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The map label for this gene is fdhH [H]

Identifier: 162146939

GI number: 162146939

Start: 1136945

End: 1139221

Strand: Reverse

Name: fdhH [H]

Synonym: GDI_1144

Alternate gene names: 162146939

Gene position: 1139221-1136945 (Counterclockwise)

Preceding gene: 162146940

Following gene: 162146937

Centisome position: 28.88

GC content: 66.36

Gene sequence:

>2277_bases
ATGTCCGACGAGGTGAAATTCCGTCCCTACACCCATCCGGCCGGCGGCTGGGGGGCGGCGAAGGCTACGGCGCGGGTCCT
GCTGGAACAGAGCGTGGTCACCACCGGGTCGCGCGCGCTGCTGTCGATGAACCATCCCCACGGGTTCAAATGTCCCAGTT
GCGCCTGGCCCAATCCCGACCGGGAAAAGACGCTGGAATTCTGCGAAAACGGCGCCAAGGCCCTGGCCGTCGAGGCGACG
AAGCGGCGGATCGGGCGTGATTTCTTCGCCGCCCATACCGTCACCGAACTGATGGAACGCAGCGATTACTGGCTGGAGCA
GCAGGGACGGCTGACCGAGCCGATGCGCTACGACGCGGCGACCGACCGCTACGTGCCCTGCGACTGGGACGCCGCGTTCG
CGATGATCGGCGACGCGCTGCGGGCGCTGGACCATCCGGACCAGGCGGAATTCTATACGTCGGGCCGCACTTCGAACGAG
GCCGCGTTCCTGTATTCCGTATTCGTGCGGGAATTCGGGACCAACAATTTCCCCGACTGCTCCAACATGTGCCACGAGCC
GACCAGCCGGGGCCTGCCGGCATCGATCGGCATCGGCAAGGGCACGGTGATCATCAACGATTTCGCCCATACCGAGGCGA
TCTTCATCATCGGCCAGAATACCGGCACCAACAGCCCGCGCATGATGACCGAGCTGGTCCATGCGCGGAAGCGCGGCGTG
CCGATCGTCGTCATCAACCCGATGCCCGAACGCGCGCTGATGCGCTTCACCGAACCGCAGGACCCGGTGAAGATGGCGAC
GTTCGGCTCGACCGAGATTGCCAGCGAATTCTGCCAGGTCCGGATCGGCGGCGACATCGCCGTGATCAAGGGCATGATGA
AGACGCTGCTGGAACTGGATGACGCGGCCCGGGCGGCCGGCACGCCGCGCCTGCTGGACGTCGATTTCATCGCCGCCCAC
ACGGATGGCTTCGACGCGGTGGCGCAGGATGCGCGGGCCACAAGCTGGGCCGACATCGTCGCGGTGTCGGGGCTGGAGGA
AGCGCAGATCCGCCGCATCGGTGCGATCTATGCCCATGCGGGCGCCAGCATCGTCTGCTACGGCATGGGCATCACCCAGC
ACCAACAGGGCGCGCGGGCGATCCAGCAGATCGCCAATCTCCTGATGATGAAGGGGAATTTCGGCAGGGCCGGGGGCGGG
ATCTGCCCGGTGCGCGGCCATTCCAACGTCCAGGGCGACCGCACCGTGGGCATCGACGAAAAGCCGACCCCCGCCTACCT
GGCCCGGCTGCAGGCCGCCTTCGGCTTCACCCCGCCGAGCGAGCATGGACACCATGTCGTCGAATCGGTGAAGGCGATGA
TCGACGGGCGTGCGCGCGTCTTCATCGGCATGGGCGGCAATTTCATCCACGCCATCCCCGACACGCCCATCGCGTACCGG
GCGATGGCGACACTGGACCTGACGGTGGGCATCGCGACCAAGCTCAATCGCGGCCATCTGGTCCACGGGCGGCAGGCGCT
GATCCTGCCCGTCATCGCGCGGTCGGAGATCGACATCCAGGCCAGCGGCCCGCAATTCGTGACGCTGGAGGACGCGATGT
CCAATGTCGGCGCGTCACGCGGTGTGCTGGAGCCCGCCAGCCCGCATCTGCTGTCGGAAATCGAGATCGTCTGCCGGATG
GCCAAGGCGACTCTGCCGCACAGCGTCGTGCCGTGGGGCACCTATATCGACGATTACAACCTGATCCGCGACAAGATCGC
CGAGGTCTATCCCGATTTCGCGGACTTCAACGTCCGCATCCACGAACCCAAGGGGTTCCACCTGTACAATCCGCCGCGCC
ATCGGGAATGGCGGACGGCGACGGGCAAGGCCAACTTCCTGCCGTTCGCTGGGCTGGCGATGGACCCGCCGGTGGACGAC
GCCGCCATGCTGCGGCTGGCGACGGTGCGCTCGCACGACCAGTACAACACCACCATCTACAGCAATACCGACCGCTATCG
CGGGGTGTACGATACCCGCATGGTGGTGCTGATGAACGCGGACGACATGCGCGACCGCGCCATTGCCCCCGAATCCCCCG
TGGTGCTGGAAACCCTGTCGGATGACGGCATCGTGCGGCGGGTGGGCGACCTGCGCGCGCTGCCCTATTCCCTGCCGCGC
GGTGCGGTGGCGGGCTATTACCCCGAACTCAACCCCCTGCTGCCGCTGGACCATTTCGACGAGATCAGCGGCACCCCGGC
GGCGAAGTCGATTCCGGTGCGGGTGGTGGCGGCCTGA

Upstream 100 bases:

>100_bases
GCTGACCCTGGTTGCGCTGGCGCGGCACGACGCGCAGATTATCTTCACGCATCAAGGCCGGATCGACACGGCCGCCTGAT
CACGCAAGCGGGGGGCATCC

Downstream 100 bases:

>100_bases
CCGTCGCCGTCGCTGCCGGCGGGGGGACCATCGCATCCAGCAGCACGTGCGGATCGCCCCCGCAGGACGGGGCGGCCAGC
AGGGGATCGAGCCGCCGGTC

Product: formate dehydrogenase alpha subunit

Products: CO2; NADH

Alternate protein names: NA

Number of amino acids: Translated: 758; Mature: 757

Protein sequence:

>758_residues
MSDEVKFRPYTHPAGGWGAAKATARVLLEQSVVTTGSRALLSMNHPHGFKCPSCAWPNPDREKTLEFCENGAKALAVEAT
KRRIGRDFFAAHTVTELMERSDYWLEQQGRLTEPMRYDAATDRYVPCDWDAAFAMIGDALRALDHPDQAEFYTSGRTSNE
AAFLYSVFVREFGTNNFPDCSNMCHEPTSRGLPASIGIGKGTVIINDFAHTEAIFIIGQNTGTNSPRMMTELVHARKRGV
PIVVINPMPERALMRFTEPQDPVKMATFGSTEIASEFCQVRIGGDIAVIKGMMKTLLELDDAARAAGTPRLLDVDFIAAH
TDGFDAVAQDARATSWADIVAVSGLEEAQIRRIGAIYAHAGASIVCYGMGITQHQQGARAIQQIANLLMMKGNFGRAGGG
ICPVRGHSNVQGDRTVGIDEKPTPAYLARLQAAFGFTPPSEHGHHVVESVKAMIDGRARVFIGMGGNFIHAIPDTPIAYR
AMATLDLTVGIATKLNRGHLVHGRQALILPVIARSEIDIQASGPQFVTLEDAMSNVGASRGVLEPASPHLLSEIEIVCRM
AKATLPHSVVPWGTYIDDYNLIRDKIAEVYPDFADFNVRIHEPKGFHLYNPPRHREWRTATGKANFLPFAGLAMDPPVDD
AAMLRLATVRSHDQYNTTIYSNTDRYRGVYDTRMVVLMNADDMRDRAIAPESPVVLETLSDDGIVRRVGDLRALPYSLPR
GAVAGYYPELNPLLPLDHFDEISGTPAAKSIPVRVVAA

Sequences:

>Translated_758_residues
MSDEVKFRPYTHPAGGWGAAKATARVLLEQSVVTTGSRALLSMNHPHGFKCPSCAWPNPDREKTLEFCENGAKALAVEAT
KRRIGRDFFAAHTVTELMERSDYWLEQQGRLTEPMRYDAATDRYVPCDWDAAFAMIGDALRALDHPDQAEFYTSGRTSNE
AAFLYSVFVREFGTNNFPDCSNMCHEPTSRGLPASIGIGKGTVIINDFAHTEAIFIIGQNTGTNSPRMMTELVHARKRGV
PIVVINPMPERALMRFTEPQDPVKMATFGSTEIASEFCQVRIGGDIAVIKGMMKTLLELDDAARAAGTPRLLDVDFIAAH
TDGFDAVAQDARATSWADIVAVSGLEEAQIRRIGAIYAHAGASIVCYGMGITQHQQGARAIQQIANLLMMKGNFGRAGGG
ICPVRGHSNVQGDRTVGIDEKPTPAYLARLQAAFGFTPPSEHGHHVVESVKAMIDGRARVFIGMGGNFIHAIPDTPIAYR
AMATLDLTVGIATKLNRGHLVHGRQALILPVIARSEIDIQASGPQFVTLEDAMSNVGASRGVLEPASPHLLSEIEIVCRM
AKATLPHSVVPWGTYIDDYNLIRDKIAEVYPDFADFNVRIHEPKGFHLYNPPRHREWRTATGKANFLPFAGLAMDPPVDD
AAMLRLATVRSHDQYNTTIYSNTDRYRGVYDTRMVVLMNADDMRDRAIAPESPVVLETLSDDGIVRRVGDLRALPYSLPR
GAVAGYYPELNPLLPLDHFDEISGTPAAKSIPVRVVAA
>Mature_757_residues
SDEVKFRPYTHPAGGWGAAKATARVLLEQSVVTTGSRALLSMNHPHGFKCPSCAWPNPDREKTLEFCENGAKALAVEATK
RRIGRDFFAAHTVTELMERSDYWLEQQGRLTEPMRYDAATDRYVPCDWDAAFAMIGDALRALDHPDQAEFYTSGRTSNEA
AFLYSVFVREFGTNNFPDCSNMCHEPTSRGLPASIGIGKGTVIINDFAHTEAIFIIGQNTGTNSPRMMTELVHARKRGVP
IVVINPMPERALMRFTEPQDPVKMATFGSTEIASEFCQVRIGGDIAVIKGMMKTLLELDDAARAAGTPRLLDVDFIAAHT
DGFDAVAQDARATSWADIVAVSGLEEAQIRRIGAIYAHAGASIVCYGMGITQHQQGARAIQQIANLLMMKGNFGRAGGGI
CPVRGHSNVQGDRTVGIDEKPTPAYLARLQAAFGFTPPSEHGHHVVESVKAMIDGRARVFIGMGGNFIHAIPDTPIAYRA
MATLDLTVGIATKLNRGHLVHGRQALILPVIARSEIDIQASGPQFVTLEDAMSNVGASRGVLEPASPHLLSEIEIVCRMA
KATLPHSVVPWGTYIDDYNLIRDKIAEVYPDFADFNVRIHEPKGFHLYNPPRHREWRTATGKANFLPFAGLAMDPPVDDA
AMLRLATVRSHDQYNTTIYSNTDRYRGVYDTRMVVLMNADDMRDRAIAPESPVVLETLSDDGIVRRVGDLRALPYSLPRG
AVAGYYPELNPLLPLDHFDEISGTPAAKSIPVRVVAA

Specific function: Probably involved in acid resistance [H]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI1787778, Length=752, Percent_Identity=51.1968085106383, Blast_Score=780, Evalue=0.0,
Organism=Escherichia coli, GI3868721, Length=394, Percent_Identity=26.1421319796954, Blast_Score=144, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR010046 [H]

Pfam domain/function: PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: 1.2.1.2

Molecular weight: Translated: 82943; Mature: 82812

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDEVKFRPYTHPAGGWGAAKATARVLLEQSVVTTGSRALLSMNHPHGFKCPSCAWPNPD
CCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCC
REKTLEFCENGAKALAVEATKRRIGRDFFAAHTVTELMERSDYWLEQQGRLTEPMRYDAA
HHHHHHHHHCCCCEEEEEHHHHHHCHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCC
TDRYVPCDWDAAFAMIGDALRALDHPDQAEFYTSGRTSNEAAFLYSVFVREFGTNNFPDC
CCCCCCCCHHHHHHHHHHHHHHHCCCCCHHEEECCCCCCCHHHHHHHHHHHHCCCCCCCH
SNMCHEPTSRGLPASIGIGKGTVIINDFAHTEAIFIIGQNTGTNSPRMMTELVHARKRGV
HHHHCCCCCCCCCCCCCCCCCEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCC
PIVVINPMPERALMRFTEPQDPVKMATFGSTEIASEFCQVRIGGDIAVIKGMMKTLLELD
CEEEECCCCHHHHHHCCCCCCCEEEEECCCHHHHHHHHEEEECCCHHHHHHHHHHHHHHH
DAARAAGTPRLLDVDFIAAHTDGFDAVAQDARATSWADIVAVSGLEEAQIRRIGAIYAHA
HHHHHCCCCCEEEEEEEEECCCCHHHHHHHCCCCCHHHHHEECCCCHHHHHHHHHHEECC
GASIVCYGMGITQHQQGARAIQQIANLLMMKGNFGRAGGGICPVRGHSNVQGDRTVGIDE
CCEEEEEECCCCHHHHHHHHHHHHHHHHEECCCCCCCCCCEEEECCCCCCCCCEEECCCC
KPTPAYLARLQAAFGFTPPSEHGHHVVESVKAMIDGRARVFIGMGGNFIHAIPDTPIAYR
CCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCEEEECCCCCHHHH
AMATLDLTVGIATKLNRGHLVHGRQALILPVIARSEIDIQASGPQFVTLEDAMSNVGASR
EEEEEEEEEEEEEECCCCCEECCCCEEEEEEEECCCEEEEECCCCEEEHHHHHHHCCCCC
GVLEPASPHLLSEIEIVCRMAKATLPHSVVPWGTYIDDYNLIRDKIAEVYPDFADFNVRI
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEE
HEPKGFHLYNPPRHREWRTATGKANFLPFAGLAMDPPVDDAAMLRLATVRSHDQYNTTIY
ECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEE
SNTDRYRGVYDTRMVVLMNADDMRDRAIAPESPVVLETLSDDGIVRRVGDLRALPYSLPR
CCCCCCCCEECCEEEEEECCCCCHHCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCC
GAVAGYYPELNPLLPLDHFDEISGTPAAKSIPVRVVAA
CCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCEEEEEC
>Mature Secondary Structure 
SDEVKFRPYTHPAGGWGAAKATARVLLEQSVVTTGSRALLSMNHPHGFKCPSCAWPNPD
CCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCC
REKTLEFCENGAKALAVEATKRRIGRDFFAAHTVTELMERSDYWLEQQGRLTEPMRYDAA
HHHHHHHHHCCCCEEEEEHHHHHHCHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCC
TDRYVPCDWDAAFAMIGDALRALDHPDQAEFYTSGRTSNEAAFLYSVFVREFGTNNFPDC
CCCCCCCCHHHHHHHHHHHHHHHCCCCCHHEEECCCCCCCHHHHHHHHHHHHCCCCCCCH
SNMCHEPTSRGLPASIGIGKGTVIINDFAHTEAIFIIGQNTGTNSPRMMTELVHARKRGV
HHHHCCCCCCCCCCCCCCCCCEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCC
PIVVINPMPERALMRFTEPQDPVKMATFGSTEIASEFCQVRIGGDIAVIKGMMKTLLELD
CEEEECCCCHHHHHHCCCCCCCEEEEECCCHHHHHHHHEEEECCCHHHHHHHHHHHHHHH
DAARAAGTPRLLDVDFIAAHTDGFDAVAQDARATSWADIVAVSGLEEAQIRRIGAIYAHA
HHHHHCCCCCEEEEEEEEECCCCHHHHHHHCCCCCHHHHHEECCCCHHHHHHHHHHEECC
GASIVCYGMGITQHQQGARAIQQIANLLMMKGNFGRAGGGICPVRGHSNVQGDRTVGIDE
CCEEEEEECCCCHHHHHHHHHHHHHHHHEECCCCCCCCCCEEEECCCCCCCCCEEECCCC
KPTPAYLARLQAAFGFTPPSEHGHHVVESVKAMIDGRARVFIGMGGNFIHAIPDTPIAYR
CCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCEEEECCCCCHHHH
AMATLDLTVGIATKLNRGHLVHGRQALILPVIARSEIDIQASGPQFVTLEDAMSNVGASR
EEEEEEEEEEEEEECCCCCEECCCCEEEEEEEECCCEEEEECCCCEEEHHHHHHHCCCCC
GVLEPASPHLLSEIEIVCRMAKATLPHSVVPWGTYIDDYNLIRDKIAEVYPDFADFNVRI
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEE
HEPKGFHLYNPPRHREWRTATGKANFLPFAGLAMDPPVDDAAMLRLATVRSHDQYNTTIY
ECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEE
SNTDRYRGVYDTRMVVLMNADDMRDRAIAPESPVVLETLSDDGIVRRVGDLRALPYSLPR
CCCCCCCCEECCEEEEEECCCCCHHCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCC
GAVAGYYPELNPLLPLDHFDEISGTPAAKSIPVRVVAA
CCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]