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The map label for this gene is relE [H]

Identifier: 162146884

GI number: 162146884

Start: 1073705

End: 1074049

Strand: Direct

Name: relE [H]

Synonym: GDI_1072

Alternate gene names: 162146884

Gene position: 1073705-1074049 (Clockwise)

Preceding gene: 162146883

Following gene: 162146885

Centisome position: 27.22

GC content: 57.97

Gene sequence:

>345_bases
ATGACTGAATACGCGATCGAATTCGATGATCGCGCACTTCGGGAATGGGACGGGCTTGACGGCAGCATTCGCAGGAAGTT
CGAGAAAAAGCTCGAAAAACTGGTTCTGAACCCGCATTCTCCCGGTAACGAACTGCACGGCGATCTCGCGGGATTCTACA
AGATCAAGCTGCGGCAGGACGGCTATCGCCTTGTCTATCAGGTCGTGGAACAGCGGATCGTCATCTTCGTCATCGCGGTC
GGACGCCGCGAGGACAACGAAATCTACACGGCGGCAACCGGGCGCATCCCGGAGACGCCAGCCGATCGGACACGTCAGCC
CAGCAGCAGAAGCCGCAAGAGGTAA

Upstream 100 bases:

>100_bases
GACGAAGATGCAAGGCTGATCGAAATCGTCCGAGAACGCCTCGCCGATCCCGCCCCCCGCCACCGCATGACGCTGGCGGA
ACTCAAGGCGCGCCATCCGG

Downstream 100 bases:

>100_bases
ACCTTACAAAACCACCTGTCGGGAGATTCACCATGACCCGCGTTGCTCTCTATGCCCGCTATTCCTCGGACAACCAGCGG
GCGGCTTCGATCGAGGACCA

Product: relE protein (antitoxin)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 114; Mature: 113

Protein sequence:

>114_residues
MTEYAIEFDDRALREWDGLDGSIRRKFEKKLEKLVLNPHSPGNELHGDLAGFYKIKLRQDGYRLVYQVVEQRIVIFVIAV
GRREDNEIYTAATGRIPETPADRTRQPSSRSRKR

Sequences:

>Translated_114_residues
MTEYAIEFDDRALREWDGLDGSIRRKFEKKLEKLVLNPHSPGNELHGDLAGFYKIKLRQDGYRLVYQVVEQRIVIFVIAV
GRREDNEIYTAATGRIPETPADRTRQPSSRSRKR
>Mature_113_residues
TEYAIEFDDRALREWDGLDGSIRRKFEKKLEKLVLNPHSPGNELHGDLAGFYKIKLRQDGYRLVYQVVEQRIVIFVIAVG
RREDNEIYTAATGRIPETPADRTRQPSSRSRKR

Specific function: Inhibits translation during amino acid starvation. Acts by cleaving mRNA with high codon specificity in the ribosomal A site. UGA is cleaved with fast rate while UAA and UGA are cleaved with intermediate and slow rate, respectively. mRNA cleavage can also

COG id: COG2026

COG function: function code JD; Cytotoxic translational repressor of toxin-antitoxin stability system

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787846, Length=93, Percent_Identity=47.3118279569892, Blast_Score=96, Evalue=5e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007712
- InterPro:   IPR012753 [H]

Pfam domain/function: PF05016 Plasmid_stabil [H]

EC number: NA

Molecular weight: Translated: 13319; Mature: 13188

Theoretical pI: Translated: 9.96; Mature: 9.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEYAIEFDDRALREWDGLDGSIRRKFEKKLEKLVLNPHSPGNELHGDLAGFYKIKLRQD
CCCEEEECCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCC
GYRLVYQVVEQRIVIFVIAVGRREDNEIYTAATGRIPETPADRTRQPSSRSRKR
CHHHHHHHHHHHHEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TEYAIEFDDRALREWDGLDGSIRRKFEKKLEKLVLNPHSPGNELHGDLAGFYKIKLRQD
CCEEEECCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCC
GYRLVYQVVEQRIVIFVIAVGRREDNEIYTAATGRIPETPADRTRQPSSRSRKR
CHHHHHHHHHHHHEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2990907; 9097039; 9278503; 9767574; 12526800 [H]