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The map label for this gene is pucI [H]

Identifier: 162146864

GI number: 162146864

Start: 1060846

End: 1062321

Strand: Reverse

Name: pucI [H]

Synonym: GDI_1047

Alternate gene names: 162146864

Gene position: 1062321-1060846 (Counterclockwise)

Preceding gene: 162146866

Following gene: 162146861

Centisome position: 26.93

GC content: 68.02

Gene sequence:

>1476_bases
ATGGCTCCAGCGCAGGGCCCAACCGGAAAAGATCCCATGCTGGACACGACCCAGACCGCCCGCGACCCCCTGCTGTGGAA
TCCCGACATCGCCCCCCTGCCCCGGCAGAAACGGGACTGGACATGGATCACCCTCGCCGCCGTCTGGATGGGCATGGTCC
ATAACGTGGTCGCCTACGAGGCCGCGGCCGGCCTCATGGCCATCGGCTTCTCCGCCATGCAGTGCCTGCTCATCGTCTGG
ACGGCCTATTGCTCGCTGTTCGTCGCCATGTGGTTCAATGCCCGCGCGGGCACCGAATGGGGCATTCCCTTCTGCGTCCT
GATCCGCGCCGCCTTCGGCCCGCGCGGCGCCGTCATCCCCGTCGCCATCCGCGCCGTGGTCGCCATCTTCTGGTTCTCGG
TCCAGTCCTATGCGGCCTCGCAGGCGCTCGACGCCATCGCCGCCAGCCTGTGGCCGGACTGGAGCAGCCTGCACCTTCCC
GACGGCACGGCGCTCGGCCCCTGGCTGGCGGTGACGATGATCTGGGGCCTGCACGCCCTCGTCGCCGCCCATGGCATACA
CCGCATCCGCAATTTCGAACTGGTCGCCGGCCCGCTGGTCCTTGTCGTCGCCGGCGCCGCCGCCCTCTGGGCCATCAGGA
TCTGCCACGGCACCGGCCCACTCTTCGCCATCCCGTCCACGCTGCACGGGTCGGCCCTGTTCCTCAAGGGCGGGGCCGCC
GTCACCAGCATCATCGGCATATGGGCATCGTTCGCGGTCAACATCCCCGACCTCAGCCGCTTCGTCCGCTCGCAGCGAGA
CCAGGCCATTGGCCAGCTCATCGGCCTGCCGGTCACCGCCGTCATCTTCACGCCCATGAGCATTCTCATCACCTCGGCCA
CCCTGGTGATGTTCGGAAAACCGATCTGGAACCCGGTCGAAATCCTGATGCGGATCCACAACACGCCGCTCACGCTCGCC
GGCGGCATCACGATCATCGTCGCGGCCCTGTCGGTCAACGTCGCCGGCAACATCGTCCCCGCCGCCTACGACCTGCTCAG
CCTGTTCCCCCGCCGGCTCGATTTCGACCGCGCGGGCATCACGGTCATCCTGCTCGGCCTGTTCGTCGCCCCCTGGCTGT
GGTTCGACCATCCGCAGACGGTCTTCGCCTTGCTCGGCCTGCTTTCGGCGGTGCTGGCACCCATCACCGGGATCATGCTG
GCCGACTACTATCTGGTCCGCCGCCAGACGATCGACCTCGCGCAGCTCTACTCATTCTCCGGCCTCTATGCGGGCCGGCG
GGGCTGGCGCCCGTCCGCCCTGGCGGCTACCGGCCTGACCCTGCTGCTGCTGCTGCCCCCCGCCCTGCTGCCGGCCGGCA
CCCTCCCGGGGCAGGATGCCATCAACGCGGTATCCTGGTTCCTGGGCATTGCGACGGGCGCACTGCTATACACCGGCTTC
AACCGCCACCCTCTTCACAGACCGAATGACCTGTGA

Upstream 100 bases:

>100_bases
CTAGAGGCCTATCCATTCCCACCGGTTCGCGGACATGCCCCCCCCCGGATCGGTGCCCTGGCGAGCATCCCTGTCCGACC
GTATGGAGCCATCCGGCCGG

Downstream 100 bases:

>100_bases
AGAGGGGCATGATCACATCAGACTACGATGATCATCATTTCTTCTGCTGCGTCGGTTTCGCCGGGGCAGCCGGGGCAGCC
GGGGCAGCCGGGGCAGCCGG

Product: allantoin permease

Products: NA

Alternate protein names: Allantoin transport protein [H]

Number of amino acids: Translated: 491; Mature: 490

Protein sequence:

>491_residues
MAPAQGPTGKDPMLDTTQTARDPLLWNPDIAPLPRQKRDWTWITLAAVWMGMVHNVVAYEAAAGLMAIGFSAMQCLLIVW
TAYCSLFVAMWFNARAGTEWGIPFCVLIRAAFGPRGAVIPVAIRAVVAIFWFSVQSYAASQALDAIAASLWPDWSSLHLP
DGTALGPWLAVTMIWGLHALVAAHGIHRIRNFELVAGPLVLVVAGAAALWAIRICHGTGPLFAIPSTLHGSALFLKGGAA
VTSIIGIWASFAVNIPDLSRFVRSQRDQAIGQLIGLPVTAVIFTPMSILITSATLVMFGKPIWNPVEILMRIHNTPLTLA
GGITIIVAALSVNVAGNIVPAAYDLLSLFPRRLDFDRAGITVILLGLFVAPWLWFDHPQTVFALLGLLSAVLAPITGIML
ADYYLVRRQTIDLAQLYSFSGLYAGRRGWRPSALAATGLTLLLLLPPALLPAGTLPGQDAINAVSWFLGIATGALLYTGF
NRHPLHRPNDL

Sequences:

>Translated_491_residues
MAPAQGPTGKDPMLDTTQTARDPLLWNPDIAPLPRQKRDWTWITLAAVWMGMVHNVVAYEAAAGLMAIGFSAMQCLLIVW
TAYCSLFVAMWFNARAGTEWGIPFCVLIRAAFGPRGAVIPVAIRAVVAIFWFSVQSYAASQALDAIAASLWPDWSSLHLP
DGTALGPWLAVTMIWGLHALVAAHGIHRIRNFELVAGPLVLVVAGAAALWAIRICHGTGPLFAIPSTLHGSALFLKGGAA
VTSIIGIWASFAVNIPDLSRFVRSQRDQAIGQLIGLPVTAVIFTPMSILITSATLVMFGKPIWNPVEILMRIHNTPLTLA
GGITIIVAALSVNVAGNIVPAAYDLLSLFPRRLDFDRAGITVILLGLFVAPWLWFDHPQTVFALLGLLSAVLAPITGIML
ADYYLVRRQTIDLAQLYSFSGLYAGRRGWRPSALAATGLTLLLLLPPALLPAGTLPGQDAINAVSWFLGIATGALLYTGF
NRHPLHRPNDL
>Mature_490_residues
APAQGPTGKDPMLDTTQTARDPLLWNPDIAPLPRQKRDWTWITLAAVWMGMVHNVVAYEAAAGLMAIGFSAMQCLLIVWT
AYCSLFVAMWFNARAGTEWGIPFCVLIRAAFGPRGAVIPVAIRAVVAIFWFSVQSYAASQALDAIAASLWPDWSSLHLPD
GTALGPWLAVTMIWGLHALVAAHGIHRIRNFELVAGPLVLVVAGAAALWAIRICHGTGPLFAIPSTLHGSALFLKGGAAV
TSIIGIWASFAVNIPDLSRFVRSQRDQAIGQLIGLPVTAVIFTPMSILITSATLVMFGKPIWNPVEILMRIHNTPLTLAG
GITIIVAALSVNVAGNIVPAAYDLLSLFPRRLDFDRAGITVILLGLFVAPWLWFDHPQTVFALLGLLSAVLAPITGIMLA
DYYLVRRQTIDLAQLYSFSGLYAGRRGWRPSALAATGLTLLLLLPPALLPAGTLPGQDAINAVSWFLGIATGALLYTGFN
RHPLHRPNDL

Specific function: Transport of allantoin [H]

COG id: COG1953

COG function: function code FH; Cytosine/uracil/thiamine/allantoin permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine-cytosine permease family [H]

Homologues:

Organism=Escherichia coli, GI87081749, Length=456, Percent_Identity=28.9473684210526, Blast_Score=196, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6319495, Length=435, Percent_Identity=26.4367816091954, Blast_Score=161, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6322219, Length=421, Percent_Identity=23.7529691211401, Blast_Score=156, Evalue=9e-39,
Organism=Saccharomyces cerevisiae, GI6319429, Length=421, Percent_Identity=23.2779097387173, Blast_Score=127, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6324766, Length=436, Percent_Identity=22.7064220183486, Blast_Score=95, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6323266, Length=443, Percent_Identity=20.9932279909707, Blast_Score=90, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6324645, Length=433, Percent_Identity=21.9399538106236, Blast_Score=88, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001248
- InterPro:   IPR012681 [H]

Pfam domain/function: PF02133 Transp_cyt_pur [H]

EC number: NA

Molecular weight: Translated: 52786; Mature: 52655

Theoretical pI: Translated: 9.38; Mature: 9.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPAQGPTGKDPMLDTTQTARDPLLWNPDIAPLPRQKRDWTWITLAAVWMGMVHNVVAYE
CCCCCCCCCCCCCCCCCHHHCCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
AAAGLMAIGFSAMQCLLIVWTAYCSLFVAMWFNARAGTEWGIPFCVLIRAAFGPRGAVIP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCHHH
VAIRAVVAIFWFSVQSYAASQALDAIAASLWPDWSSLHLPDGTALGPWLAVTMIWGLHAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCHHHHHHHHHHHHHHHH
VAAHGIHRIRNFELVAGPLVLVVAGAAALWAIRICHGTGPLFAIPSTLHGSALFLKGGAA
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCEEEEECCHH
VTSIIGIWASFAVNIPDLSRFVRSQRDQAIGQLIGLPVTAVIFTPMSILITSATLVMFGK
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC
PIWNPVEILMRIHNTPLTLAGGITIIVAALSVNVAGNIVPAAYDLLSLFPRRLDFDRAGI
CCCCHHHHHHHHCCCCEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCH
TVILLGLFVAPWLWFDHPQTVFALLGLLSAVLAPITGIMLADYYLVRRQTIDLAQLYSFS
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLYAGRRGWRPSALAATGLTLLLLLPPALLPAGTLPGQDAINAVSWFLGIATGALLYTGF
HHHCCCCCCCCHHHHHHHHHHHHHHCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
NRHPLHRPNDL
CCCCCCCCCCC
>Mature Secondary Structure 
APAQGPTGKDPMLDTTQTARDPLLWNPDIAPLPRQKRDWTWITLAAVWMGMVHNVVAYE
CCCCCCCCCCCCCCCCHHHCCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
AAAGLMAIGFSAMQCLLIVWTAYCSLFVAMWFNARAGTEWGIPFCVLIRAAFGPRGAVIP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCHHH
VAIRAVVAIFWFSVQSYAASQALDAIAASLWPDWSSLHLPDGTALGPWLAVTMIWGLHAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCHHHHHHHHHHHHHHHH
VAAHGIHRIRNFELVAGPLVLVVAGAAALWAIRICHGTGPLFAIPSTLHGSALFLKGGAA
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCEEEEECCHH
VTSIIGIWASFAVNIPDLSRFVRSQRDQAIGQLIGLPVTAVIFTPMSILITSATLVMFGK
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC
PIWNPVEILMRIHNTPLTLAGGITIIVAALSVNVAGNIVPAAYDLLSLFPRRLDFDRAGI
CCCCHHHHHHHHCCCCEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCH
TVILLGLFVAPWLWFDHPQTVFALLGLLSAVLAPITGIMLADYYLVRRQTIDLAQLYSFS
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLYAGRRGWRPSALAATGLTLLLLLPPALLPAGTLPGQDAINAVSWFLGIATGALLYTGF
HHHCCCCCCCCHHHHHHHHHHHHHHCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
NRHPLHRPNDL
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 11344136 [H]