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The map label for this gene is capD [H]

Identifier: 162147808

GI number: 162147808

Start: 2065890

End: 2067884

Strand: Reverse

Name: capD [H]

Synonym: GDI_2024

Alternate gene names: 162147808

Gene position: 2067884-2065890 (Counterclockwise)

Preceding gene: 162147810

Following gene: 162147807

Centisome position: 52.43

GC content: 70.28

Gene sequence:

>1995_bases
ATGGTCGGGACAATGGCCTCGCAACGCCCTGATCCCCCTCTGCCGGCCGACAGGCGGCGCCCGGTCAACCGCATCGCCGT
CAATGTCCTGCTGGACGGCCTTCTCGCCGCCCTGTCGGCGCCGCTGGCACGCTGGCTGGCCGACCCGCGCGGCGGCCTGC
TGCATCCGCTCTGGTTCGTCGCGGGCGGCGCCATCACCCTGATCATCAGCGGCCTGCCGTTTCGTGTGCAGCAGCAATAT
TGGCGGTTTTCGGGGGTGGGCGACCTGCTGAACATCGCGGGTGCCTCGGTGGCCAGCGCCATCCTGTTCGCGCTGGGCCT
GCATATCACCGGCTTTCCGATCCCGACCCCGACCTTTCCCATCATCCATGCCCTGGTGCTGCTGACGCTGATGGGCGGGA
TACGGATCATCTACCGGCTATCCTATCGCCGCACGGCGCGGCGGCAGGCATCCAGCCAGGTGATCCTGGTCGGCTCGGAC
AATTCGGCCGACCTGTTCATCCGCGCGGTCGAACGCGCGCCCGATCCCGCCTTTCGCGTGGCCGGCATCGTCACCCAGGG
GCGGCGGCAGGCCGGGCGGCGCATCCACGGCATTCCCATCCTGGGCCATGTCGAGGGAATGGACGACATCCTGGCCCGCC
TGGACGGGCATGGCGGCCGGCCCGAGCGGCTGGTCATCACCGATGCCGCGTTCCGGGGCGAGGACCTGGCCGAGATCATG
CAGGTGGCCGAGCGGCGGGGACTGACCGTCCTGCGGGCGCCGGTCCTGACCGACCTGACGCCCGCCGACCGGCTGGAACT
GCGCCCCGTCGCGATCGAGGACCTGCTGAACCGTCCCCAGGTCGGGCTGGACCATGACGGCATGGCGGCCCTGCTGGGCG
GCCGCACCGTCCTGGTGACCGGGGCCGGCGGCTCGATCGGGTCGGAACTGGCGCGGCAGATCGCGGAATACGAGCCCGGC
CGCCTGCTGCTGCTGGACCATGGCGAATTCGCGCTGTGGCAGATCGACGTGGAACTGTCGGAACGCGCGCCCCGGCTGCG
GCGCGAGACGATCCTGGCCGATATCCGCGACCGGGCGCGGATCGAACAGGTCTGCGCGCAGTACCGTCCCGAACTGGTGT
TCCACGCGGCGGCACTGAAACACGTGCCGATGGTCGAGGCCAATCCGTGCGAGGGGGTGCTGACGAACGTCATCGGCACC
CGGATCGTCGCGGACGCGGCGGCGCGGCATGGCGCCCGCGCCCTGGTCATGGTCTCGACGGACAAGGCGGTGAATCCGTC
CAGCCTGATGGGGGCCTCCAAGCGCGCGGCCGAGATGTATTGCCAGGCCCTGGATGTCGAGGCCCGCACCGGCGACGAAT
CCGGCGTGATGCGGTGCGTCACCGTACGGTTCGGCAACGTCCTGGGTTCGACGGGATCGGTGGTGCCCCTGTTCCGCCGG
CAACTGGAACGGGGCGGGCCGCTGACGGTTACCCACCCCGACATGCAGCGCTATTTCATGACGGTGTCCGAGGCCGTGGG
GCTGGTGCTGCAGGCCAGCGTGCGCGGCACCATCCGCGCCCGCGCGGGCAGCGGCACGGACACGCTGCTGCGCAGCGGCG
GCATCTTCGTCCTGGACATGGGCAAGCCGATCCGGATCGTCGACCTGGCGCGGCAGATGATCCGCCTGGCCGGCCTGCGC
CCCGACCAGGACGTGCCGATCCGCTTCACCGGCCTGCGTCCGGGGGAAAAGCTGTTCGAGGAACTGTTCCATGGGCGCGA
GGCCCCGGTCGCGACCGACCATCCGGGCCTGAAGATGGCGACCCCCCGCATGGTGGACCGTGGGGCCGTCGGCCTGGCGA
TCGACACGCTGGAAGCCGCCTGCCGCGCCGAGGATACTCCGGCCGTCCTTGCGCTGATCGGGAGCCTCGTTCCCGAATTC
GCCCATAACCCGACCGGCGACGTGCGCGATATGCTCGCGGACCCGACCGACGCCGAAAGGACCATGACCCCATGA

Upstream 100 bases:

>100_bases
CAGAAAAGCGAACGGGACGGAGGGGGGAAAAACGAAATGGTTCATGCGCCAGGGGTTTTTGTCGTCATGACTCTATCTAG
CACGGAATGGGCTGGGCTAT

Downstream 100 bases:

>100_bases
CCGCCTCCCCCGAGACGCTTTCCCCCATCGCGTTCCTCGACCTGCCCGCGCAGCAGGCGCGGCTGGGCGACGCCATCCGG
CGGCGGGTGGATGCCGTCAT

Product: capsular polysaccharide biosynthesis protein capD

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 664; Mature: 664

Protein sequence:

>664_residues
MVGTMASQRPDPPLPADRRRPVNRIAVNVLLDGLLAALSAPLARWLADPRGGLLHPLWFVAGGAITLIISGLPFRVQQQY
WRFSGVGDLLNIAGASVASAILFALGLHITGFPIPTPTFPIIHALVLLTLMGGIRIIYRLSYRRTARRQASSQVILVGSD
NSADLFIRAVERAPDPAFRVAGIVTQGRRQAGRRIHGIPILGHVEGMDDILARLDGHGGRPERLVITDAAFRGEDLAEIM
QVAERRGLTVLRAPVLTDLTPADRLELRPVAIEDLLNRPQVGLDHDGMAALLGGRTVLVTGAGGSIGSELARQIAEYEPG
RLLLLDHGEFALWQIDVELSERAPRLRRETILADIRDRARIEQVCAQYRPELVFHAAALKHVPMVEANPCEGVLTNVIGT
RIVADAAARHGARALVMVSTDKAVNPSSLMGASKRAAEMYCQALDVEARTGDESGVMRCVTVRFGNVLGSTGSVVPLFRR
QLERGGPLTVTHPDMQRYFMTVSEAVGLVLQASVRGTIRARAGSGTDTLLRSGGIFVLDMGKPIRIVDLARQMIRLAGLR
PDQDVPIRFTGLRPGEKLFEELFHGREAPVATDHPGLKMATPRMVDRGAVGLAIDTLEAACRAEDTPAVLALIGSLVPEF
AHNPTGDVRDMLADPTDAERTMTP

Sequences:

>Translated_664_residues
MVGTMASQRPDPPLPADRRRPVNRIAVNVLLDGLLAALSAPLARWLADPRGGLLHPLWFVAGGAITLIISGLPFRVQQQY
WRFSGVGDLLNIAGASVASAILFALGLHITGFPIPTPTFPIIHALVLLTLMGGIRIIYRLSYRRTARRQASSQVILVGSD
NSADLFIRAVERAPDPAFRVAGIVTQGRRQAGRRIHGIPILGHVEGMDDILARLDGHGGRPERLVITDAAFRGEDLAEIM
QVAERRGLTVLRAPVLTDLTPADRLELRPVAIEDLLNRPQVGLDHDGMAALLGGRTVLVTGAGGSIGSELARQIAEYEPG
RLLLLDHGEFALWQIDVELSERAPRLRRETILADIRDRARIEQVCAQYRPELVFHAAALKHVPMVEANPCEGVLTNVIGT
RIVADAAARHGARALVMVSTDKAVNPSSLMGASKRAAEMYCQALDVEARTGDESGVMRCVTVRFGNVLGSTGSVVPLFRR
QLERGGPLTVTHPDMQRYFMTVSEAVGLVLQASVRGTIRARAGSGTDTLLRSGGIFVLDMGKPIRIVDLARQMIRLAGLR
PDQDVPIRFTGLRPGEKLFEELFHGREAPVATDHPGLKMATPRMVDRGAVGLAIDTLEAACRAEDTPAVLALIGSLVPEF
AHNPTGDVRDMLADPTDAERTMTP
>Mature_664_residues
MVGTMASQRPDPPLPADRRRPVNRIAVNVLLDGLLAALSAPLARWLADPRGGLLHPLWFVAGGAITLIISGLPFRVQQQY
WRFSGVGDLLNIAGASVASAILFALGLHITGFPIPTPTFPIIHALVLLTLMGGIRIIYRLSYRRTARRQASSQVILVGSD
NSADLFIRAVERAPDPAFRVAGIVTQGRRQAGRRIHGIPILGHVEGMDDILARLDGHGGRPERLVITDAAFRGEDLAEIM
QVAERRGLTVLRAPVLTDLTPADRLELRPVAIEDLLNRPQVGLDHDGMAALLGGRTVLVTGAGGSIGSELARQIAEYEPG
RLLLLDHGEFALWQIDVELSERAPRLRRETILADIRDRARIEQVCAQYRPELVFHAAALKHVPMVEANPCEGVLTNVIGT
RIVADAAARHGARALVMVSTDKAVNPSSLMGASKRAAEMYCQALDVEARTGDESGVMRCVTVRFGNVLGSTGSVVPLFRR
QLERGGPLTVTHPDMQRYFMTVSEAVGLVLQASVRGTIRARAGSGTDTLLRSGGIFVLDMGKPIRIVDLARQMIRLAGLR
PDQDVPIRFTGLRPGEKLFEELFHGREAPVATDHPGLKMATPRMVDRGAVGLAIDTLEAACRAEDTPAVLALIGSLVPEF
AHNPTGDVRDMLADPTDAERTMTP

Specific function: Involved in biofilm formation [H]

COG id: COG1086

COG function: function code MG; Predicted nucleoside-diphosphate sugar epimerases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR003869 [H]

Pfam domain/function: PF02719 Polysacc_synt_2 [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 71764; Mature: 71764

Theoretical pI: Translated: 8.73; Mature: 8.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVGTMASQRPDPPLPADRRRPVNRIAVNVLLDGLLAALSAPLARWLADPRGGLLHPLWFV
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
AGGAITLIISGLPFRVQQQYWRFSGVGDLLNIAGASVASAILFALGLHITGFPIPTPTFP
HCCEEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
IIHALVLLTLMGGIRIIYRLSYRRTARRQASSQVILVGSDNSADLFIRAVERAPDPAFRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHEEHHHCCCHHHHH
AGIVTQGRRQAGRRIHGIPILGHVEGMDDILARLDGHGGRPERLVITDAAFRGEDLAEIM
HHHHHHHHHHHCCEEECCEEEECCCCHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHH
QVAERRGLTVLRAPVLTDLTPADRLELRPVAIEDLLNRPQVGLDHDGMAALLGGRTVLVT
HHHHHCCCEEEECCHHCCCCCCCCCEECCCHHHHHHCCCCCCCCCCCHHHEECCCEEEEE
GAGGSIGSELARQIAEYEPGRLLLLDHGEFALWQIDVELSERAPRLRRETILADIRDRAR
CCCCCHHHHHHHHHHHCCCCEEEEEECCCEEEEEEEEEHHHCCHHHHHHHHHHHHHHHHH
IEQVCAQYRPELVFHAAALKHVPMVEANPCEGVLTNVIGTRIVADAAARHGARALVMVST
HHHHHHHHCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEC
DKAVNPSSLMGASKRAAEMYCQALDVEARTGDESGVMRCVTVRFGNVLGSTGSVVPLFRR
CCCCCHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEHHHHHHCCCCCCHHHHHH
QLERGGPLTVTHPDMQRYFMTVSEAVGLVLQASVRGTIRARAGSGTDTLLRSGGIFVLDM
HHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHCCCEEEEEC
GKPIRIVDLARQMIRLAGLRPDQDVPIRFTGLRPGEKLFEELFHGREAPVATDHPGLKMA
CCCCHHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCEEC
TPRMVDRGAVGLAIDTLEAACRAEDTPAVLALIGSLVPEFAHNPTGDVRDMLADPTDAER
CCHHHCCCCCEEEHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCC
TMTP
CCCC
>Mature Secondary Structure
MVGTMASQRPDPPLPADRRRPVNRIAVNVLLDGLLAALSAPLARWLADPRGGLLHPLWFV
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
AGGAITLIISGLPFRVQQQYWRFSGVGDLLNIAGASVASAILFALGLHITGFPIPTPTFP
HCCEEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
IIHALVLLTLMGGIRIIYRLSYRRTARRQASSQVILVGSDNSADLFIRAVERAPDPAFRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHEEHHHCCCHHHHH
AGIVTQGRRQAGRRIHGIPILGHVEGMDDILARLDGHGGRPERLVITDAAFRGEDLAEIM
HHHHHHHHHHHCCEEECCEEEECCCCHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHH
QVAERRGLTVLRAPVLTDLTPADRLELRPVAIEDLLNRPQVGLDHDGMAALLGGRTVLVT
HHHHHCCCEEEECCHHCCCCCCCCCEECCCHHHHHHCCCCCCCCCCCHHHEECCCEEEEE
GAGGSIGSELARQIAEYEPGRLLLLDHGEFALWQIDVELSERAPRLRRETILADIRDRAR
CCCCCHHHHHHHHHHHCCCCEEEEEECCCEEEEEEEEEHHHCCHHHHHHHHHHHHHHHHH
IEQVCAQYRPELVFHAAALKHVPMVEANPCEGVLTNVIGTRIVADAAARHGARALVMVST
HHHHHHHHCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEC
DKAVNPSSLMGASKRAAEMYCQALDVEARTGDESGVMRCVTVRFGNVLGSTGSVVPLFRR
CCCCCHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEHHHHHHCCCCCCHHHHHH
QLERGGPLTVTHPDMQRYFMTVSEAVGLVLQASVRGTIRARAGSGTDTLLRSGGIFVLDM
HHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHCCCEEEEEC
GKPIRIVDLARQMIRLAGLRPDQDVPIRFTGLRPGEKLFEELFHGREAPVATDHPGLKMA
CCCCHHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCEEC
TPRMVDRGAVGLAIDTLEAACRAEDTPAVLALIGSLVPEFAHNPTGDVRDMLADPTDAER
CCHHHCCCCCEEEHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCC
TMTP
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.093 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: p-Chloromercuribenzoate; TMP [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969506; 9384377 [H]