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The map label for this gene is slt [H]

Identifier: 162147736

GI number: 162147736

Start: 1987061

End: 1989082

Strand: Reverse

Name: slt [H]

Synonym: GDI_1952

Alternate gene names: 162147736

Gene position: 1989082-1987061 (Counterclockwise)

Preceding gene: 162147737

Following gene: 162147735

Centisome position: 50.43

GC content: 72.35

Gene sequence:

>2022_bases
ATGAGGCGTCTTCTCCCCTTCTTTCCCCTTCCCGTTCTCGCCTGCCTGGCGCTGCTGGGCGGGTCGGCGCGTGCCGATTC
ATCCGGCCTGCCGGAACCGCCCCGGTCGGTGCCGTCCGATGCGGTCGTCCCCCCTGCGGTGCCGGATGGGACATCGGATG
CGGTCGCGGCGCGGGTGGATACGTATCTGCGCCTGCTCTCGCCCCTTGGTGGCGATATCGACGAGTACCTGTCCTTCCTG
GACCAGACCCCGACCTGGCCGCGCCGCGCGGTCATGCTGGGCCGCCTGCAACGGCTGATGGCGGTGGCGGCCCCGGGCGC
GCCCGGGATGGCGCAGGCCTGCGCCACCCTGACCCTGACCTATGCCCCCGCCCTGGCGGCCTGTGCCCGCCAGCCGGTTC
CCGCCCCCGACCTGCCGGCCCGCGCGCGCCATGCCTGGACCGAGGGCGTCGACCAGATGGAGGACGAAGCCGCGTTTCTG
GCCGCGTTCGCCCCCGTCCTGACCCCGGCCGACCAATGGCATCGCTTCATGCGGCAGGAACTGACGGGGCATCTGGATGC
CGCCCAGCGACAGGTGGCGCGCGTGGATATCGGCCGTCAGGCCCTAGCCCGGGCGCGCCTGTCGCTACGGATGGGCTGGT
CGGATACCCCCGCCCTGCTGGCGCTGGTTCCCCCTGCCCTGTCCGACGATCCGGTGCTGGTGCTGGACCGGATTCGCTGG
CTGCGCCGGTCCGGGCAGGCCGACCTCGCCGCCGCGCTGTGGGCCCAGTCGGGGCCGGCGGCAGAGCAGCGCGTGGCGGA
TCCCGCCTTCGCGGCCGCCATAAAGGCCGCCTTCTGGGCGGAACGCGACGCCCTGGCCCACGACATGATGCTGGCCGGCC
GGGACAAGGAAGCCCTGGCCCTGGCCACGGCTTCCGACGACATGGCGCCCACGGACCGTAACGCGGCGCGGTTCCTGTCG
GGCTGGATCCTGCTGCAGCACGATCATGACCCGCATGCCGCGCTGGCCCGGTTCCGCCCGCTGTGCGACGCGTCGTCGCT
GCTGACGCGCAGCCGGGGCCTGTACTGGACCGGCCGTGCCCTGTCGGACCTGGGCGACCGGGATGGTGCCCGCACCGCCT
GGGGGCAGGCTGCCGCCCTGCCCGGCACCTTCTATGGCCAGATGGCGGCATCCCGGCTGGCAGGGGACACGGCCAGCCCC
CTGCTGTTCCCCGAGCGATCGGGCGACCTGGTGCGGTCGCGGCTGGGCGCCATTCCGGGGCCGGTCTGGACCCCGGACGA
CAAGGCGCGTTTCGATGCCAGCGACCTGGTGCAGGCCGCCCGGCTGCTGGCCGCACGGCATGATTTGGGTCATGCGCGCG
ATTTCCTGCTGTTGCAGGATACGCGGACAGGCGGGACCGCCGGCCACGTCCTGGCCGCGACGCTGGCGCTGCAGCTCGGC
CTGCCGGATGTCGCGGTGTCGATCGCCCGCCGCGCGGGGCGCGAGGGCACGGCATTGCTGCCCTTGGGCTGGCCCGTCCC
GTTCTCACCAGCCTTGTCCTCGGTCCCTGGCGAGGGGCTGCCCCCCGGTTTCCTGATGGCCGTGATCCGGCAGGAAAGCG
GGTTCGATCCCGGCATCGTCAGCCCGGCCGGCGCCTATGGCCTGATGCAGCTTATGCCGGCGGCGGCGCGGGACGTGTCG
CGGCAGGCCCGGCTGGCGACCGGTCCGCTGACCGGCGCGCGCCTGACGGACCCGGACCTGAACATACGTATCGGCAGCGC
CTACCTGGTGCGGCTGATGCAGAAATTCGGCGGCGCGGTGCCCTATGTGCTGGCGGCTTACAATGCCGGGCCCCACCGGG
CGGACGAATGGCTGGCCCTGCTGGGCGATCCGGCACGAGGCACGCCCACGGCCGACGCCATGCTGGACTGGATCGAAAGC
ATTCCGTTCGCGGAAACCAGAAGCTATATCCAGCGCGTGGAAGAAAACATGGCCATCTACCAGGCGCTGGCCGCCTCCGC
CCAGGCGGAGGCCGCGCCATGA

Upstream 100 bases:

>100_bases
GGCGTCGCCGTCGAACTGATCGTCAAGGGGGGCGGCGAGGTCTGGCACTCCGTGTCATAATGGCGCACGATCGTTCCTGG
TAACCGAAGCATATCCGGTC

Downstream 100 bases:

>100_bases
CCCCGTCCCGGCTGATCGCCAGCTTCGGCGGGTGCGGGTTTTCGCCCTTCGCGCCCGGCACGGTCGGGTCTTTGGCCGCG
CTGGCGTGCGGGTTGGGGCT

Product: soluble lytic murein transglycosylase

Products: 1,6-Anhydrobond [C]

Alternate protein names: Exomuramidase; Peptidoglycan lytic exotransglycosylase; Slt70 [H]

Number of amino acids: Translated: 673; Mature: 673

Protein sequence:

>673_residues
MRRLLPFFPLPVLACLALLGGSARADSSGLPEPPRSVPSDAVVPPAVPDGTSDAVAARVDTYLRLLSPLGGDIDEYLSFL
DQTPTWPRRAVMLGRLQRLMAVAAPGAPGMAQACATLTLTYAPALAACARQPVPAPDLPARARHAWTEGVDQMEDEAAFL
AAFAPVLTPADQWHRFMRQELTGHLDAAQRQVARVDIGRQALARARLSLRMGWSDTPALLALVPPALSDDPVLVLDRIRW
LRRSGQADLAAALWAQSGPAAEQRVADPAFAAAIKAAFWAERDALAHDMMLAGRDKEALALATASDDMAPTDRNAARFLS
GWILLQHDHDPHAALARFRPLCDASSLLTRSRGLYWTGRALSDLGDRDGARTAWGQAAALPGTFYGQMAASRLAGDTASP
LLFPERSGDLVRSRLGAIPGPVWTPDDKARFDASDLVQAARLLAARHDLGHARDFLLLQDTRTGGTAGHVLAATLALQLG
LPDVAVSIARRAGREGTALLPLGWPVPFSPALSSVPGEGLPPGFLMAVIRQESGFDPGIVSPAGAYGLMQLMPAAARDVS
RQARLATGPLTGARLTDPDLNIRIGSAYLVRLMQKFGGAVPYVLAAYNAGPHRADEWLALLGDPARGTPTADAMLDWIES
IPFAETRSYIQRVEENMAIYQALAASAQAEAAP

Sequences:

>Translated_673_residues
MRRLLPFFPLPVLACLALLGGSARADSSGLPEPPRSVPSDAVVPPAVPDGTSDAVAARVDTYLRLLSPLGGDIDEYLSFL
DQTPTWPRRAVMLGRLQRLMAVAAPGAPGMAQACATLTLTYAPALAACARQPVPAPDLPARARHAWTEGVDQMEDEAAFL
AAFAPVLTPADQWHRFMRQELTGHLDAAQRQVARVDIGRQALARARLSLRMGWSDTPALLALVPPALSDDPVLVLDRIRW
LRRSGQADLAAALWAQSGPAAEQRVADPAFAAAIKAAFWAERDALAHDMMLAGRDKEALALATASDDMAPTDRNAARFLS
GWILLQHDHDPHAALARFRPLCDASSLLTRSRGLYWTGRALSDLGDRDGARTAWGQAAALPGTFYGQMAASRLAGDTASP
LLFPERSGDLVRSRLGAIPGPVWTPDDKARFDASDLVQAARLLAARHDLGHARDFLLLQDTRTGGTAGHVLAATLALQLG
LPDVAVSIARRAGREGTALLPLGWPVPFSPALSSVPGEGLPPGFLMAVIRQESGFDPGIVSPAGAYGLMQLMPAAARDVS
RQARLATGPLTGARLTDPDLNIRIGSAYLVRLMQKFGGAVPYVLAAYNAGPHRADEWLALLGDPARGTPTADAMLDWIES
IPFAETRSYIQRVEENMAIYQALAASAQAEAAP
>Mature_673_residues
MRRLLPFFPLPVLACLALLGGSARADSSGLPEPPRSVPSDAVVPPAVPDGTSDAVAARVDTYLRLLSPLGGDIDEYLSFL
DQTPTWPRRAVMLGRLQRLMAVAAPGAPGMAQACATLTLTYAPALAACARQPVPAPDLPARARHAWTEGVDQMEDEAAFL
AAFAPVLTPADQWHRFMRQELTGHLDAAQRQVARVDIGRQALARARLSLRMGWSDTPALLALVPPALSDDPVLVLDRIRW
LRRSGQADLAAALWAQSGPAAEQRVADPAFAAAIKAAFWAERDALAHDMMLAGRDKEALALATASDDMAPTDRNAARFLS
GWILLQHDHDPHAALARFRPLCDASSLLTRSRGLYWTGRALSDLGDRDGARTAWGQAAALPGTFYGQMAASRLAGDTASP
LLFPERSGDLVRSRLGAIPGPVWTPDDKARFDASDLVQAARLLAARHDLGHARDFLLLQDTRTGGTAGHVLAATLALQLG
LPDVAVSIARRAGREGTALLPLGWPVPFSPALSSVPGEGLPPGFLMAVIRQESGFDPGIVSPAGAYGLMQLMPAAARDVS
RQARLATGPLTGARLTDPDLNIRIGSAYLVRLMQKFGGAVPYVLAAYNAGPHRADEWLALLGDPARGTPTADAMLDWIES
IPFAETRSYIQRVEENMAIYQALAASAQAEAAP

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasm. Note=Tightly associated with the murein sacculus (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=137, Percent_Identity=42.3357664233577, Blast_Score=102, Evalue=7e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016026
- InterPro:   IPR008258
- InterPro:   IPR012289
- InterPro:   IPR008939
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 71602; Mature: 71602

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRLLPFFPLPVLACLALLGGSARADSSGLPEPPRSVPSDAVVPPAVPDGTSDAVAARVD
CCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
TYLRLLSPLGGDIDEYLSFLDQTPTWPRRAVMLGRLQRLMAVAAPGAPGMAQACATLTLT
HHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
YAPALAACARQPVPAPDLPARARHAWTEGVDQMEDEAAFLAAFAPVLTPADQWHRFMRQE
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
LTGHLDAAQRQVARVDIGRQALARARLSLRMGWSDTPALLALVPPALSDDPVLVLDRIRW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCHHHHHHHH
LRRSGQADLAAALWAQSGPAAEQRVADPAFAAAIKAAFWAERDALAHDMMLAGRDKEALA
HHHCCCHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
LATASDDMAPTDRNAARFLSGWILLQHDHDPHAALARFRPLCDASSLLTRSRGLYWTGRA
EEECCCCCCCCCCHHHHHHHCEEEEEECCCHHHHHHHHHHHCCHHHHHHHCCCCEEECCH
LSDLGDRDGARTAWGQAAALPGTFYGQMAASRLAGDTASPLLFPERSGDLVRSRLGAIPG
HHHCCCCCCCCCCCCCHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCC
PVWTPDDKARFDASDLVQAARLLAARHDLGHARDFLLLQDTRTGGTAGHVLAATLALQLG
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHCC
LPDVAVSIARRAGREGTALLPLGWPVPFSPALSSVPGEGLPPGFLMAVIRQESGFDPGIV
CHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCC
SPAGAYGLMQLMPAAARDVSRQARLATGPLTGARLTDPDLNIRIGSAYLVRLMQKFGGAV
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCEEEECHHHHHHHHHHHCCCC
PYVLAAYNAGPHRADEWLALLGDPARGTPTADAMLDWIESIPFAETRSYIQRVEENMAIY
CHHHEECCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
QALAASAQAEAAP
HHHHHHHHCCCCC
>Mature Secondary Structure
MRRLLPFFPLPVLACLALLGGSARADSSGLPEPPRSVPSDAVVPPAVPDGTSDAVAARVD
CCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
TYLRLLSPLGGDIDEYLSFLDQTPTWPRRAVMLGRLQRLMAVAAPGAPGMAQACATLTLT
HHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
YAPALAACARQPVPAPDLPARARHAWTEGVDQMEDEAAFLAAFAPVLTPADQWHRFMRQE
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
LTGHLDAAQRQVARVDIGRQALARARLSLRMGWSDTPALLALVPPALSDDPVLVLDRIRW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCHHHHHHHH
LRRSGQADLAAALWAQSGPAAEQRVADPAFAAAIKAAFWAERDALAHDMMLAGRDKEALA
HHHCCCHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
LATASDDMAPTDRNAARFLSGWILLQHDHDPHAALARFRPLCDASSLLTRSRGLYWTGRA
EEECCCCCCCCCCHHHHHHHCEEEEEECCCHHHHHHHHHHHCCHHHHHHHCCCCEEECCH
LSDLGDRDGARTAWGQAAALPGTFYGQMAASRLAGDTASPLLFPERSGDLVRSRLGAIPG
HHHCCCCCCCCCCCCCHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCC
PVWTPDDKARFDASDLVQAARLLAARHDLGHARDFLLLQDTRTGGTAGHVLAATLALQLG
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHCC
LPDVAVSIARRAGREGTALLPLGWPVPFSPALSSVPGEGLPPGFLMAVIRQESGFDPGIV
CHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCC
SPAGAYGLMQLMPAAARDVSRQARLATGPLTGARLTDPDLNIRIGSAYLVRLMQKFGGAV
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCEEEECHHHHHHHHHHHCCCC
PYVLAAYNAGPHRADEWLALLGDPARGTPTADAMLDWIESIPFAETRSYIQRVEENMAIY
CHHHEECCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
QALAASAQAEAAP
HHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]