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The map label for this gene is dapE

Identifier: 162147915

GI number: 162147915

Start: 2185227

End: 2186384

Strand: Reverse

Name: dapE

Synonym: GDI_2131

Alternate gene names: 162147915

Gene position: 2186384-2185227 (Counterclockwise)

Preceding gene: 162147916

Following gene: 162147914

Centisome position: 55.43

GC content: 69.26

Gene sequence:

>1158_bases
TTGACTGGTGCCCTGACCCCGGCATCCACCCCCGCGCTCACCCTGGCGCGGGACCTGATCCGCGCGCCGTCGGTCACGCC
GGACGATGGCGGCGCGATCGGGGTGCTGACGGCTGCCCTGCGCGGGCTGGGGTTCGACGTCACCGACCTACCGTTTGGCG
AGGGACCTGCCCGTACCCCGAACCTGTTCGCGCGGCTGGGCCGGTCGGGGCCGCATCTCTGCTTCGCCGGACATACCGAC
GTGGTGCCGCCCGGCGATGGCGGCTGGACCTCGGGCCCGTTCGAGGCCGCGCTGCGGGACGGCTGCCTGTACGGGCGCGG
CGCATGCGACATGAAGGGCGGGATCGCCGCCTTCGTCGGCGCCGTGGCACGGATACTGGAATCGGGCCGGACGCTGCGGG
GTTCGGTCAGCCTGCTGATTACCGGCGACGAGGAAGGACCGGCCACCTTCGGCACCGTCAAGGTGCTGGAATGGATGGCG
GCGCACGGGCAGGTTCCCGATTTCTGCGTGGTGGGCGAACCGACCAATCCCGACCATCTGGGCGATGTCATCAAGATCGG
GCGTCGCGGCAGCCTGAATGCCCGGATCGTCGTGCCGGGAATCCAGGGTCACGTGGCCTATCCCCATCGGGCCGACAATC
CGGTCCATCGCCTGCTGGCCATCCTGTCGGACCTGACGGCCCGCCCGCTGGACCAGGGAACGGAGTGGTTCGAACCGTCC
AGCCTGCAGGTCACCACGGTCGATGTCGGCAACGAGGCGACCAATGTCATTCCCGGCCGGGCCACGGCACGGCTGAACAT
CCGCTTCAACGACCTGCATACCGGCCAGGGCCTGGCCGACTGGATCCGCGGCGTCGCGCATGTCCACGCCCCGGGGGCGG
AGGTCACGGTCCAGATCAGCGGTGAAGCCTTCCGTACCGAACCCACGCCGGAGCTGGACATGCTGGCCGCGTCCATCCAG
GCGGTGACGGGGCGGGCGCCGCGGCTGGATACCGGGGGCGGCACGTCCGATGCACGGTTCATCAGCCGCTATTGCCCGGT
TGCCGAATTCGGCCTGGTCGGGGCCAGCATGCACAAGGTGGACGAGCACGTGCCCGTTGCCGACCTTCTCGCGCTGACCG
ACATCTATGCGGCGTTTCTTGAAAGGCTGATGGGATGA

Upstream 100 bases:

>100_bases
CCGACGGCACGCCGCTGCCCGCGCTGGCCTGCGCCGTGATCGTGAAGCGCGTCGACGAACGCACCCGCTCCAAGACCTCG
ATCAACGAATTGCTGCGCGA

Downstream 100 bases:

>100_bases
TGCAGGGACGCGGCGTGCCGCAGGGACCGTTGCCGAACAGCGCCGGCGGTATCTTTCGCGGCATGATGCTGCTGGGCCGT
GGCCGGCGCGAGGGGCTGAA

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase

Number of amino acids: Translated: 385; Mature: 384

Protein sequence:

>385_residues
MTGALTPASTPALTLARDLIRAPSVTPDDGGAIGVLTAALRGLGFDVTDLPFGEGPARTPNLFARLGRSGPHLCFAGHTD
VVPPGDGGWTSGPFEAALRDGCLYGRGACDMKGGIAAFVGAVARILESGRTLRGSVSLLITGDEEGPATFGTVKVLEWMA
AHGQVPDFCVVGEPTNPDHLGDVIKIGRRGSLNARIVVPGIQGHVAYPHRADNPVHRLLAILSDLTARPLDQGTEWFEPS
SLQVTTVDVGNEATNVIPGRATARLNIRFNDLHTGQGLADWIRGVAHVHAPGAEVTVQISGEAFRTEPTPELDMLAASIQ
AVTGRAPRLDTGGGTSDARFISRYCPVAEFGLVGASMHKVDEHVPVADLLALTDIYAAFLERLMG

Sequences:

>Translated_385_residues
MTGALTPASTPALTLARDLIRAPSVTPDDGGAIGVLTAALRGLGFDVTDLPFGEGPARTPNLFARLGRSGPHLCFAGHTD
VVPPGDGGWTSGPFEAALRDGCLYGRGACDMKGGIAAFVGAVARILESGRTLRGSVSLLITGDEEGPATFGTVKVLEWMA
AHGQVPDFCVVGEPTNPDHLGDVIKIGRRGSLNARIVVPGIQGHVAYPHRADNPVHRLLAILSDLTARPLDQGTEWFEPS
SLQVTTVDVGNEATNVIPGRATARLNIRFNDLHTGQGLADWIRGVAHVHAPGAEVTVQISGEAFRTEPTPELDMLAASIQ
AVTGRAPRLDTGGGTSDARFISRYCPVAEFGLVGASMHKVDEHVPVADLLALTDIYAAFLERLMG
>Mature_384_residues
TGALTPASTPALTLARDLIRAPSVTPDDGGAIGVLTAALRGLGFDVTDLPFGEGPARTPNLFARLGRSGPHLCFAGHTDV
VPPGDGGWTSGPFEAALRDGCLYGRGACDMKGGIAAFVGAVARILESGRTLRGSVSLLITGDEEGPATFGTVKVLEWMAA
HGQVPDFCVVGEPTNPDHLGDVIKIGRRGSLNARIVVPGIQGHVAYPHRADNPVHRLLAILSDLTARPLDQGTEWFEPSS
LQVTTVDVGNEATNVIPGRATARLNIRFNDLHTGQGLADWIRGVAHVHAPGAEVTVQISGEAFRTEPTPELDMLAASIQA
VTGRAPRLDTGGGTSDARFISRYCPVAEFGLVGASMHKVDEHVPVADLLALTDIYAAFLERLMG

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily

Homologues:

Organism=Escherichia coli, GI1788816, Length=379, Percent_Identity=42.2163588390501, Blast_Score=289, Evalue=2e-79,
Organism=Escherichia coli, GI1790395, Length=274, Percent_Identity=28.4671532846715, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1789236, Length=388, Percent_Identity=25.5154639175258, Blast_Score=81, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPE_GLUDA (A9HKR2)

Other databases:

- EMBL:   AM889285
- EMBL:   CP001189
- RefSeq:   YP_001602376.1
- RefSeq:   YP_002274761.1
- ProteinModelPortal:   A9HKR2
- SMR:   A9HKR2
- MEROPS:   M20.010
- GeneID:   5792490
- GeneID:   6973744
- GenomeReviews:   AM889285_GR
- GenomeReviews:   CP001189_GR
- KEGG:   gdj:Gdia_0350
- HOGENOM:   HBG728841
- OMA:   PMVFEDT
- ProtClustDB:   PRK13009
- GO:   GO:0006508
- HAMAP:   MF_01690
- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650
- TIGRFAMs:   TIGR01246

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20; SSF55031 Peptidase_M20_dimer

EC number: =3.5.1.18

Molecular weight: Translated: 40317; Mature: 40186

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS00758 ARGE_DAPE_CPG2_1; PS00759 ARGE_DAPE_CPG2_2; PS01319 RBFA

Important sites: ACT_SITE 80-80 ACT_SITE 144-144

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGALTPASTPALTLARDLIRAPSVTPDDGGAIGVLTAALRGLGFDVTDLPFGEGPARTP
CCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCH
NLFARLGRSGPHLCFAGHTDVVPPGDGGWTSGPFEAALRDGCLYGRGACDMKGGIAAFVG
HHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHCCCEECCCCCCCCCCHHHHHH
AVARILESGRTLRGSVSLLITGDEEGPATFGTVKVLEWMAAHGQVPDFCVVGEPTNPDHL
HHHHHHHCCCEEECEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCHHHH
GDVIKIGRRGSLNARIVVPGIQGHVAYPHRADNPVHRLLAILSDLTARPLDQGTEWFEPS
HHHHHHCCCCCCCCEEEEECCCCCEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCC
SLQVTTVDVGNEATNVIPGRATARLNIRFNDLHTGQGLADWIRGVAHVHAPGAEVTVQIS
CEEEEEEECCCCCCCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHCCCCCCEEEEEEC
GEAFRTEPTPELDMLAASIQAVTGRAPRLDTGGGTSDARFISRYCPVAEFGLVGASMHKV
CCEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHH
DEHVPVADLLALTDIYAAFLERLMG
HHCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TGALTPASTPALTLARDLIRAPSVTPDDGGAIGVLTAALRGLGFDVTDLPFGEGPARTP
CCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCH
NLFARLGRSGPHLCFAGHTDVVPPGDGGWTSGPFEAALRDGCLYGRGACDMKGGIAAFVG
HHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHCCCEECCCCCCCCCCHHHHHH
AVARILESGRTLRGSVSLLITGDEEGPATFGTVKVLEWMAAHGQVPDFCVVGEPTNPDHL
HHHHHHHCCCEEECEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCHHHH
GDVIKIGRRGSLNARIVVPGIQGHVAYPHRADNPVHRLLAILSDLTARPLDQGTEWFEPS
HHHHHHCCCCCCCCEEEEECCCCCEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCC
SLQVTTVDVGNEATNVIPGRATARLNIRFNDLHTGQGLADWIRGVAHVHAPGAEVTVQIS
CEEEEEEECCCCCCCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHCCCCCCEEEEEEC
GEAFRTEPTPELDMLAASIQAVTGRAPRLDTGGGTSDARFISRYCPVAEFGLVGASMHKV
CCEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHH
DEHVPVADLLALTDIYAAFLERLMG
HHCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA