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The map label for this gene is glcK [H]

Identifier: 162147648

GI number: 162147648

Start: 1901639

End: 1902556

Strand: Direct

Name: glcK [H]

Synonym: GDI_1864

Alternate gene names: 162147648

Gene position: 1901639-1902556 (Clockwise)

Preceding gene: 162147637

Following gene: 162147652

Centisome position: 48.21

GC content: 67.86

Gene sequence:

>918_bases
ATGACCGACTATCGGATCGGGATCGATTTCGGCGGGACCAAGATCGAGATTGCAGCTCTGGCCCGTAAGGACGGGCAGGA
ACTCGTCCGGCGGCGCATCGTCAACCCGGGCTATTATGACGGCGCGATCACCGCCATCCGCGACCTCGTCGCGGGCGTGG
ACACGGAACTGGGCGGCCAGGGGACCGTCGGGATCGGCATTCCGGGTTCGATCAGCCCAGACACCGGCGTCATCAAGAAC
GCCAACGCGACGTGGCTGAACAACCAGCCGTTCGGCCGCGACCTGACCGCCGCCGTGGGGCGCGAGGTCCGGGTCGAGAA
CGACGCCAACTGCTTCGCCCTGTCCGAGGCCGTGGACGGCGCGGGCGCGGGATTCGGCGTGGTCCTTGGCGTGATCATCG
GCACCGGCATGGGTGCCGGCATCATCGTGAACGGCAAGCTGCTGATCGGCGCCCATCACATTGCCGGGGAATGGGGACAC
GTGCCGCTGCCCTGGCCGCGAATCGAGGAAATGCCGCTGCCCAAATGCTTCTGCGGCAACGAGGGCTGCCTGGAACGCTA
TCTGAGCGGGTCGGCCCTGGCCCAGGACTGGAAGGGACCGGGCAACCGCAGCACCGCCGGGATCGAAAGCGCGGCCGAGG
ACGGGGACCAGACGGCGATCGGGGCGCTGGACCGCTATATGGACCGCATGGCGCGGGCCTGCGCCATGGCGATCAACTTC
CTCGACCCCGACGTCATCGTGCTGGGGGGCGGCGTATCCAACCTGCAATCGATCTATGACCGCGTGCCGCTGCTGATGCC
GCGCTACGTCATCACGCCGCGCTGCAATACGCCGATCGTGCGCAACCGCCACGGCGACAGCTCCGGCGTGCGAGGCGCGG
CCTGGCTATGGTCGGGAGACGAGGGTGTCGGCCCGTAA

Upstream 100 bases:

>100_bases
AGGGTGGAAACGCCCGCTTACCGGCTGTCTTTACGACATGGACCGACACACCATAAAAGAATCAGCCTCCATAGAAAATA
TCACGGATTACGGGATGGAC

Downstream 100 bases:

>100_bases
ACGCGCCCTGGTGGCGATCCGACGCGGATTTGCTGCGCTCCGGTGCTCACGGCCCATCAGGCCGCTCCGCTCCGGTGCTC
GCAGACCCACGCCGGACCAA

Product: glucokinase protein

Products: NA

Alternate protein names: D-fructose kinase; Manno(fructo)kinase [H]

Number of amino acids: Translated: 305; Mature: 304

Protein sequence:

>305_residues
MTDYRIGIDFGGTKIEIAALARKDGQELVRRRIVNPGYYDGAITAIRDLVAGVDTELGGQGTVGIGIPGSISPDTGVIKN
ANATWLNNQPFGRDLTAAVGREVRVENDANCFALSEAVDGAGAGFGVVLGVIIGTGMGAGIIVNGKLLIGAHHIAGEWGH
VPLPWPRIEEMPLPKCFCGNEGCLERYLSGSALAQDWKGPGNRSTAGIESAAEDGDQTAIGALDRYMDRMARACAMAINF
LDPDVIVLGGGVSNLQSIYDRVPLLMPRYVITPRCNTPIVRNRHGDSSGVRGAAWLWSGDEGVGP

Sequences:

>Translated_305_residues
MTDYRIGIDFGGTKIEIAALARKDGQELVRRRIVNPGYYDGAITAIRDLVAGVDTELGGQGTVGIGIPGSISPDTGVIKN
ANATWLNNQPFGRDLTAAVGREVRVENDANCFALSEAVDGAGAGFGVVLGVIIGTGMGAGIIVNGKLLIGAHHIAGEWGH
VPLPWPRIEEMPLPKCFCGNEGCLERYLSGSALAQDWKGPGNRSTAGIESAAEDGDQTAIGALDRYMDRMARACAMAINF
LDPDVIVLGGGVSNLQSIYDRVPLLMPRYVITPRCNTPIVRNRHGDSSGVRGAAWLWSGDEGVGP
>Mature_304_residues
TDYRIGIDFGGTKIEIAALARKDGQELVRRRIVNPGYYDGAITAIRDLVAGVDTELGGQGTVGIGIPGSISPDTGVIKNA
NATWLNNQPFGRDLTAAVGREVRVENDANCFALSEAVDGAGAGFGVVLGVIIGTGMGAGIIVNGKLLIGAHHIAGEWGHV
PLPWPRIEEMPLPKCFCGNEGCLERYLSGSALAQDWKGPGNRSTAGIESAAEDGDQTAIGALDRYMDRMARACAMAINFL
DPDVIVLGGGVSNLQSIYDRVPLLMPRYVITPRCNTPIVRNRHGDSSGVRGAAWLWSGDEGVGP

Specific function: Catalyzes the phosphorylation of fructose to fructose-6- P. Has also low level glucokinase activity in vitro. Is not able to phosphorylate D-ribose, D-mannitol, D-sorbitol, inositol, and L-threonine [H]

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family [H]

Homologues:

Organism=Homo sapiens, GI298566325, Length=225, Percent_Identity=29.7777777777778, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI190014632, Length=225, Percent_Identity=29.7777777777778, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI4885285, Length=225, Percent_Identity=29.7777777777778, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI298566315, Length=225, Percent_Identity=29.7777777777778, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI87081733, Length=299, Percent_Identity=51.1705685618729, Blast_Score=288, Evalue=4e-79,
Organism=Escherichia coli, GI1787363, Length=303, Percent_Identity=39.2739273927393, Blast_Score=201, Evalue=6e-53,
Organism=Escherichia coli, GI1790522, Length=304, Percent_Identity=29.2763157894737, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1786891, Length=266, Percent_Identity=26.6917293233083, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1787878, Length=250, Percent_Identity=24.4, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000600 [H]

Pfam domain/function: PF00480 ROK [H]

EC number: =2.7.1.4 [H]

Molecular weight: Translated: 32111; Mature: 31980

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS01125 ROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDYRIGIDFGGTKIEIAALARKDGQELVRRRIVNPGYYDGAITAIRDLVAGVDTELGGQ
CCCEEEEEECCCCEEEEEEHHHCCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCC
GTVGIGIPGSISPDTGVIKNANATWLNNQPFGRDLTAAVGREVRVENDANCFALSEAVDG
CEEEECCCCCCCCCCCEEECCCCEEECCCCCCCHHHHHCCCEEEECCCCCEEEEHHHHCC
AGAGFGVVLGVIIGTGMGAGIIVNGKLLIGAHHIAGEWGHVPLPWPRIEEMPLPKCFCGN
CCCHHHHHHHHHHHCCCCCCEEEECEEEEEEHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
EGCLERYLSGSALAQDWKGPGNRSTAGIESAAEDGDQTAIGALDRYMDRMARACAMAINF
HHHHHHHHCCHHHHHHCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHC
LDPDVIVLGGGVSNLQSIYDRVPLLMPRYVITPRCNTPIVRNRHGDSSGVRGAAWLWSGD
CCCCEEEECCCHHHHHHHHHHHHHHCCCEEECCCCCCCCEECCCCCCCCCCEEEEEECCC
EGVGP
CCCCC
>Mature Secondary Structure 
TDYRIGIDFGGTKIEIAALARKDGQELVRRRIVNPGYYDGAITAIRDLVAGVDTELGGQ
CCEEEEEECCCCEEEEEEHHHCCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCC
GTVGIGIPGSISPDTGVIKNANATWLNNQPFGRDLTAAVGREVRVENDANCFALSEAVDG
CEEEECCCCCCCCCCCEEECCCCEEECCCCCCCHHHHHCCCEEEECCCCCEEEEHHHHCC
AGAGFGVVLGVIIGTGMGAGIIVNGKLLIGAHHIAGEWGHVPLPWPRIEEMPLPKCFCGN
CCCHHHHHHHHHHHCCCCCCEEEECEEEEEEHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
EGCLERYLSGSALAQDWKGPGNRSTAGIESAAEDGDQTAIGALDRYMDRMARACAMAINF
HHHHHHHHCCHHHHHHCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHC
LDPDVIVLGGGVSNLQSIYDRVPLLMPRYVITPRCNTPIVRNRHGDSSGVRGAAWLWSGD
CCCCEEEECCCHHHHHHHHHHHHHHCCCEEECCCCCCCCEECCCCCCCCCCEEEEEECCC
EGVGP
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8807285; 9278503; 1744033 [H]