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The map label for this gene is istB [H]

Identifier: 162146794

GI number: 162146794

Start: 993571

End: 994374

Strand: Direct

Name: istB [H]

Synonym: GDI_0976

Alternate gene names: 162146794

Gene position: 993571-994374 (Clockwise)

Preceding gene: 162146793

Following gene: 162146795

Centisome position: 25.19

GC content: 65.67

Gene sequence:

>804_bases
GTGATGACGTCCGTGAACCATGAAGCGCTGGTCGCGATGCTCGACCGGCTCAAGCTGACGGCGATCCGCGACCAGCTCGA
CACGCTGCTGGACGAGGCGGCGCGCTCGGACATGACGTTGCGCGAGGCGCTGGCGTTTCTGGTCGAACGCGAGATCGCCC
GCCGCGATGAACGACGCATCGCCATGGCGAGCAAGATGGCGCAGTTTCCGTTCGTGCGCGAGGTCGACGGGTTCGAGTTC
GACGCTCAGCCCTCCCTCGACCCGAAGCAGATCCGCGATCTGGCCGAAGGCCGCTGGATCGCGCACGGCGATACGGTCCT
GCTGCTGGGACCGCCGGGAACAGGGAAAACCCATCTGGCCGTGGCGCTCGGGCGCGAGGCGATCCGGCGGAATTACAGCG
TGCAGTTCGTCACCGCCGCGACGCTGGTGGCAATGCTGGCCAAGGCACATGCCGACGGCTCGCTGGACAAACAGCTGACG
ATCCTCTCGCGCCCGAAGCTGCTGGTCATCGACGAACTGGGCTACCTGCCGTTCGAGGCCAATGCCGCGCATTTGTTCTT
CCAGCTCGTGTCACGGCGCTACGAGCGCGGCTCAATCCTGATCACGTCCAACCGGTCGGTGGGCGAATGGGGCGAGGTCT
TCGGCGATCCGGTGGTCGCCACCGCCATCCTCGACCGCCTGCTGCACCATTCCACCGTAATCACCATCCGCGGCGACAGC
TACCGCCTGCGCGAAAAGCGACGCTCCGGCCTTCTGCAGAAGGCCGGAGCGTTCATCGAGAAAACCGAGGTCGCCACACC
ATGA

Upstream 100 bases:

>100_bases
GCGGACCGCGCGCCGCTCCGTTATCGCCTCCTGTGCCGATCCCCGAGCCGGCACTGCTCCGTCCGCTTGCCGAGTATGAG
GCCGTGGCGGGAGGTGCCTG

Downstream 100 bases:

>100_bases
AGGGGGCCAGTTCCTCACTTCGCCAAAGGGGTCAGTTCCTCGTTTCGTTTGACATCTCGAAGCGACACCTACTGCTTGGT
AACGGCTTCAGCATTGCCTG

Product: insertion sequence

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MMTSVNHEALVAMLDRLKLTAIRDQLDTLLDEAARSDMTLREALAFLVEREIARRDERRIAMASKMAQFPFVREVDGFEF
DAQPSLDPKQIRDLAEGRWIAHGDTVLLLGPPGTGKTHLAVALGREAIRRNYSVQFVTAATLVAMLAKAHADGSLDKQLT
ILSRPKLLVIDELGYLPFEANAAHLFFQLVSRRYERGSILITSNRSVGEWGEVFGDPVVATAILDRLLHHSTVITIRGDS
YRLREKRRSGLLQKAGAFIEKTEVATP

Sequences:

>Translated_267_residues
MMTSVNHEALVAMLDRLKLTAIRDQLDTLLDEAARSDMTLREALAFLVEREIARRDERRIAMASKMAQFPFVREVDGFEF
DAQPSLDPKQIRDLAEGRWIAHGDTVLLLGPPGTGKTHLAVALGREAIRRNYSVQFVTAATLVAMLAKAHADGSLDKQLT
ILSRPKLLVIDELGYLPFEANAAHLFFQLVSRRYERGSILITSNRSVGEWGEVFGDPVVATAILDRLLHHSTVITIRGDS
YRLREKRRSGLLQKAGAFIEKTEVATP
>Mature_267_residues
MMTSVNHEALVAMLDRLKLTAIRDQLDTLLDEAARSDMTLREALAFLVEREIARRDERRIAMASKMAQFPFVREVDGFEF
DAQPSLDPKQIRDLAEGRWIAHGDTVLLLGPPGTGKTHLAVALGREAIRRNYSVQFVTAATLVAMLAKAHADGSLDKQLT
ILSRPKLLVIDELGYLPFEANAAHLFFQLVSRRYERGSILITSNRSVGEWGEVFGDPVVATAILDRLLHHSTVITIRGDS
YRLREKRRSGLLQKAGAFIEKTEVATP

Specific function: One of two proteins expressed only when there is a tandem repeat of the IS21 insertion sequence, it is necessary for the transposition of plasmids with that tandem repeat [H]

COG id: COG1484

COG function: function code L; DNA replication protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IS21/IS1162 putative ATP-binding protein family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001270
- InterPro:   IPR002611 [H]

Pfam domain/function: PF01695 IstB [H]

EC number: NA

Molecular weight: Translated: 29802; Mature: 29802

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTSVNHEALVAMLDRLKLTAIRDQLDTLLDEAARSDMTLREALAFLVEREIARRDERRI
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
AMASKMAQFPFVREVDGFEFDAQPSLDPKQIRDLAEGRWIAHGDTVLLLGPPGTGKTHLA
HHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEECCCEEEEECCCCCCCHHHH
VALGREAIRRNYSVQFVTAATLVAMLAKAHADGSLDKQLTILSRPKLLVIDELGYLPFEA
HHHHHHHHHCCCCEEEHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEEECCCCCCCCC
NAAHLFFQLVSRRYERGSILITSNRSVGEWGEVFGDPVVATAILDRLLHHSTVITIRGDS
CHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHCCHHHHHHHHHHHHHCCEEEEEECCC
YRLREKRRSGLLQKAGAFIEKTEVATP
HHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MMTSVNHEALVAMLDRLKLTAIRDQLDTLLDEAARSDMTLREALAFLVEREIARRDERRI
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
AMASKMAQFPFVREVDGFEFDAQPSLDPKQIRDLAEGRWIAHGDTVLLLGPPGTGKTHLA
HHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEECCCEEEEECCCCCCCHHHH
VALGREAIRRNYSVQFVTAATLVAMLAKAHADGSLDKQLTILSRPKLLVIDELGYLPFEA
HHHHHHHHHCCCCEEEHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEEECCCCCCCCC
NAAHLFFQLVSRRYERGSILITSNRSVGEWGEVFGDPVVATAILDRLLHHSTVITIRGDS
CHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHCCHHHHHHHHHHHHHCCEEEEEECCC
YRLREKRRSGLLQKAGAFIEKTEVATP
HHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2540414 [H]