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The map label for this gene is thiG

Identifier: 162147565

GI number: 162147565

Start: 1810882

End: 1811652

Strand: Direct

Name: thiG

Synonym: GDI_1781

Alternate gene names: 162147565

Gene position: 1810882-1811652 (Clockwise)

Preceding gene: 162147564

Following gene: 162147566

Centisome position: 45.91

GC content: 69.13

Gene sequence:

>771_bases
ATGCTGTTTTATGGAACCGAACTGAAATCGCGCCTGATGCTGGGCACCGCGCAATATCCGTCGCCCGAGATCCTGTCCGA
CGCGGTGCGCGCCGCCGAGGCCGGTGTGGTGACGGTGTCGTTGCGCCGCGAGTCCGCGGGCCAGAGGGCCGGTCAGGCCT
TCTGGTCGATGATCCGGGACCTGGGCGTGCCGGTGCTGCCCAATACGGCGGGCTGCCATACGGTCAAGGAAGCGGTGACC
ACCGCCCACATGGCCCGCGAGGTATTCGACACCGACTGGATCAAGCTGGAGGTCATCGGCGAGTCAGACACGCTGCAGCC
CGACATGTTCGGGCTGGTCGAGGCCGCGCGCATCCTGTCCCGGGACGGGTTCAAGGTGTTTCCCTACATGACCGAGGACC
TGGTGGGCGCCGAACGCCTGCTGCAGGCGGGATGCGAGGTCCTGATGCCCTGGGGCGCGCCCATCGGGTCGGGCAAGGGG
CTGAACAACGTGTTCGGCCTGCGCGCGCTGCGGGCGCATTTCCCCGACGTGCCGCTGGTCGTCGATGCCGGGATCGGCCT
GCCGTCGCACGCGGCGCAGGCGATGGAACTGGGCTATGACGCCGTGCTGATCAACACCGCCGTGGCCAAGGCCGGCGACC
CGGTGCGCATGGCCCGCGCATTCCGCCTGGCGGTCGAGGCCGGGCTGATCGCGCGCGAGGCCGACCCGATTGAGGAACGT
GACATGGCCGCACCCTCGACCCCGGTGCTGGGCCGGGCGATGCTGGCATGA

Upstream 100 bases:

>100_bases
GTCAACGGTGATTTCGTGCCCGTTTCGCGCCGTGCCGCGCTGACGCTGGACGAGGGGGCGCGGGTCGAAATCCTGGCCCC
CATGCAGGGGGGCTGAGCCC

Downstream 100 bases:

>100_bases
GGGCTCTGGCATGAGGAAGCTGGTATGAGGAAGCTGGTATGAGCCTGGCGTCGCGTATCTATCCCGTTGTCGACCGGGCG
GACTGGATCGACCGGCTGGG

Product: thiazole synthase

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MLFYGTELKSRLMLGTAQYPSPEILSDAVRAAEAGVVTVSLRRESAGQRAGQAFWSMIRDLGVPVLPNTAGCHTVKEAVT
TAHMAREVFDTDWIKLEVIGESDTLQPDMFGLVEAARILSRDGFKVFPYMTEDLVGAERLLQAGCEVLMPWGAPIGSGKG
LNNVFGLRALRAHFPDVPLVVDAGIGLPSHAAQAMELGYDAVLINTAVAKAGDPVRMARAFRLAVEAGLIAREADPIEER
DMAAPSTPVLGRAMLA

Sequences:

>Translated_256_residues
MLFYGTELKSRLMLGTAQYPSPEILSDAVRAAEAGVVTVSLRRESAGQRAGQAFWSMIRDLGVPVLPNTAGCHTVKEAVT
TAHMAREVFDTDWIKLEVIGESDTLQPDMFGLVEAARILSRDGFKVFPYMTEDLVGAERLLQAGCEVLMPWGAPIGSGKG
LNNVFGLRALRAHFPDVPLVVDAGIGLPSHAAQAMELGYDAVLINTAVAKAGDPVRMARAFRLAVEAGLIAREADPIEER
DMAAPSTPVLGRAMLA
>Mature_256_residues
MLFYGTELKSRLMLGTAQYPSPEILSDAVRAAEAGVVTVSLRRESAGQRAGQAFWSMIRDLGVPVLPNTAGCHTVKEAVT
TAHMAREVFDTDWIKLEVIGESDTLQPDMFGLVEAARILSRDGFKVFPYMTEDLVGAERLLQAGCEVLMPWGAPIGSGKG
LNNVFGLRALRAHFPDVPLVVDAGIGLPSHAAQAMELGYDAVLINTAVAKAGDPVRMARAFRLAVEAGLIAREADPIEER
DMAAPSTPVLGRAMLA

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family

Homologues:

Organism=Escherichia coli, GI48994993, Length=244, Percent_Identity=44.672131147541, Blast_Score=199, Evalue=2e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIG_GLUDA (A9HI56)

Other databases:

- EMBL:   AM889285
- EMBL:   CP001189
- RefSeq:   YP_001602026.1
- RefSeq:   YP_002274428.1
- ProteinModelPortal:   A9HI56
- SMR:   A9HI56
- GeneID:   5790034
- GeneID:   6973399
- GenomeReviews:   AM889285_GR
- GenomeReviews:   CP001189_GR
- KEGG:   gdj:Gdia_0011
- HOGENOM:   HBG296821
- OMA:   VAIRRTN
- ProtClustDB:   PRK00208
- GO:   GO:0005737
- HAMAP:   MF_00443
- InterPro:   IPR013785
- InterPro:   IPR008867
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF05690 ThiG; SSF110399 ThiG

EC number: NA

Molecular weight: Translated: 27401; Mature: 27401

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: NA

Important sites: ACT_SITE 95-95 BINDING 156-156

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFYGTELKSRLMLGTAQYPSPEILSDAVRAAEAGVVTVSLRRESAGQRAGQAFWSMIRD
CEEECCCHHHHHHCCCCCCCCHHHHHHHHHHHHCCEEEEEECHHHHHHHHHHHHHHHHHH
LGVPVLPNTAGCHTVKEAVTTAHMAREVFDTDWIKLEVIGESDTLQPDMFGLVEAARILS
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHH
RDGFKVFPYMTEDLVGAERLLQAGCEVLMPWGAPIGSGKGLNNVFGLRALRAHFPDVPLV
CCCCEECCCHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCEE
VDAGIGLPSHAAQAMELGYDAVLINTAVAKAGDPVRMARAFRLAVEAGLIAREADPIEER
EECCCCCCHHHHHHHHHCCHHEEEHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHH
DMAAPSTPVLGRAMLA
HCCCCCCCCHHHHHCC
>Mature Secondary Structure
MLFYGTELKSRLMLGTAQYPSPEILSDAVRAAEAGVVTVSLRRESAGQRAGQAFWSMIRD
CEEECCCHHHHHHCCCCCCCCHHHHHHHHHHHHCCEEEEEECHHHHHHHHHHHHHHHHHH
LGVPVLPNTAGCHTVKEAVTTAHMAREVFDTDWIKLEVIGESDTLQPDMFGLVEAARILS
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHH
RDGFKVFPYMTEDLVGAERLLQAGCEVLMPWGAPIGSGKGLNNVFGLRALRAHFPDVPLV
CCCCEECCCHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCEE
VDAGIGLPSHAAQAMELGYDAVLINTAVAKAGDPVRMARAFRLAVEAGLIAREADPIEER
EECCCCCCHHHHHHHHHCCHHEEEHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHH
DMAAPSTPVLGRAMLA
HCCCCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA