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The map label for this gene is phrB [H]

Identifier: 162146574

GI number: 162146574

Start: 767613

End: 769004

Strand: Direct

Name: phrB [H]

Synonym: GDI_0751

Alternate gene names: 162146574

Gene position: 767613-769004 (Clockwise)

Preceding gene: 162146573

Following gene: 162146580

Centisome position: 19.46

GC content: 66.52

Gene sequence:

>1392_bases
ATGGTGAACCTGGCGCCTGCCCCGACCATCGTCTGGTTTCGCGAGGATTTCCGCATCGCGGACAACATGGCGTTGCTCGA
GGCGGAAAAACGGGGCCAGCCGACTCTCTGTATCGTCATTCTCGATGATGCCCATCTGCCCGGTGCCGCGGCGCGGTGGT
GGCTGCACGGCGCGATCCGCTCGCTGGACGAAACCCTGCGCGGGATGGGGGGCGCCCTTCATGTCTTTCGCGGCGCGGCC
CGGGCGGTTCTGGCGGAAATCGTCCGGAAGACGGGCGCGGGCGCCGTATTCTGGAATCGCCGCTACGATCCGGCGGGACG
CGAGGCCGATACCGCCATCAAGGCGGATCTCAGGGCGCGGGGCCTCACGGTCCGCAGTTTCCCCGGTGCCCTTCTACATG
AACCATGGAGCGTCTGTACGCGCTCGGTTGCCCCATACAAGATCTTTACCGCCTTCTGGCGCGCCGCCTGTGCGCTTCCG
GCGCCCTTTCCGCCCCATCCCGCGCCGGAAAGACTCGTCTTCGCCCCTCCCCCCCCGATGCCGGACAGCCTGATCACGCG
GCACGAGCAGGATGGATTGCTGCCGCGCCACCCGGACTGGGCGGGCGGACTGCGCGACATGTGGTCGCCGGGTGAGGAGG
AAGCGGGCGACCAGCTTTCCGATTTTCTCCGGCATGACGGGGCCGGCTACGCTACCGCCCGCGATTTCCCCGGGGATGAG
GCGACCTCCCGGCTGTCTCCCTTTCTGCGATTTGGCCATGTCAGCCCGGCACAGGTGTGGCACGCGGCCACGCGGAAGGC
CTGTCCGGACAAGTTCCTGATGGAACTCGGCTGGCGGGATTTCGCGTGGTCGCTCCTGTTCTTCAACCCGGATCTCGCGA
CCCGGAATCTGCGGCCGGAGTTCGACGCCATGCCCTGGCGTCACGATCCCGGGGGCCTTGCGGCGTGGCAGCGGGGCCAG
ACGGGATATCCCCTGGTCGATGCCGGAATGCGGGAACTCTGGCACACCGGGTGGATGCATAACCGCGTCCGCATGGTAGC
GGCGTCATTTTTGGTCAAGCACCTGCTGATCGACTGGCGGGAAGGCGAGCGCTGGTTTGCCGACACGCTGGTCGACCATG
ATCCGGCCAGCAACCCGATGAACTGGCAGTGGAACGCCGGCACAGGCGTGGATGCCGCGCCCTATTTCCGCGTCATGAAC
CCCATTCTGCAGTCCCGGAAATTCGATCCCCACGGCGTCTATATCCGCCGCTGGGTGCCCGAGCTTGCGCATCTGTCCGA
CGACGCCATCCACGCCCCATGGGAATCAGGCGCAACCCATCCCTATCCCGCGCCCATCGTGGATCATCGCGCAGCGCGAG
AACGGGCCCTGGCGGCCTGGAAGATGATCTGA

Upstream 100 bases:

>100_bases
GATGCGCCTGCGCGCCGCCCTCGAACGCCCCCAGGCCGACGACGTACGCGCCCGCGTGCGCGCCCTGCTGGGCGACCTGC
CCAAGTGACATCCGGGTAGG

Downstream 100 bases:

>100_bases
TACCAACGGTTATAGACACAGACCTGTGTGAGCCCACTTTCTGAGCCCGATGGATCGGATGGTGTCAGGCAGGGCGGCAA
GCTTGTTCCAGGCGGAACAG

Product: deoxyribodipyrimidine photo-lyase

Products: NA

Alternate protein names: DNA photolyase; Photoreactivating enzyme [H]

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MVNLAPAPTIVWFREDFRIADNMALLEAEKRGQPTLCIVILDDAHLPGAAARWWLHGAIRSLDETLRGMGGALHVFRGAA
RAVLAEIVRKTGAGAVFWNRRYDPAGREADTAIKADLRARGLTVRSFPGALLHEPWSVCTRSVAPYKIFTAFWRAACALP
APFPPHPAPERLVFAPPPPMPDSLITRHEQDGLLPRHPDWAGGLRDMWSPGEEEAGDQLSDFLRHDGAGYATARDFPGDE
ATSRLSPFLRFGHVSPAQVWHAATRKACPDKFLMELGWRDFAWSLLFFNPDLATRNLRPEFDAMPWRHDPGGLAAWQRGQ
TGYPLVDAGMRELWHTGWMHNRVRMVAASFLVKHLLIDWREGERWFADTLVDHDPASNPMNWQWNAGTGVDAAPYFRVMN
PILQSRKFDPHGVYIRRWVPELAHLSDDAIHAPWESGATHPYPAPIVDHRAARERALAAWKMI

Sequences:

>Translated_463_residues
MVNLAPAPTIVWFREDFRIADNMALLEAEKRGQPTLCIVILDDAHLPGAAARWWLHGAIRSLDETLRGMGGALHVFRGAA
RAVLAEIVRKTGAGAVFWNRRYDPAGREADTAIKADLRARGLTVRSFPGALLHEPWSVCTRSVAPYKIFTAFWRAACALP
APFPPHPAPERLVFAPPPPMPDSLITRHEQDGLLPRHPDWAGGLRDMWSPGEEEAGDQLSDFLRHDGAGYATARDFPGDE
ATSRLSPFLRFGHVSPAQVWHAATRKACPDKFLMELGWRDFAWSLLFFNPDLATRNLRPEFDAMPWRHDPGGLAAWQRGQ
TGYPLVDAGMRELWHTGWMHNRVRMVAASFLVKHLLIDWREGERWFADTLVDHDPASNPMNWQWNAGTGVDAAPYFRVMN
PILQSRKFDPHGVYIRRWVPELAHLSDDAIHAPWESGATHPYPAPIVDHRAARERALAAWKMI
>Mature_463_residues
MVNLAPAPTIVWFREDFRIADNMALLEAEKRGQPTLCIVILDDAHLPGAAARWWLHGAIRSLDETLRGMGGALHVFRGAA
RAVLAEIVRKTGAGAVFWNRRYDPAGREADTAIKADLRARGLTVRSFPGALLHEPWSVCTRSVAPYKIFTAFWRAACALP
APFPPHPAPERLVFAPPPPMPDSLITRHEQDGLLPRHPDWAGGLRDMWSPGEEEAGDQLSDFLRHDGAGYATARDFPGDE
ATSRLSPFLRFGHVSPAQVWHAATRKACPDKFLMELGWRDFAWSLLFFNPDLATRNLRPEFDAMPWRHDPGGLAAWQRGQ
TGYPLVDAGMRELWHTGWMHNRVRMVAASFLVKHLLIDWREGERWFADTLVDHDPASNPMNWQWNAGTGVDAAPYFRVMN
PILQSRKFDPHGVYIRRWVPELAHLSDDAIHAPWESGATHPYPAPIVDHRAARERALAAWKMI

Specific function: Involved in repair of UV radiation-induced DNA damage. Catalyzes the light-dependent monomerization (300-600 nm) of cyclobutyl pyrimidine dimers (in cis-syn configuration), which are formed between adjacent bases on the same DNA strand upon exposure to ul

COG id: COG0415

COG function: function code L; Deoxyribodipyrimidine photolyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DNA photolyase domain [H]

Homologues:

Organism=Homo sapiens, GI188536100, Length=505, Percent_Identity=26.1386138613861, Blast_Score=146, Evalue=4e-35,
Organism=Homo sapiens, GI4758072, Length=483, Percent_Identity=26.7080745341615, Blast_Score=142, Evalue=5e-34,
Organism=Homo sapiens, GI188536103, Length=461, Percent_Identity=25.5965292841649, Blast_Score=131, Evalue=2e-30,
Organism=Escherichia coli, GI1786926, Length=482, Percent_Identity=35.4771784232365, Blast_Score=258, Evalue=4e-70,
Organism=Saccharomyces cerevisiae, GI6324962, Length=497, Percent_Identity=28.3702213279678, Blast_Score=181, Evalue=2e-46,
Organism=Drosophila melanogaster, GI17137248, Length=470, Percent_Identity=28.7234042553192, Blast_Score=159, Evalue=4e-39,
Organism=Drosophila melanogaster, GI24585455, Length=470, Percent_Identity=28.7234042553192, Blast_Score=159, Evalue=4e-39,
Organism=Drosophila melanogaster, GI24648152, Length=511, Percent_Identity=23.679060665362, Blast_Score=117, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002081
- InterPro:   IPR018394
- InterPro:   IPR006050
- InterPro:   IPR005101
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00875 DNA_photolyase; PF03441 FAD_binding_7 [H]

EC number: =4.1.99.3 [H]

Molecular weight: Translated: 52143; Mature: 52143

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: PS00394 DNA_PHOTOLYASES_1_1 ; PS00691 DNA_PHOTOLYASES_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNLAPAPTIVWFREDFRIADNMALLEAEKRGQPTLCIVILDDAHLPGAAARWWLHGAIR
CCCCCCCCEEEEEECCHHHHCCHHHEEHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHH
SLDETLRGMGGALHVFRGAARAVLAEIVRKTGAGAVFWNRRYDPAGREADTAIKADLRAR
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHC
GLTVRSFPGALLHEPWSVCTRSVAPYKIFTAFWRAACALPAPFPPHPAPERLVFAPPPPM
CCEEECCCCHHHCCCHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCC
PDSLITRHEQDGLLPRHPDWAGGLRDMWSPGEEEAGDQLSDFLRHDGAGYATARDFPGDE
CHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCH
ATSRLSPFLRFGHVSPAQVWHAATRKACPDKFLMELGWRDFAWSLLFFNPDLATRNLRPE
HHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHCCCHHHEEEEEEECCCCCCCCCCCC
FDAMPWRHDPGGLAAWQRGQTGYPLVDAGMRELWHTGWMHNRVRMVAASFLVKHLLIDWR
CCCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EGERWFADTLVDHDPASNPMNWQWNAGTGVDAAPYFRVMNPILQSRKFDPHGVYIRRWVP
CCCHHHHHHHHCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHH
ELAHLSDDAIHAPWESGATHPYPAPIVDHRAARERALAAWKMI
HHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVNLAPAPTIVWFREDFRIADNMALLEAEKRGQPTLCIVILDDAHLPGAAARWWLHGAIR
CCCCCCCCEEEEEECCHHHHCCHHHEEHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHH
SLDETLRGMGGALHVFRGAARAVLAEIVRKTGAGAVFWNRRYDPAGREADTAIKADLRAR
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHC
GLTVRSFPGALLHEPWSVCTRSVAPYKIFTAFWRAACALPAPFPPHPAPERLVFAPPPPM
CCEEECCCCHHHCCCHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCC
PDSLITRHEQDGLLPRHPDWAGGLRDMWSPGEEEAGDQLSDFLRHDGAGYATARDFPGDE
CHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCH
ATSRLSPFLRFGHVSPAQVWHAATRKACPDKFLMELGWRDFAWSLLFFNPDLATRNLRPE
HHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHCCCHHHEEEEEEECCCCCCCCCCCC
FDAMPWRHDPGGLAAWQRGQTGYPLVDAGMRELWHTGWMHNRVRMVAASFLVKHLLIDWR
CCCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EGERWFADTLVDHDPASNPMNWQWNAGTGVDAAPYFRVMNPILQSRKFDPHGVYIRRWVP
CCCHHHHHHHHCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHH
ELAHLSDDAIHAPWESGATHPYPAPIVDHRAARERALAAWKMI
HHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7678007 [H]