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The map label for this gene is rpoE [H]

Identifier: 162147527

GI number: 162147527

Start: 1765051

End: 1765623

Strand: Direct

Name: rpoE [H]

Synonym: GDI_1743

Alternate gene names: 162147527

Gene position: 1765051-1765623 (Clockwise)

Preceding gene: 162147526

Following gene: 162147528

Centisome position: 44.75

GC content: 65.97

Gene sequence:

>573_bases
ATGACGAACAATTTTCATGACCAGGTCATCTCGATCCTTCCCCGCCTGCGGGTCCAGGCCCTCGCCCTGACGCGCAACAG
GACGATGGCGGAGGATCTGGTGCAGGATGCCGTATGCAACGCGTTGGCGGCCAAGGAGAGCTTCATCCCCGGCACAAATT
TTTCGGCATGGATGCACCGCATCCTGCGCAACCGTTTCATCTCCGACCTGCGCAAGCGGCGGGAGACGACGGATATCGAG
GACGTGCCTGCGGCGGCGCTGGCGACTTCGGCCTCGCACGAGGACAGGCTGGCGCTGAAGGACCTGGCGCTGGCGATGGA
TCGCCTTCCCGACGATCAGCGCGAAGCGCTGATCATGGTGGTCCTGCACGGCATGAGCTACGAATCCCTTGCCGAGGCGA
CGGGGTGCGCGGTCGGCACGGCCAAAAGCCGCGTGTTCCGGGCCCGCCGGCAACTGGAGAGCTGGATGATGGGCGAATAT
GACAGCACCCAGGTCAAGATCCGTGCGGCCCGGCTGAGGGGGCTTCTGGCCGAACGGACCGCCGCGCAGGGGGCAGCCGC
ACCCCGCCCCTGA

Upstream 100 bases:

>100_bases
GACGGGGCGAAAAGCGGACCTCACGTCTTCGTTATGACAAAAACGCAACCCTGTCGCGCCGCCGTTCATTGTTTCGGATG
TGTGAAGGGAACCGAAACCA

Downstream 100 bases:

>100_bases
AAAGAGGCAGGGGGGCTGACGGCGCCTTGCCATAATTTGGTCGATATTGATCGGGCAGTGGCTGGACTGTCCGCGGGGGG
TGATTTACGGTTTGTAAACA

Product: RNA polymerase sigma-E factor (sigma-24)

Products: NA

Alternate protein names: Sigma-24 [H]

Number of amino acids: Translated: 190; Mature: 189

Protein sequence:

>190_residues
MTNNFHDQVISILPRLRVQALALTRNRTMAEDLVQDAVCNALAAKESFIPGTNFSAWMHRILRNRFISDLRKRRETTDIE
DVPAAALATSASHEDRLALKDLALAMDRLPDDQREALIMVVLHGMSYESLAEATGCAVGTAKSRVFRARRQLESWMMGEY
DSTQVKIRAARLRGLLAERTAAQGAAAPRP

Sequences:

>Translated_190_residues
MTNNFHDQVISILPRLRVQALALTRNRTMAEDLVQDAVCNALAAKESFIPGTNFSAWMHRILRNRFISDLRKRRETTDIE
DVPAAALATSASHEDRLALKDLALAMDRLPDDQREALIMVVLHGMSYESLAEATGCAVGTAKSRVFRARRQLESWMMGEY
DSTQVKIRAARLRGLLAERTAAQGAAAPRP
>Mature_189_residues
TNNFHDQVISILPRLRVQALALTRNRTMAEDLVQDAVCNALAAKESFIPGTNFSAWMHRILRNRFISDLRKRRETTDIED
VPAAALATSASHEDRLALKDLALAMDRLPDDQREALIMVVLHGMSYESLAEATGCAVGTAKSRVFRARRQLESWMMGEYD
STQVKIRAARLRGLLAERTAAQGAAAPRP

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in heat shock and oxidative stress response; it is believed to control protein processing

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=136, Percent_Identity=28.6764705882353, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014293
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR013325
- InterPro:   IPR013324 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 21175; Mature: 21043

Theoretical pI: Translated: 9.80; Mature: 9.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNNFHDQVISILPRLRVQALALTRNRTMAEDLVQDAVCNALAAKESFIPGTNFSAWMHR
CCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
ILRNRFISDLRKRRETTDIEDVPAAALATSASHEDRLALKDLALAMDRLPDDQREALIMV
HHHHHHHHHHHHHHCCCCHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHH
VLHGMSYESLAEATGCAVGTAKSRVFRARRQLESWMMGEYDSTQVKIRAARLRGLLAERT
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
AAQGAAAPRP
HHCCCCCCCC
>Mature Secondary Structure 
TNNFHDQVISILPRLRVQALALTRNRTMAEDLVQDAVCNALAAKESFIPGTNFSAWMHR
CCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
ILRNRFISDLRKRRETTDIEDVPAAALATSASHEDRLALKDLALAMDRLPDDQREALIMV
HHHHHHHHHHHHHHCCCCHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHH
VLHGMSYESLAEATGCAVGTAKSRVFRARRQLESWMMGEYDSTQVKIRAARLRGLLAERT
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
AAQGAAAPRP
HHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]