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The map label for this gene is fumA [H]

Identifier: 162146692

GI number: 162146692

Start: 887193

End: 888857

Strand: Direct

Name: fumA [H]

Synonym: GDI_0870

Alternate gene names: 162146692

Gene position: 887193-888857 (Clockwise)

Preceding gene: 162146691

Following gene: 162146693

Centisome position: 22.49

GC content: 66.07

Gene sequence:

>1665_bases
GTGAATGCGTCATCCACCCCCCCCAAACCACCCCTCAAGCCGTTCGTCTACGGCCCGCTCTTTCCGGGCCATGACGACGA
TACGCCCTGGAAGAAGCTGGAGATCGAGGGCGTGCACACCACCGTGTGCGACGGCCGCACCGTTCTGCATGTCAGGCCCG
AGGCACTGAGCGAACTGGCCGCCCGGGGCTTCCACGACATCGCCCACCTGCTGCGTCCGGGGCACCTGGCGCAGCTGGCC
AAGATCCTGAAGGACCCCGAGGCCTCGGACAATGACCGGTTCGTGGCGCTGGACCTGCTGAAGAACGCCTGCATCGCGGC
GGGCGGCGTGCTGCCGATGTGCCAGGATACCGGCACCGCCATCGTCTACGGCAAGAAGGGCCAGCGCGTCTGGGTCGACG
GGAACGAGGAGGAAGCGTTCTCGCGCGGGGTGTACGACACCTATACCCGCACCAGCCTGCGCTATTCGCAGATGGCGCCG
CTGTCGATGTTCGAGGAAGTGAACACCGGCACCAACCTGCCGGTGCAGTTCCATATCTCGGCATCGCCCGGCGACTACCA
TTCCGAACAGATGGATTTGATGTTCGTCGCCAAGGGCGGCGGGTCGGCCAACAAGACCTTCCTGTTTCAGGAAACCCGCG
CCCTGCTGTCCAGCAAGGAAGACCTGCTGAAATGGCTGGCCGTCAAGGTGCGCACGCTGGGCACCTCGGCCTGCCCGCCC
TATCACCTGGCCGTGGTGATCGGCGGCATGTCGGCCGAACAGACCCTGGAAACGGTGAAGCTGGCCTCGACGCACTGGCT
GGACACCCTGCCCACGCAGGGAAGCCCGCTGGGCCATGCCTTCCGCGACCTGGAGATGGAGCAGGAGGTCCTGAAGCTGA
CCCAGTCCCTGGGAATCGGCGCGCAGTTCGGCGGCAAGTATTTCTGCCATGACGTGCGCGTGGTGCGCCTGCCGCGCCAC
GGGGCGTCGCTGCCGGTGGGGATCGGCGTGTCCTGCTCGGCCGACCGGCAGATCAAGGCGCGCGTCACCGCCGACGGGTT
CTTCCTGGAACAGCTCGAAACCGACCCCGCGCGCTTCCTGCCCGAGACGACGGACGAGCATCTGGGCGGCGAGGTGGTGC
AGGTGGACCTGAACCAGCCGATGACCGAAATCCGCGCGCAATTGTCGCGTTATCCGGTCCGCACGCGCCTGTCGCTGACC
GGCACCATGGTCGTGGCGCGCGACATCGCCCACGCCAAGTTCCGCGAGCGGCTGGAACGCGGCGAGGGGCTGCCGCAATA
CCTGAAGGACCATCCCGTCTATTATGCCGGCCCGGCCAAGACGCCCGACGGGCTGCCGACCGGCTCGTTCGGTCCCACCA
CGGCCGGTCGCATGGACAGCTATGTCGCCATGCTGCAGGAAGCCGGCGGGTCGTTCGTCATGCTGGCCAAGGGCAACCGG
TCCAAGGCGGTGCGCGACGCCTGCCATGAATATGGCGGCTTCTATCTCGGCTCGGTCGGCGGGCCCGCGGCCCGGCTGGC
CAAGGACTGCATCCGCAAGGTCGAGGTCCTGGAATATCCGGAACTGGGCATGGAAGCGGTGTGGAAGATCGAGGTGGAGA
ATTTCCCCGCCTTCATCGTCATCGACGATAAGGGTAATGATTTCTACGAAGGCCTGACGGGCTGA

Upstream 100 bases:

>100_bases
ACCGCCCACCATCCGGGGGCCCGGATGCGGCCGGGCACCCCGTGAACCGCCCCCACCCGGAAGCAATCCGGGGGAGACAG
GCACAGGGAGAACGTCCGAA

Downstream 100 bases:

>100_bases
GGCGGGGCGATTCGCCCCGTCACCCCTGGGTCAAGGGAGACCTCCGTTCCATGCGCTTTACCGTCGATCGGCTGGACCAT
GTCGTCATCACCGTCCGTGA

Product: fumarate hydratase class

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 554; Mature: 554

Protein sequence:

>554_residues
MNASSTPPKPPLKPFVYGPLFPGHDDDTPWKKLEIEGVHTTVCDGRTVLHVRPEALSELAARGFHDIAHLLRPGHLAQLA
KILKDPEASDNDRFVALDLLKNACIAAGGVLPMCQDTGTAIVYGKKGQRVWVDGNEEEAFSRGVYDTYTRTSLRYSQMAP
LSMFEEVNTGTNLPVQFHISASPGDYHSEQMDLMFVAKGGGSANKTFLFQETRALLSSKEDLLKWLAVKVRTLGTSACPP
YHLAVVIGGMSAEQTLETVKLASTHWLDTLPTQGSPLGHAFRDLEMEQEVLKLTQSLGIGAQFGGKYFCHDVRVVRLPRH
GASLPVGIGVSCSADRQIKARVTADGFFLEQLETDPARFLPETTDEHLGGEVVQVDLNQPMTEIRAQLSRYPVRTRLSLT
GTMVVARDIAHAKFRERLERGEGLPQYLKDHPVYYAGPAKTPDGLPTGSFGPTTAGRMDSYVAMLQEAGGSFVMLAKGNR
SKAVRDACHEYGGFYLGSVGGPAARLAKDCIRKVEVLEYPELGMEAVWKIEVENFPAFIVIDDKGNDFYEGLTG

Sequences:

>Translated_554_residues
MNASSTPPKPPLKPFVYGPLFPGHDDDTPWKKLEIEGVHTTVCDGRTVLHVRPEALSELAARGFHDIAHLLRPGHLAQLA
KILKDPEASDNDRFVALDLLKNACIAAGGVLPMCQDTGTAIVYGKKGQRVWVDGNEEEAFSRGVYDTYTRTSLRYSQMAP
LSMFEEVNTGTNLPVQFHISASPGDYHSEQMDLMFVAKGGGSANKTFLFQETRALLSSKEDLLKWLAVKVRTLGTSACPP
YHLAVVIGGMSAEQTLETVKLASTHWLDTLPTQGSPLGHAFRDLEMEQEVLKLTQSLGIGAQFGGKYFCHDVRVVRLPRH
GASLPVGIGVSCSADRQIKARVTADGFFLEQLETDPARFLPETTDEHLGGEVVQVDLNQPMTEIRAQLSRYPVRTRLSLT
GTMVVARDIAHAKFRERLERGEGLPQYLKDHPVYYAGPAKTPDGLPTGSFGPTTAGRMDSYVAMLQEAGGSFVMLAKGNR
SKAVRDACHEYGGFYLGSVGGPAARLAKDCIRKVEVLEYPELGMEAVWKIEVENFPAFIVIDDKGNDFYEGLTG
>Mature_554_residues
MNASSTPPKPPLKPFVYGPLFPGHDDDTPWKKLEIEGVHTTVCDGRTVLHVRPEALSELAARGFHDIAHLLRPGHLAQLA
KILKDPEASDNDRFVALDLLKNACIAAGGVLPMCQDTGTAIVYGKKGQRVWVDGNEEEAFSRGVYDTYTRTSLRYSQMAP
LSMFEEVNTGTNLPVQFHISASPGDYHSEQMDLMFVAKGGGSANKTFLFQETRALLSSKEDLLKWLAVKVRTLGTSACPP
YHLAVVIGGMSAEQTLETVKLASTHWLDTLPTQGSPLGHAFRDLEMEQEVLKLTQSLGIGAQFGGKYFCHDVRVVRLPRH
GASLPVGIGVSCSADRQIKARVTADGFFLEQLETDPARFLPETTDEHLGGEVVQVDLNQPMTEIRAQLSRYPVRTRLSLT
GTMVVARDIAHAKFRERLERGEGLPQYLKDHPVYYAGPAKTPDGLPTGSFGPTTAGRMDSYVAMLQEAGGSFVMLAKGNR
SKAVRDACHEYGGFYLGSVGGPAARLAKDCIRKVEVLEYPELGMEAVWKIEVENFPAFIVIDDKGNDFYEGLTG

Specific function: It functions in the generation of fumarate for use as an anaerobic electron acceptor [H]

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1787897, Length=540, Percent_Identity=61.6666666666667, Blast_Score=702, Evalue=0.0,
Organism=Escherichia coli, GI1790564, Length=541, Percent_Identity=61.3678373382625, Blast_Score=702, Evalue=0.0,
Organism=Escherichia coli, GI1789443, Length=172, Percent_Identity=30.8139534883721, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1789442, Length=304, Percent_Identity=25.6578947368421, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 60651; Mature: 60651

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNASSTPPKPPLKPFVYGPLFPGHDDDTPWKKLEIEGVHTTVCDGRTVLHVRPEALSELA
CCCCCCCCCCCCCCEEECCCCCCCCCCCCCEEEEECCCEEEEECCCEEEEECHHHHHHHH
ARGFHDIAHLLRPGHLAQLAKILKDPEASDNDRFVALDLLKNACIAAGGVLPMCQDTGTA
HCCHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHCCCCCEEECCCCCE
IVYGKKGQRVWVDGNEEEAFSRGVYDTYTRTSLRYSQMAPLSMFEEVNTGTNLPVQFHIS
EEECCCCCEEEEECCHHHHHHCCCCHHHHHHHCCHHHCCHHHHHHHHCCCCCCCEEEEEE
ASPGDYHSEQMDLMFVAKGGGSANKTFLFQETRALLSSKEDLLKWLAVKVRTLGTSACPP
CCCCCCCCCCEEEEEEECCCCCCCCEEEHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCC
YHLAVVIGGMSAEQTLETVKLASTHWLDTLPTQGSPLGHAFRDLEMEQEVLKLTQSLGIG
EEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
AQFGGKYFCHDVRVVRLPRHGASLPVGIGVSCSADRQIKARVTADGFFLEQLETDPARFL
CCCCCEEEEECEEEEEECCCCCCCCEECCCCCCCCCCEEEEEEECCEEHHHHCCCHHHCC
PETTDEHLGGEVVQVDLNQPMTEIRAQLSRYPVRTRLSLTGTMVVARDIAHAKFRERLER
CCCCHHHCCCEEEEEECCCCHHHHHHHHHHCCCEEEEEEEEEHHHHHHHHHHHHHHHHHH
GEGLPQYLKDHPVYYAGPAKTPDGLPTGSFGPTTAGRMDSYVAMLQEAGGSFVMLAKGNR
CCCCHHHHHHCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCC
SKAVRDACHEYGGFYLGSVGGPAARLAKDCIRKVEVLEYPELGMEAVWKIEVENFPAFIV
CHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCEEEE
IDDKGNDFYEGLTG
EECCCCHHHCCCCC
>Mature Secondary Structure
MNASSTPPKPPLKPFVYGPLFPGHDDDTPWKKLEIEGVHTTVCDGRTVLHVRPEALSELA
CCCCCCCCCCCCCCEEECCCCCCCCCCCCCEEEEECCCEEEEECCCEEEEECHHHHHHHH
ARGFHDIAHLLRPGHLAQLAKILKDPEASDNDRFVALDLLKNACIAAGGVLPMCQDTGTA
HCCHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHCCCCCEEECCCCCE
IVYGKKGQRVWVDGNEEEAFSRGVYDTYTRTSLRYSQMAPLSMFEEVNTGTNLPVQFHIS
EEECCCCCEEEEECCHHHHHHCCCCHHHHHHHCCHHHCCHHHHHHHHCCCCCCCEEEEEE
ASPGDYHSEQMDLMFVAKGGGSANKTFLFQETRALLSSKEDLLKWLAVKVRTLGTSACPP
CCCCCCCCCCEEEEEEECCCCCCCCEEEHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCC
YHLAVVIGGMSAEQTLETVKLASTHWLDTLPTQGSPLGHAFRDLEMEQEVLKLTQSLGIG
EEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
AQFGGKYFCHDVRVVRLPRHGASLPVGIGVSCSADRQIKARVTADGFFLEQLETDPARFL
CCCCCEEEEECEEEEEECCCCCCCCEECCCCCCCCCCEEEEEEECCEEHHHHCCCHHHCC
PETTDEHLGGEVVQVDLNQPMTEIRAQLSRYPVRTRLSLTGTMVVARDIAHAKFRERLER
CCCCHHHCCCEEEEEECCCCHHHHHHHHHHCCCEEEEEEEEEHHHHHHHHHHHHHHHHHH
GEGLPQYLKDHPVYYAGPAKTPDGLPTGSFGPTTAGRMDSYVAMLQEAGGSFVMLAKGNR
CCCCHHHHHHCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCC
SKAVRDACHEYGGFYLGSVGGPAARLAKDCIRKVEVLEYPELGMEAVWKIEVENFPAFIV
CHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCEEEE
IDDKGNDFYEGLTG
EECCCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2656658; 7610040; 9278503 [H]