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The map label for this gene is gph [C]

Identifier: 162147770

GI number: 162147770

Start: 2018620

End: 2019327

Strand: Direct

Name: gph [C]

Synonym: GDI_1986

Alternate gene names: 162147770

Gene position: 2018620-2019327 (Clockwise)

Preceding gene: 162147767

Following gene: 162147772

Centisome position: 51.18

GC content: 64.97

Gene sequence:

>708_bases
ATGAATGTCCTGTCGTCCTTGAACCGTCACGATGCGGTTCTTCTTGATCTCGATGGCACGATCGTCGATTCTCGTGCCGG
AATTATCGGCTCCCTGCACGATTCGTTGTGCGATCTTGGGCATGAACCCGATCCCGAGGAAGACCTGACCTGGGTAGTCG
GCCCCCCGCTGCACGATCTGGTCGCCCGGGTCTTGCAACATTATGGCGACGACCGGGCCGAAGAGGCTGTCCGACTCTAT
CGCTATCACTACGAACGCCACGGCATGCATCGCAGTGCCGTGTTCGCCGGCATGCAGGATGTCATCGAAACGGTGGCGGC
ATCCGGCGCGCGGCTGTTCCTGGCGACGTCCAAGCCGGTGCATCTGGCGCGCGCGATCCTGGATCTGCGCGGGCTGACGA
AATATTTCACCGCCTTGTACGGCGCGCGGCCCGATGACAGCGGGGCGGAAAAACCGGAACTGATCGGGCAGTTGCTGCGC
GAGCACGCAATTGCGGCCGGCCGCGCCGTCATGGTCGGCGACCGTCGCTACGACATCAGCGGGGCGCATGCGAATCATGT
CCGGGCGCTGGGCGCGCTTTGGGGCTATGGCGGCGAGGAAGAACTGACCGAGGCCGGGGCGGATGCCCTGGTGGCCGATC
CCGGCGAGCTTCTGGCCGCGATCCGCAGCCAGCTTGCGGCGGCGCACCTGCACCCCGCTGCGGAATGA

Upstream 100 bases:

>100_bases
TCCCGCCTGGTACAGGGCCGGATGCCTCGGGTGACGGTCGCAACGGCTCGATTGCGTGCGTATATAGAATACTCATGGAG
CCTAAAGGATTATCGCCGGT

Downstream 100 bases:

>100_bases
CGGCCATCCTATAGCCGCCGCTGCTGCCGCAGGCTGAGCCACTGGCCATGGTGCAGGATCAGGTGGTCCTGCACCGTCAG
GGACAGGTTCTGCGCATGGT

Product: phosphoglycolate phosphatase

Products: NA

Alternate protein names: Nucleoside 5'-monophosphate phosphohydrolase [H]

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MNVLSSLNRHDAVLLDLDGTIVDSRAGIIGSLHDSLCDLGHEPDPEEDLTWVVGPPLHDLVARVLQHYGDDRAEEAVRLY
RYHYERHGMHRSAVFAGMQDVIETVAASGARLFLATSKPVHLARAILDLRGLTKYFTALYGARPDDSGAEKPELIGQLLR
EHAIAAGRAVMVGDRRYDISGAHANHVRALGALWGYGGEEELTEAGADALVADPGELLAAIRSQLAAAHLHPAAE

Sequences:

>Translated_235_residues
MNVLSSLNRHDAVLLDLDGTIVDSRAGIIGSLHDSLCDLGHEPDPEEDLTWVVGPPLHDLVARVLQHYGDDRAEEAVRLY
RYHYERHGMHRSAVFAGMQDVIETVAASGARLFLATSKPVHLARAILDLRGLTKYFTALYGARPDDSGAEKPELIGQLLR
EHAIAAGRAVMVGDRRYDISGAHANHVRALGALWGYGGEEELTEAGADALVADPGELLAAIRSQLAAAHLHPAAE
>Mature_235_residues
MNVLSSLNRHDAVLLDLDGTIVDSRAGIIGSLHDSLCDLGHEPDPEEDLTWVVGPPLHDLVARVLQHYGDDRAEEAVRLY
RYHYERHGMHRSAVFAGMQDVIETVAASGARLFLATSKPVHLARAILDLRGLTKYFTALYGARPDDSGAEKPELIGQLLR
EHAIAAGRAVMVGDRRYDISGAHANHVRALGALWGYGGEEELTEAGADALVADPGELLAAIRSQLAAAHLHPAAE

Specific function: Specifically dephosphorylates nucleoside 5'- monophosphates to nucleosides and inorganic phosphate. Displays high activity toward 5'-UMP and 5'-IMP, significant activity against 5'-XMP and 5'-TMP, and low activity against 5'-CMP [H]

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=232, Percent_Identity=27.5862068965517, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.1.3.5 [H]

Molecular weight: Translated: 25384; Mature: 25384

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVLSSLNRHDAVLLDLDGTIVDSRAGIIGSLHDSLCDLGHEPDPEEDLTWVVGPPLHDL
CCHHHHCCCCCEEEEECCCEEECCCCCHHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHH
VARVLQHYGDDRAEEAVRLYRYHYERHGMHRSAVFAGMQDVIETVAASGARLFLATSKPV
HHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCHH
HLARAILDLRGLTKYFTALYGARPDDSGAEKPELIGQLLREHAIAAGRAVMVGDRRYDIS
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCEECCC
GAHANHVRALGALWGYGGEEELTEAGADALVADPGELLAAIRSQLAAAHLHPAAE
CCCHHHHHHHHHHHCCCCHHHHHHCCCCCEECCHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNVLSSLNRHDAVLLDLDGTIVDSRAGIIGSLHDSLCDLGHEPDPEEDLTWVVGPPLHDL
CCHHHHCCCCCEEEEECCCEEECCCCCHHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHH
VARVLQHYGDDRAEEAVRLYRYHYERHGMHRSAVFAGMQDVIETVAASGARLFLATSKPV
HHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCHH
HLARAILDLRGLTKYFTALYGARPDDSGAEKPELIGQLLREHAIAAGRAVMVGDRRYDIS
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCEECCC
GAHANHVRALGALWGYGGEEELTEAGADALVADPGELLAAIRSQLAAAHLHPAAE
CCCHHHHHHHHHHHCCCCHHHHHHCCCCCEECCHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]