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The map label for this gene is eno

Identifier: 162147711

GI number: 162147711

Start: 1960816

End: 1962096

Strand: Reverse

Name: eno

Synonym: GDI_1927

Alternate gene names: 162147711

Gene position: 1962096-1960816 (Counterclockwise)

Preceding gene: 162147713

Following gene: 162147710

Centisome position: 49.75

GC content: 66.04

Gene sequence:

>1281_bases
ATGAGTGCTATCGTCGATATCGTCGCGCGCGAAATCCTGGACAGCCGCGGCAATCCGACTGTCGAGGTCGATGTCGAACT
GGCGTCGGGTGCCAAGGGACGCGCCGCCGTTCCGTCGGGCGCATCCACCGGCGCGCATGAGGCCGTCGAGCTTCGCGACG
GCGACAAGTCCCGATTCGGCGGCAAGGGCGTCCTGAAGGCGGTCGAGCATGTCGAGACGGAGATCCTGGAGGCCCTGCAG
GGCGCCGAATCAATGGACCAGGTGGCGATCGACGAGGCGATGATCGACCTGGACGGCACGCCGAACAAGGCCCGCCTGGG
CGCCAACGCCATCCTGGCGGTATCGCTGGCGGTGGCCAAGGCCTCGGCCGAGGAACTGCAGATCCCGCTCTATCGCTATG
TCGGCGGCGTCTATGCCCGCACCCTGCCGGTGCCGATGATGAACATCGTCAATGGCGGCCAGCATGCCGACAACCCGATC
GACATCCAGGAATTCATGATCCAGCCGGTGGGCGCCCCCACCCTGGCCGACGCGGTGCGCGTGGGTTCGGAAATCTTCGC
CCAGTTGAAGAAGAACCTGTCGGCCGCCGGCCATAACACCAATGTCGGTGACGAGGGCGGCTTCGCCCCCGGCCTGAAAT
CCGCCGACGACGCACTGGGCTTCATCACCAAGGCGGTCGAGGCCGCCGGTTACCGCCCCGGCGACGATGTGACCTTCGCG
CTGGATTGCGCTGCGACCGAATTCTACCGTGACGGCCTCTATGTCATGGAAGGCGAAGGCAAGACCCTGGATTCCGCCGG
CATGGTGGCCTACCTGGCCGACCTGGCCGCGCGCTACCCGATCGTGTCGATCGAGGACGGCCTGGCGGAAGACGACTGGG
AAGGCTGGGCCGTGCTGACCGCGACGCTGGGCAAGACGGTGCAACTGGTGGGTGACGATCTGTTCGTGACCAATCCGGAC
CGCCTGCGCCGTGGCATCAAGGCCGGGGTGGCGAATTCGCTGCTGGTGAAGGTGAACCAGATCGGCACGCTGAGCGAGAC
GCTGGAAGCGGTCGAGACGGCGCAGCGCGCGGGCTATACCGCCGTGATGAGCCACCGTTCGGGCGAAACCGAGGATTCGA
CCATCGCCGACCTGGCCGTCGCGACCAATTGCGGGCAGATCAAGACCGGCTCGCTGTCGCGCTCCGACCGCACGGCGAAA
TACAACCAGCTGATCCGGATCGAGAACGAACTGGCCACTGCGGCCCGTTACGCTGGCCGGACGATCCTCAAGACGGCCTG
A

Upstream 100 bases:

>100_bases
CCGAAGGGGAGGGCCCGGTCGCGGTCGCACGTCCCGGTCGAACCTGCAACGGGCCCAATATCCACAAACCCCAATATCCA
CAAACCGAGGAAGCAGAGTC

Downstream 100 bases:

>100_bases
TCGCGCGGAAAACGTACCGATCCGGCGGAAAGGGAGGGAAAAACTGTTCCCTCCCGCCACCGGATCGGGAATAGTTCGCG
ATGGAATTCGCTGAACGCGA

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MSAIVDIVAREILDSRGNPTVEVDVELASGAKGRAAVPSGASTGAHEAVELRDGDKSRFGGKGVLKAVEHVETEILEALQ
GAESMDQVAIDEAMIDLDGTPNKARLGANAILAVSLAVAKASAEELQIPLYRYVGGVYARTLPVPMMNIVNGGQHADNPI
DIQEFMIQPVGAPTLADAVRVGSEIFAQLKKNLSAAGHNTNVGDEGGFAPGLKSADDALGFITKAVEAAGYRPGDDVTFA
LDCAATEFYRDGLYVMEGEGKTLDSAGMVAYLADLAARYPIVSIEDGLAEDDWEGWAVLTATLGKTVQLVGDDLFVTNPD
RLRRGIKAGVANSLLVKVNQIGTLSETLEAVETAQRAGYTAVMSHRSGETEDSTIADLAVATNCGQIKTGSLSRSDRTAK
YNQLIRIENELATAARYAGRTILKTA

Sequences:

>Translated_426_residues
MSAIVDIVAREILDSRGNPTVEVDVELASGAKGRAAVPSGASTGAHEAVELRDGDKSRFGGKGVLKAVEHVETEILEALQ
GAESMDQVAIDEAMIDLDGTPNKARLGANAILAVSLAVAKASAEELQIPLYRYVGGVYARTLPVPMMNIVNGGQHADNPI
DIQEFMIQPVGAPTLADAVRVGSEIFAQLKKNLSAAGHNTNVGDEGGFAPGLKSADDALGFITKAVEAAGYRPGDDVTFA
LDCAATEFYRDGLYVMEGEGKTLDSAGMVAYLADLAARYPIVSIEDGLAEDDWEGWAVLTATLGKTVQLVGDDLFVTNPD
RLRRGIKAGVANSLLVKVNQIGTLSETLEAVETAQRAGYTAVMSHRSGETEDSTIADLAVATNCGQIKTGSLSRSDRTAK
YNQLIRIENELATAARYAGRTILKTA
>Mature_425_residues
SAIVDIVAREILDSRGNPTVEVDVELASGAKGRAAVPSGASTGAHEAVELRDGDKSRFGGKGVLKAVEHVETEILEALQG
AESMDQVAIDEAMIDLDGTPNKARLGANAILAVSLAVAKASAEELQIPLYRYVGGVYARTLPVPMMNIVNGGQHADNPID
IQEFMIQPVGAPTLADAVRVGSEIFAQLKKNLSAAGHNTNVGDEGGFAPGLKSADDALGFITKAVEAAGYRPGDDVTFAL
DCAATEFYRDGLYVMEGEGKTLDSAGMVAYLADLAARYPIVSIEDGLAEDDWEGWAVLTATLGKTVQLVGDDLFVTNPDR
LRRGIKAGVANSLLVKVNQIGTLSETLEAVETAQRAGYTAVMSHRSGETEDSTIADLAVATNCGQIKTGSLSRSDRTAKY
NQLIRIENELATAARYAGRTILKTA

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI5803011, Length=432, Percent_Identity=50.6944444444444, Blast_Score=410, Evalue=1e-114,
Organism=Homo sapiens, GI301897477, Length=432, Percent_Identity=49.3055555555556, Blast_Score=397, Evalue=1e-111,
Organism=Homo sapiens, GI301897469, Length=432, Percent_Identity=49.3055555555556, Blast_Score=397, Evalue=1e-111,
Organism=Homo sapiens, GI4503571, Length=433, Percent_Identity=49.6535796766744, Blast_Score=394, Evalue=1e-109,
Organism=Homo sapiens, GI301897479, Length=430, Percent_Identity=45.1162790697674, Blast_Score=346, Evalue=3e-95,
Organism=Homo sapiens, GI169201331, Length=340, Percent_Identity=26.7647058823529, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI169201757, Length=340, Percent_Identity=26.7647058823529, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI239744207, Length=340, Percent_Identity=26.7647058823529, Blast_Score=100, Evalue=4e-21,
Organism=Escherichia coli, GI1789141, Length=431, Percent_Identity=61.9489559164733, Blast_Score=514, Evalue=1e-147,
Organism=Caenorhabditis elegans, GI17536383, Length=432, Percent_Identity=48.1481481481481, Blast_Score=380, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI71995829, Length=432, Percent_Identity=48.1481481481481, Blast_Score=380, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI32563855, Length=192, Percent_Identity=44.2708333333333, Blast_Score=172, Evalue=4e-43,
Organism=Saccharomyces cerevisiae, GI6321693, Length=437, Percent_Identity=48.7414187643021, Blast_Score=383, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6323985, Length=436, Percent_Identity=49.0825688073394, Blast_Score=378, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6324974, Length=436, Percent_Identity=49.0825688073394, Blast_Score=378, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6324969, Length=436, Percent_Identity=49.0825688073394, Blast_Score=378, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6321968, Length=437, Percent_Identity=48.2837528604119, Blast_Score=362, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580918, Length=431, Percent_Identity=48.2598607888631, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24580916, Length=431, Percent_Identity=48.2598607888631, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24580920, Length=431, Percent_Identity=48.2598607888631, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24580914, Length=431, Percent_Identity=48.2598607888631, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI281360527, Length=431, Percent_Identity=48.2598607888631, Blast_Score=368, Evalue=1e-102,
Organism=Drosophila melanogaster, GI17137654, Length=431, Percent_Identity=48.2598607888631, Blast_Score=368, Evalue=1e-102,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_GLUDA (A9HJ75)

Other databases:

- EMBL:   AM889285
- EMBL:   CP001189
- RefSeq:   YP_001602172.1
- RefSeq:   YP_002274564.1
- ProteinModelPortal:   A9HJ75
- SMR:   A9HJ75
- GeneID:   5790181
- GeneID:   6973541
- GenomeReviews:   AM889285_GR
- GenomeReviews:   CP001189_GR
- KEGG:   gdj:Gdia_0149
- HOGENOM:   HBG726599
- OMA:   DIAVGTN
- ProtClustDB:   PRK00077
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 44809; Mature: 44678

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 205-205 ACT_SITE 337-337 BINDING 155-155 BINDING 164-164 BINDING 285-285 BINDING 312-312 BINDING 337-337 BINDING 388-388

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAIVDIVAREILDSRGNPTVEVDVELASGAKGRAAVPSGASTGAHEAVELRDGDKSRFG
CHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCC
GKGVLKAVEHVETEILEALQGAESMDQVAIDEAMIDLDGTPNKARLGANAILAVSLAVAK
CHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHEECCCCCCHHHHCCCHHHHHHHHHHH
ASAEELQIPLYRYVGGVYARTLPVPMMNIVNGGQHADNPIDIQEFMIQPVGAPTLADAVR
CCHHHHCCHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCHHHHHHCCCCCCHHHHHHH
VGSEIFAQLKKNLSAAGHNTNVGDEGGFAPGLKSADDALGFITKAVEAAGYRPGDDVTFA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEE
LDCAATEFYRDGLYVMEGEGKTLDSAGMVAYLADLAARYPIVSIEDGLAEDDWEGWAVLT
EEHHHHHHHHCCEEEECCCCCCCCCCHHHHHHHHHHHHCCEEEECCCCCCCCCCCEEEEE
ATLGKTVQLVGDDLFVTNPDRLRRGIKAGVANSLLVKVNQIGTLSETLEAVETAQRAGYT
EHHCCEEEEECCCEEEECHHHHHHHHHHCCCHHHEEEEHHCCCHHHHHHHHHHHHHCCCC
AVMSHRSGETEDSTIADLAVATNCGQIKTGSLSRSDRTAKYNQLIRIENELATAARYAGR
EEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
TILKTA
HHCCCC
>Mature Secondary Structure 
SAIVDIVAREILDSRGNPTVEVDVELASGAKGRAAVPSGASTGAHEAVELRDGDKSRFG
HHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCC
GKGVLKAVEHVETEILEALQGAESMDQVAIDEAMIDLDGTPNKARLGANAILAVSLAVAK
CHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHEECCCCCCHHHHCCCHHHHHHHHHHH
ASAEELQIPLYRYVGGVYARTLPVPMMNIVNGGQHADNPIDIQEFMIQPVGAPTLADAVR
CCHHHHCCHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCHHHHHHCCCCCCHHHHHHH
VGSEIFAQLKKNLSAAGHNTNVGDEGGFAPGLKSADDALGFITKAVEAAGYRPGDDVTFA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEE
LDCAATEFYRDGLYVMEGEGKTLDSAGMVAYLADLAARYPIVSIEDGLAEDDWEGWAVLT
EEHHHHHHHHCCEEEECCCCCCCCCCHHHHHHHHHHHHCCEEEECCCCCCCCCCCEEEEE
ATLGKTVQLVGDDLFVTNPDRLRRGIKAGVANSLLVKVNQIGTLSETLEAVETAQRAGYT
EHHCCEEEEECCCEEEECHHHHHHHHHHCCCHHHEEEEHHCCCHHHHHHHHHHHHHCCCC
AVMSHRSGETEDSTIADLAVATNCGQIKTGSLSRSDRTAKYNQLIRIENELATAARYAGR
EEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
TILKTA
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA