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The map label for this gene is miaB

Identifier: 162147627

GI number: 162147627

Start: 1876756

End: 1878198

Strand: Reverse

Name: miaB

Synonym: GDI_1843

Alternate gene names: 162147627

Gene position: 1878198-1876756 (Counterclockwise)

Preceding gene: 162147630

Following gene: 162147626

Centisome position: 47.62

GC content: 68.61

Gene sequence:

>1443_bases
ATGGTCCGGCGGCGCGCCCAGGAAGTCTCAGCCGTGACCGATCAGCCTGCCTGCCCTCCCGCCATGGATCTGGCCCCCGC
GTCAGGATCGCGGGGCCTGCACGTCATCACGTGGGGCTGCCAGATGAATGTGTACGACAGCGCGCGGATGACCGACGTGC
TGCGCCCGCTGGGCTATCACCCGGTGGACACGCCCGATACCGCCGACATGGTCATCCTCAATACCTGCCATATCCGCGAC
CGCGCGGCGGAAAAGGTGTTTTCCGAACTGGGACGCCTGCGCCTGGTCAAGGAAGCCCGGGCGACGGAGGGACAGCAGAC
CGTGCTGGCCGTCGCCGGCTGCGTGGCGCAGGCCGAAGGCAAGGAGATCCTGGCCCGCGCGCCCTTCGTCGATATCGTTC
TGGGTCCGCAGACCTATCATCGCCTGCCGGAGATGGTGGCGCGCGCGGCGCGCGCCGCCGGCGCCGTGATCGACACCGAC
TTCCCGGCGGAACAGAAATTCGACTTCCTGCCGGATGCGCAGGCCCCGCAGAGCCCCGGCGGCATCACGTCGTTCCTGAC
CATCCAGGAAGGCTGCGACAAGTTCTGCTCGTTCTGCGTGGTGCCCTATACGCGCGGTGCCGAAGCCAGCCGCCCGGTGG
CGTCGGTGCTGCGCGAGGCCCGGCGCATGGTGGAATGCGGCGCGCGCGAGATCACGCTGCTGGGCCAGAACGTCAACGCC
TATCATGGCGAAGGGCCGGACGGCCGCGTGTGGGGCCTGGCGCGCCTGGCCGAGGCCCTGGCCGCCATCCCCGGCCTGGC
GCGCATCCGCTACACCACGTCGCACCCGCGCGACATGGATGCGGACCTGATCGCCGCCCATCGCGACCTGCCGGCGCTGA
TGCCATTCCTGCACCTGCCGGTGCAGTCGGGGTCCGACCGTATCCTGGATGCGATGAATCGCGGCCATACCGCCGCCGAT
TATCGCGACCTGGTGCTGCGCCTGCGCGACGCCCGGCCGGATATCGCGCTGTCGTCGGATTTCATCGTCGGCCATCCGGG
CGAAACCGACGCCGATTTCGAGGCGACCCTGCAACTGATCCGCGATGTCGGCTTCGCGCAGGCCTTCTCGTTCAAATATT
CCCCCCGCCCCGGCACGCCGGCCGCCGGCGCGCCGCTGCAAGTCGCGGAAGACGTCAAGGACGCCCGCCTGCAGGCGCTG
CAGGCCCTGCTGCGCACGCAGCAGGACGCGTTCAACGACGGGACGGTGGGACACGTGGTTCCGGTGCTGTTCACAGGGCA
CGGGCGCAAGGCGGGGCAGCTTTCGGGCCGTTCCCCCTACCTCCAGCCCGTGCATGTGGAAGGGCCGGACAGCCTGATCG
GCCAGATCGCAAATGTGGAGATCCGTGAGCGCTACACCAATTCGTTGAGTGGAACCCTCGTACAGGAGAGAGCCTTCGCT
TGA

Upstream 100 bases:

>100_bases
ACGATGATGCTGTCGTACCCCGGTTCGGCGGGCCATAGGCCCGGCAGAAGGCCTTGACCCGAAGCGCCACACATATGATG
TGTTCCCGCGACCGCGCGGG

Downstream 100 bases:

>100_bases
GAGAACAGACGGTATCAGCAATCCTGACCGGCCGGCGCCCCGCACGGGCGCAGGACCATCACGATCCGTCCCGGCATGGC
GGCATGGACGCCATGCGGAC

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MVRRRAQEVSAVTDQPACPPAMDLAPASGSRGLHVITWGCQMNVYDSARMTDVLRPLGYHPVDTPDTADMVILNTCHIRD
RAAEKVFSELGRLRLVKEARATEGQQTVLAVAGCVAQAEGKEILARAPFVDIVLGPQTYHRLPEMVARAARAAGAVIDTD
FPAEQKFDFLPDAQAPQSPGGITSFLTIQEGCDKFCSFCVVPYTRGAEASRPVASVLREARRMVECGAREITLLGQNVNA
YHGEGPDGRVWGLARLAEALAAIPGLARIRYTTSHPRDMDADLIAAHRDLPALMPFLHLPVQSGSDRILDAMNRGHTAAD
YRDLVLRLRDARPDIALSSDFIVGHPGETDADFEATLQLIRDVGFAQAFSFKYSPRPGTPAAGAPLQVAEDVKDARLQAL
QALLRTQQDAFNDGTVGHVVPVLFTGHGRKAGQLSGRSPYLQPVHVEGPDSLIGQIANVEIRERYTNSLSGTLVQERAFA

Sequences:

>Translated_480_residues
MVRRRAQEVSAVTDQPACPPAMDLAPASGSRGLHVITWGCQMNVYDSARMTDVLRPLGYHPVDTPDTADMVILNTCHIRD
RAAEKVFSELGRLRLVKEARATEGQQTVLAVAGCVAQAEGKEILARAPFVDIVLGPQTYHRLPEMVARAARAAGAVIDTD
FPAEQKFDFLPDAQAPQSPGGITSFLTIQEGCDKFCSFCVVPYTRGAEASRPVASVLREARRMVECGAREITLLGQNVNA
YHGEGPDGRVWGLARLAEALAAIPGLARIRYTTSHPRDMDADLIAAHRDLPALMPFLHLPVQSGSDRILDAMNRGHTAAD
YRDLVLRLRDARPDIALSSDFIVGHPGETDADFEATLQLIRDVGFAQAFSFKYSPRPGTPAAGAPLQVAEDVKDARLQAL
QALLRTQQDAFNDGTVGHVVPVLFTGHGRKAGQLSGRSPYLQPVHVEGPDSLIGQIANVEIRERYTNSLSGTLVQERAFA
>Mature_480_residues
MVRRRAQEVSAVTDQPACPPAMDLAPASGSRGLHVITWGCQMNVYDSARMTDVLRPLGYHPVDTPDTADMVILNTCHIRD
RAAEKVFSELGRLRLVKEARATEGQQTVLAVAGCVAQAEGKEILARAPFVDIVLGPQTYHRLPEMVARAARAAGAVIDTD
FPAEQKFDFLPDAQAPQSPGGITSFLTIQEGCDKFCSFCVVPYTRGAEASRPVASVLREARRMVECGAREITLLGQNVNA
YHGEGPDGRVWGLARLAEALAAIPGLARIRYTTSHPRDMDADLIAAHRDLPALMPFLHLPVQSGSDRILDAMNRGHTAAD
YRDLVLRLRDARPDIALSSDFIVGHPGETDADFEATLQLIRDVGFAQAFSFKYSPRPGTPAAGAPLQVAEDVKDARLQAL
QALLRTQQDAFNDGTVGHVVPVLFTGHGRKAGQLSGRSPYLQPVHVEGPDSLIGQIANVEIRERYTNSLSGTLVQERAFA

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872782, Length=485, Percent_Identity=33.1958762886598, Blast_Score=253, Evalue=3e-67,
Organism=Homo sapiens, GI28872784, Length=460, Percent_Identity=33.9130434782609, Blast_Score=252, Evalue=6e-67,
Organism=Homo sapiens, GI93277076, Length=494, Percent_Identity=26.7206477732794, Blast_Score=163, Evalue=3e-40,
Organism=Escherichia coli, GI1786882, Length=448, Percent_Identity=48.4375, Blast_Score=426, Evalue=1e-120,
Organism=Escherichia coli, GI1787057, Length=378, Percent_Identity=31.2169312169312, Blast_Score=174, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI17553146, Length=431, Percent_Identity=34.338747099768, Blast_Score=235, Evalue=3e-62,
Organism=Caenorhabditis elegans, GI71996771, Length=342, Percent_Identity=27.1929824561404, Blast_Score=108, Evalue=9e-24,
Organism=Drosophila melanogaster, GI21356207, Length=425, Percent_Identity=32.7058823529412, Blast_Score=249, Evalue=2e-66,
Organism=Drosophila melanogaster, GI19922432, Length=490, Percent_Identity=27.5510204081633, Blast_Score=146, Evalue=3e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_GLUDA (A9HIM2)

Other databases:

- EMBL:   AM889285
- EMBL:   CP001189
- RefSeq:   YP_001602088.1
- RefSeq:   YP_002274487.1
- ProteinModelPortal:   A9HIM2
- SMR:   A9HIM2
- GeneID:   5789437
- GeneID:   6973459
- GenomeReviews:   AM889285_GR
- GenomeReviews:   CP001189_GR
- KEGG:   gdj:Gdia_0070
- HOGENOM:   HBG457663
- OMA:   ANLHDDT
- ProtClustDB:   PRK14325
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 52008; Mature: 52008

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRRRAQEVSAVTDQPACPPAMDLAPASGSRGLHVITWGCQMNVYDSARMTDVLRPLGYH
CCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHCCCC
PVDTPDTADMVILNTCHIRDRAAEKVFSELGRLRLVKEARATEGQQTVLAVAGCVAQAEG
CCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
KEILARAPFVDIVLGPQTYHRLPEMVARAARAAGAVIDTDFPAEQKFDFLPDAQAPQSPG
HHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCCC
GITSFLTIQEGCDKFCSFCVVPYTRGAEASRPVASVLREARRMVECGAREITLLGQNVNA
CCCHHHHHHHHHHHHHHEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCC
YHGEGPDGRVWGLARLAEALAAIPGLARIRYTTSHPRDMDADLIAAHRDLPALMPFLHLP
CCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHCCCHHHHHHHHCC
VQSGSDRILDAMNRGHTAADYRDLVLRLRDARPDIALSSDFIVGHPGETDADFEATLQLI
CCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEECCCEEEECCCCCCCCHHHHHHHH
RDVGFAQAFSFKYSPRPGTPAAGAPLQVAEDVKDARLQALQALLRTQQDAFNDGTVGHVV
HHCCHHHHHEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHEE
PVLFTGHGRKAGQLSGRSPYLQPVHVEGPDSLIGQIANVEIRERYTNSLSGTLVQERAFA
EEEEECCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVRRRAQEVSAVTDQPACPPAMDLAPASGSRGLHVITWGCQMNVYDSARMTDVLRPLGYH
CCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHCCCC
PVDTPDTADMVILNTCHIRDRAAEKVFSELGRLRLVKEARATEGQQTVLAVAGCVAQAEG
CCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
KEILARAPFVDIVLGPQTYHRLPEMVARAARAAGAVIDTDFPAEQKFDFLPDAQAPQSPG
HHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCCC
GITSFLTIQEGCDKFCSFCVVPYTRGAEASRPVASVLREARRMVECGAREITLLGQNVNA
CCCHHHHHHHHHHHHHHEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCC
YHGEGPDGRVWGLARLAEALAAIPGLARIRYTTSHPRDMDADLIAAHRDLPALMPFLHLP
CCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHCCCHHHHHHHHCC
VQSGSDRILDAMNRGHTAADYRDLVLRLRDARPDIALSSDFIVGHPGETDADFEATLQLI
CCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEECCCEEEECCCCCCCCHHHHHHHH
RDVGFAQAFSFKYSPRPGTPAAGAPLQVAEDVKDARLQALQALLRTQQDAFNDGTVGHVV
HHCCHHHHHEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHEE
PVLFTGHGRKAGQLSGRSPYLQPVHVEGPDSLIGQIANVEIRERYTNSLSGTLVQERAFA
EEEEECCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA