BacMap

Definition	Gluconacetobacter diazotrophicus PAl 5 chromosome, complete genome.
Accession	NC_010125
Length	3,944,163

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The map label for this gene is dnaJ [H]

Identifier: 162146572

GI number: 162146572

Start: 766351

End: 767274

Strand: Direct

Name: dnaJ [H]

Synonym: GDI_0749

Alternate gene names: 162146572

Gene position: 766351-767274 (Clockwise)

Preceding gene: 162146571

Following gene: 162146573

Centisome position: 19.43

GC content: 69.91

Gene sequence:

>924_bases
GTGAGCACCGATCCATACGAAATCCTGGGCGTGTCGCGCACGGCCAGCCAGGACGATATTCGCAAGGCCTATCGCAAGCT
GGCCAAGGCCTGGCACCCCGACCTGAATCCGGGCGACAAGGCGGCGGAGGAAAAGTTCAAGGCGATCGGCACGGCGCATG
CGCTGCTGTCCGACGCGGAACAGCGCGCGCGCTTCGACCGGGGCGAGATCGATGCCGCCGGCCAGGAACGCGGCTGGGCG
CCCGGCGGGGGCGGTTATCGCGACCATGCCGAGGGCGCGCAGGGGTTTCGCTATTCCGCCGGCGGCGGCGGTTTTTCGGA
AGACGATCTGGGCGACATCTTCGGGTCGATGTTCGGCCAGCAGCGCGGCTACCGCCGCCGCCCCCAGGGGCCGGCGCGGG
GCGAGGACCGCAATTTCTCCCTGACCGTCAGCTTCGCCGATGCGGTCAATGGCGGCACGTCGCGCATTACCCTGCCGGGC
GGCGGCACGCTGGACGTCAAGATCCCTCCGGGCATCGAGGACGGTCAGGTCCTGCGCCTGCGGGGCAAAGGGGGCGAGGG
CCATCAGGGCGGCCCGGCGGGCGATGCGCTGATCACGATCAGCATCGCGCCCAGCCCGGATTACACGCGTGACGGCAACG
ATATCCGCATGACGCTGCCGGTGGGGCTGAAGGTCGCGGTGCTGGGCGGCTCGGTCACGGTCCCGACCCCGGGCGGCCCG
GTCTCGATGAACATTCCCGCCCATTCCGACACCGGCCGCCTGATGCGCCTGCGCGGGCGCGGGGTAAAGGCCCATGGGAA
GCGCGAGGCGGGCAATCTGTACGTCACGCTGAAGGTCGTGATCGGCCCGACCGACGCGGCGCTGGAAGACTTCCTGAAAT
CCTGGACCCCGCCGGAGGCGCCTGCCGGGGGAAGCACGACATGA

Upstream 100 bases:

>100_bases
ACCGTCCGGCGGGCACTCCCCATTATTTTCAGAGAAACAGGCGCTTCCATGGGGCGCCGAAGCCCGATTTCGCACCCAGG
CAGGGAGAAGACGCACCGTC

Downstream 100 bases:

>100_bases
TCACGATCGAAACTCTGTGCCTGCGCGTCGGTAACGTGACGGTCGCGGACGTCGAACACTGGATCGACGTCGCCTGGCTG
CGGCCGGAGGGCGAGCCCGG

Product: chaperone protein DnaJ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MSTDPYEILGVSRTASQDDIRKAYRKLAKAWHPDLNPGDKAAEEKFKAIGTAHALLSDAEQRARFDRGEIDAAGQERGWA
PGGGGYRDHAEGAQGFRYSAGGGGFSEDDLGDIFGSMFGQQRGYRRRPQGPARGEDRNFSLTVSFADAVNGGTSRITLPG
GGTLDVKIPPGIEDGQVLRLRGKGGEGHQGGPAGDALITISIAPSPDYTRDGNDIRMTLPVGLKVAVLGGSVTVPTPGGP
VSMNIPAHSDTGRLMRLRGRGVKAHGKREAGNLYVTLKVVIGPTDAALEDFLKSWTPPEAPAGGSTT

Sequences:

>Translated_307_residues
MSTDPYEILGVSRTASQDDIRKAYRKLAKAWHPDLNPGDKAAEEKFKAIGTAHALLSDAEQRARFDRGEIDAAGQERGWA
PGGGGYRDHAEGAQGFRYSAGGGGFSEDDLGDIFGSMFGQQRGYRRRPQGPARGEDRNFSLTVSFADAVNGGTSRITLPG
GGTLDVKIPPGIEDGQVLRLRGKGGEGHQGGPAGDALITISIAPSPDYTRDGNDIRMTLPVGLKVAVLGGSVTVPTPGGP
VSMNIPAHSDTGRLMRLRGRGVKAHGKREAGNLYVTLKVVIGPTDAALEDFLKSWTPPEAPAGGSTT
>Mature_306_residues
STDPYEILGVSRTASQDDIRKAYRKLAKAWHPDLNPGDKAAEEKFKAIGTAHALLSDAEQRARFDRGEIDAAGQERGWAP
GGGGYRDHAEGAQGFRYSAGGGGFSEDDLGDIFGSMFGQQRGYRRRPQGPARGEDRNFSLTVSFADAVNGGTSRITLPGG
GTLDVKIPPGIEDGQVLRLRGKGGEGHQGGPAGDALITISIAPSPDYTRDGNDIRMTLPVGLKVAVLGGSVTVPTPGGPV
SMNIPAHSDTGRLMRLRGRGVKAHGKREAGNLYVTLKVVIGPTDAALEDFLKSWTPPEAPAGGSTT

Specific function: DNA-binding protein that preferentially recognizes a curved DNA sequence. It is probably a functional analog of DnaJ; displays overlapping activities with DnaJ, but functions under different conditions, probably acting as a molecular chaperone in an adapt

COG id: COG2214

COG function: function code O; DnaJ-class molecular chaperone

Gene ontology:

Cell location: Cytoplasm, nucleoid [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 J domain [H]

Homologues:

Organism=Homo sapiens, GI205360838, Length=346, Percent_Identity=26.878612716763, Blast_Score=102, Evalue=7e-22,
Organism=Homo sapiens, GI205360840, Length=346, Percent_Identity=26.878612716763, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI7706495, Length=262, Percent_Identity=31.2977099236641, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI6631085, Length=284, Percent_Identity=31.3380281690141, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI23503241, Length=115, Percent_Identity=40.8695652173913, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI5453690, Length=285, Percent_Identity=27.719298245614, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI201862587, Length=332, Percent_Identity=30.1204819277108, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI56687498, Length=67, Percent_Identity=55.2238805970149, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI56549115, Length=332, Percent_Identity=30.1204819277108, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI201862518, Length=332, Percent_Identity=30.1204819277108, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI4885495, Length=66, Percent_Identity=56.0606060606061, Blast_Score=79, Evalue=7e-15,
Organism=Homo sapiens, GI47777312, Length=121, Percent_Identity=43.801652892562, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI17388799, Length=66, Percent_Identity=56.0606060606061, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI29126218, Length=62, Percent_Identity=54.8387096774194, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI88501736, Length=63, Percent_Identity=55.5555555555556, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI27151736, Length=63, Percent_Identity=55.5555555555556, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI194306642, Length=68, Percent_Identity=57.3529411764706, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI194306640, Length=68, Percent_Identity=57.3529411764706, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI45504382, Length=62, Percent_Identity=50, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI21553335, Length=120, Percent_Identity=37.5, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI4504511, Length=164, Percent_Identity=33.5365853658537, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI194328760, Length=158, Percent_Identity=33.5443037974684, Blast_Score=69, Evalue=8e-12,
Organism=Homo sapiens, GI194328758, Length=158, Percent_Identity=33.5443037974684, Blast_Score=68, Evalue=8e-12,
Organism=Homo sapiens, GI22749377, Length=63, Percent_Identity=57.1428571428571, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI5031741, Length=161, Percent_Identity=35.4037267080745, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI68077166, Length=63, Percent_Identity=55.5555555555556, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI68077168, Length=63, Percent_Identity=55.5555555555556, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1787235, Length=294, Percent_Identity=34.3537414965986, Blast_Score=155, Evalue=4e-39,
Organism=Escherichia coli, GI1786197, Length=293, Percent_Identity=32.0819112627986, Blast_Score=103, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17553096, Length=351, Percent_Identity=27.0655270655271, Blast_Score=91, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17553098, Length=351, Percent_Identity=27.0655270655271, Blast_Score=91, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17534355, Length=309, Percent_Identity=29.7734627831715, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI212645553, Length=64, Percent_Identity=53.125, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI86563276, Length=120, Percent_Identity=40, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17554644, Length=117, Percent_Identity=34.1880341880342, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI115532476, Length=94, Percent_Identity=40.4255319148936, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17538236, Length=122, Percent_Identity=38.5245901639344, Blast_Score=68, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6324265, Length=158, Percent_Identity=35.4430379746835, Blast_Score=76, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6324321, Length=335, Percent_Identity=26.2686567164179, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6322194, Length=107, Percent_Identity=41.1214953271028, Blast_Score=71, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323813, Length=121, Percent_Identity=43.801652892562, Blast_Score=67, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6323870, Length=72, Percent_Identity=38.8888888888889, Blast_Score=63, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24658562, Length=286, Percent_Identity=31.1188811188811, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24658555, Length=286, Percent_Identity=31.1188811188811, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI18858081, Length=285, Percent_Identity=29.1228070175439, Blast_Score=88, Evalue=6e-18,
Organism=Drosophila melanogaster, GI85726398, Length=291, Percent_Identity=28.8659793814433, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI17737735, Length=62, Percent_Identity=51.6129032258064, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24668523, Length=62, Percent_Identity=51.6129032258064, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI28574723, Length=62, Percent_Identity=51.6129032258064, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24584630, Length=80, Percent_Identity=46.25, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI21355911, Length=79, Percent_Identity=48.1012658227848, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI17648033, Length=80, Percent_Identity=46.25, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24646562, Length=63, Percent_Identity=52.3809523809524, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24646560, Length=63, Percent_Identity=52.3809523809524, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24646558, Length=63, Percent_Identity=52.3809523809524, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24646556, Length=63, Percent_Identity=52.3809523809524, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI21357547, Length=63, Percent_Identity=52.3809523809524, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI21358273, Length=65, Percent_Identity=53.8461538461538, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI161077138, Length=65, Percent_Identity=46.1538461538462, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI45552811, Length=263, Percent_Identity=25.0950570342205, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI45552813, Length=263, Percent_Identity=25.0950570342205, Blast_Score=66, Evalue=4e-11,
Organism=Drosophila melanogaster, GI45549272, Length=263, Percent_Identity=25.0950570342205, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24654066, Length=65, Percent_Identity=44.6153846153846, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24654070, Length=65, Percent_Identity=44.6153846153846, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24654068, Length=65, Percent_Identity=44.6153846153846, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24654072, Length=65, Percent_Identity=44.6153846153846, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI19920464, Length=65, Percent_Identity=43.0769230769231, Blast_Score=65, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002939
- InterPro:   IPR001623
- InterPro:   IPR018253
- InterPro:   IPR008971
- InterPro:   IPR015609
- InterPro:   IPR003095 [H]

Pfam domain/function: PF00226 DnaJ; PF01556 DnaJ_C [H]

EC number: NA

Molecular weight: Translated: 32229; Mature: 32098

Theoretical pI: Translated: 8.77; Mature: 8.77

Prosite motif: PS00636 DNAJ_1 ; PS50076 DNAJ_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTDPYEILGVSRTASQDDIRKAYRKLAKAWHPDLNPGDKAAEEKFKAIGTAHALLSDAE
CCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
QRARFDRGEIDAAGQERGWAPGGGGYRDHAEGAQGFRYSAGGGGFSEDDLGDIFGSMFGQ
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHH
QRGYRRRPQGPARGEDRNFSLTVSFADAVNGGTSRITLPGGGTLDVKIPPGIEDGQVLRL
HCCCCCCCCCCCCCCCCCEEEEEEEHHCCCCCCCEEEECCCCEEEEEECCCCCCCCEEEE
RGKGGEGHQGGPAGDALITISIAPSPDYTRDGNDIRMTLPVGLKVAVLGGSVTVPTPGGP
ECCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCEEEEECCCEEEEEECCEEEECCCCCC
VSMNIPAHSDTGRLMRLRGRGVKAHGKREAGNLYVTLKVVIGPTDAALEDFLKSWTPPEA
EEEECCCCCCCCCEEEEECCCCCCCCCCCCCCEEEEEEEEECCCHHHHHHHHHHCCCCCC
PAGGSTT
CCCCCCC
>Mature Secondary Structure 
STDPYEILGVSRTASQDDIRKAYRKLAKAWHPDLNPGDKAAEEKFKAIGTAHALLSDAE
CCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
QRARFDRGEIDAAGQERGWAPGGGGYRDHAEGAQGFRYSAGGGGFSEDDLGDIFGSMFGQ
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHH
QRGYRRRPQGPARGEDRNFSLTVSFADAVNGGTSRITLPGGGTLDVKIPPGIEDGQVLRL
HCCCCCCCCCCCCCCCCCEEEEEEEHHCCCCCCCEEEECCCCEEEEEECCCCCCCCEEEE
RGKGGEGHQGGPAGDALITISIAPSPDYTRDGNDIRMTLPVGLKVAVLGGSVTVPTPGGP
ECCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCEEEEECCCEEEEEECCEEEECCCCCC
VSMNIPAHSDTGRLMRLRGRGVKAHGKREAGNLYVTLKVVIGPTDAALEDFLKSWTPPEA
EEEECCCCCCCCCEEEEECCCCCCCCCCCCCCEEEEEEEEECCCHHHHHHHHHHCCCCCC
PAGGSTT
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA