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The map label for this gene is gltD [H]

Identifier: 162147896

GI number: 162147896

Start: 2165886

End: 2167454

Strand: Reverse

Name: gltD [H]

Synonym: GDI_2112

Alternate gene names: 162147896

Gene position: 2167454-2165886 (Counterclockwise)

Preceding gene: 162147897

Following gene: 162147895

Centisome position: 54.95

GC content: 66.6

Gene sequence:

>1569_bases
TTGTCAAAAAGGACAGTCACGCTGTCTTTTTTGACGGTGATGTCTATCGTAGGTCTTCCGCCGGAAACGTGCGCCCGCTT
CGGCAGGAGCGCACGATGTCGCAGGGGGAAGCCGATGGCCGAGCGCATGCTGAAATTCGTCTCCGTGGCGCAGAGCCAGC
CGGACAAGCGCCCGGCTGCCGAACGCAACGCGGATTTCGACGAGATCTATCGCGCATTCGCCGTCGGCAAGGCCGAACAG
CAGGCCAGCCGGTGCTCGCAATGCGGTGTCCCATTCTGCTCGATCCATTGCCCGCTGGGCAACAATATTCCCGACTGGCT
GAAGATGACGGCCGAGGGGCGGCTGGAAGAGGCCTATGCCCTGTCGTCGGCGACGAACAATTTCCCCGAGATCTGCGGCC
GGATCTGCCCCCAGGACCGGCTGTGCGAAGGCAATTGCGTGATCGAGAAGGGGTTCGAGAGCGTCACCATCGGCGCGGTC
GAACGCTTCATCACCGATACCGCCTTCGCCAGCGGCTGGGTCGAGCCGATCCGCCCGCCCGTGGAACGCCAGACCTCGAT
CGGCATCATCGGCGCCGGCCCCGGCGGCCTGGCGGCGGCGATGCAACTGCGCGAGCAGGGGCACCAGGTGCATGTCTATG
ACCGCTACGACCGCGTCGGCGGCCTGATGATCTACGGCATTCCTGGCTTCAAGCTGGAAAAGGACATCGTCGCGCGGCGC
CATCTCTATCTGGAGGAATCGGGGGTGCGGTTCCATCTGGGCCAGAACGTCGCGGACGCGGACGGCGAGGGGCAGGTGTC
GTTCGCGACGCTGGGCGCGCGTCATGACGCGGTGCTGATCGCCACCGGTGTCTACAAGTCGCGCGATATCGGCGGTCCCG
GTGCGGGACTGGCGGGCATCGTCAAGGCGCTGGACTATCTGACGGTGTCGAACCGCCTGTCGCTGGGGGATGATGTGGCG
GCGTCCGAAAGCGCGATGCTGAACGCGAAGGGCAAGTCGGTGGTGGTGATCGGCGGCGGCGATACGGCCATGGATTGCGT
GCGCACCGCCATCCGCCAGGGTGCGAAATCGGTCAAATGCCTCTATCGCCGCGACCGCGCCAACATGCCCGGCTCGATCC
GCGAAGTGAAGAATGCCGAGGAAGAGGGCGTCGAATTCGTCTGGCTGGCCGCGCCCGAGGCGTTCCTGGGCGACGGCACC
GTCACCGGCGTGCGCGCCGTGCGGATGAAGCTGGGCCTGCCCGACGCCAGCGGCCGCCAGTCGGTCGAACCGGTCGAGGA
CAGCTCCTTCGTGCTGGAAGCCGGCCTGGTGATCAAGGCGCTGGGTTTCGATCCCGAGCCGCTGCCCACCCTGTGGGGCC
AGCCGGACCTGGCGGTGTCGCGCTGGGGCACGCTGCGGATCGATCATGAAAGCTTCATGACCAGTCTGCCGGGCGTCTTC
GCGGCGGGTGATATTGTGCGCGGGGCCAGCCTGGTTGTCTGGGCGATCCGCGATGGACGGGATGCCGCGACGCAGATGCA
TCGCTGGCTGGAGAACCGGGCGGCCGCGTGCGCCCAGGCGGCGGAGTGA

Upstream 100 bases:

>100_bases
CATAAATGATCAAAGTCAACGCATTATCTGGCTGATTGGTCCGATACGGACCAATTTTGCGATCTGGTCATCGGGCCGAT
TTGAGGTATCGCCGCGTTTT

Downstream 100 bases:

>100_bases
CGGATATGGACCAGTTTCCCCAGCAGGATGTCACCCCGGACCGGGACTTCGTGGCCGAATGGCACGACAATGCGCGCCGG
ATCGCGGGCCTGTACGATTC

Product: oxidoreductase

Products: NA

Alternate protein names: Glutamate synthase subunit beta; GLTS beta chain; NADPH-GOGAT [H]

Number of amino acids: Translated: 522; Mature: 521

Protein sequence:

>522_residues
MSKRTVTLSFLTVMSIVGLPPETCARFGRSARCRRGKPMAERMLKFVSVAQSQPDKRPAAERNADFDEIYRAFAVGKAEQ
QASRCSQCGVPFCSIHCPLGNNIPDWLKMTAEGRLEEAYALSSATNNFPEICGRICPQDRLCEGNCVIEKGFESVTIGAV
ERFITDTAFASGWVEPIRPPVERQTSIGIIGAGPGGLAAAMQLREQGHQVHVYDRYDRVGGLMIYGIPGFKLEKDIVARR
HLYLEESGVRFHLGQNVADADGEGQVSFATLGARHDAVLIATGVYKSRDIGGPGAGLAGIVKALDYLTVSNRLSLGDDVA
ASESAMLNAKGKSVVVIGGGDTAMDCVRTAIRQGAKSVKCLYRRDRANMPGSIREVKNAEEEGVEFVWLAAPEAFLGDGT
VTGVRAVRMKLGLPDASGRQSVEPVEDSSFVLEAGLVIKALGFDPEPLPTLWGQPDLAVSRWGTLRIDHESFMTSLPGVF
AAGDIVRGASLVVWAIRDGRDAATQMHRWLENRAAACAQAAE

Sequences:

>Translated_522_residues
MSKRTVTLSFLTVMSIVGLPPETCARFGRSARCRRGKPMAERMLKFVSVAQSQPDKRPAAERNADFDEIYRAFAVGKAEQ
QASRCSQCGVPFCSIHCPLGNNIPDWLKMTAEGRLEEAYALSSATNNFPEICGRICPQDRLCEGNCVIEKGFESVTIGAV
ERFITDTAFASGWVEPIRPPVERQTSIGIIGAGPGGLAAAMQLREQGHQVHVYDRYDRVGGLMIYGIPGFKLEKDIVARR
HLYLEESGVRFHLGQNVADADGEGQVSFATLGARHDAVLIATGVYKSRDIGGPGAGLAGIVKALDYLTVSNRLSLGDDVA
ASESAMLNAKGKSVVVIGGGDTAMDCVRTAIRQGAKSVKCLYRRDRANMPGSIREVKNAEEEGVEFVWLAAPEAFLGDGT
VTGVRAVRMKLGLPDASGRQSVEPVEDSSFVLEAGLVIKALGFDPEPLPTLWGQPDLAVSRWGTLRIDHESFMTSLPGVF
AAGDIVRGASLVVWAIRDGRDAATQMHRWLENRAAACAQAAE
>Mature_521_residues
SKRTVTLSFLTVMSIVGLPPETCARFGRSARCRRGKPMAERMLKFVSVAQSQPDKRPAAERNADFDEIYRAFAVGKAEQQ
ASRCSQCGVPFCSIHCPLGNNIPDWLKMTAEGRLEEAYALSSATNNFPEICGRICPQDRLCEGNCVIEKGFESVTIGAVE
RFITDTAFASGWVEPIRPPVERQTSIGIIGAGPGGLAAAMQLREQGHQVHVYDRYDRVGGLMIYGIPGFKLEKDIVARRH
LYLEESGVRFHLGQNVADADGEGQVSFATLGARHDAVLIATGVYKSRDIGGPGAGLAGIVKALDYLTVSNRLSLGDDVAA
SESAMLNAKGKSVVVIGGGDTAMDCVRTAIRQGAKSVKCLYRRDRANMPGSIREVKNAEEEGVEFVWLAAPEAFLGDGTV
TGVRAVRMKLGLPDASGRQSVEPVEDSSFVLEAGLVIKALGFDPEPLPTLWGQPDLAVSRWGTLRIDHESFMTSLPGVFA
AGDIVRGASLVVWAIRDGRDAATQMHRWLENRAAACAQAAE

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=478, Percent_Identity=26.7782426778243, Blast_Score=155, Evalue=6e-38,
Organism=Escherichia coli, GI1789606, Length=478, Percent_Identity=43.5146443514644, Blast_Score=386, Evalue=1e-108,
Organism=Escherichia coli, GI1788811, Length=467, Percent_Identity=44.5396145610278, Blast_Score=379, Evalue=1e-106,
Organism=Escherichia coli, GI87082180, Length=467, Percent_Identity=40.6852248394004, Blast_Score=343, Evalue=1e-95,
Organism=Escherichia coli, GI1788468, Length=452, Percent_Identity=28.0973451327434, Blast_Score=159, Evalue=5e-40,
Organism=Escherichia coli, GI1789243, Length=436, Percent_Identity=32.1100917431193, Blast_Score=133, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17570289, Length=512, Percent_Identity=35.3515625, Blast_Score=267, Evalue=1e-71,
Organism=Caenorhabditis elegans, GI71984108, Length=462, Percent_Identity=25.974025974026, Blast_Score=122, Evalue=6e-28,
Organism=Saccharomyces cerevisiae, GI6320030, Length=505, Percent_Identity=33.0693069306931, Blast_Score=236, Evalue=8e-63,
Organism=Drosophila melanogaster, GI28574881, Length=505, Percent_Identity=36.4356435643564, Blast_Score=278, Evalue=6e-75,
Organism=Drosophila melanogaster, GI24665539, Length=505, Percent_Identity=36.4356435643564, Blast_Score=278, Evalue=6e-75,
Organism=Drosophila melanogaster, GI24665547, Length=505, Percent_Identity=36.4356435643564, Blast_Score=278, Evalue=6e-75,
Organism=Drosophila melanogaster, GI24665543, Length=505, Percent_Identity=36.4356435643564, Blast_Score=278, Evalue=6e-75,
Organism=Drosophila melanogaster, GI24640763, Length=475, Percent_Identity=26.7368421052632, Blast_Score=133, Evalue=4e-31,
Organism=Drosophila melanogaster, GI18858217, Length=475, Percent_Identity=26.7368421052632, Blast_Score=133, Evalue=4e-31,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 56229; Mature: 56098

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKRTVTLSFLTVMSIVGLPPETCARFGRSARCRRGKPMAERMLKFVSVAQSQPDKRPAA
CCCCEEEHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCC
ERNADFDEIYRAFAVGKAEQQASRCSQCGVPFCSIHCPLGNNIPDWLKMTAEGRLEEAYA
CCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHCCCCHHHHHH
LSSATNNFPEICGRICPQDRLCEGNCVIEKGFESVTIGAVERFITDTAFASGWVEPIRPP
HHHCCCCHHHHHHHHCCCCCCCCCCEEEECCCCCEEHHHHHHHHHHHHHHCCCCCCCCCC
VERQTSIGIIGAGPGGLAAAMQLREQGHQVHVYDRYDRVGGLMIYGIPGFKLEKDIVARR
CCCCCCEEEEECCCCHHHHHHHHHHCCCEEEEEECCCCCCCEEEECCCCCCCHHHHHHHH
HLYLEESGVRFHLGQNVADADGEGQVSFATLGARHDAVLIATGVYKSRDIGGPGAGLAGI
HHEEECCCCEEEECCCCCCCCCCCCEEEEEECCCCCEEEEEECCCCCCCCCCCCCCHHHH
VKALDYLTVSNRLSLGDDVAASESAMLNAKGKSVVVIGGGDTAMDCVRTAIRQGAKSVKC
HHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCEEEEECCCCHHHHHHHHHHHHCHHHHHH
LYRRDRANMPGSIREVKNAEEEGVEFVWLAAPEAFLGDGTVTGVRAVRMKLGLPDASGRQ
HHHHHCCCCCCHHHHHHCCHHCCCEEEEEECCHHHCCCCCHHHHHHHHHEECCCCCCCCC
SVEPVEDSSFVLEAGLVIKALGFDPEPLPTLWGQPDLAVSRWGTLRIDHESFMTSLPGVF
CCCCCCCCCEEEECCCEEEEECCCCCCCCCCCCCCCCEEECCCEEEECHHHHHHHCCCHH
AAGDIVRGASLVVWAIRDGRDAATQMHRWLENRAAACAQAAE
HHHHHHCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SKRTVTLSFLTVMSIVGLPPETCARFGRSARCRRGKPMAERMLKFVSVAQSQPDKRPAA
CCCEEEHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCC
ERNADFDEIYRAFAVGKAEQQASRCSQCGVPFCSIHCPLGNNIPDWLKMTAEGRLEEAYA
CCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHCCCCHHHHHH
LSSATNNFPEICGRICPQDRLCEGNCVIEKGFESVTIGAVERFITDTAFASGWVEPIRPP
HHHCCCCHHHHHHHHCCCCCCCCCCEEEECCCCCEEHHHHHHHHHHHHHHCCCCCCCCCC
VERQTSIGIIGAGPGGLAAAMQLREQGHQVHVYDRYDRVGGLMIYGIPGFKLEKDIVARR
CCCCCCEEEEECCCCHHHHHHHHHHCCCEEEEEECCCCCCCEEEECCCCCCCHHHHHHHH
HLYLEESGVRFHLGQNVADADGEGQVSFATLGARHDAVLIATGVYKSRDIGGPGAGLAGI
HHEEECCCCEEEECCCCCCCCCCCCEEEEEECCCCCEEEEEECCCCCCCCCCCCCCHHHH
VKALDYLTVSNRLSLGDDVAASESAMLNAKGKSVVVIGGGDTAMDCVRTAIRQGAKSVKC
HHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCEEEEECCCCHHHHHHHHHHHHCHHHHHH
LYRRDRANMPGSIREVKNAEEEGVEFVWLAAPEAFLGDGTVTGVRAVRMKLGLPDASGRQ
HHHHHCCCCCCHHHHHHCCHHCCCEEEEEECCHHHCCCCCHHHHHHHHHEECCCCCCCCC
SVEPVEDSSFVLEAGLVIKALGFDPEPLPTLWGQPDLAVSRWGTLRIDHESFMTSLPGVF
CCCCCCCCCEEEECCCEEEEECCCCCCCCCCCCCCCCEEECCCEEEECHHHHHHHCCCHH
AAGDIVRGASLVVWAIRDGRDAATQMHRWLENRAAACAQAAE
HHHHHHCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8428988; 2198943 [H]