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The map label for this gene is rutF [H]

Identifier: 162147039

GI number: 162147039

Start: 1239549

End: 1240166

Strand: Direct

Name: rutF [H]

Synonym: GDI_1244

Alternate gene names: 162147039

Gene position: 1239549-1240166 (Clockwise)

Preceding gene: 162147034

Following gene: 162147043

Centisome position: 31.43

GC content: 65.7

Gene sequence:

>618_bases
ATGATGGTGGTTTCCTCCTGTTTTCCCTTGCGCGATAATATGACAGAAGATGCGGCGTTGGTGTGTCGCCCCGTCGAAAC
GGACCAGACGGACACGGACGACGGCAGGAAGGGCATGGACATGACGGTGGATTCCCAGACCTATCGGGACGCGATGGCGC
GGCTGGGCGCGGCCGTGAACGTGGTGACGACCGGCACGCTGGACGATCCGGTGGGGTTCACCGCCTCCGCCGTCTGTTCC
GTCACCGACGCGCCCCCCACCCTGCTGGTCTGCATGAACCGTTCGGCCCGTGCGCGTACCGCCTTCCACACCGGCGGGGC
GATGTGCATCAACGTCCTTTCGGCCGGGCAGCGGGACATTTCCGGCACCTTCGCCGGGCCGATGGACATGTACGAACGCT
TCACCGTCGGCCACTGGACGACGATGACGAGCGGCGCTCCGGCTCTGGAAGAGGCGATGGCGTCGTTCGACTGCCGGATC
GACCGGATTGTCGAGGTCGGAACCCACAGCGTCATGTTCGGGGTGGTCGATGCCATCCGCCTGGGGGCGGCACTCGGCGG
GCTGGTCTATTTCAACCGCACCTATCACGTCCTGCCCCACGGCGGCCACGACCTCTGA

Upstream 100 bases:

>100_bases
CCTGTCCAGCGCATTCAGCGCCTTCGGCCGGTTCAGCGTCATCCGCCCCAGGCGGCCCTGGCGGGTGATCAGCAGCGTGT
CGGCGTCCATGGCGGCATCC

Downstream 100 bases:

>100_bases
CCGCCTGTCTGCCTATGCGCGCCGGTGACGGCCCGACGCGCGTTTACTGACGGACGCGGCTGCGCGCGTAGGCGGCCAGA
TAGGCCCCGGTACTCAGCAC

Product: flavin reductase

Products: NA

Alternate protein names: FMN reductase; NAD(P)H:flavin oxidoreductase [H]

Number of amino acids: Translated: 205; Mature: 205

Protein sequence:

>205_residues
MMVVSSCFPLRDNMTEDAALVCRPVETDQTDTDDGRKGMDMTVDSQTYRDAMARLGAAVNVVTTGTLDDPVGFTASAVCS
VTDAPPTLLVCMNRSARARTAFHTGGAMCINVLSAGQRDISGTFAGPMDMYERFTVGHWTTMTSGAPALEEAMASFDCRI
DRIVEVGTHSVMFGVVDAIRLGAALGGLVYFNRTYHVLPHGGHDL

Sequences:

>Translated_205_residues
MMVVSSCFPLRDNMTEDAALVCRPVETDQTDTDDGRKGMDMTVDSQTYRDAMARLGAAVNVVTTGTLDDPVGFTASAVCS
VTDAPPTLLVCMNRSARARTAFHTGGAMCINVLSAGQRDISGTFAGPMDMYERFTVGHWTTMTSGAPALEEAMASFDCRI
DRIVEVGTHSVMFGVVDAIRLGAALGGLVYFNRTYHVLPHGGHDL
>Mature_205_residues
MMVVSSCFPLRDNMTEDAALVCRPVETDQTDTDDGRKGMDMTVDSQTYRDAMARLGAAVNVVTTGTLDDPVGFTASAVCS
VTDAPPTLLVCMNRSARARTAFHTGGAMCINVLSAGQRDISGTFAGPMDMYERFTVGHWTTMTSGAPALEEAMASFDCRI
DRIVEVGTHSVMFGVVDAIRLGAALGGLVYFNRTYHVLPHGGHDL

Specific function: Catalyzes the reduction of FMN to FMNH2 which is used to reduce pyrimidine by RutA via the Rut pathway [H]

COG id: COG1853

COG function: function code R; Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the non-flavoprotein flavin reductase family. RutF subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081819, Length=156, Percent_Identity=52.5641025641026, Blast_Score=171, Evalue=2e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019917
- InterPro:   IPR002563
- InterPro:   IPR012349
- InterPro:   IPR009002 [H]

Pfam domain/function: PF01613 Flavin_Reduct [H]

EC number: =1.5.1.29 [H]

Molecular weight: Translated: 21922; Mature: 21922

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
6.3 %Met     (Translated Protein)
9.3 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
6.3 %Met     (Mature Protein)
9.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMVVSSCFPLRDNMTEDAALVCRPVETDQTDTDDGRKGMDMTVDSQTYRDAMARLGAAVN
CEEECCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCEEECHHHHHHHHHHHCCEEE
VVTTGTLDDPVGFTASAVCSVTDAPPTLLVCMNRSARARTAFHTGGAMCINVLSAGQRDI
EEEECCCCCCCCCCHHHEEEECCCCCEEEEEECCCCCHHEEECCCHHHHHHHHHCCCCCC
SGTFAGPMDMYERFTVGHWTTMTSGAPALEEAMASFDCRIDRIVEVGTHSVMFGVVDAIR
CCCCCCHHHHHHHHCCCCEEECCCCCHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHH
LGAALGGLVYFNRTYHVLPHGGHDL
HHHHHHHHEEECCEEEEECCCCCCC
>Mature Secondary Structure
MMVVSSCFPLRDNMTEDAALVCRPVETDQTDTDDGRKGMDMTVDSQTYRDAMARLGAAVN
CEEECCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCEEECHHHHHHHHHHHCCEEE
VVTTGTLDDPVGFTASAVCSVTDAPPTLLVCMNRSARARTAFHTGGAMCINVLSAGQRDI
EEEECCCCCCCCCCHHHEEEECCCCCEEEEEECCCCCHHEEECCCHHHHHHHHHCCCCCC
SGTFAGPMDMYERFTVGHWTTMTSGAPALEEAMASFDCRIDRIVEVGTHSVMFGVVDAIR
CCCCCCHHHHHHHHCCCCEEECCCCCHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHH
LGAALGGLVYFNRTYHVLPHGGHDL
HHHHHHHHEEECCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA