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The map label for this gene is mltB [H]

Identifier: 162147579

GI number: 162147579

Start: 1826557

End: 1827549

Strand: Direct

Name: mltB [H]

Synonym: GDI_1795

Alternate gene names: 162147579

Gene position: 1826557-1827549 (Clockwise)

Preceding gene: 162147576

Following gene: 162147580

Centisome position: 46.31

GC content: 70.09

Gene sequence:

>993_bases
ATGATCCGACGACGTGACTTCCTGATGTCAGCCTCGGCCACCTGCGCGGCCGGTGGCATCGGCATGGCCGCGTCGGCGCG
GGCCGACAGCGCCCCTGCTGCATCCTATGCGGCCTTCCTGGCCGGGGTGCGGGCCGAGGCCGTTTCCCATGGCCTGTCGG
CCGCGATCGTCGATCGCGCCCTGGCGCTGGAGGCGCCGAACGCGCGCGTCCTGCAACTGGACCAGCACCAGCCCGAATTC
ACCCTGACCTGGGCGCAGTACCGACAGCGCGTGCTGACGCCCAAGAAGGTCGCCGACGGCCGCGTGGCGGCGGGGGCACG
GCGCGCGCTGCTGGCGGAGGTCGGCCGGCATTTCGGCGTGGACCCGCAGCCGCTGATGGGAATCTGGGGGTTGGAATCCG
CCTACGGCACGCGTACGGGCACCTTCCAGATCCCCGACGCCCTGGCGACCCTGGCCTATGACGGGCGGCGCGCGTCGTTC
TTTCGCGGCGAACTGATGAATGCGCTGCGCATCCTGGACCATGGCGACGTCACGCCCGATTCCATGACCGGCAGCTATGC
CGGTGCGATGGGACAGCCGCAGTTCATGCCCAGCGCCTATCTGCGCTACGCCGTCGACTATACCGGCAGCGGCCGGCGCG
ACATCTGGACCAGCGAGCCCGACGTCTTCGCCTCGATCGCCAATTACCTGGCCCGTTGCGGCTGGTCGGCCGGCGAGCCC
TGGGGCCAGGAAATCTCGGTTCCCACGCGGCTGGCGCAGGCCGATCTGGGGCGGGGCAGGGTGCGTACCCTGGCCGACTG
GATGGCGCAGGGCGTGCGGCGGCTGGACGGGCGGCGTTTCTCGCGCCCCGATATCAGCGGCGCGGTGATCCGGCCGGACG
GACCGGGCGGCGAGGCCTTCATGGTCTATCGCAATTTTTCGGTCATCCGGCGCTACAATCCATCCGATTTCTACGCGCTG
GCGGTCGGGCTGCTGGGCCACGACGTCACGTGA

Upstream 100 bases:

>100_bases
AAATCGCCTGCGGGCGACGGCAAATTCCGCCGCCGGACAGCGCGTGGTTGCCCCAGGCAAGGATTGAAAGGCCGGCGGGG
AATGTGGCACAGCTTGCATC

Downstream 100 bases:

>100_bases
CGCGCCGGGCCGTTCCCACGGTAATGGCCGCGCTGGGACTGCTGGGCTGCCATCGGGCGGAGCCCGGGCTGCCCCCTGCG
GCGCCGCATTACGTCGTCGG

Product: membrane-bound lytic murein transglycosylase B

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MIRRRDFLMSASATCAAGGIGMAASARADSAPAASYAAFLAGVRAEAVSHGLSAAIVDRALALEAPNARVLQLDQHQPEF
TLTWAQYRQRVLTPKKVADGRVAAGARRALLAEVGRHFGVDPQPLMGIWGLESAYGTRTGTFQIPDALATLAYDGRRASF
FRGELMNALRILDHGDVTPDSMTGSYAGAMGQPQFMPSAYLRYAVDYTGSGRRDIWTSEPDVFASIANYLARCGWSAGEP
WGQEISVPTRLAQADLGRGRVRTLADWMAQGVRRLDGRRFSRPDISGAVIRPDGPGGEAFMVYRNFSVIRRYNPSDFYAL
AVGLLGHDVT

Sequences:

>Translated_330_residues
MIRRRDFLMSASATCAAGGIGMAASARADSAPAASYAAFLAGVRAEAVSHGLSAAIVDRALALEAPNARVLQLDQHQPEF
TLTWAQYRQRVLTPKKVADGRVAAGARRALLAEVGRHFGVDPQPLMGIWGLESAYGTRTGTFQIPDALATLAYDGRRASF
FRGELMNALRILDHGDVTPDSMTGSYAGAMGQPQFMPSAYLRYAVDYTGSGRRDIWTSEPDVFASIANYLARCGWSAGEP
WGQEISVPTRLAQADLGRGRVRTLADWMAQGVRRLDGRRFSRPDISGAVIRPDGPGGEAFMVYRNFSVIRRYNPSDFYAL
AVGLLGHDVT
>Mature_330_residues
MIRRRDFLMSASATCAAGGIGMAASARADSAPAASYAAFLAGVRAEAVSHGLSAAIVDRALALEAPNARVLQLDQHQPEF
TLTWAQYRQRVLTPKKVADGRVAAGARRALLAEVGRHFGVDPQPLMGIWGLESAYGTRTGTFQIPDALATLAYDGRRASF
FRGELMNALRILDHGDVTPDSMTGSYAGAMGQPQFMPSAYLRYAVDYTGSGRRDIWTSEPDVFASIANYLARCGWSAGEP
WGQEISVPTRLAQADLGRGRVRTLADWMAQGVRRLDGRRFSRPDISGAVIRPDGPGGEAFMVYRNFSVIRRYNPSDFYAL
AVGLLGHDVT

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=263, Percent_Identity=32.319391634981, Blast_Score=123, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 35707; Mature: 35707

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRRRDFLMSASATCAAGGIGMAASARADSAPAASYAAFLAGVRAEAVSHGLSAAIVDRA
CCCCHHHHHCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHH
LALEAPNARVLQLDQHQPEFTLTWAQYRQRVLTPKKVADGRVAAGARRALLAEVGRHFGV
HHCCCCCCEEEEECCCCCCEEEEHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHCCC
DPQPLMGIWGLESAYGTRTGTFQIPDALATLAYDGRRASFFRGELMNALRILDHGDVTPD
CCCHHHHHCCCCHHCCCCCCCEECCHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCC
SMTGSYAGAMGQPQFMPSAYLRYAVDYTGSGRRDIWTSEPDVFASIANYLARCGWSAGEP
CCCCCCCCCCCCCCCCHHHHHHEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCC
WGQEISVPTRLAQADLGRGRVRTLADWMAQGVRRLDGRRFSRPDISGAVIRPDGPGGEAF
CCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCEE
MVYRNFSVIRRYNPSDFYALAVGLLGHDVT
EEEECEEEEEECCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIRRRDFLMSASATCAAGGIGMAASARADSAPAASYAAFLAGVRAEAVSHGLSAAIVDRA
CCCCHHHHHCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHH
LALEAPNARVLQLDQHQPEFTLTWAQYRQRVLTPKKVADGRVAAGARRALLAEVGRHFGV
HHCCCCCCEEEEECCCCCCEEEEHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHCCC
DPQPLMGIWGLESAYGTRTGTFQIPDALATLAYDGRRASFFRGELMNALRILDHGDVTPD
CCCHHHHHCCCCHHCCCCCCCEECCHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCC
SMTGSYAGAMGQPQFMPSAYLRYAVDYTGSGRRDIWTSEPDVFASIANYLARCGWSAGEP
CCCCCCCCCCCCCCCCHHHHHHEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCC
WGQEISVPTRLAQADLGRGRVRTLADWMAQGVRRLDGRRFSRPDISGAVIRPDGPGGEAF
CCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCEE
MVYRNFSVIRRYNPSDFYALAVGLLGHDVT
EEEECEEEEEECCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]