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The map label for this gene is rrmJ [H]

Identifier: 162147649

GI number: 162147649

Start: 1902697

End: 1903491

Strand: Reverse

Name: rrmJ [H]

Synonym: GDI_1865

Alternate gene names: 162147649

Gene position: 1903491-1902697 (Counterclockwise)

Preceding gene: 162147650

Following gene: 162147647

Centisome position: 48.26

GC content: 70.57

Gene sequence:

>795_bases
ATGAAGCGTCGCCCCCCCGCCGGTGGCGGCAAGAAGCGGGTTCCCGGCAAGGCACTGACCAGCAGCACGACCCCTGGCGG
CGCCAGCGCCCAGGCGGATTCCGGTGCCGCGCAATCGACCCGCAACCAGGCCGTTTCGCTGCGCACGGCACGCGGCCGCA
CGACCGCGCAGCAGCGGTGGCTCAACCGCCAGTTGAACGATCCGTATGTGCAGGCGGCCCGCAAGCAGGGCTGGCGGTCC
CGCGCCGCGTTCAAGCTGATCGAACTGGACGACCGGTTCCACCTGATCCGCCCCGGCATGCGCGTGATCGACCTGGGCGC
CGCCCCCGGCGGCTGGACCCAGGTCGCGGTGAAGCGCGGCGCGGCTTATGTGGTCGGCGTCGATCTGCTGCCGGTCGATC
CGGTGGCCGGCGCCACGATCATTGAGGGCGATTTCAACGACCCCGACATGCCGGCCCGGCTGATCGACCTGCTGGGCGGT
CCGGCCGACCTGGTCCTGTCGGACATGGCGCCCAACACGACCGGCCACGCCCCGACCGACCATCTGCGCATCATCGGGCT
GGCGGAACTGGCGCTGGATTTCGCGGTGAAGGTCCTGGCCCCCGGCGGCGGGTTCGTCGCCAAGGTCTTCCAGGGCGGGT
CGGAGAAGCAGATGCTCGCCCCGATGAAACAGGCCTTCGCCACCGTCCGCCACGCCAAGCCCCCGGCCAGCCGGAAGGAA
TCAAGTGAACTCTACGTGGTCGCCACCGGCTTCCGCCCCGAGCGGATCAGCCAGGACGACGTGGAGGCACCCTAG

Upstream 100 bases:

>100_bases
TGCGCATGATGCACGATTCGACCGCGATGGCTCTTCCCTCGCTGCCTTCCTCCCTTCCCTTCGCCCCGTCCTCGCTATCC
TCGGTGGGCGTGTCGCATCC

Downstream 100 bases:

>100_bases
CCCCGGCACGCCCAGGCGCGCCTCAGATTACACCCCGGTGTTGGTCCGGCGTGGGTCTGCGAGCACCGGAGCGGAGCGGC
CTGATGGGCCGTGAGCACCG

Product: ribosomal RNA large subunit methyltransferase J

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MKRRPPAGGGKKRVPGKALTSSTTPGGASAQADSGAAQSTRNQAVSLRTARGRTTAQQRWLNRQLNDPYVQAARKQGWRS
RAAFKLIELDDRFHLIRPGMRVIDLGAAPGGWTQVAVKRGAAYVVGVDLLPVDPVAGATIIEGDFNDPDMPARLIDLLGG
PADLVLSDMAPNTTGHAPTDHLRIIGLAELALDFAVKVLAPGGGFVAKVFQGGSEKQMLAPMKQAFATVRHAKPPASRKE
SSELYVVATGFRPERISQDDVEAP

Sequences:

>Translated_264_residues
MKRRPPAGGGKKRVPGKALTSSTTPGGASAQADSGAAQSTRNQAVSLRTARGRTTAQQRWLNRQLNDPYVQAARKQGWRS
RAAFKLIELDDRFHLIRPGMRVIDLGAAPGGWTQVAVKRGAAYVVGVDLLPVDPVAGATIIEGDFNDPDMPARLIDLLGG
PADLVLSDMAPNTTGHAPTDHLRIIGLAELALDFAVKVLAPGGGFVAKVFQGGSEKQMLAPMKQAFATVRHAKPPASRKE
SSELYVVATGFRPERISQDDVEAP
>Mature_264_residues
MKRRPPAGGGKKRVPGKALTSSTTPGGASAQADSGAAQSTRNQAVSLRTARGRTTAQQRWLNRQLNDPYVQAARKQGWRS
RAAFKLIELDDRFHLIRPGMRVIDLGAAPGGWTQVAVKRGAAYVVGVDLLPVDPVAGATIIEGDFNDPDMPARLIDLLGG
PADLVLSDMAPNTTGHAPTDHLRIIGLAELALDFAVKVLAPGGGFVAKVFQGGSEKQMLAPMKQAFATVRHAKPPASRKE
SSELYVVATGFRPERISQDDVEAP

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit [H]

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family [H]

Homologues:

Organism=Homo sapiens, GI7019377, Length=214, Percent_Identity=43.4579439252336, Blast_Score=185, Evalue=4e-47,
Organism=Homo sapiens, GI29029591, Length=207, Percent_Identity=38.6473429951691, Blast_Score=139, Evalue=2e-33,
Organism=Homo sapiens, GI29029589, Length=207, Percent_Identity=38.6473429951691, Blast_Score=139, Evalue=2e-33,
Organism=Homo sapiens, GI7110661, Length=207, Percent_Identity=38.6473429951691, Blast_Score=139, Evalue=3e-33,
Organism=Homo sapiens, GI194097365, Length=188, Percent_Identity=38.2978723404255, Blast_Score=128, Evalue=6e-30,
Organism=Escherichia coli, GI1789569, Length=208, Percent_Identity=44.7115384615385, Blast_Score=180, Evalue=7e-47,
Organism=Caenorhabditis elegans, GI17553474, Length=202, Percent_Identity=37.6237623762376, Blast_Score=146, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17554650, Length=198, Percent_Identity=35.3535353535354, Blast_Score=134, Evalue=6e-32,
Organism=Caenorhabditis elegans, GI71987561, Length=200, Percent_Identity=36, Blast_Score=128, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI71987550, Length=200, Percent_Identity=36, Blast_Score=128, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17553860, Length=203, Percent_Identity=35.4679802955665, Blast_Score=118, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6319535, Length=200, Percent_Identity=36.5, Blast_Score=128, Evalue=9e-31,
Organism=Saccharomyces cerevisiae, GI6319796, Length=195, Percent_Identity=34.8717948717949, Blast_Score=114, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6321302, Length=100, Percent_Identity=46, Blast_Score=102, Evalue=9e-23,
Organism=Drosophila melanogaster, GI21356387, Length=217, Percent_Identity=41.4746543778802, Blast_Score=156, Evalue=2e-38,
Organism=Drosophila melanogaster, GI18859957, Length=188, Percent_Identity=40.4255319148936, Blast_Score=122, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24648639, Length=211, Percent_Identity=33.6492890995261, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24647580, Length=213, Percent_Identity=33.3333333333333, Blast_Score=117, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015507
- InterPro:   IPR002877 [H]

Pfam domain/function: PF01728 FtsJ [H]

EC number: =2.1.1.166 [H]

Molecular weight: Translated: 28058; Mature: 28058

Theoretical pI: Translated: 10.75; Mature: 10.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRRPPAGGGKKRVPGKALTSSTTPGGASAQADSGAAQSTRNQAVSLRTARGRTTAQQRW
CCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCHHHHHHHEEEEEECCCCHHHHHHH
LNRQLNDPYVQAARKQGWRSRAAFKLIELDDRFHLIRPGMRVIDLGAAPGGWTQVAVKRG
HHHCCCCHHHHHHHHHCHHHHHHEEEEEECCCEEEECCCCEEEEECCCCCCHHHHHHHCC
AAYVVGVDLLPVDPVAGATIIEGDFNDPDMPARLIDLLGGPADLVLSDMAPNTTGHAPTD
CEEEEEEEEECCCCCCCCEEEECCCCCCCHHHHHHHHHCCCHHHEEECCCCCCCCCCCCC
HLRIIGLAELALDFAVKVLAPGGGFVAKVFQGGSEKQMLAPMKQAFATVRHAKPPASRKE
CEEEEHHHHHHHHHHHHEECCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
SSELYVVATGFRPERISQDDVEAP
CCCEEEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MKRRPPAGGGKKRVPGKALTSSTTPGGASAQADSGAAQSTRNQAVSLRTARGRTTAQQRW
CCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCHHHHHHHEEEEEECCCCHHHHHHH
LNRQLNDPYVQAARKQGWRSRAAFKLIELDDRFHLIRPGMRVIDLGAAPGGWTQVAVKRG
HHHCCCCHHHHHHHHHCHHHHHHEEEEEECCCEEEECCCCEEEEECCCCCCHHHHHHHCC
AAYVVGVDLLPVDPVAGATIIEGDFNDPDMPARLIDLLGGPADLVLSDMAPNTTGHAPTD
CEEEEEEEEECCCCCCCCEEEECCCCCCCHHHHHHHHHCCCHHHEEECCCCCCCCCCCCC
HLRIIGLAELALDFAVKVLAPGGGFVAKVFQGGSEKQMLAPMKQAFATVRHAKPPASRKE
CEEEEHHHHHHHHHHHHEECCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
SSELYVVATGFRPERISQDDVEAP
CCCEEEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA