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The map label for this gene is rho [H]

Identifier: 162147052

GI number: 162147052

Start: 1251981

End: 1253288

Strand: Direct

Name: rho [H]

Synonym: GDI_1257

Alternate gene names: 162147052

Gene position: 1251981-1253288 (Clockwise)

Preceding gene: 162147051

Following gene: 162147059

Centisome position: 31.74

GC content: 64.22

Gene sequence:

>1308_bases
ATGCATCTCGCCGAACTCAAGGCCAAAACCCCCGCGGACCTTCTGGCGTACGCCGAAAGCCTGCAGATCGAGAACGCGTC
GTCCCTGCGCAAGCAGGACATGATGTTCGCCATCCTCAAGACCCTCGCCGACAATGATCAGGCGATCCATGGCGAGGGAA
CGCTGGAAATCCTGCCCGACGGGTTCGGCTTCCTGCGCTCGCCCGAGGCCAATTACCTGCCCGGGCCGGACGATATCTAT
ATCTCGCCCAGCCAGGTGCGGCGCTTCGGCCTGCGCACCGGCGACACGGTCGAGGGCCAGATCCGCGCCCCGCGCGACGG
CGAGCGCTATTTCGCGCTGCTGAAGGTCAACACGATCAATTTCGAGCCGCCCGAGGCCGTCCGTCACCGGATCAATTTCG
ACAACCTGACGCCCCTCTACCCCGAGCGCCGGCTGAAGATGGAAGTCGAGGCCAACGCCGTGGGCGGCGAGCCCGAGAAG
GTCGAGAAGGGCAAGGGCGCCAAGTCCCAGCCCAAGGATTTCACCCCCCGCGTGATCGACCTGGTCTCGCCGATCGGCAT
GGGGCAGCGCGCGCTGATCGTCGCCCCGCCGCGCACCGGCAAGACCGTGATGCTGCAGAGCATCGCCTCGTCGATTTCCG
CCAACCACCCCGAAGTCTTCCTGATCGTCCTGCTGATCGACGAGCGCCCGGAAGAAGTCACCGACATGGCCCGCTCCGTC
CGGGGCGAGGTCGTGTCCTCGACCTTCGACGAGCCGGCGACCCGCCACGTCCAGGTGACGGAAATGGTGCTGGAGAAGGC
CAAGCGGCTGGTCGAGCATAAGCGCGACGTCGTCATCCTGCTGGATTCCATCACCCGCCTGGCCCGCGCCTACAACACCG
TGGTGCCGTCATCGGGCAAGGTGCTGACCGGCGGCGTGGATGCCAATGCCCTGCAACGCCCCAAGCGCTTCTTCGGCGCC
GCGCGCAATATCGAGGAAGGCGGATCGCTGACCATCATCGCCACCGCGCTGATCGATACCGGCAGCCGCATGGACGAGGT
GATTTTCGAGGAATTCAAGGGCACCGGCAACTCGGAACTCATCCTCGACCGCAAGCTGGCCGACAAGCGCACCTTCCCGG
CGATCGACATCACCAAGAGCGGCACCCGCAAGGAAGAATTGCTGGTCGAACGCTCCGAACTGTCGAAGATGTGGGTCCTG
CGCCGCATCCTGGCGCCGATGGGCACGATGGACGCGATGGACTTTCTGCTGGACAAGCTGAAATACAGCAAGACCAACCG
GGATTTCTTCGACGCGATGAATACCTGA

Upstream 100 bases:

>100_bases
GTCTGAATGGCACCCGGTCTTTCGGCAAGCGCCTCATGCGCCAAGTGCCTGGTCAACCTGTTCCCTCCCCCATGTGACCT
GCCCATTCAAGGCATCGTGA

Downstream 100 bases:

>100_bases
CAGAAGGGAGCGTGATCGGGCGGAAACGCCTGGTCACGTTCTTTTTTGAAAAAAAGAACCAAAAAACTTCTGATCTATTC
AGAAATTTTGTGCCGAGTAC

Product: transcription termination factor Rho

Products: NA

Alternate protein names: ATP-dependent helicase Rho [H]

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MHLAELKAKTPADLLAYAESLQIENASSLRKQDMMFAILKTLADNDQAIHGEGTLEILPDGFGFLRSPEANYLPGPDDIY
ISPSQVRRFGLRTGDTVEGQIRAPRDGERYFALLKVNTINFEPPEAVRHRINFDNLTPLYPERRLKMEVEANAVGGEPEK
VEKGKGAKSQPKDFTPRVIDLVSPIGMGQRALIVAPPRTGKTVMLQSIASSISANHPEVFLIVLLIDERPEEVTDMARSV
RGEVVSSTFDEPATRHVQVTEMVLEKAKRLVEHKRDVVILLDSITRLARAYNTVVPSSGKVLTGGVDANALQRPKRFFGA
ARNIEEGGSLTIIATALIDTGSRMDEVIFEEFKGTGNSELILDRKLADKRTFPAIDITKSGTRKEELLVERSELSKMWVL
RRILAPMGTMDAMDFLLDKLKYSKTNRDFFDAMNT

Sequences:

>Translated_435_residues
MHLAELKAKTPADLLAYAESLQIENASSLRKQDMMFAILKTLADNDQAIHGEGTLEILPDGFGFLRSPEANYLPGPDDIY
ISPSQVRRFGLRTGDTVEGQIRAPRDGERYFALLKVNTINFEPPEAVRHRINFDNLTPLYPERRLKMEVEANAVGGEPEK
VEKGKGAKSQPKDFTPRVIDLVSPIGMGQRALIVAPPRTGKTVMLQSIASSISANHPEVFLIVLLIDERPEEVTDMARSV
RGEVVSSTFDEPATRHVQVTEMVLEKAKRLVEHKRDVVILLDSITRLARAYNTVVPSSGKVLTGGVDANALQRPKRFFGA
ARNIEEGGSLTIIATALIDTGSRMDEVIFEEFKGTGNSELILDRKLADKRTFPAIDITKSGTRKEELLVERSELSKMWVL
RRILAPMGTMDAMDFLLDKLKYSKTNRDFFDAMNT
>Mature_435_residues
MHLAELKAKTPADLLAYAESLQIENASSLRKQDMMFAILKTLADNDQAIHGEGTLEILPDGFGFLRSPEANYLPGPDDIY
ISPSQVRRFGLRTGDTVEGQIRAPRDGERYFALLKVNTINFEPPEAVRHRINFDNLTPLYPERRLKMEVEANAVGGEPEK
VEKGKGAKSQPKDFTPRVIDLVSPIGMGQRALIVAPPRTGKTVMLQSIASSISANHPEVFLIVLLIDERPEEVTDMARSV
RGEVVSSTFDEPATRHVQVTEMVLEKAKRLVEHKRDVVILLDSITRLARAYNTVVPSSGKVLTGGVDANALQRPKRFFGA
ARNIEEGGSLTIIATALIDTGSRMDEVIFEEFKGTGNSELILDRKLADKRTFPAIDITKSGTRKEELLVERSELSKMWVL
RRILAPMGTMDAMDFLLDKLKYSKTNRDFFDAMNT

Specific function: Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template [H]

COG id: COG1158

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Rho family [H]

Homologues:

Organism=Escherichia coli, GI1790217, Length=433, Percent_Identity=66.2817551963048, Blast_Score=593, Evalue=1e-171,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR011112
- InterPro:   IPR011113
- InterPro:   IPR004665 [H]

Pfam domain/function: PF00006 ATP-synt_ab; PF07498 Rho_N; PF07497 Rho_RNA_bind [H]

EC number: NA

Molecular weight: Translated: 48435; Mature: 48435

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHLAELKAKTPADLLAYAESLQIENASSLRKQDMMFAILKTLADNDQAIHGEGTLEILPD
CCCHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEECCC
GFGFLRSPEANYLPGPDDIYISPSQVRRFGLRTGDTVEGQIRAPRDGERYFALLKVNTIN
CCCCCCCCCCCCCCCCCCEEECHHHHHHHCCCCCCCCCCEEECCCCCCEEEEEEEEEECC
FEPPEAVRHRINFDNLTPLYPERRLKMEVEANAVGGEPEKVEKGKGAKSQPKDFTPRVID
CCCHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHCCCCCCCCCCCCCCHHHH
LVSPIGMGQRALIVAPPRTGKTVMLQSIASSISANHPEVFLIVLLIDERPEEVTDMARSV
HHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHH
RGEVVSSTFDEPATRHVQVTEMVLEKAKRLVEHKRDVVILLDSITRLARAYNTVVPSSGK
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCCCCC
VLTGGVDANALQRPKRFFGAARNIEEGGSLTIIATALIDTGSRMDEVIFEEFKGTGNSEL
EEECCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHCCCCCCEE
ILDRKLADKRTFPAIDITKSGTRKEELLVERSELSKMWVLRRILAPMGTMDAMDFLLDKL
EEECHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
KYSKTNRDFFDAMNT
HHCCCCCHHHHHCCC
>Mature Secondary Structure
MHLAELKAKTPADLLAYAESLQIENASSLRKQDMMFAILKTLADNDQAIHGEGTLEILPD
CCCHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEECCC
GFGFLRSPEANYLPGPDDIYISPSQVRRFGLRTGDTVEGQIRAPRDGERYFALLKVNTIN
CCCCCCCCCCCCCCCCCCEEECHHHHHHHCCCCCCCCCCEEECCCCCCEEEEEEEEEECC
FEPPEAVRHRINFDNLTPLYPERRLKMEVEANAVGGEPEKVEKGKGAKSQPKDFTPRVID
CCCHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHCCCCCCCCCCCCCCHHHH
LVSPIGMGQRALIVAPPRTGKTVMLQSIASSISANHPEVFLIVLLIDERPEEVTDMARSV
HHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHH
RGEVVSSTFDEPATRHVQVTEMVLEKAKRLVEHKRDVVILLDSITRLARAYNTVVPSSGK
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCCCCC
VLTGGVDANALQRPKRFFGAARNIEEGGSLTIIATALIDTGSRMDEVIFEEFKGTGNSEL
EEECCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHCCCCCCEE
ILDRKLADKRTFPAIDITKSGTRKEELLVERSELSKMWVLRRILAPMGTMDAMDFLLDKL
EEECHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
KYSKTNRDFFDAMNT
HHCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8606169 [H]