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The map label for this gene is pyrG

Identifier: 162147714

GI number: 162147714

Start: 1963563

End: 1965194

Strand: Reverse

Name: pyrG

Synonym: GDI_1930

Alternate gene names: 162147714

Gene position: 1965194-1963563 (Counterclockwise)

Preceding gene: 162147715

Following gene: 162147713

Centisome position: 49.83

GC content: 64.46

Gene sequence:

>1632_bases
ATGACGCGGTTTGTATTCATCACTGGCGGCGTGGTCTCCTCACTCGGTAAAGGCATTGCCTCGGCAGCCCTCGCGGCCCT
GCTGCAGGCGCGCGGATACCGGGTCCGGCTACGCAAGCTCGATCCTTATCTCAACGTCGATCCGGGCACCATGAGCCCGT
ATCAACATGGCGAAGTCTTCGTAACCGACGATGGCGCGGAGACCGATCTGGACCTTGGACATTATGAACGCTTCACCGGC
GTCCATGCGACCAGGGCCGACAACGCCACAACCGGCCAGATCTATTCCGACGTGATCGCCCGCGAACGGCGCGGCGATTA
CCTGGGCGCGACCGTTCAGGTCATCCCCCACATCACGGACGCCATCAAGGAAGCCGTGGTCGCGGGGACCGAGGACCTGG
ATTTCGTGCTGGTGGAAATCGGCGGCACCGTGGGCGACATCGAAAGCCTGCCGTTCCTCGAGGCCATTCGCCAGTTGCGC
AACGACCTGGGCGCCGGGCAGACGATGTTCGTCCACCTGACCCTGCTGCCGTGGATTCCCTCGGCCGGCGAACTGAAGAC
CAAGCCGACGCAGCATTCCGTCAAGGAACTGCAGAATGTCGGTATCCAGGCCCAGATGCTGCTGTGCCGGTCCGACCGGC
CGATTCCCGACACCGAACGGCGCAAGATCGCCAATTTCTGCAATGTGCGGCCCGAGGCCGTGATCGCGGCGCTGGACGTC
GACACCATCTATGCCTGCCCGGTCTCGTACCACGCCGAAGGCATGGATACCGAGGTCCTGCGCCATTTCGGCCTGCCCCA
TGACCAGGAACCCGACCTGTCGGCATGGAACCGGGTGCTGGACGCGATGCGCCACCCCGAGGGCGAGGTGCGGATCGCCG
TGGTGGGCAAATACACGGCCCTGCTGGATGCCTATAAATCGCTGATCGAGGCGCTGCAGCATGGCGGCATCGCCAACCGG
GTCCGCGTCAAGCTGGACTGGGTCGAGGCCGAGATCTTCGAGAAGTCGGAAACGGCGATCGAGGCGCTGCGCGACGCGCA
CGCGATCCTGGTTCCCGGCGGTTTCGGCGAACGCGGGTCCGAAGGCAAGATCCAGGCCGTACGCTTCGCACGGGAACACA
ACATCCCGTTCCTGGGCATCTGTTTCGGGATGCAGATGGCTGTCATCGAATGCGCCCGCAACCTGGCCGGCCTGCCCGAT
GCGTCTTCGACCGAATTCGGCCCGACGGAGGAACCCCTGGTCGGCCTGATGACGGAATGGGCGCGGGGCAACGAATTGCT
GCGCCGGCGCGAGGGCGGCGAGATGGGCGGGACGATGCGCCTGGGCGCCTATGCCGCCAAGCTGGCCGAAGGCTCCCGCG
TGGCTGAAATCTACGGCAAGACCGAGATCCGCGAACGCCACCGCCACCGCTACGAGGTGAACGTGCATTACCGCGAGGTG
CTGGAGAAGGCCGGATTGCAGTTCTCGGGCATGTCGCCGGACGACATCCTGCCGGAAGTGGTGGAGTATCCGAACCATCC
CTGGTTCGTCGCGGTGCAGTACCATCCCGAACTGCTGTCCAAGCCGTTCGATCCGCATCCGCTTTTCTCGGGCTTCGTCG
GCGCGGCCGTGAAGAAGATGCGGCTGGTATGA

Upstream 100 bases:

>100_bases
CCCCGTCGCATGGCGGGGATGGTTTTTTATCGCCCCGATCGGACGGTCCGTCCGGTATGGACTTTTCACGGCGGGGCGAA
ACAGTGTAGGGAGGCCATCC

Downstream 100 bases:

>100_bases
CCCATCCGAAATCCGTCTCGCTCGGCAGCCTGACCGTCGGCAACGATCGTCCCTTCGTCCTGATCGCCGGGCCGTGCCAG
ATCGAATCCGAAGCCCATGC

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase

Number of amino acids: Translated: 543; Mature: 542

Protein sequence:

>543_residues
MTRFVFITGGVVSSLGKGIASAALAALLQARGYRVRLRKLDPYLNVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFTG
VHATRADNATTGQIYSDVIARERRGDYLGATVQVIPHITDAIKEAVVAGTEDLDFVLVEIGGTVGDIESLPFLEAIRQLR
NDLGAGQTMFVHLTLLPWIPSAGELKTKPTQHSVKELQNVGIQAQMLLCRSDRPIPDTERRKIANFCNVRPEAVIAALDV
DTIYACPVSYHAEGMDTEVLRHFGLPHDQEPDLSAWNRVLDAMRHPEGEVRIAVVGKYTALLDAYKSLIEALQHGGIANR
VRVKLDWVEAEIFEKSETAIEALRDAHAILVPGGFGERGSEGKIQAVRFAREHNIPFLGICFGMQMAVIECARNLAGLPD
ASSTEFGPTEEPLVGLMTEWARGNELLRRREGGEMGGTMRLGAYAAKLAEGSRVAEIYGKTEIRERHRHRYEVNVHYREV
LEKAGLQFSGMSPDDILPEVVEYPNHPWFVAVQYHPELLSKPFDPHPLFSGFVGAAVKKMRLV

Sequences:

>Translated_543_residues
MTRFVFITGGVVSSLGKGIASAALAALLQARGYRVRLRKLDPYLNVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFTG
VHATRADNATTGQIYSDVIARERRGDYLGATVQVIPHITDAIKEAVVAGTEDLDFVLVEIGGTVGDIESLPFLEAIRQLR
NDLGAGQTMFVHLTLLPWIPSAGELKTKPTQHSVKELQNVGIQAQMLLCRSDRPIPDTERRKIANFCNVRPEAVIAALDV
DTIYACPVSYHAEGMDTEVLRHFGLPHDQEPDLSAWNRVLDAMRHPEGEVRIAVVGKYTALLDAYKSLIEALQHGGIANR
VRVKLDWVEAEIFEKSETAIEALRDAHAILVPGGFGERGSEGKIQAVRFAREHNIPFLGICFGMQMAVIECARNLAGLPD
ASSTEFGPTEEPLVGLMTEWARGNELLRRREGGEMGGTMRLGAYAAKLAEGSRVAEIYGKTEIRERHRHRYEVNVHYREV
LEKAGLQFSGMSPDDILPEVVEYPNHPWFVAVQYHPELLSKPFDPHPLFSGFVGAAVKKMRLV
>Mature_542_residues
TRFVFITGGVVSSLGKGIASAALAALLQARGYRVRLRKLDPYLNVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFTGV
HATRADNATTGQIYSDVIARERRGDYLGATVQVIPHITDAIKEAVVAGTEDLDFVLVEIGGTVGDIESLPFLEAIRQLRN
DLGAGQTMFVHLTLLPWIPSAGELKTKPTQHSVKELQNVGIQAQMLLCRSDRPIPDTERRKIANFCNVRPEAVIAALDVD
TIYACPVSYHAEGMDTEVLRHFGLPHDQEPDLSAWNRVLDAMRHPEGEVRIAVVGKYTALLDAYKSLIEALQHGGIANRV
RVKLDWVEAEIFEKSETAIEALRDAHAILVPGGFGERGSEGKIQAVRFAREHNIPFLGICFGMQMAVIECARNLAGLPDA
SSTEFGPTEEPLVGLMTEWARGNELLRRREGGEMGGTMRLGAYAAKLAEGSRVAEIYGKTEIRERHRHRYEVNVHYREVL
EKAGLQFSGMSPDDILPEVVEYPNHPWFVAVQYHPELLSKPFDPHPLFSGFVGAAVKKMRLV

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI148491070, Length=560, Percent_Identity=43.3928571428571, Blast_Score=466, Evalue=1e-131,
Organism=Homo sapiens, GI28559085, Length=561, Percent_Identity=42.602495543672, Blast_Score=442, Evalue=1e-124,
Organism=Homo sapiens, GI28559083, Length=561, Percent_Identity=42.602495543672, Blast_Score=442, Evalue=1e-124,
Organism=Homo sapiens, GI221316689, Length=561, Percent_Identity=42.602495543672, Blast_Score=442, Evalue=1e-124,
Organism=Escherichia coli, GI1789142, Length=537, Percent_Identity=58.4729981378026, Blast_Score=606, Evalue=1e-174,
Organism=Caenorhabditis elegans, GI25148299, Length=609, Percent_Identity=36.2889983579639, Blast_Score=390, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6319432, Length=568, Percent_Identity=41.0211267605634, Blast_Score=421, Evalue=1e-118,
Organism=Saccharomyces cerevisiae, GI6322563, Length=570, Percent_Identity=40.8771929824561, Blast_Score=415, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24664469, Length=560, Percent_Identity=44.1071428571429, Blast_Score=463, Evalue=1e-130,
Organism=Drosophila melanogaster, GI21357815, Length=506, Percent_Identity=43.8735177865613, Blast_Score=401, Evalue=1e-112,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRG_GLUDA (A9HJ81)

Other databases:

- EMBL:   CP001189
- EMBL:   AM889285
- RefSeq:   YP_001602175.1
- RefSeq:   YP_002274567.1
- ProteinModelPortal:   A9HJ81
- SMR:   A9HJ81
- GeneID:   5790184
- GeneID:   6973544
- GenomeReviews:   AM889285_GR
- GenomeReviews:   CP001189_GR
- KEGG:   gdj:Gdia_0152
- HOGENOM:   HBG597806
- OMA:   TEWLRGN
- ProtClustDB:   PRK05380
- HAMAP:   MF_01227
- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991
- TIGRFAMs:   TIGR00337

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase

EC number: =6.3.4.2

Molecular weight: Translated: 60020; Mature: 59889

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 381-381 ACT_SITE 515-515 ACT_SITE 517-517

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRFVFITGGVVSSLGKGIASAALAALLQARGYRVRLRKLDPYLNVDPGTMSPYQHGEVF
CCEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCEEE
VTDDGAETDLDLGHYERFTGVHATRADNATTGQIYSDVIARERRGDYLGATVQVIPHITD
EECCCCCCCCCCHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
AIKEAVVAGTEDLDFVLVEIGGTVGDIESLPFLEAIRQLRNDLGAGQTMFVHLTLLPWIP
HHHHHHHCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEECCCC
SAGELKTKPTQHSVKELQNVGIQAQMLLCRSDRPIPDTERRKIANFCNVRPEAVIAALDV
CCCCCCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCHHHEEEEECC
DTIYACPVSYHAEGMDTEVLRHFGLPHDQEPDLSAWNRVLDAMRHPEGEVRIAVVGKYTA
CEEEECCCCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECHHHH
LLDAYKSLIEALQHGGIANRVRVKLDWVEAEIFEKSETAIEALRDAHAILVPGGFGERGS
HHHHHHHHHHHHHHCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCC
EGKIQAVRFAREHNIPFLGICFGMQMAVIECARNLAGLPDASSTEFGPTEEPLVGLMTEW
CCCCHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHH
ARGNELLRRREGGEMGGTMRLGAYAAKLAEGSRVAEIYGKTEIRERHRHRYEVNVHYREV
HHHHHHHHHHCCCCCCCEEEHHHHHHHHHCCCHHHHHHCHHHHHHHHCCEEEEEEHHHHH
LEKAGLQFSGMSPDDILPEVVEYPNHPWFVAVQYHPELLSKPFDPHPLFSGFVGAAVKKM
HHHCCCEECCCCCHHHHHHHHHCCCCCEEEEEEECHHHHCCCCCCCHHHHHHHHHHHHHH
RLV
CCC
>Mature Secondary Structure 
TRFVFITGGVVSSLGKGIASAALAALLQARGYRVRLRKLDPYLNVDPGTMSPYQHGEVF
CEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCEEE
VTDDGAETDLDLGHYERFTGVHATRADNATTGQIYSDVIARERRGDYLGATVQVIPHITD
EECCCCCCCCCCHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
AIKEAVVAGTEDLDFVLVEIGGTVGDIESLPFLEAIRQLRNDLGAGQTMFVHLTLLPWIP
HHHHHHHCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEECCCC
SAGELKTKPTQHSVKELQNVGIQAQMLLCRSDRPIPDTERRKIANFCNVRPEAVIAALDV
CCCCCCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCHHHEEEEECC
DTIYACPVSYHAEGMDTEVLRHFGLPHDQEPDLSAWNRVLDAMRHPEGEVRIAVVGKYTA
CEEEECCCCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECHHHH
LLDAYKSLIEALQHGGIANRVRVKLDWVEAEIFEKSETAIEALRDAHAILVPGGFGERGS
HHHHHHHHHHHHHHCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCC
EGKIQAVRFAREHNIPFLGICFGMQMAVIECARNLAGLPDASSTEFGPTEEPLVGLMTEW
CCCCHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHH
ARGNELLRRREGGEMGGTMRLGAYAAKLAEGSRVAEIYGKTEIRERHRHRYEVNVHYREV
HHHHHHHHHHCCCCCCCEEEHHHHHHHHHCCCHHHHHHCHHHHHHHHCCEEEEEEHHHHH
LEKAGLQFSGMSPDDILPEVVEYPNHPWFVAVQYHPELLSKPFDPHPLFSGFVGAAVKKM
HHHCCCEECCCCCHHHHHHHHHCCCCCEEEEEEECHHHHCCCCCCCHHHHHHHHHHHHHH
RLV
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA