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The map label for this gene is purB [H]

Identifier: 162147738

GI number: 162147738

Start: 1989915

End: 1991270

Strand: Direct

Name: purB [H]

Synonym: GDI_1954

Alternate gene names: 162147738

Gene position: 1989915-1991270 (Clockwise)

Preceding gene: 162147729

Following gene: 162147739

Centisome position: 50.45

GC content: 69.54

Gene sequence:

>1356_bases
ATGGTTCCGCGTTATACCCGCCCCGCCATGGCCGCCATCTGGGCCCCCGAGAACCGGTACCGGATCTGGTTCGAGATCGA
GGCCCTGGCGTGCGAGGCCATGGCGCAGTACGGCGCCGTGCCCGCCGAGGCCGCGAAAGTGGTGCGGGAGAAGGGCGACG
CCGCCATGGCCGCGTTCTCGCAGGCCGACCTGGACCGCATCGACGAGATCGAGGCCGAGACCCGGCACGACGTCATCGCG
TTCCTGACCTGGCTGGCCGAGAAGATCGGCCCCGAAAGCCGCTTCGTCCATCTGGGCATGACGTCGTCCGACGTGCTGGA
CACCTGCCTGTCGGTGCAGCTGGTGCAGGCCACCGACATGCTGCTGGCGGACCTGGATGCCGTGCTGGACGCGCTGAAGC
GCCGGGCGTTCGAGCATAAATACACGCTGACCATCGGGCGCAGCCACGCGATCCATGCCGAACCGACCTCGTTCGGGCTG
AAGCTGGCCGGCCATTACGCCGAATTCGCCCGCAATCGCGAGCGGCTGGTCCGCGCCCGCGCGGAAATCGCCACCTGCGC
CATTTCCGGCGCGGTCGGCACCTATGCCCATGTCGACCCCCGGGTCGAGGAATTCGTGGCCGCGCGGCTGGGGCTGGAGC
CCGAGGGCGTGTCCACCCAGGTGATCCCGCGCGACCGCCATGCCGCCTATTTCTGCGCGCTGGCGGTGATCGCCAGCGGC
ATCGAGCGCCTGGCGGTCGAGGTCCGGCACCTGCAGCGTTCGGAAGTGCGCGAGGCCGAGGAGTTCTTCCATCCGGGCCA
GAAGGGTTCGTCGGCGATGCCGCACAAGCGGAACCCGGTCCTGTCGGAAAACCTGACCGGCCTGGCCCGCCTGGTGCGCG
CGCATGTCATCCCGGCGCTGGAGAACGTGGCGCTGTGGCACGAGCGCGACATCAGCCATTCGTCGGTCGAGCGCAACATC
TGCCCCGACGGCACGATCGGCCTGGATTTCGCCCTGGCCCGGCTGGCCGGCATGATGGACAAGCTGGTGATCTATCCCGA
CCGGATGATCGCGAACCTGGAAAGCCTGGGCGGCGTTGTCCATTCCGGCGAGGTCCTGCTGGCCCTGGCCCGTGCCGGCA
TCCTGCGCGAGGATGCGTACCGCATCGTCCAGCGCAACGCGATGGCGACCTGGACCATGCTGGGCAAGCCCGGCGGCCGC
TCGTTCCGCGAGAACCTGGAAGCCGACCCCGAGGTCGCGGGCCGGGTCCCGGCCTCGGTGCTGGACGCCGCCATGAACAG
CGATTCCCACCTGGGCGCGCTGGACCACAGCTATACCCGCGTGTTCGGCGAAGCCGGGCCCGGCCGGGCGGGCTGA

Upstream 100 bases:

>100_bases
TTTCCAAGGGAGACGCGGCGGCGGAAATCGGCTAAAGGGGCGACCCCGTCCTTCATTCGCCGCCCCCTCCTTTTCATCGA
TAGAGAGAAAGCCGAGCACG

Downstream 100 bases:

>100_bases
TACACGGCACCCGCGCGCCGGGGCCGTGACGCAAGGATGGAAGAAAGATCGTGTGTACCATCGTCCTGTCCCTGGCCCCC
GGTTCCGAATGGCCCCTGCT

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase; Glutamyl--tRNA ligase regulatory factor [H]

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MVPRYTRPAMAAIWAPENRYRIWFEIEALACEAMAQYGAVPAEAAKVVREKGDAAMAAFSQADLDRIDEIEAETRHDVIA
FLTWLAEKIGPESRFVHLGMTSSDVLDTCLSVQLVQATDMLLADLDAVLDALKRRAFEHKYTLTIGRSHAIHAEPTSFGL
KLAGHYAEFARNRERLVRARAEIATCAISGAVGTYAHVDPRVEEFVAARLGLEPEGVSTQVIPRDRHAAYFCALAVIASG
IERLAVEVRHLQRSEVREAEEFFHPGQKGSSAMPHKRNPVLSENLTGLARLVRAHVIPALENVALWHERDISHSSVERNI
CPDGTIGLDFALARLAGMMDKLVIYPDRMIANLESLGGVVHSGEVLLALARAGILREDAYRIVQRNAMATWTMLGKPGGR
SFRENLEADPEVAGRVPASVLDAAMNSDSHLGALDHSYTRVFGEAGPGRAG

Sequences:

>Translated_451_residues
MVPRYTRPAMAAIWAPENRYRIWFEIEALACEAMAQYGAVPAEAAKVVREKGDAAMAAFSQADLDRIDEIEAETRHDVIA
FLTWLAEKIGPESRFVHLGMTSSDVLDTCLSVQLVQATDMLLADLDAVLDALKRRAFEHKYTLTIGRSHAIHAEPTSFGL
KLAGHYAEFARNRERLVRARAEIATCAISGAVGTYAHVDPRVEEFVAARLGLEPEGVSTQVIPRDRHAAYFCALAVIASG
IERLAVEVRHLQRSEVREAEEFFHPGQKGSSAMPHKRNPVLSENLTGLARLVRAHVIPALENVALWHERDISHSSVERNI
CPDGTIGLDFALARLAGMMDKLVIYPDRMIANLESLGGVVHSGEVLLALARAGILREDAYRIVQRNAMATWTMLGKPGGR
SFRENLEADPEVAGRVPASVLDAAMNSDSHLGALDHSYTRVFGEAGPGRAG
>Mature_451_residues
MVPRYTRPAMAAIWAPENRYRIWFEIEALACEAMAQYGAVPAEAAKVVREKGDAAMAAFSQADLDRIDEIEAETRHDVIA
FLTWLAEKIGPESRFVHLGMTSSDVLDTCLSVQLVQATDMLLADLDAVLDALKRRAFEHKYTLTIGRSHAIHAEPTSFGL
KLAGHYAEFARNRERLVRARAEIATCAISGAVGTYAHVDPRVEEFVAARLGLEPEGVSTQVIPRDRHAAYFCALAVIASG
IERLAVEVRHLQRSEVREAEEFFHPGQKGSSAMPHKRNPVLSENLTGLARLVRAHVIPALENVALWHERDISHSSVERNI
CPDGTIGLDFALARLAGMMDKLVIYPDRMIANLESLGGVVHSGEVLLALARAGILREDAYRIVQRNAMATWTMLGKPGGR
SFRENLEADPEVAGRVPASVLDAAMNSDSHLGALDHSYTRVFGEAGPGRAG

Specific function: Influences the affinity of glutamyl--tRNA ligase for its substrates and increases its thermostability [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=411, Percent_Identity=24.5742092457421, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI183227688, Length=403, Percent_Identity=24.8138957816377, Blast_Score=115, Evalue=6e-26,
Organism=Escherichia coli, GI1787376, Length=379, Percent_Identity=30.8707124010554, Blast_Score=107, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17508577, Length=403, Percent_Identity=23.0769230769231, Blast_Score=87, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI32564234, Length=338, Percent_Identity=24.5562130177515, Blast_Score=75, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6323391, Length=361, Percent_Identity=26.8698060941828, Blast_Score=107, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24647570, Length=412, Percent_Identity=25, Blast_Score=92, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 49395; Mature: 49395

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVPRYTRPAMAAIWAPENRYRIWFEIEALACEAMAQYGAVPAEAAKVVREKGDAAMAAFS
CCCCCCCCCHHEEECCCCCEEEEEEEHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHH
QADLDRIDEIEAETRHDVIAFLTWLAEKIGPESRFVHLGMTSSDVLDTCLSVQLVQATDM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHH
LLADLDAVLDALKRRAFEHKYTLTIGRSHAIHAEPTSFGLKLAGHYAEFARNRERLVRAR
HHHHHHHHHHHHHHHHHCCEEEEEECCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
AEIATCAISGAVGTYAHVDPRVEEFVAARLGLEPEGVSTQVIPRDRHAAYFCALAVIASG
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHHH
IERLAVEVRHLQRSEVREAEEFFHPGQKGSSAMPHKRNPVLSENLTGLARLVRAHVIPAL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
ENVALWHERDISHSSVERNICPDGTIGLDFALARLAGMMDKLVIYPDRMIANLESLGGVV
HHHHHHHHCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCC
HSGEVLLALARAGILREDAYRIVQRNAMATWTMLGKPGGRSFRENLEADPEVAGRVPASV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHCCCCCCHHHCCCCHHH
LDAAMNSDSHLGALDHSYTRVFGEAGPGRAG
HHHHHCCCCCCCCHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MVPRYTRPAMAAIWAPENRYRIWFEIEALACEAMAQYGAVPAEAAKVVREKGDAAMAAFS
CCCCCCCCCHHEEECCCCCEEEEEEEHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHH
QADLDRIDEIEAETRHDVIAFLTWLAEKIGPESRFVHLGMTSSDVLDTCLSVQLVQATDM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHH
LLADLDAVLDALKRRAFEHKYTLTIGRSHAIHAEPTSFGLKLAGHYAEFARNRERLVRAR
HHHHHHHHHHHHHHHHHCCEEEEEECCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
AEIATCAISGAVGTYAHVDPRVEEFVAARLGLEPEGVSTQVIPRDRHAAYFCALAVIASG
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHHH
IERLAVEVRHLQRSEVREAEEFFHPGQKGSSAMPHKRNPVLSENLTGLARLVRAHVIPAL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
ENVALWHERDISHSSVERNICPDGTIGLDFALARLAGMMDKLVIYPDRMIANLESLGGVV
HHHHHHHHCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCC
HSGEVLLALARAGILREDAYRIVQRNAMATWTMLGKPGGRSFRENLEADPEVAGRVPASV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHCCCCCCHHHCCCCHHH
LDAAMNSDSHLGALDHSYTRVFGEAGPGRAG
HHHHHCCCCCCCCHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377; 1608947; 10926519 [H]