLH
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHCCCCCHHHHHHHHHH
DTIEDTGVTHDRLRAEFGPTIADLVDGVTKLTRLELQSDRTKQAENFRKLVLAMSKDIRV
HHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
LIVKLADRLHNMRTLHYVQRLDRRQRIARETMEIYAPLAGRIGMDKVKTELQNLSFAELE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEECC
PEANATIRARLNYLRGQGADVIEEVRRELAHLCREAGLGEVEVTGREKSPFSIWEKMQNR
CCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHCC
NVAFEQLSDIMAFRILVPTREACYIALGAVHAAYPVIVGRFKDYISTPKANGYQSLHTGV
CCHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCE
TLRQPRNQKIEVQIRTAEMHDIAENGVASHWLYKQLPGAGEDAARTVTGLRWVQDLLDIL
EEECCCCCEEEEEEEECHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
EDSAAPDEFLENTKLELYQDQVFCFTPKGQLISLPRGATPVDFAYAVHSQVGDSCVGARI
HCCCCCHHHHCCCCCEEEECCEEEECCCCCEEECCCCCCCHHHHHHHHHHCCCHHHCCEE
NGRLMPLRHELQNGDQVEIMTARGGTPSPSWERFVVTGKARARIRRHVAAQQREAHVDGG
CCEEEHHHHHHCCCCEEEEEEECCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHCCCH
RAALAKAFRQEGVDGSEKVLDSILPDLKLVSVEDLYVAVGNGNISPRDVVHAAYPELRQT
HHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEEEEEEEEECCCCCCHHHHHHHHCHHHHCC
PRAPRMVPGIPMRAPSRVASRPKPGGGMALAGIGPGIAVHFAGCCHPLPGDKIVGIVATG
CCCCCCCCCCCCCCCHHHHCCCCCCCCEEEECCCCCHHEEECCCCCCCCCCCEEEEEEEC
KGITIHMAGCQTLESFAATPERFMDVDWDYDIIGRGATEPHIGRLSVIAANEPSVLATLT
CCEEEEECCHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEEE
NTASKHDGTVVNLRIVNRQLDFMEILADIEVRDLRHLSSVIAALRAANGVVQVERAKG
CCCCCCCCEEEEEEEECCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA