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The map label for this gene is insK [C]

Identifier: 162147342

GI number: 162147342

Start: 1556027

End: 1556854

Strand: Reverse

Name: insK [C]

Synonym: GDI_1552

Alternate gene names: 162147342

Gene position: 1556854-1556027 (Counterclockwise)

Preceding gene: 162147343

Following gene: 162147334

Centisome position: 39.47

GC content: 63.41

Gene sequence:

>828_bases
GTGACCTGGGCGATCCGGGAGAAAGAGTATTCGCAGCGACGGGCCTGCCGGCTGATCGGCATGGACCCGAAGACCTGGCG
CTATGCGTCACGCCGCCCGGATGATGCCGCAGCGCGCGGGCGGCTGCGCGAACTGGCTGGGGAGCGACGGCGATTTGGCT
ACCGGCGACTGCATATCCTGCTCGGCCGGGAAGGAATGACGATGAACCACAAGAAGCTGTTCCGGCTGTATCGCGAAGAG
GGGCTGTCGGTCCGCAAGCGTGGCGGCCGGAAACGGGCGCTGGGCACGCGCTCGCCGATGATGCTGCCCGACGGGCCGAA
CCAGCGCTGGAGCCTGGATTTCGTCTCGGATGCATTGAACAACGGACGGCGCTTCCGGGTGCTGACGGTGGTCGACGACT
ACACGCGCGAATGTCTGGCGCTGGTGGCGGACACCTCGTTATCAGGCGAACGCCTCGGTCGTGAACTCGACCGGATCGGC
GAGCATCGCGGCTGGCCGCTGATGATCGTTAGCGACAATGGCACCGAGATGACATCGAACGCGATCCTGGCCTGGCAGCA
GAAGCGATCGGTGCTGTGGCACTATATCGCACCGGGCAAGCCGCAGCAGAACGGGTTCGTCGAGAGCTTCAACGGCCGGT
TCCGCGACGAATGCCTCAATGAGCATCTGTTCCGTAACATCGCCCACGCTCGGACGGTCATCGAGGACTGGCGGGCCGAC
TACAACGCCGTCAGGCCTCACACCAGCCTCAATGGCATGACGCCAGAGGCTTTCGCTCAACACGCCACCAAGGCATACAA
CAATACACAGACCCTAACTCAAAACTGA

Upstream 100 bases:

>100_bases
CGAAGCTGAAGCGGCTTCTGGCGGAGAGCGTGATGGACGTCTCGACGCTGAAGGAACTACTGGCAAAAAACTCGTGACGC
CCGGTTTGCGGCGGGAAGCC

Downstream 100 bases:

>100_bases
GGGCACGTTCGGGGCAGGGTCAGACCATCTGTGCGGCATGTTTGAGCATGTATCCGACGAATGCGATGACGTGGAAGCCT
GCGAGCGTGGTCGCGTATCT

Product: integrase

Products: NA

Alternate protein names: ORF1 [H]

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MTWAIREKEYSQRRACRLIGMDPKTWRYASRRPDDAAARGRLRELAGERRRFGYRRLHILLGREGMTMNHKKLFRLYREE
GLSVRKRGGRKRALGTRSPMMLPDGPNQRWSLDFVSDALNNGRRFRVLTVVDDYTRECLALVADTSLSGERLGRELDRIG
EHRGWPLMIVSDNGTEMTSNAILAWQQKRSVLWHYIAPGKPQQNGFVESFNGRFRDECLNEHLFRNIAHARTVIEDWRAD
YNAVRPHTSLNGMTPEAFAQHATKAYNNTQTLTQN

Sequences:

>Translated_275_residues
MTWAIREKEYSQRRACRLIGMDPKTWRYASRRPDDAAARGRLRELAGERRRFGYRRLHILLGREGMTMNHKKLFRLYREE
GLSVRKRGGRKRALGTRSPMMLPDGPNQRWSLDFVSDALNNGRRFRVLTVVDDYTRECLALVADTSLSGERLGRELDRIG
EHRGWPLMIVSDNGTEMTSNAILAWQQKRSVLWHYIAPGKPQQNGFVESFNGRFRDECLNEHLFRNIAHARTVIEDWRAD
YNAVRPHTSLNGMTPEAFAQHATKAYNNTQTLTQN
>Mature_274_residues
TWAIREKEYSQRRACRLIGMDPKTWRYASRRPDDAAARGRLRELAGERRRFGYRRLHILLGREGMTMNHKKLFRLYREEG
LSVRKRGGRKRALGTRSPMMLPDGPNQRWSLDFVSDALNNGRRFRVLTVVDDYTRECLALVADTSLSGERLGRELDRIGE
HRGWPLMIVSDNGTEMTSNAILAWQQKRSVLWHYIAPGKPQQNGFVESFNGRFRDECLNEHLFRNIAHARTVIEDWRADY
NAVRPHTSLNGMTPEAFAQHATKAYNNTQTLTQN

Specific function: Involved in the transposition of the insertion sequence is150 [C]

COG id: COG2801

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1786462, Length=173, Percent_Identity=28.3236994219653, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1789981, Length=242, Percent_Identity=26.0330578512397, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1790724, Length=256, Percent_Identity=24.609375, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1789424, Length=256, Percent_Identity=24.609375, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1789224, Length=256, Percent_Identity=24.609375, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1788305, Length=256, Percent_Identity=24.609375, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1787669, Length=256, Percent_Identity=24.609375, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1786558, Length=256, Percent_Identity=24.609375, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 31989; Mature: 31858

Theoretical pI: Translated: 10.91; Mature: 10.91

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTWAIREKEYSQRRACRLIGMDPKTWRYASRRPDDAAARGRLRELAGERRRFGYRRLHIL
CCCCCCCHHHHHHHHHEEECCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEE
LGREGMTMNHKKLFRLYREEGLSVRKRGGRKRALGTRSPMMLPDGPNQRWSLDFVSDALN
ECCCCCCCCHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHC
NGRRFRVLTVVDDYTRECLALVADTSLSGERLGRELDRIGEHRGWPLMIVSDNGTEMTSN
CCCEEEEEEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCEECCC
AILAWQQKRSVLWHYIAPGKPQQNGFVESFNGRFRDECLNEHLFRNIAHARTVIEDWRAD
HHEEEHHHCCEEEEEECCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
YNAVRPHTSLNGMTPEAFAQHATKAYNNTQTLTQN
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TWAIREKEYSQRRACRLIGMDPKTWRYASRRPDDAAARGRLRELAGERRRFGYRRLHIL
CCCCCCHHHHHHHHHEEECCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEE
LGREGMTMNHKKLFRLYREEGLSVRKRGGRKRALGTRSPMMLPDGPNQRWSLDFVSDALN
ECCCCCCCCHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHC
NGRRFRVLTVVDDYTRECLALVADTSLSGERLGRELDRIGEHRGWPLMIVSDNGTEMTSN
CCCEEEEEEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCEECCC
AILAWQQKRSVLWHYIAPGKPQQNGFVESFNGRFRDECLNEHLFRNIAHARTVIEDWRAD
HHEEEHHHCCEEEEEECCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
YNAVRPHTSLNGMTPEAFAQHATKAYNNTQTLTQN
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1718819 [H]