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The map label for this gene is oprM [H]

Identifier: 162147081

GI number: 162147081

Start: 1285549

End: 1287051

Strand: Direct

Name: oprM [H]

Synonym: GDI_1286

Alternate gene names: 162147081

Gene position: 1285549-1287051 (Clockwise)

Preceding gene: 162147080

Following gene: 162147082

Centisome position: 32.59

GC content: 69.26

Gene sequence:

>1503_bases
ATGAACGGGTTTTCTCTTCCGCGCCGCGCCGGGCTGCTGGTCCTGGCGGCGCTGTCGGGCTGCACGCTGATCCCCGACTA
CAAGCGGCCGGCGCTGCCGGTCGCGGCAAGCTGGCCGAAAGGCGAGGCCTATCATGCCGGCAGCACCGGGCAGATCGGGA
CGGCGGCGTCCGACCTGGGCTGGCGCGACTTCTTCGTCGATCCGCGCCTGCAGGCGCTGATCGCGCTGGCGGTGCGCGAC
AATCGCGACCTGCGTTCGGTGGCTTCGGCGGTCATCGAGGCCCAGGGCCAGTACCGGGTGCAGCATGCCTCGCTGTTCCC
GCAGATCGGCGGGACCGGGATGGCGCTCTACCAGGCGCCGTCGGGGAATGGGGGCCTGAGCTTCGCGCCGGGCCTGGATG
CGGGCAAACACGGCGAGTACTACAGCAACGGCCACGTCTTTAAATATTACTCGGGCGGCATCGGCTTCTCGTCCTACGAG
ATCGATCTGTTCGGCCGCATCCGCAGCCTGTCGCGCGAGGCATCGGACCAAGTGCTCAGCCAGGCCGCCAACGCGCGCGG
GCTGCTGATCAGCGTGATCTCGCAGGTTGCGACGACCTATGTCGCGTGGCTGGCGGACCGCGAGCAGTTGCAGGTGACCG
AACGTACCCTGGCGTCCCAGCAGGATACGCTGCGCCTGACCCAGGCGCGGTTCGACCGGGGCGAGACCGACCAGCTGACC
CTGCGCCAGGTGGAAACCCAGGTCGAGCAGGCAGCCGCCTATCGTGCCCAGTACCTGCGCCAGATCGCGCAGGATGAAAA
CGAACTGGTTATGCTGGTGGGCGCGCCGCTGCCGGCCAACCTGCCGGACCCGGCCCCCTTCGGCCGCCAGACGGTGCTGG
CCGACCTGCCGGCGGGGCTGCCGTCCGACCTGCTGGACCGGCGGCCGGATGTCGTGTCGGCGGAATTCGCGCTGCTGTCG
GCCAACGACGATATCGGCGCCGCGCGCGCCGCCTTCTTCCCGCGCCTGATCCTGACGGCGTCGGACGGGGTGTCCAGCCT
GCAGTTCCGCAACCTGTTCACCTCCGGCGCCACGACGTGGGGGGCCAACCCGCAATTGCAGATCCCGCTGCTGACCTGGG
GCCAGAACGAGGGATCGCTGCAGGTCTCCAAATCCCGGCGGGACCAGCGCCTGATGGCGTACGAAAAGGCGATCCAGACC
GCGTTCCACGAGGTCTCGGACGCCCTGGCGGCGCGGGCGACTTACCTGGACCAGTCGCGGCAGCTCGACGCGCTGGTGGG
GTCCTCGGCCGATGCGTACCGGCTGGCGCGGATGCGCTTCGACGCGGGGGTGGATTCCTACCTGAACACGCTCGAGGCGC
AGCGCGCGCTCTATACCGCGCAGCAGAACCTGATCGTAGTGCAGGAAGCGCGCTACCAGAACCTGATCACCGTCTATCGC
GCCCTGGGCGGCGGCTGGACCGAACGCACCATTCCGCCGGCGACGGCGGTCCGGTCGGGGTAG

Upstream 100 bases:

>100_bases
GTGCTGCGGCTGTTCCGGGTCAAGCGGGTGAAGGACCGTCCGGACCCCTACGCGCACCTGTCCCGTCCCGCCGGGCCGTC
CGGTCAGGAGAGTTGAGTCG

Downstream 100 bases:

>100_bases
GTCGCATCGCCGGTGCGGGCAGGGCGGGTCCTTCGGGACCCGCCTTTTTTATGGCCGTTTTCATGGCCATGTCCGTGCCG
CCGGGCTGGGTCCAGCCCTG

Product: outer membrane protein oprM

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 500; Mature: 500

Protein sequence:

>500_residues
MNGFSLPRRAGLLVLAALSGCTLIPDYKRPALPVAASWPKGEAYHAGSTGQIGTAASDLGWRDFFVDPRLQALIALAVRD
NRDLRSVASAVIEAQGQYRVQHASLFPQIGGTGMALYQAPSGNGGLSFAPGLDAGKHGEYYSNGHVFKYYSGGIGFSSYE
IDLFGRIRSLSREASDQVLSQAANARGLLISVISQVATTYVAWLADREQLQVTERTLASQQDTLRLTQARFDRGETDQLT
LRQVETQVEQAAAYRAQYLRQIAQDENELVMLVGAPLPANLPDPAPFGRQTVLADLPAGLPSDLLDRRPDVVSAEFALLS
ANDDIGAARAAFFPRLILTASDGVSSLQFRNLFTSGATTWGANPQLQIPLLTWGQNEGSLQVSKSRRDQRLMAYEKAIQT
AFHEVSDALAARATYLDQSRQLDALVGSSADAYRLARMRFDAGVDSYLNTLEAQRALYTAQQNLIVVQEARYQNLITVYR
ALGGGWTERTIPPATAVRSG

Sequences:

>Translated_500_residues
MNGFSLPRRAGLLVLAALSGCTLIPDYKRPALPVAASWPKGEAYHAGSTGQIGTAASDLGWRDFFVDPRLQALIALAVRD
NRDLRSVASAVIEAQGQYRVQHASLFPQIGGTGMALYQAPSGNGGLSFAPGLDAGKHGEYYSNGHVFKYYSGGIGFSSYE
IDLFGRIRSLSREASDQVLSQAANARGLLISVISQVATTYVAWLADREQLQVTERTLASQQDTLRLTQARFDRGETDQLT
LRQVETQVEQAAAYRAQYLRQIAQDENELVMLVGAPLPANLPDPAPFGRQTVLADLPAGLPSDLLDRRPDVVSAEFALLS
ANDDIGAARAAFFPRLILTASDGVSSLQFRNLFTSGATTWGANPQLQIPLLTWGQNEGSLQVSKSRRDQRLMAYEKAIQT
AFHEVSDALAARATYLDQSRQLDALVGSSADAYRLARMRFDAGVDSYLNTLEAQRALYTAQQNLIVVQEARYQNLITVYR
ALGGGWTERTIPPATAVRSG
>Mature_500_residues
MNGFSLPRRAGLLVLAALSGCTLIPDYKRPALPVAASWPKGEAYHAGSTGQIGTAASDLGWRDFFVDPRLQALIALAVRD
NRDLRSVASAVIEAQGQYRVQHASLFPQIGGTGMALYQAPSGNGGLSFAPGLDAGKHGEYYSNGHVFKYYSGGIGFSSYE
IDLFGRIRSLSREASDQVLSQAANARGLLISVISQVATTYVAWLADREQLQVTERTLASQQDTLRLTQARFDRGETDQLT
LRQVETQVEQAAAYRAQYLRQIAQDENELVMLVGAPLPANLPDPAPFGRQTVLADLPAGLPSDLLDRRPDVVSAEFALLS
ANDDIGAARAAFFPRLILTASDGVSSLQFRNLFTSGATTWGANPQLQIPLLTWGQNEGSLQVSKSRRDQRLMAYEKAIQT
AFHEVSDALAARATYLDQSRQLDALVGSSADAYRLARMRFDAGVDSYLNTLEAQRALYTAQQNLIVVQEARYQNLITVYR
ALGGGWTERTIPPATAVRSG

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=482, Percent_Identity=34.0248962655602, Blast_Score=253, Evalue=2e-68,
Organism=Escherichia coli, GI2367348, Length=479, Percent_Identity=24.6346555323591, Blast_Score=105, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 54305; Mature: 54305

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGFSLPRRAGLLVLAALSGCTLIPDYKRPALPVAASWPKGEAYHAGSTGQIGTAASDLG
CCCCCCCHHHHHHHEEHHCCCEECCCCCCCCCCEECCCCCCCCEECCCCCCCCCCHHCCC
WRDFFVDPRLQALIALAVRDNRDLRSVASAVIEAQGQYRVQHASLFPQIGGTGMALYQAP
CHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEHHHHCCCCCCCCEEEEECC
SGNGGLSFAPGLDAGKHGEYYSNGHVFKYYSGGIGFSSYEIDLFGRIRSLSREASDQVLS
CCCCCCEECCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEHHHHHHHHHHHHHHHHHHH
QAANARGLLISVISQVATTYVAWLADREQLQVTERTLASQQDTLRLTQARFDRGETDQLT
HHHCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHH
LRQVETQVEQAAAYRAQYLRQIAQDENELVMLVGAPLPANLPDPAPFGRQTVLADLPAGL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHCCCCC
PSDLLDRRPDVVSAEFALLSANDDIGAARAAFFPRLILTASDGVSSLQFRNLFTSGATTW
CHHHHHCCCCCEEEEEEEEECCCCCCHHHHHHHCEEEEEECCCCHHHHHHHHHHCCCCCC
GANPQLQIPLLTWGQNEGSLQVSKSRRDQRLMAYEKAIQTAFHEVSDALAARATYLDQSR
CCCCEEEEEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLDALVGSSADAYRLARMRFDAGVDSYLNTLEAQRALYTAQQNLIVVQEARYQNLITVYR
HHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHH
ALGGGWTERTIPPATAVRSG
HHCCCCCCCCCCCHHHCCCC
>Mature Secondary Structure
MNGFSLPRRAGLLVLAALSGCTLIPDYKRPALPVAASWPKGEAYHAGSTGQIGTAASDLG
CCCCCCCHHHHHHHEEHHCCCEECCCCCCCCCCEECCCCCCCCEECCCCCCCCCCHHCCC
WRDFFVDPRLQALIALAVRDNRDLRSVASAVIEAQGQYRVQHASLFPQIGGTGMALYQAP
CHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEHHHHCCCCCCCCEEEEECC
SGNGGLSFAPGLDAGKHGEYYSNGHVFKYYSGGIGFSSYEIDLFGRIRSLSREASDQVLS
CCCCCCEECCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEHHHHHHHHHHHHHHHHHHH
QAANARGLLISVISQVATTYVAWLADREQLQVTERTLASQQDTLRLTQARFDRGETDQLT
HHHCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHH
LRQVETQVEQAAAYRAQYLRQIAQDENELVMLVGAPLPANLPDPAPFGRQTVLADLPAGL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHCCCCC
PSDLLDRRPDVVSAEFALLSANDDIGAARAAFFPRLILTASDGVSSLQFRNLFTSGATTW
CHHHHHCCCCCEEEEEEEEECCCCCCHHHHHHHCEEEEEECCCCHHHHHHHHHHCCCCCC
GANPQLQIPLLTWGQNEGSLQVSKSRRDQRLMAYEKAIQTAFHEVSDALAARATYLDQSR
CCCCEEEEEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLDALVGSSADAYRLARMRFDAGVDSYLNTLEAQRALYTAQQNLIVVQEARYQNLITVYR
HHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHH
ALGGGWTERTIPPATAVRSG
HHCCCCCCCCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]