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The map label for this gene is argF [H]

Identifier: 162147258

GI number: 162147258

Start: 1466897

End: 1467892

Strand: Reverse

Name: argF [H]

Synonym: GDI_1463

Alternate gene names: 162147258

Gene position: 1467892-1466897 (Counterclockwise)

Preceding gene: 162147259

Following gene: 162147257

Centisome position: 37.22

GC content: 70.68

Gene sequence:

>996_bases
ATGAACGCGCTGCCCACCACCACCCGGAACGCCGCCCCGCCCCGGCACTTCCTGGACCTGCGCGATTTCGACGGCGCGAC
GCTGCGCGCCATCCTGGACCTGGGCAGCCGCATCAAGCGGATGCAGCACGGGCGAACCCGCCCGCTGCACCCCCGCCAGC
CGCTGCTGGGCCGCGCGCTGGGGCTGATGCTGTCCAAGCCCTCGACCCGCACCCGCGTGTCGTTCGAGGTCGGAATGCGC
CAGTTGGGCGGCGACGTCATCCTGCTGTCGCCCAACGACATGCAACTGGGACGGGGCGAGAGCGTGGCCGACACCGCGCG
CGTGCTGTCGCGCTTCGTCGATGCCATCGTGCTGCGCACCGGCAATACCGCAGCCCTGCACGATCTGGCGCGCTGGGCCA
GCGTGCCGGTCATCAACGGGCTGACGCCGGACTCCCACCCGGTGCAGATCCTGGCCGACATCATGACGTTCGAGGAACAT
CGCGGCCCGATCGCCGGCCGCACGGTGGCGTGGGTGGGGGACGGCAACAATGTCGCGACCTCGTTCATCGAGGCGGCGTG
CCTGTTCGGCTTCCGCCTGCGCCTGGCCACCCCGCCCGGGCACACGCCTTCGGTCCAGGCCCTGGCCTGGGCACGGCAGA
GGGGCGCCGACATCGTCGTGACGACCGACCCGCGCGCCGCCGTCGCCGGGGCGGACTGCGTCGTCACCGACACCTGGGTC
AGCATGTCGGACACCGACGCGGCGCAGCGCCTGATCGCCTTCGACCCGTACCGGGTCGATGCCGCGCTGATGGCGCTGGC
GGCACCCGACGCCCTGTTCATGCACTGCCTGCCCGCCCATATCGGTGACGAAGTGACCGAGGACGTGTTCGAAGGCCCCC
ATTCGGTGGTGTTCGACGAGGCCGAGAACCGGTTGCACGCGCAGAAGGGGCTGCTGGTGTGGGCAATGGGCGGCGAGCAC
TGGCAGGATTACGGACAGGAGGAACCGACCCGATGA

Upstream 100 bases:

>100_bases
TGATCACCGAGGCCGATTGTGCCGAGGCCGGCCGGCGGCTGCGCCACGCGGCGCTGCGGGTGCGGGCGCACCTGGCGGCG
ACGGCCCGGGAGAGTGTGGC

Downstream 100 bases:

>100_bases
CAGGACCGGCCGAAACCCCCGCCCATCCCACCACCACCGAGGACGTTCCCGCCGCCCCCGGCTGGGTCGAAGGGGTCGTC
GAGGCCGCGTTCGCCACCCT

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase [H]

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MNALPTTTRNAAPPRHFLDLRDFDGATLRAILDLGSRIKRMQHGRTRPLHPRQPLLGRALGLMLSKPSTRTRVSFEVGMR
QLGGDVILLSPNDMQLGRGESVADTARVLSRFVDAIVLRTGNTAALHDLARWASVPVINGLTPDSHPVQILADIMTFEEH
RGPIAGRTVAWVGDGNNVATSFIEAACLFGFRLRLATPPGHTPSVQALAWARQRGADIVVTTDPRAAVAGADCVVTDTWV
SMSDTDAAQRLIAFDPYRVDAALMALAAPDALFMHCLPAHIGDEVTEDVFEGPHSVVFDEAENRLHAQKGLLVWAMGGEH
WQDYGQEEPTR

Sequences:

>Translated_331_residues
MNALPTTTRNAAPPRHFLDLRDFDGATLRAILDLGSRIKRMQHGRTRPLHPRQPLLGRALGLMLSKPSTRTRVSFEVGMR
QLGGDVILLSPNDMQLGRGESVADTARVLSRFVDAIVLRTGNTAALHDLARWASVPVINGLTPDSHPVQILADIMTFEEH
RGPIAGRTVAWVGDGNNVATSFIEAACLFGFRLRLATPPGHTPSVQALAWARQRGADIVVTTDPRAAVAGADCVVTDTWV
SMSDTDAAQRLIAFDPYRVDAALMALAAPDALFMHCLPAHIGDEVTEDVFEGPHSVVFDEAENRLHAQKGLLVWAMGGEH
WQDYGQEEPTR
>Mature_331_residues
MNALPTTTRNAAPPRHFLDLRDFDGATLRAILDLGSRIKRMQHGRTRPLHPRQPLLGRALGLMLSKPSTRTRVSFEVGMR
QLGGDVILLSPNDMQLGRGESVADTARVLSRFVDAIVLRTGNTAALHDLARWASVPVINGLTPDSHPVQILADIMTFEEH
RGPIAGRTVAWVGDGNNVATSFIEAACLFGFRLRLATPPGHTPSVQALAWARQRGADIVVTTDPRAAVAGADCVVTDTWV
SMSDTDAAQRLIAFDPYRVDAALMALAAPDALFMHCLPAHIGDEVTEDVFEGPHSVVFDEAENRLHAQKGLLVWAMGGEH
WQDYGQEEPTR

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI38788445, Length=309, Percent_Identity=42.0711974110032, Blast_Score=234, Evalue=9e-62,
Organism=Homo sapiens, GI18105007, Length=311, Percent_Identity=27.6527331189711, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI1790703, Length=327, Percent_Identity=38.8379204892966, Blast_Score=214, Evalue=4e-57,
Organism=Escherichia coli, GI1786469, Length=333, Percent_Identity=39.039039039039, Blast_Score=214, Evalue=9e-57,
Organism=Escherichia coli, GI2367364, Length=311, Percent_Identity=27.3311897106109, Blast_Score=89, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI193204318, Length=180, Percent_Identity=32.7777777777778, Blast_Score=81, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6322373, Length=320, Percent_Identity=34.0625, Blast_Score=190, Evalue=3e-49,
Organism=Saccharomyces cerevisiae, GI6322331, Length=311, Percent_Identity=26.0450160771704, Blast_Score=94, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24642586, Length=314, Percent_Identity=25.4777070063694, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.3 [H]

Molecular weight: Translated: 36052; Mature: 36052

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNALPTTTRNAAPPRHFLDLRDFDGATLRAILDLGSRIKRMQHGRTRPLHPRQPLLGRAL
CCCCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
GLMLSKPSTRTRVSFEVGMRQLGGDVILLSPNDMQLGRGESVADTARVLSRFVDAIVLRT
HHHHCCCCCCEEEEHHHHHHHHCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
GNTAALHDLARWASVPVINGLTPDSHPVQILADIMTFEEHRGPIAGRTVAWVGDGNNVAT
CCHHHHHHHHHHHCCCEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHH
SFIEAACLFGFRLRLATPPGHTPSVQALAWARQRGADIVVTTDPRAAVAGADCVVTDTWV
HHHHHHHHHHEEEEEECCCCCCCCHHHHHHHHHCCCEEEEECCCCHHHCCCCEEEEECCC
SMSDTDAAQRLIAFDPYRVDAALMALAAPDALFMHCLPAHIGDEVTEDVFEGPHSVVFDE
CCCCCHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHCHHHHHHHHCCCCCEEEHH
AENRLHAQKGLLVWAMGGEHWQDYGQEEPTR
HHHHHHHHCCEEEEEECCHHHHHCCCCCCCC
>Mature Secondary Structure
MNALPTTTRNAAPPRHFLDLRDFDGATLRAILDLGSRIKRMQHGRTRPLHPRQPLLGRAL
CCCCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
GLMLSKPSTRTRVSFEVGMRQLGGDVILLSPNDMQLGRGESVADTARVLSRFVDAIVLRT
HHHHCCCCCCEEEEHHHHHHHHCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
GNTAALHDLARWASVPVINGLTPDSHPVQILADIMTFEEHRGPIAGRTVAWVGDGNNVAT
CCHHHHHHHHHHHCCCEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHH
SFIEAACLFGFRLRLATPPGHTPSVQALAWARQRGADIVVTTDPRAAVAGADCVVTDTWV
HHHHHHHHHHEEEEEECCCCCCCCHHHHHHHHHCCCEEEEECCCCHHHCCCCEEEEECCC
SMSDTDAAQRLIAFDPYRVDAALMALAAPDALFMHCLPAHIGDEVTEDVFEGPHSVVFDE
CCCCCHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHCHHHHHHHHCCCCCEEEHH
AENRLHAQKGLLVWAMGGEHWQDYGQEEPTR
HHHHHHHHCCEEEEEECCHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA