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The map label for this gene is mscL [H]

Identifier: 162147515

GI number: 162147515

Start: 1751777

End: 1752241

Strand: Direct

Name: mscL [H]

Synonym: GDI_1731

Alternate gene names: 162147515

Gene position: 1751777-1752241 (Clockwise)

Preceding gene: 162147511

Following gene: 162147518

Centisome position: 44.41

GC content: 63.66

Gene sequence:

>465_bases
ATGGCCACGAAGATCCCGTCCATCCACGCCCCGGGATGGATTTCCGAATTCCGGACGTTCCTCATGCGGGGGAACGTCGT
CGACCTGGCGGTCGGTGTCATCATCGGCGCCGCTTTCACCGGGATCGTCAACAGCCTGGTCAAGGACATCTTTACGCCGT
TCATCGGACTGCTGATCGGCGGCATCGACTTCACCAACGTCTTCTTCACCCTGCGCGGGCCGCATCTGCCCACCCTGGCC
GACGCACAGAAGGCCGGGGCGGTCACGATCAATCTGGGCCTGTTCCTGAACGCCGTCATCCAGTTCGTCATCATCGGCTT
CGTGATCTTCTGGGTGGTCAAGGGCGTGAACAAGCTGACTGTGAAGCACGAAGCCCCGGCCGCGCCGCCCGCGCCGCCGC
GCAGCGAAGTGCTGCTGCAGGAAATCCGCGACCTGCTGGCCACCAGGGAAAAGACGGCGCCCTGA

Upstream 100 bases:

>100_bases
GGGCGCCACGCCGGCGGCGGGAATGCGGTAACGTGCTGTTTTCCTGTTCCTGCGACGGTCGGCGGATTGCTATCAGGGTA
AGCCAAAAGAAGGAGGCTCC

Downstream 100 bases:

>100_bases
CGTCCGGCCCGTCCGGTGGGCGCGGGGCATGACCTCGCGCCCACCGGGCGTTACAGGCGCTGGATGCGACGGGCCTCGCG
CGCATGGTCGCGCAGGATTT

Product: large-conductance mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 154; Mature: 153

Protein sequence:

>154_residues
MATKIPSIHAPGWISEFRTFLMRGNVVDLAVGVIIGAAFTGIVNSLVKDIFTPFIGLLIGGIDFTNVFFTLRGPHLPTLA
DAQKAGAVTINLGLFLNAVIQFVIIGFVIFWVVKGVNKLTVKHEAPAAPPAPPRSEVLLQEIRDLLATREKTAP

Sequences:

>Translated_154_residues
MATKIPSIHAPGWISEFRTFLMRGNVVDLAVGVIIGAAFTGIVNSLVKDIFTPFIGLLIGGIDFTNVFFTLRGPHLPTLA
DAQKAGAVTINLGLFLNAVIQFVIIGFVIFWVVKGVNKLTVKHEAPAAPPAPPRSEVLLQEIRDLLATREKTAP
>Mature_153_residues
ATKIPSIHAPGWISEFRTFLMRGNVVDLAVGVIIGAAFTGIVNSLVKDIFTPFIGLLIGGIDFTNVFFTLRGPHLPTLAD
AQKAGAVTINLGLFLNAVIQFVIIGFVIFWVVKGVNKLTVKHEAPAAPPAPPRSEVLLQEIRDLLATREKTAP

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell [H]

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family [H]

Homologues:

Organism=Escherichia coli, GI1789686, Length=141, Percent_Identity=55.3191489361702, Blast_Score=136, Evalue=8e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019823
- InterPro:   IPR001185
- InterPro:   IPR015089 [H]

Pfam domain/function: PF01741 MscL [H]

EC number: NA

Molecular weight: Translated: 16570; Mature: 16439

Theoretical pI: Translated: 10.39; Mature: 10.39

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATKIPSIHAPGWISEFRTFLMRGNVVDLAVGVIIGAAFTGIVNSLVKDIFTPFIGLLIG
CCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GIDFTNVFFTLRGPHLPTLADAQKAGAVTINLGLFLNAVIQFVIIGFVIFWVVKGVNKLT
CCCHHHHEEEECCCCCCCHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCHHEE
VKHEAPAAPPAPPRSEVLLQEIRDLLATREKTAP
ECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ATKIPSIHAPGWISEFRTFLMRGNVVDLAVGVIIGAAFTGIVNSLVKDIFTPFIGLLIG
CCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GIDFTNVFFTLRGPHLPTLADAQKAGAVTINLGLFLNAVIQFVIIGFVIFWVVKGVNKLT
CCCHHHHEEEECCCCCCCHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCHHEE
VKHEAPAAPPAPPRSEVLLQEIRDLLATREKTAP
ECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA