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The map label for this gene is yeiT [H]

Identifier: 162147297

GI number: 162147297

Start: 1510729

End: 1512051

Strand: Direct

Name: yeiT [H]

Synonym: GDI_1502

Alternate gene names: 162147297

Gene position: 1510729-1512051 (Clockwise)

Preceding gene: 162147296

Following gene: 162147298

Centisome position: 38.3

GC content: 70.45

Gene sequence:

>1323_bases
ATGGCCCCGGACATCGCCGCAAACCGCCTTTCACCGGACGTGCTGGCCCGCAATTTCGCCGACGCGCACCCGCCGCTGGG
CGACCGGCAGGCGGTCATCGAGGCGGAGCGGTGCTACGCGTGCTATGACGCGCCCTGTATCGAGGCCTGCCCGACCGGCA
TCGACATTCCCGGCTTCATCAGGAAGATCGCGACGGGCAACCTCGCCGGGTCGGCCCGCACCATCCTGGAATCGAACATT
CTCGGCGGGTCCTGCGCGCGGGTCTGCCCCACCGAAATCCTGTGCGAACAGGCATGCGTGCGCAATACGCTGGACCAGAA
GCCCCTTTCCATCGGTGCCTTGCAGCGCCACGCGACCGACTGGCAGATGCGCCAGGGAAGGCAGCCCTTCGTCCGCGCGG
CCGCGACAGGCCGGCGCATCGCCGTGGTGGGGGCGGGGCCGGCCGGACTGGCCTGCGCGCACCGCCTGGCCCTGCATGGA
AACGACGTCACGGTGTTCGATGCCCGGCCCCGGGCGGGCGGCCTGAACGAATACGGCATCGCGGCCTACAAGCTGCCCGA
CGATTTCGCGCAGCGGGAAATCGACTTCCTGATCGCCATCGGCGGAATCACCATCGAGACAGGCCGCGCGCTGGGGCGCG
ATTTTACCCTGGCGTCGCTGGGGGCGGCGTATGACGCCATCTTCCTCGGCATCGGGCTGGCGGGGGTGCGTGCGCTGGGA
ATCGCGGGCGAACAGCTCGGCGGCGTGCTGAACGCGGTGGATTTCATCGAAACCCTGCGCGCGTCTTCCGGCAAGGACGA
CGTGCCGGTCGGGCGCCGGGTGGTGGTGATCGGTGGCGGGAATACCGCCGTCGATGCCGCCGTGCAGGCCAGGCGCCTGG
GCGCGGAAGAGGTCACGCTGGTCTATCGTCGCGGTGTCGCGCAGATGTCGGCGACAGATGTCGAACGGGAATGGGCGCAG
ACGAACGGCGTTTCCATCCGCTGCCGGGCCGCGCCCGCGCGGTTTCACGGCGTGGACGGCGCCGTCACCGCGATCACGTT
CGTCCAGGACACGCCGGACCACGACCGGGGCGAATTCACCCTGGCCGCCGACATGGTCCTGAAGGCCGTGGGCCAGCTTT
TCCCGCCGGACCCGCTGGACGGCGCGCTGGCGGTCGAGGACGGGCGGATCCGGGTGGACGCCCACGGCCGCACCTCGCGC
GCGGGGGTCTATGCCGGGGGCGACTGCACGAAGGGCGAGGATCTGACGGTCGCCGCCGTCCGTGACGGGCGGGACGCCGC
CGAAACCATTCATGCCGACCTGGCCAGCGCGACGGGGATCTGA

Upstream 100 bases:

>100_bases
ACCTGCTGACCGCCACGGGGCGGACCGGATCGAAGTTTTTTGGTTCTTTTTTTCGAAAAAGACCCAAGACTGTCGTCCCG
TATGGTGGAGAGTTCTCGTC

Downstream 100 bases:

>100_bases
GACATGGCCGACCTGCGCAGCACGCTGGCGGGCATCCGCTCGCCCAACCCGTTCTGGCTGGCCTCGGCGCCTCCGACCGA
CAAGGAATACAATGTCCGCC

Product: oxidoreductase

Products: L-glutamine; 2-oxoglutarate; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 440; Mature: 439

Protein sequence:

>440_residues
MAPDIAANRLSPDVLARNFADAHPPLGDRQAVIEAERCYACYDAPCIEACPTGIDIPGFIRKIATGNLAGSARTILESNI
LGGSCARVCPTEILCEQACVRNTLDQKPLSIGALQRHATDWQMRQGRQPFVRAAATGRRIAVVGAGPAGLACAHRLALHG
NDVTVFDARPRAGGLNEYGIAAYKLPDDFAQREIDFLIAIGGITIETGRALGRDFTLASLGAAYDAIFLGIGLAGVRALG
IAGEQLGGVLNAVDFIETLRASSGKDDVPVGRRVVVIGGGNTAVDAAVQARRLGAEEVTLVYRRGVAQMSATDVEREWAQ
TNGVSIRCRAAPARFHGVDGAVTAITFVQDTPDHDRGEFTLAADMVLKAVGQLFPPDPLDGALAVEDGRIRVDAHGRTSR
AGVYAGGDCTKGEDLTVAAVRDGRDAAETIHADLASATGI

Sequences:

>Translated_440_residues
MAPDIAANRLSPDVLARNFADAHPPLGDRQAVIEAERCYACYDAPCIEACPTGIDIPGFIRKIATGNLAGSARTILESNI
LGGSCARVCPTEILCEQACVRNTLDQKPLSIGALQRHATDWQMRQGRQPFVRAAATGRRIAVVGAGPAGLACAHRLALHG
NDVTVFDARPRAGGLNEYGIAAYKLPDDFAQREIDFLIAIGGITIETGRALGRDFTLASLGAAYDAIFLGIGLAGVRALG
IAGEQLGGVLNAVDFIETLRASSGKDDVPVGRRVVVIGGGNTAVDAAVQARRLGAEEVTLVYRRGVAQMSATDVEREWAQ
TNGVSIRCRAAPARFHGVDGAVTAITFVQDTPDHDRGEFTLAADMVLKAVGQLFPPDPLDGALAVEDGRIRVDAHGRTSR
AGVYAGGDCTKGEDLTVAAVRDGRDAAETIHADLASATGI
>Mature_439_residues
APDIAANRLSPDVLARNFADAHPPLGDRQAVIEAERCYACYDAPCIEACPTGIDIPGFIRKIATGNLAGSARTILESNIL
GGSCARVCPTEILCEQACVRNTLDQKPLSIGALQRHATDWQMRQGRQPFVRAAATGRRIAVVGAGPAGLACAHRLALHGN
DVTVFDARPRAGGLNEYGIAAYKLPDDFAQREIDFLIAIGGITIETGRALGRDFTLASLGAAYDAIFLGIGLAGVRALGI
AGEQLGGVLNAVDFIETLRASSGKDDVPVGRRVVVIGGGNTAVDAAVQARRLGAEEVTLVYRRGVAQMSATDVEREWAQT
NGVSIRCRAAPARFHGVDGAVTAITFVQDTPDHDRGEFTLAADMVLKAVGQLFPPDPLDGALAVEDGRIRVDAHGRTSRA
GVYAGGDCTKGEDLTVAAVRDGRDAAETIHADLASATGI

Specific function: Unknown

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To E.coli glutamate synthase [NADPH] small chain (GltD) and to E.coli AegA [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=467, Percent_Identity=33.6188436830835, Blast_Score=207, Evalue=2e-53,
Organism=Homo sapiens, GI237757300, Length=119, Percent_Identity=43.6974789915966, Blast_Score=96, Evalue=5e-20,
Organism=Escherichia coli, GI1788468, Length=425, Percent_Identity=35.5294117647059, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI1789606, Length=446, Percent_Identity=31.6143497757848, Blast_Score=199, Evalue=3e-52,
Organism=Escherichia coli, GI1788811, Length=463, Percent_Identity=32.829373650108, Blast_Score=197, Evalue=2e-51,
Organism=Escherichia coli, GI87082180, Length=456, Percent_Identity=31.7982456140351, Blast_Score=189, Evalue=3e-49,
Organism=Escherichia coli, GI1789243, Length=437, Percent_Identity=31.1212814645309, Blast_Score=151, Evalue=9e-38,
Organism=Caenorhabditis elegans, GI71984108, Length=463, Percent_Identity=34.1252699784017, Blast_Score=201, Evalue=5e-52,
Organism=Caenorhabditis elegans, GI17570289, Length=456, Percent_Identity=25.6578947368421, Blast_Score=127, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6320030, Length=472, Percent_Identity=26.9067796610169, Blast_Score=129, Evalue=7e-31,
Organism=Drosophila melanogaster, GI24640763, Length=452, Percent_Identity=35.8407079646018, Blast_Score=212, Evalue=3e-55,
Organism=Drosophila melanogaster, GI18858217, Length=452, Percent_Identity=35.8407079646018, Blast_Score=212, Evalue=3e-55,
Organism=Drosophila melanogaster, GI28574881, Length=441, Percent_Identity=29.2517006802721, Blast_Score=163, Evalue=2e-40,
Organism=Drosophila melanogaster, GI24665539, Length=441, Percent_Identity=29.2517006802721, Blast_Score=163, Evalue=2e-40,
Organism=Drosophila melanogaster, GI24665547, Length=442, Percent_Identity=29.185520361991, Blast_Score=162, Evalue=3e-40,
Organism=Drosophila melanogaster, GI24665543, Length=442, Percent_Identity=29.185520361991, Blast_Score=162, Evalue=3e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.4.1.13

Molecular weight: Translated: 46068; Mature: 45936

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPDIAANRLSPDVLARNFADAHPPLGDRQAVIEAERCYACYDAPCIEACPTGIDIPGFI
CCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHEECCCCHHHHCCCCCCCHHHH
RKIATGNLAGSARTILESNILGGSCARVCPTEILCEQACVRNTLDQKPLSIGALQRHATD
HHHHCCCCCCHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCC
WQMRQGRQPFVRAAATGRRIAVVGAGPAGLACAHRLALHGNDVTVFDARPRAGGLNEYGI
HHHHCCCCHHHHHHCCCCEEEEEECCCHHHHHHHHHEEECCEEEEEECCCCCCCCCCCCE
AAYKLPDDFAQREIDFLIAIGGITIETGRALGRDFTLASLGAAYDAIFLGIGLAGVRALG
EEEECCHHHHHHCEEEEEEECCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
IAGEQLGGVLNAVDFIETLRASSGKDDVPVGRRVVVIGGGNTAVDAAVQARRLGAEEVTL
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCHHHHHH
VYRRGVAQMSATDVEREWAQTNGVSIRCRAAPARFHGVDGAVTAITFVQDTPDHDRGEFT
HHHHCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCEEEEEEEECCCCCCCCCEE
LAADMVLKAVGQLFPPDPLDGALAVEDGRIRVDAHGRTSRAGVYAGGDCTKGEDLTVAAV
HHHHHHHHHHHHCCCCCCCCCEEEEECCEEEEEECCCCCCCCEEECCCCCCCCCEEEEEE
RDGRDAAETIHADLASATGI
CCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
APDIAANRLSPDVLARNFADAHPPLGDRQAVIEAERCYACYDAPCIEACPTGIDIPGFI
CCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHEECCCCHHHHCCCCCCCHHHH
RKIATGNLAGSARTILESNILGGSCARVCPTEILCEQACVRNTLDQKPLSIGALQRHATD
HHHHCCCCCCHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCC
WQMRQGRQPFVRAAATGRRIAVVGAGPAGLACAHRLALHGNDVTVFDARPRAGGLNEYGI
HHHHCCCCHHHHHHCCCCEEEEEECCCHHHHHHHHHEEECCEEEEEECCCCCCCCCCCCE
AAYKLPDDFAQREIDFLIAIGGITIETGRALGRDFTLASLGAAYDAIFLGIGLAGVRALG
EEEECCHHHHHHCEEEEEEECCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
IAGEQLGGVLNAVDFIETLRASSGKDDVPVGRRVVVIGGGNTAVDAAVQARRLGAEEVTL
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCHHHHHH
VYRRGVAQMSATDVEREWAQTNGVSIRCRAAPARFHGVDGAVTAITFVQDTPDHDRGEFT
HHHHCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCEEEEEEEECCCCCCCCCEE
LAADMVLKAVGQLFPPDPLDGALAVEDGRIRVDAHGRTSRAGVYAGGDCTKGEDLTVAAV
HHHHHHHHHHHHCCCCCCCCCEEEEECCEEEEEECCCCCCCCEEECCCCCCCCCEEEEEE
RDGRDAAETIHADLASATGI
CCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-glutamate; NADP+

Specific reaction: 2 L-glutamate + NADP+ = L-glutamine + 2-oxoglutarate + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]