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The map label for this gene is lipB

Identifier: 162147681

GI number: 162147681

Start: 1933479

End: 1934177

Strand: Reverse

Name: lipB

Synonym: GDI_1897

Alternate gene names: 162147681

Gene position: 1934177-1933479 (Counterclockwise)

Preceding gene: 162147686

Following gene: 162147678

Centisome position: 49.04

GC content: 68.38

Gene sequence:

>699_bases
ATGCATGATCGACGGGCAATGACCGAAAAGACGATTCTCTGGAATTCCAGCCCCGGCCTGGTTCCCTATCCCGACGCGAT
CGCCGGGATGGAGCATCAGGTGGCGGGAATCCGCGACGGCAGCGCCCCGGAACGGGTCTGGCTGCTGGAACACCCCCCCA
CCTTCACCGCCGGAACCTCCGCCCGGGACGAGGATTTGTTCAATCCGCATCATTTCCCGACCTATGCGGCGGGCCGCGGC
GGGCAATGGACCTATCACGGGCCGGGACAGCGGGTGGCCTACGTGATGCTGGACCTGACCGCCCCGCACGGCGTGGTGCC
GGCCCGGGACCTGCGGGCCTATGTCCATACGCTGGAAGAATGGCTGATCCGTACCCTGCGCCGCTTCGACGTGACAGGCG
AACGCCGCGCGGACCGGATCGGCGTGTGGGTCGTGGATGCGCGCACCGGGGCGGAAAACAAGATCGCGGCCCTGGGGGTG
CGGGTTTCGCGCTGGACCTGCTGGCACGGCGTGGCGCTGAACGTGGCGCCGAACCTGGACGATTTCGGCGGCATCGTCCC
CTGCGGGATTCGCGAACACGGCGTGACCAGCCTGCATGCGCTGGGCCACAAGGTGAGCCTGGGCGACGTCGACGAGGCCC
TGCGCGCCACGTGGCAGGACCTGTTCGGTAGCGTGCCGACCCCGATCGGCACCGCCTGA

Upstream 100 bases:

>100_bases
CACAAGCACCTCAAGCTTGCGCGTCTACCATTCCGCCACGACCGCACCGTGACATCGGACATCGCCTGAGCGATACCGTG
CGACGGAGGCCGTATAAACG

Downstream 100 bases:

>100_bases
CGCTACAACTCCGCCGTCAGCGAGAAGAAATCCGCCGGAAATGCGGTACCGGTGCGCTGGTCGGTCAAGATGCAGGCGCG
TGCGGTTGTGGCGTTCGTCC

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase

Number of amino acids: Translated: 232; Mature: 232

Protein sequence:

>232_residues
MHDRRAMTEKTILWNSSPGLVPYPDAIAGMEHQVAGIRDGSAPERVWLLEHPPTFTAGTSARDEDLFNPHHFPTYAAGRG
GQWTYHGPGQRVAYVMLDLTAPHGVVPARDLRAYVHTLEEWLIRTLRRFDVTGERRADRIGVWVVDARTGAENKIAALGV
RVSRWTCWHGVALNVAPNLDDFGGIVPCGIREHGVTSLHALGHKVSLGDVDEALRATWQDLFGSVPTPIGTA

Sequences:

>Translated_232_residues
MHDRRAMTEKTILWNSSPGLVPYPDAIAGMEHQVAGIRDGSAPERVWLLEHPPTFTAGTSARDEDLFNPHHFPTYAAGRG
GQWTYHGPGQRVAYVMLDLTAPHGVVPARDLRAYVHTLEEWLIRTLRRFDVTGERRADRIGVWVVDARTGAENKIAALGV
RVSRWTCWHGVALNVAPNLDDFGGIVPCGIREHGVTSLHALGHKVSLGDVDEALRATWQDLFGSVPTPIGTA
>Mature_232_residues
MHDRRAMTEKTILWNSSPGLVPYPDAIAGMEHQVAGIRDGSAPERVWLLEHPPTFTAGTSARDEDLFNPHHFPTYAAGRG
GQWTYHGPGQRVAYVMLDLTAPHGVVPARDLRAYVHTLEEWLIRTLRRFDVTGERRADRIGVWVVDARTGAENKIAALGV
RVSRWTCWHGVALNVAPNLDDFGGIVPCGIREHGVTSLHALGHKVSLGDVDEALRATWQDLFGSVPTPIGTA

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family

Homologues:

Organism=Escherichia coli, GI87081767, Length=173, Percent_Identity=39.8843930635838, Blast_Score=119, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6323268, Length=179, Percent_Identity=29.0502793296089, Blast_Score=73, Evalue=4e-14,
Organism=Drosophila melanogaster, GI21355483, Length=156, Percent_Identity=38.4615384615385, Blast_Score=93, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPB_GLUDA (A9HJ12)

Other databases:

- EMBL:   AM889285
- EMBL:   CP001189
- RefSeq:   YP_001602142.1
- RefSeq:   YP_002274535.1
- ProteinModelPortal:   A9HJ12
- SMR:   A9HJ12
- GeneID:   5790150
- GeneID:   6973511
- GenomeReviews:   AM889285_GR
- GenomeReviews:   CP001189_GR
- KEGG:   gdj:Gdia_0120
- HOGENOM:   HBG716160
- OMA:   DLKRRRP
- ProtClustDB:   PRK14341
- GO:   GO:0005737
- HAMAP:   MF_00013
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605
- PIRSF:   PIRSF016262
- TIGRFAMs:   TIGR00214

Pfam domain/function: PF03099 BPL_LipA_LipB

EC number: =2.3.1.181

Molecular weight: Translated: 25519; Mature: 25519

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: PS01313 LIPB

Important sites: ACT_SITE 188-188

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHDRRAMTEKTILWNSSPGLVPYPDAIAGMEHQVAGIRDGSAPERVWLLEHPPTFTAGTS
CCCCHHCCCEEEEECCCCCCCCCCHHHHCCCHHCCCCCCCCCCCEEEEEECCCCCCCCCC
ARDEDLFNPHHFPTYAAGRGGQWTYHGPGQRVAYVMLDLTAPHGVVPARDLRAYVHTLEE
CCCCCCCCCCCCCCCCCCCCCCEEEECCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHH
WLIRTLRRFDVTGERRADRIGVWVVDARTGAENKIAALGVRVSRWTCWHGVALNVAPNLD
HHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCEEEEEEEEEEEEEECCEEEEECCCCH
DFGGIVPCGIREHGVTSLHALGHKVSLGDVDEALRATWQDLFGSVPTPIGTA
HHCCEECCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MHDRRAMTEKTILWNSSPGLVPYPDAIAGMEHQVAGIRDGSAPERVWLLEHPPTFTAGTS
CCCCHHCCCEEEEECCCCCCCCCCHHHHCCCHHCCCCCCCCCCCEEEEEECCCCCCCCCC
ARDEDLFNPHHFPTYAAGRGGQWTYHGPGQRVAYVMLDLTAPHGVVPARDLRAYVHTLEE
CCCCCCCCCCCCCCCCCCCCCCEEEECCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHH
WLIRTLRRFDVTGERRADRIGVWVVDARTGAENKIAALGVRVSRWTCWHGVALNVAPNLD
HHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCEEEEEEEEEEEEEECCEEEEECCCCH
DFGGIVPCGIREHGVTSLHALGHKVSLGDVDEALRATWQDLFGSVPTPIGTA
HHCCEECCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA