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The map label for this gene is mtlK [H]

Identifier: 162146708

GI number: 162146708

Start: 902869

End: 904332

Strand: Direct

Name: mtlK [H]

Synonym: GDI_0886

Alternate gene names: 162146708

Gene position: 902869-904332 (Clockwise)

Preceding gene: 162146707

Following gene: 162146710

Centisome position: 22.89

GC content: 69.33

Gene sequence:

>1464_bases
ATGATGAACCGGACAATTCTGGAGACTCTGCCGGCCGGCGTTTCCGCACCGGGTTATGACCCGTCCGCGATCGGCACGGG
GATCGTGCATTTCGGCGTCGGCAATTTCTTCCGCGCGCATGAGGCATTCTATGTCGATCGCTGCCTGGCCCTGCCGGGGC
AGCAGGATTGGGGCATTGCCGGCGTGGGCCTGACGGGGGGCGCCCGGTCGCAGGAGAAGGCCGAGACCTTCACCGCGCAG
GACTGCCTCTATTCCCTGACCGAGGCGGCGCCGGACGGCACGCGCACGGTGCGGGTGATCGGCGCGCTGCGGTCCTACCT
GCTGGCGCCCGCCGACCCGCAGGCGGTGCTGGACCTGCTGGCCGACTCCGCCACCCGCATCGTGACCATGACGATCACCG
AGGGCGGGTACAATATCGACGAGACGACCGGCGCCTTCCGCCTGGACACGCCGGCCGTGCGGTCGGACCTGTCGGGCGAG
GCCCCGCCCACCACCATCTTCGGCTTCGTCGTCGAGGCCCTCGCCCGCCGCAGGGCAGCCGGCGCAGGCCCGTTCACCGT
GATGTCGTGCGACAACCTGCGCCATAACGGTGACGTCGCCCGCACCGCCTTCCTGGGCTATGCGCAGGCCCGCGACCCCG
ACCTGGCGGCCTGGATCGCGGAAAACGTTACCTTCCCCAACGCCATGGTCGATCGCATCACGCCCACCGTCGATGCCGCC
ATCTCGGCCAGGCTGAACGCGGCCAGCGGCCTGGACGACGCGCTGCCGCTGGTGGCCGAGGATTTCACCCAGTGGGTGGT
CGAGGATAAATTCTGCGCCGGCCGGCCCGCGCTGGAGAAGGCGGGCGTGCAGTTCGTCGACGACGTGACGGGATACGAGC
AGGTGAAAGTGCGGATGCTGAACGCGTCGCACGCCCTGCTGGCGTTCTCCGGCCTGCTGCTGGGTTATCGGCTGATCGAC
GAAACCCTGCGCGACCCGGACCTGGAGCGTTTCGTGAACGCGTATCTGGAGCAGGACGTGATTCCGCGCCTCAGCACCCC
GCCGGGCCTGGATGCGCAGGAATACCGGCGCAGCGTGCTCAGCCGTTTCTCCAACCCGGCGATGGCCGACCAGTTGCTGC
GCATCGGCGGCGACGGGTCGTCCAAGATCCAGGTCTTCTGGACCGAGACGGTGCGGCGCGTGCTGGCCGATGGCGGGGAC
CTGTCCAGGATCGCCTTCGGCATTGCCGCCTATCTGGAAATGCTGCGCGGGGTGGACGAAAACGGGGCATCCTACACGCC
CTTCGAACCGACCCTGGACGCGGCGCAGATCGCGCTGGCCAGCGCGGACGACCTGACGGCGGCGCTGGCCCTGCCGTCCT
TCGACGGCTGGCGCGACGTCCAGACCCCGGCGCTGACCGATGCCATCGTGGCCGCGCGGCGGGCGATTCGCGACCAGGGC
GTACGGGCAGCGCTGTCCCGCTAG

Upstream 100 bases:

>100_bases
TGCTGCATGCCGACCTTCAGCGCGCCGTCCGCGCGCTGTGGTCCGGCAATGTCGCACCCCACCCTGTTGAGATAACGACC
GAGAAGGAGAATGAGGCGAC

Downstream 100 bases:

>100_bases
GAAGGTCTCTTGATCGGGGTGCAGGGCATCATGCCCTGTCGGGTCCGGGCAGGGCCCGGTCTGTGTCACACTCCACGCGC
CATCACTGCGCGCGAGGATC

Product: mannitol 2-dehydrogenase

Products: D-Fructuronate; H+; Nicotinamide adenine dinucleotide - reduced [C]

Alternate protein names: NA

Number of amino acids: Translated: 487; Mature: 487

Protein sequence:

>487_residues
MMNRTILETLPAGVSAPGYDPSAIGTGIVHFGVGNFFRAHEAFYVDRCLALPGQQDWGIAGVGLTGGARSQEKAETFTAQ
DCLYSLTEAAPDGTRTVRVIGALRSYLLAPADPQAVLDLLADSATRIVTMTITEGGYNIDETTGAFRLDTPAVRSDLSGE
APPTTIFGFVVEALARRRAAGAGPFTVMSCDNLRHNGDVARTAFLGYAQARDPDLAAWIAENVTFPNAMVDRITPTVDAA
ISARLNAASGLDDALPLVAEDFTQWVVEDKFCAGRPALEKAGVQFVDDVTGYEQVKVRMLNASHALLAFSGLLLGYRLID
ETLRDPDLERFVNAYLEQDVIPRLSTPPGLDAQEYRRSVLSRFSNPAMADQLLRIGGDGSSKIQVFWTETVRRVLADGGD
LSRIAFGIAAYLEMLRGVDENGASYTPFEPTLDAAQIALASADDLTAALALPSFDGWRDVQTPALTDAIVAARRAIRDQG
VRAALSR

Sequences:

>Translated_487_residues
MMNRTILETLPAGVSAPGYDPSAIGTGIVHFGVGNFFRAHEAFYVDRCLALPGQQDWGIAGVGLTGGARSQEKAETFTAQ
DCLYSLTEAAPDGTRTVRVIGALRSYLLAPADPQAVLDLLADSATRIVTMTITEGGYNIDETTGAFRLDTPAVRSDLSGE
APPTTIFGFVVEALARRRAAGAGPFTVMSCDNLRHNGDVARTAFLGYAQARDPDLAAWIAENVTFPNAMVDRITPTVDAA
ISARLNAASGLDDALPLVAEDFTQWVVEDKFCAGRPALEKAGVQFVDDVTGYEQVKVRMLNASHALLAFSGLLLGYRLID
ETLRDPDLERFVNAYLEQDVIPRLSTPPGLDAQEYRRSVLSRFSNPAMADQLLRIGGDGSSKIQVFWTETVRRVLADGGD
LSRIAFGIAAYLEMLRGVDENGASYTPFEPTLDAAQIALASADDLTAALALPSFDGWRDVQTPALTDAIVAARRAIRDQG
VRAALSR
>Mature_487_residues
MMNRTILETLPAGVSAPGYDPSAIGTGIVHFGVGNFFRAHEAFYVDRCLALPGQQDWGIAGVGLTGGARSQEKAETFTAQ
DCLYSLTEAAPDGTRTVRVIGALRSYLLAPADPQAVLDLLADSATRIVTMTITEGGYNIDETTGAFRLDTPAVRSDLSGE
APPTTIFGFVVEALARRRAAGAGPFTVMSCDNLRHNGDVARTAFLGYAQARDPDLAAWIAENVTFPNAMVDRITPTVDAA
ISARLNAASGLDDALPLVAEDFTQWVVEDKFCAGRPALEKAGVQFVDDVTGYEQVKVRMLNASHALLAFSGLLLGYRLID
ETLRDPDLERFVNAYLEQDVIPRLSTPPGLDAQEYRRSVLSRFSNPAMADQLLRIGGDGSSKIQVFWTETVRRVLADGGD
LSRIAFGIAAYLEMLRGVDENGASYTPFEPTLDAAQIALASADDLTAALALPSFDGWRDVQTPALTDAIVAARRAIRDQG
VRAALSR

Specific function: Hexuronate conversion to KDG. [C]

COG id: COG0246

COG function: function code G; Mannitol-1-phosphate/altronate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mannitol dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI1790779, Length=489, Percent_Identity=37.6278118609407, Blast_Score=317, Evalue=2e-87,
Organism=Escherichia coli, GI1787823, Length=434, Percent_Identity=39.8617511520737, Blast_Score=302, Evalue=3e-83,
Organism=Escherichia coli, GI1788497, Length=488, Percent_Identity=37.0901639344262, Blast_Score=299, Evalue=3e-82,
Organism=Escherichia coli, GI48994885, Length=491, Percent_Identity=26.2729124236253, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1790028, Length=211, Percent_Identity=27.0142180094787, Blast_Score=88, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6324401, Length=441, Percent_Identity=38.3219954648526, Blast_Score=324, Evalue=2e-89,
Organism=Saccharomyces cerevisiae, GI6320765, Length=441, Percent_Identity=38.3219954648526, Blast_Score=324, Evalue=2e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008927
- InterPro:   IPR013328
- InterPro:   IPR000669
- InterPro:   IPR013118
- InterPro:   IPR013131
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01232 Mannitol_dh; PF08125 Mannitol_dh_C [H]

EC number: 1.1.1.57 [C]

Molecular weight: Translated: 52236; Mature: 52236

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: PS00974 MANNITOL_DHGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMNRTILETLPAGVSAPGYDPSAIGTGIVHFGVGNFFRAHEAFYVDRCLALPGQQDWGIA
CCCCHHHHHCCCCCCCCCCCCHHHHCCHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCEE
GVGLTGGARSQEKAETFTAQDCLYSLTEAAPDGTRTVRVIGALRSYLLAPADPQAVLDLL
EECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHH
ADSATRIVTMTITEGGYNIDETTGAFRLDTPAVRSDLSGEAPPTTIFGFVVEALARRRAA
HCCCCEEEEEEEECCCCCCCCCCCCEEECCHHHHHCCCCCCCCHHHHHHHHHHHHHHHCC
GAGPFTVMSCDNLRHNGDVARTAFLGYAQARDPDLAAWIAENVTFPNAMVDRITPTVDAA
CCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHCCHHHHHH
ISARLNAASGLDDALPLVAEDFTQWVVEDKFCAGRPALEKAGVQFVDDVTGYEQVKVRML
HHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCHHHHHHCCHHHHEEEEE
NASHALLAFSGLLLGYRLIDETLRDPDLERFVNAYLEQDVIPRLSTPPGLDAQEYRRSVL
CCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
SRFSNPAMADQLLRIGGDGSSKIQVFWTETVRRVLADGGDLSRIAFGIAAYLEMLRGVDE
HHCCCHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCC
NGASYTPFEPTLDAAQIALASADDLTAALALPSFDGWRDVQTPALTDAIVAARRAIRDQG
CCCCCCCCCCCHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
VRAALSR
HHHHHCC
>Mature Secondary Structure
MMNRTILETLPAGVSAPGYDPSAIGTGIVHFGVGNFFRAHEAFYVDRCLALPGQQDWGIA
CCCCHHHHHCCCCCCCCCCCCHHHHCCHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCEE
GVGLTGGARSQEKAETFTAQDCLYSLTEAAPDGTRTVRVIGALRSYLLAPADPQAVLDLL
EECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHH
ADSATRIVTMTITEGGYNIDETTGAFRLDTPAVRSDLSGEAPPTTIFGFVVEALARRRAA
HCCCCEEEEEEEECCCCCCCCCCCCEEECCHHHHHCCCCCCCCHHHHHHHHHHHHHHHCC
GAGPFTVMSCDNLRHNGDVARTAFLGYAQARDPDLAAWIAENVTFPNAMVDRITPTVDAA
CCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHCCHHHHHH
ISARLNAASGLDDALPLVAEDFTQWVVEDKFCAGRPALEKAGVQFVDDVTGYEQVKVRML
HHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCHHHHHHCCHHHHEEEEE
NASHALLAFSGLLLGYRLIDETLRDPDLERFVNAYLEQDVIPRLSTPPGLDAQEYRRSVL
CCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
SRFSNPAMADQLLRIGGDGSSKIQVFWTETVRRVLADGGDLSRIAFGIAAYLEMLRGVDE
HHCCCHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCC
NGASYTPFEPTLDAAQIALASADDLTAALALPSFDGWRDVQTPALTDAIVAARRAIRDQG
CCCCCCCCCCCHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
VRAALSR
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+; NADH; NADPH [C]

Metal ions: No metal ion requirement [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 1 {D-fructuronic} 0.2 {NAD+}} 0.7 {NADPH}} 1.8 {mannonate}} 1.7 {mannonate}} 0.5 {fructuronate}} 0.03 {NADH}} [C]

Substrates: D-Mannonate; Nicotinamide adenine dinucleotide [C]

Specific reaction: D-Mannonate + Nicotinamide adenine dinucleotide <==> D-Fructuronate + H+ + Nicotinamide adenine dinucleotide - reduced [C]

General reaction: Redox reaction [C]

Inhibitor: Altronate; ATP; Fructuronate; Mannonate; NAD+; NADH; p-Chloromercuribenzoate; Tagaturonate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA