Click here to switch to the map view.

The map label for this gene is grpE [H]

Identifier: 162147058

GI number: 162147058

Start: 1259905

End: 1260729

Strand: Reverse

Name: grpE [H]

Synonym: GDI_1263

Alternate gene names: 162147058

Gene position: 1260729-1259905 (Counterclockwise)

Preceding gene: 162147062

Following gene: 162147057

Centisome position: 31.96

GC content: 69.33

Gene sequence:

>825_bases
ATGACCATCCATCCATGTTCCGGCCGGGACCGCGTCCCGTTCCACCCGCATGGCGGGGGAAACCCGCACAAACGTCATAT
CGGCGATGGCCGTGACGCCTTGTCCAACGGCGCGCGCTCACCTACTTCAAAGGGCATGAGCCACGATACAGACCCCACCC
CCGTCGCCGACTCTCCGAGCGGAGAGGCCGGCATGTCCCCTCCCGGCCCGTCGTCCGGCCACGACCAGGATACGGCCCAC
GCCGGGGCGAGTGATCCGGCCGGTGCCGGCCACCCGCGCATCCAGGAGCTGGAAGCCGCGCTGGAGGAAATGCGCGAGAA
ATGGCTGCGCTCGGAAGCCGAGATGCAGAACCTGCGCACGCGCACCAAGCGCGAGCTGGAGGACGCGCGGCAATATGCGA
CGCAGAAATTCGCCCGCGACGTGGTCGAGGCCGCCGAGAACCTGAAGCGCGCGCTGGCCAGCCTGCCGCACGCGACCGAA
GGCGAGGACAGGCTGATCGCCCGCATGCGCGAGGGGATCGAGAGCACCGAGCGTTCGTTCATGGGCATCCTGGAACGCCA
TGGCATTTCCGCCGCCGACCCGGCCGGCACGCCGTTCGACGCCAACCACCACCAGGCGATGGCCGAGCAGCATAGCGACG
AACACCCCCACGGCACCGTGATCCAGGCCTGGACGCCCGCCTGGACGCTGCATGGCCGCCTGCTGAAGCCCGCGATGGTC
GTCGTGTCCAAGGGCGCGGCGGCAGGGACCCAGCCGGCCGCCGCCCAGGCAACGGAATCCGGGACGTACAGCGACCCGAC
ATACGGCAACAAGGCCGACGGCTGA

Upstream 100 bases:

>100_bases
TCGTCGGCTCCATCGCGGCGTTTCTCGCTCCTTGCCTGGCCGGGGGCGGCCTTCGCCCCAGCATAGCGCCAGCCGGATCA
CGCTGTCATGGCCGCAGCCC

Downstream 100 bases:

>100_bases
CCCGGCGACCGCCGGAACGTGCCCGCCCGCCCCTGTTCCGCAGGATGGCCCCTTGAAACGAACGCCGGGGCTGAATACAT
GCGAAACAGGTTCGGCGATG

Product: chaperone binding

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MTIHPCSGRDRVPFHPHGGGNPHKRHIGDGRDALSNGARSPTSKGMSHDTDPTPVADSPSGEAGMSPPGPSSGHDQDTAH
AGASDPAGAGHPRIQELEAALEEMREKWLRSEAEMQNLRTRTKRELEDARQYATQKFARDVVEAAENLKRALASLPHATE
GEDRLIARMREGIESTERSFMGILERHGISAADPAGTPFDANHHQAMAEQHSDEHPHGTVIQAWTPAWTLHGRLLKPAMV
VVSKGAAAGTQPAAAQATESGTYSDPTYGNKADG

Sequences:

>Translated_274_residues
MTIHPCSGRDRVPFHPHGGGNPHKRHIGDGRDALSNGARSPTSKGMSHDTDPTPVADSPSGEAGMSPPGPSSGHDQDTAH
AGASDPAGAGHPRIQELEAALEEMREKWLRSEAEMQNLRTRTKRELEDARQYATQKFARDVVEAAENLKRALASLPHATE
GEDRLIARMREGIESTERSFMGILERHGISAADPAGTPFDANHHQAMAEQHSDEHPHGTVIQAWTPAWTLHGRLLKPAMV
VVSKGAAAGTQPAAAQATESGTYSDPTYGNKADG
>Mature_273_residues
TIHPCSGRDRVPFHPHGGGNPHKRHIGDGRDALSNGARSPTSKGMSHDTDPTPVADSPSGEAGMSPPGPSSGHDQDTAHA
GASDPAGAGHPRIQELEAALEEMREKWLRSEAEMQNLRTRTKRELEDARQYATQKFARDVVEAAENLKRALASLPHATEG
EDRLIARMREGIESTERSFMGILERHGISAADPAGTPFDANHHQAMAEQHSDEHPHGTVIQAWTPAWTLHGRLLKPAMVV
VSKGAAAGTQPAAAQATESGTYSDPTYGNKADG

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Homo sapiens, GI24308295, Length=167, Percent_Identity=31.7365269461078, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI40255109, Length=156, Percent_Identity=26.9230769230769, Blast_Score=69, Evalue=6e-12,
Organism=Escherichia coli, GI1788967, Length=159, Percent_Identity=34.5911949685535, Blast_Score=93, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17552458, Length=166, Percent_Identity=29.5180722891566, Blast_Score=75, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6324806, Length=159, Percent_Identity=33.3333333333333, Blast_Score=101, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24653432, Length=153, Percent_Identity=31.3725490196078, Blast_Score=94, Evalue=1e-19,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 29306; Mature: 29175

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIHPCSGRDRVPFHPHGGGNPHKRHIGDGRDALSNGARSPTSKGMSHDTDPTPVADSPS
CCCCCCCCCCCCCCCCCCCCCCCHHCCCCCHHHHHCCCCCCHHCCCCCCCCCCCCCCCCC
GEAGMSPPGPSSGHDQDTAHAGASDPAGAGHPRIQELEAALEEMREKWLRSEAEMQNLRT
CCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RTKRELEDARQYATQKFARDVVEAAENLKRALASLPHATEGEDRLIARMREGIESTERSF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
MGILERHGISAADPAGTPFDANHHQAMAEQHSDEHPHGTVIQAWTPAWTLHGRLLKPAMV
HHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHH
VVSKGAAAGTQPAAAQATESGTYSDPTYGNKADG
HHHCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TIHPCSGRDRVPFHPHGGGNPHKRHIGDGRDALSNGARSPTSKGMSHDTDPTPVADSPS
CCCCCCCCCCCCCCCCCCCCCCHHCCCCCHHHHHCCCCCCHHCCCCCCCCCCCCCCCCC
GEAGMSPPGPSSGHDQDTAHAGASDPAGAGHPRIQELEAALEEMREKWLRSEAEMQNLRT
CCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RTKRELEDARQYATQKFARDVVEAAENLKRALASLPHATEGEDRLIARMREGIESTERSF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
MGILERHGISAADPAGTPFDANHHQAMAEQHSDEHPHGTVIQAWTPAWTLHGRLLKPAMV
HHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHH
VVSKGAAAGTQPAAAQATESGTYSDPTYGNKADG
HHHCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA