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The map label for this gene is tilS [H]

Identifier: 162146631

GI number: 162146631

Start: 826473

End: 827765

Strand: Direct

Name: tilS [H]

Synonym: GDI_0809

Alternate gene names: 162146631

Gene position: 826473-827765 (Clockwise)

Preceding gene: 162146630

Following gene: 162146632

Centisome position: 20.95

GC content: 77.8

Gene sequence:

>1293_bases
CTGACCGGGGACCCCGCCGGGGGCCCGACCCGCTCGGGCGTACTGGATGACGCCCGTTTCGCCGCCATCATGGCGGGGCT
TGGTCCCTGGCTGCCCGATGGGCCGGACATGCCCCCGGTGGGGGTGGCGGTATCGGGCGGCGCCGACAGTCTATGCCTGG
CCTACCTGGCCGCGCGCTGGCGCCGCGCGGTCCGTGCCCTGATCGTCGATCACGGCCTGCGTCCCGCCGCGGCGGCCGAG
GCCGCGCTGACCCGGCGGCGGCTGCATGCCCTGGGCATTCCCGCCGACATCCTGGTTCTCGACGGCCTGCGGCCCGGGCC
CGGCCTGGCCGACCGGGCCCGTCGCGCACGCTATGCGGCGCTGGCCCGGGCCTGCCGGCGGGCGGGCATCGTCGACCTGC
TGCTGGGCCATCATGCCGGCGACCAGGCGGAAACGATCGCCATCCGCCGCCGCGCCGCCAGCGGGCCGGACGGTCTGGCC
GGCATGGCCTCCGTCAGCTTCACCGCCGATCTGCGTCTGGTGCGTCCCCTGCTGGGTGTCGATCGCGACCGCCTGCGCGC
GACGCTGCGCCGCGCCGGCCTGGCATGGGTCGAAGACCCGTCGAATGCCGATCCGCGCGCCGAACGGGCCCGGGTGCGCG
CGGCGCTGGACGACGACCCGGCGGCACGCGGGCCGCTCCGCGCGGCGGCGTCGCGGGCCGGCGCGGCGCGCATGGACCGT
GATGCCAGGCAGGCCGCGCTGCTGGCCCGGAACGTGACGATGGCGGAAGGGGGCTGGGTGGCGCTGGCCGACGATCCGAC
CTGCCCCAGGGCGCTGGCGTTCCTGATGCGGGCGGTGTCGGGGTCGGACTACCTGCCCCCTTCGCGGGCGGTCGCGGCCC
TGTGCGCCGCCCCGCGGCCGGCGACGCTGGGGGGCACCTGCCTGATGGCGGCGGGGCCGGCCCTGAAGCGCGCCGGCCAT
CGATGGCTGCTGCTGCGCGAGGAAGCGGCGATGCAGCCCCCGGTCGTCGCCCGGCCGGACTGCGTGTGGGACGGGCGCTT
CGTCCTGTCGGCGCCGGCGGGCACGAACCTGGACGGTGTGACGCTGGGCGCGGCCGGCCCGTCCGGGGCCTGGGCGCCCC
CCGGCTGGCGAACGCAATGGCCCGCGCGCGCGTTGCGCACCCTGCCGGCGCTGCATCGCGCGGGGCGGGTGGTGGCGGTT
CCGACCATGGGGATCTGCCACGCGGCGGACCTGGCGGGGGTGGCCCTGATGCTGACCCCGGGGGCGGTAGCGGCCGAGAC
CGCCCGCTTTTGA

Upstream 100 bases:

>100_bases
GCGCTGGCGAAGCTGAAGGCGGAATTCCCCACCCCCGCCCCCCGCGTCCGCAGCGCGCAGGCCATCTACCGCACCCGTGC
CGGATGCCACTGATATCAGG

Downstream 100 bases:

>100_bases
CAAGAAAGAGGGGCCATGGGGGTGTGAATGTCCCCCGAACCGCCTATGTATGGGTGAACATTAGGTTGAAAAGGCGTTTG
GCCGCTGGTTGGCGCCAGCG

Product: tRNA(Ile)-lysidine synthase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MTGDPAGGPTRSGVLDDARFAAIMAGLGPWLPDGPDMPPVGVAVSGGADSLCLAYLAARWRRAVRALIVDHGLRPAAAAE
AALTRRRLHALGIPADILVLDGLRPGPGLADRARRARYAALARACRRAGIVDLLLGHHAGDQAETIAIRRRAASGPDGLA
GMASVSFTADLRLVRPLLGVDRDRLRATLRRAGLAWVEDPSNADPRAERARVRAALDDDPAARGPLRAAASRAGAARMDR
DARQAALLARNVTMAEGGWVALADDPTCPRALAFLMRAVSGSDYLPPSRAVAALCAAPRPATLGGTCLMAAGPALKRAGH
RWLLLREEAAMQPPVVARPDCVWDGRFVLSAPAGTNLDGVTLGAAGPSGAWAPPGWRTQWPARALRTLPALHRAGRVVAV
PTMGICHAADLAGVALMLTPGAVAAETARF

Sequences:

>Translated_430_residues
MTGDPAGGPTRSGVLDDARFAAIMAGLGPWLPDGPDMPPVGVAVSGGADSLCLAYLAARWRRAVRALIVDHGLRPAAAAE
AALTRRRLHALGIPADILVLDGLRPGPGLADRARRARYAALARACRRAGIVDLLLGHHAGDQAETIAIRRRAASGPDGLA
GMASVSFTADLRLVRPLLGVDRDRLRATLRRAGLAWVEDPSNADPRAERARVRAALDDDPAARGPLRAAASRAGAARMDR
DARQAALLARNVTMAEGGWVALADDPTCPRALAFLMRAVSGSDYLPPSRAVAALCAAPRPATLGGTCLMAAGPALKRAGH
RWLLLREEAAMQPPVVARPDCVWDGRFVLSAPAGTNLDGVTLGAAGPSGAWAPPGWRTQWPARALRTLPALHRAGRVVAV
PTMGICHAADLAGVALMLTPGAVAAETARF
>Mature_429_residues
TGDPAGGPTRSGVLDDARFAAIMAGLGPWLPDGPDMPPVGVAVSGGADSLCLAYLAARWRRAVRALIVDHGLRPAAAAEA
ALTRRRLHALGIPADILVLDGLRPGPGLADRARRARYAALARACRRAGIVDLLLGHHAGDQAETIAIRRRAASGPDGLAG
MASVSFTADLRLVRPLLGVDRDRLRATLRRAGLAWVEDPSNADPRAERARVRAALDDDPAARGPLRAAASRAGAARMDRD
ARQAALLARNVTMAEGGWVALADDPTCPRALAFLMRAVSGSDYLPPSRAVAALCAAPRPATLGGTCLMAAGPALKRAGHR
WLLLREEAAMQPPVVARPDCVWDGRFVLSAPAGTNLDGVTLGAAGPSGAWAPPGWRTQWPARALRTLPALHRAGRVVAVP
TMGICHAADLAGVALMLTPGAVAAETARF

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=202, Percent_Identity=34.6534653465347, Blast_Score=94, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 44685; Mature: 44554

Theoretical pI: Translated: 11.53; Mature: 11.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGDPAGGPTRSGVLDDARFAAIMAGLGPWLPDGPDMPPVGVAVSGGADSLCLAYLAARW
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHH
RRAVRALIVDHGLRPAAAAEAALTRRRLHALGIPADILVLDGLRPGPGLADRARRARYAA
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHEEEECCCCCCCCHHHHHHHHHHHH
LARACRRAGIVDLLLGHHAGDQAETIAIRRRAASGPDGLAGMASVSFTADLRLVRPLLGV
HHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
DRDRLRATLRRAGLAWVEDPSNADPRAERARVRAALDDDPAARGPLRAAASRAGAARMDR
CHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHCCHHHHHH
DARQAALLARNVTMAEGGWVALADDPTCPRALAFLMRAVSGSDYLPPSRAVAALCAAPRP
HHHHHHHHHHCCEEECCCEEEEECCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCC
ATLGGTCLMAAGPALKRAGHRWLLLREEAAMQPPVVARPDCVWDGRFVLSAPAGTNLDGV
CCCCCEEHHHCCHHHHHCCCEEEEEEHHHCCCCCCCCCCCCEECCCEEEECCCCCCCCCE
TLGAAGPSGAWAPPGWRTQWPARALRTLPALHRAGRVVAVPTMGICHAADLAGVALMLTP
EEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHCEEEEECC
GAVAAETARF
CHHHHHHCCC
>Mature Secondary Structure 
TGDPAGGPTRSGVLDDARFAAIMAGLGPWLPDGPDMPPVGVAVSGGADSLCLAYLAARW
CCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHH
RRAVRALIVDHGLRPAAAAEAALTRRRLHALGIPADILVLDGLRPGPGLADRARRARYAA
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHEEEECCCCCCCCHHHHHHHHHHHH
LARACRRAGIVDLLLGHHAGDQAETIAIRRRAASGPDGLAGMASVSFTADLRLVRPLLGV
HHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
DRDRLRATLRRAGLAWVEDPSNADPRAERARVRAALDDDPAARGPLRAAASRAGAARMDR
CHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHCCHHHHHH
DARQAALLARNVTMAEGGWVALADDPTCPRALAFLMRAVSGSDYLPPSRAVAALCAAPRP
HHHHHHHHHHCCEEECCCEEEEECCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCC
ATLGGTCLMAAGPALKRAGHRWLLLREEAAMQPPVVARPDCVWDGRFVLSAPAGTNLDGV
CCCCCEEHHHCCHHHHHCCCEEEEEEHHHCCCCCCCCCCCCEECCCEEEECCCCCCCCCE
TLGAAGPSGAWAPPGWRTQWPARALRTLPALHRAGRVVAVPTMGICHAADLAGVALMLTP
EEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHCEEEEECC
GAVAAETARF
CHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA