Click here to switch to the map view.

The map label for this gene is tolB [H]

Identifier: 162146628

GI number: 162146628

Start: 823356

End: 824744

Strand: Direct

Name: tolB [H]

Synonym: GDI_0806

Alternate gene names: 162146628

Gene position: 823356-824744 (Clockwise)

Preceding gene: 162146627

Following gene: 162146629

Centisome position: 20.88

GC content: 69.4

Gene sequence:

>1389_bases
ATGCGTCCCTGTGACCTTCCGCCGACTTCCCCCTGGATTTCCGATACCGATGCATCGCTGCTGGCGACCCATCTCGGCCG
CCGCATGCTGCTGGGAACGGGCGGCGGCATCGCGGCCGGCATCCTGGCCGCGCCGCTGTCGGCCCTTGCGCAGGGCGCGC
CCGCGGCGGGCCCGGGTGCCGCCGAAATCACGGTGGACCAGGCCCGCACGGCGCCGATTCCCATCGTCATTCCGGCGCTG
GGCGGCGACGCGGGCGCGCTGGTCAGCAGCGTGATCTCGGGCGACCTGGGGAATTGCGGCCTGTTCTCCCCGATCAGCGG
GTCGCTGCCCGTGGGGGTGCCGGATTTCGGAACATACAAGTCGCTGGGCGCCCGCGCGCTGGTCAGCGGTTCGGCGACGA
CCGGCGGTGGCGGCCTGCGCGTCGAATTCCGCCTGTGGGACGTGCTGACCGGCCAGCAGATCCAGGGCACGGCCTATACG
GCGGGCGCCGGCGACGCGCGCAAGATCGCGCACATCATCGCCGATGTGATCTATGAACGGCTGCTGGGCGAAAAGGGCTA
TTTCAACACCCGCATTGCCTATATCGCGCGGTCCGGTCCGCGCGAGCACCAGACGACGCGGCTGGCGATCATGGACCAGG
ACGGCGCCAACAGCCATTACCTGTCGGGGGGCCAATGGCTGACCCTGACCCCGCGCTTCAGCCCGACCAGCGACCAACTG
GCGTTCATGTCGTACGCCAACAACCGCCCGCGCGTGTACATGCTCAACCTGGCGACGGGCCGGCAGCAGTTGCTGGGCGA
TTTCGAAGGGATCTCGTTCGCGCCGCGTTTCGCGCCGGACGGGCGGGCGGTCATCCTGTCGGCCACGCGCGGCGCGGGGT
CGGACATCTATAGCGTGGACCTGGTCACGCGGGCCAAGCGGCAGATCACCAGTTCCGGCGCCATCGATACCAGCCCGTGC
TACAGCCCGGACGGGTCGCAGATCGTCTTCAACTCGGACCGGGGCGGTTCGCCGCAGCTTTATATCATGAGCGCGGGCGG
CGGCGACGCGCGGCGTATTTCGTACGGCAACGGCGTGTACGGCTCGCCGGTCTGGTCACCGCGCGGGGATTTGATCGCCT
TCACCCGGATCGCCAACGGCAGCTTCTCGCTGGGCGTGATGGCCCCCGACGGCACGGGCGAGCGGATCATGACCCAGGGC
TTCACCGTCGAAAGCCCCACCTTCTGCCCGAACGGACGGGTGATCGCCTTCTGCCGCCAGACGGCGGCGGGGGCCGGCGG
GGCAGGGTTTTCCTCGGGCATCAGCACCGTCGATATCACCGGCTTCCACGAACGCGCGATTCCGGCCGGCGGCGCGTCCG
ACCCGGCCTGGTCGCCGCTGAACGGTTAA

Upstream 100 bases:

>100_bases
GATGCTGGGGCAAACGCGGCAGCTCAAATTCGTTTTCCGCCCATGAAGCAGCACAACCTGCCGCAGCCCATGCACGGGCG
GTGCGTTCGAGGAGAAGACC

Downstream 100 bases:

>100_bases
GATTTAAAGACCGGGGAAGCGCGTTACTGTGCTTTTCCGGTCAATACTGAAACAAGATATCGGACATGTGTTCCGGGCAT
CTTGACGCTTCGTCGCGTCT

Product: translocation protein TolB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MRPCDLPPTSPWISDTDASLLATHLGRRMLLGTGGGIAAGILAAPLSALAQGAPAAGPGAAEITVDQARTAPIPIVIPAL
GGDAGALVSSVISGDLGNCGLFSPISGSLPVGVPDFGTYKSLGARALVSGSATTGGGGLRVEFRLWDVLTGQQIQGTAYT
AGAGDARKIAHIIADVIYERLLGEKGYFNTRIAYIARSGPREHQTTRLAIMDQDGANSHYLSGGQWLTLTPRFSPTSDQL
AFMSYANNRPRVYMLNLATGRQQLLGDFEGISFAPRFAPDGRAVILSATRGAGSDIYSVDLVTRAKRQITSSGAIDTSPC
YSPDGSQIVFNSDRGGSPQLYIMSAGGGDARRISYGNGVYGSPVWSPRGDLIAFTRIANGSFSLGVMAPDGTGERIMTQG
FTVESPTFCPNGRVIAFCRQTAAGAGGAGFSSGISTVDITGFHERAIPAGGASDPAWSPLNG

Sequences:

>Translated_462_residues
MRPCDLPPTSPWISDTDASLLATHLGRRMLLGTGGGIAAGILAAPLSALAQGAPAAGPGAAEITVDQARTAPIPIVIPAL
GGDAGALVSSVISGDLGNCGLFSPISGSLPVGVPDFGTYKSLGARALVSGSATTGGGGLRVEFRLWDVLTGQQIQGTAYT
AGAGDARKIAHIIADVIYERLLGEKGYFNTRIAYIARSGPREHQTTRLAIMDQDGANSHYLSGGQWLTLTPRFSPTSDQL
AFMSYANNRPRVYMLNLATGRQQLLGDFEGISFAPRFAPDGRAVILSATRGAGSDIYSVDLVTRAKRQITSSGAIDTSPC
YSPDGSQIVFNSDRGGSPQLYIMSAGGGDARRISYGNGVYGSPVWSPRGDLIAFTRIANGSFSLGVMAPDGTGERIMTQG
FTVESPTFCPNGRVIAFCRQTAAGAGGAGFSSGISTVDITGFHERAIPAGGASDPAWSPLNG
>Mature_462_residues
MRPCDLPPTSPWISDTDASLLATHLGRRMLLGTGGGIAAGILAAPLSALAQGAPAAGPGAAEITVDQARTAPIPIVIPAL
GGDAGALVSSVISGDLGNCGLFSPISGSLPVGVPDFGTYKSLGARALVSGSATTGGGGLRVEFRLWDVLTGQQIQGTAYT
AGAGDARKIAHIIADVIYERLLGEKGYFNTRIAYIARSGPREHQTTRLAIMDQDGANSHYLSGGQWLTLTPRFSPTSDQL
AFMSYANNRPRVYMLNLATGRQQLLGDFEGISFAPRFAPDGRAVILSATRGAGSDIYSVDLVTRAKRQITSSGAIDTSPC
YSPDGSQIVFNSDRGGSPQLYIMSAGGGDARRISYGNGVYGSPVWSPRGDLIAFTRIANGSFSLGVMAPDGTGERIMTQG
FTVESPTFCPNGRVIAFCRQTAAGAGGAGFSSGISTVDITGFHERAIPAGGASDPAWSPLNG

Specific function: Involved in the tonB-independent uptake of proteins [H]

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family [H]

Homologues:

Organism=Escherichia coli, GI1786961, Length=386, Percent_Identity=26.1658031088083, Blast_Score=134, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011659
- InterPro:   IPR006311
- InterPro:   IPR014167
- InterPro:   IPR007195
- InterPro:   IPR015943 [H]

Pfam domain/function: PF07676 PD40; PF04052 TolB_N [H]

EC number: NA

Molecular weight: Translated: 47679; Mature: 47679

Theoretical pI: Translated: 7.88; Mature: 7.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPCDLPPTSPWISDTDASLLATHLGRRMLLGTGGGIAAGILAAPLSALAQGAPAAGPGA
CCCCCCCCCCCCCCCCCHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCC
AEITVDQARTAPIPIVIPALGGDAGALVSSVISGDLGNCGLFSPISGSLPVGVPDFGTYK
EEEEECCCCCCCCCEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHH
SLGARALVSGSATTGGGGLRVEFRLWDVLTGQQIQGTAYTAGAGDARKIAHIIADVIYER
HHCCEEEEECCCCCCCCCEEEEEEEEEECCCCEECCEEEECCCCCHHHHHHHHHHHHHHH
LLGEKGYFNTRIAYIARSGPREHQTTRLAIMDQDGANSHYLSGGQWLTLTPRFSPTSDQL
HHCCCCCCCEEEEEEECCCCCCCCEEEEEEEECCCCCCCEECCCCEEEEECCCCCCCCCE
AFMSYANNRPRVYMLNLATGRQQLLGDFEGISFAPRFAPDGRAVILSATRGAGSDIYSVD
EEEECCCCCCEEEEEEECCCHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEH
LVTRAKRQITSSGAIDTSPCYSPDGSQIVFNSDRGGSPQLYIMSAGGGDARRISYGNGVY
HHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEECCCCCCEEEECCCCCC
GSPVWSPRGDLIAFTRIANGSFSLGVMAPDGTGERIMTQGFTVESPTFCPNGRVIAFCRQ
CCCCCCCCCCEEEEEEECCCCEEEEEECCCCCCCEEEECCEEECCCCCCCCCCEEEEEEC
TAAGAGGAGFSSGISTVDITGFHERAIPAGGASDPAWSPLNG
CCCCCCCCCHHCCCCEEEECCCHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRPCDLPPTSPWISDTDASLLATHLGRRMLLGTGGGIAAGILAAPLSALAQGAPAAGPGA
CCCCCCCCCCCCCCCCCHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCC
AEITVDQARTAPIPIVIPALGGDAGALVSSVISGDLGNCGLFSPISGSLPVGVPDFGTYK
EEEEECCCCCCCCCEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHH
SLGARALVSGSATTGGGGLRVEFRLWDVLTGQQIQGTAYTAGAGDARKIAHIIADVIYER
HHCCEEEEECCCCCCCCCEEEEEEEEEECCCCEECCEEEECCCCCHHHHHHHHHHHHHHH
LLGEKGYFNTRIAYIARSGPREHQTTRLAIMDQDGANSHYLSGGQWLTLTPRFSPTSDQL
HHCCCCCCCEEEEEEECCCCCCCCEEEEEEEECCCCCCCEECCCCEEEEECCCCCCCCCE
AFMSYANNRPRVYMLNLATGRQQLLGDFEGISFAPRFAPDGRAVILSATRGAGSDIYSVD
EEEECCCCCCEEEEEEECCCHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEH
LVTRAKRQITSSGAIDTSPCYSPDGSQIVFNSDRGGSPQLYIMSAGGGDARRISYGNGVY
HHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEECCCCCCEEEECCCCCC
GSPVWSPRGDLIAFTRIANGSFSLGVMAPDGTGERIMTQGFTVESPTFCPNGRVIAFCRQ
CCCCCCCCCCEEEEEEECCCCEEEEEECCCCCCCEEEECCEEECCCCCCCCCCEEEEEEC
TAAGAGGAGFSSGISTVDITGFHERAIPAGGASDPAWSPLNG
CCCCCCCCCHHCCCCEEEECCCHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA