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The map label for this gene is dadB [H]

Identifier: 162146534

GI number: 162146534

Start: 721767

End: 722930

Strand: Reverse

Name: dadB [H]

Synonym: GDI_0710

Alternate gene names: 162146534

Gene position: 722930-721767 (Counterclockwise)

Preceding gene: 162146535

Following gene: 162146533

Centisome position: 18.33

GC content: 71.74

Gene sequence:

>1164_bases
ATGCTGACCGCGGACCCGATGCCCGCCGCCCCTTCGGCCTGGACGGGGGGCATCCTGACGATCGATCTGGACGCGGTCGC
CGCCAATTACCGCATCCTGCGCGACCGGGTCGGTCCCCGCACGATATGCGGCGCGGCGGTCAAGGCCGATGCGTACGGGC
TGGGCGCGGCCCGCATCGTGCCCGTCCTGGCGGAAGCCGGGTGCCATACCTTCTTCGTCGCGCACCTGGCCGAGGGGGCT
GCCATCCGGCCCCTGCTGGGCACGGATGCCACGATTTTCGTCCTGAACGGCGCCATGCCGGGCACCGAAGCCGCGTTGCA
CGAACACGGACTGGTGCCGGTGCTGAACAGCCTGGAACAGATCGCGCGCTGGCGCGACCTGGCCCGGCAGGCCGGCAGGG
ACCTGCCTGCCGCGCTGCAGGTCGATAGCGGCATGTCACGCTTCGGCCTGTCGGACGCGGATGTGGATGCCCTGGCGGCG
CGGGACGGTTCCCTGGCCGGGATCACGCCGCTGCTGGTCATGAGCCACCTGGCCTGCGCCGACGAACCCGCCCACCCCGC
CAACCACGCGCAGCGCGCGGCCTTCCTGCGCCTGCGGGCCAGGCTGCCGGCGGCGCCCGCCAGCCTGGCCGCGTCGTCCG
GCCTGTTCATGGGCAGCGACTGGCATTTCGACATGGTGCGCCCGGGCGCCGCGCTGTACGGCATCAATCCCACGCCGGGC
TGGCCGAATCCGATGCAGCCGGTGGTCCGCCTGCAGGCGCGCATCGTGCAGACGCGCTGGATCGGCCCCGGGACGGCAGT
CGGATACGGCGCAGCCTTCATCGCCGACCGGCCCAGCCGGATCGCGACGCTGGCGGTGGGGTACGGCGACGGATTTCCCC
GCGCCGCGGGCGGCCACGGCCATGCGGTCCTGCCCGAACGGCCGGCGGCGAAGCTGCCGATCATCGGCCGGATTTCCATG
GATTGCCTGGCGGTGGACGTAACCGACATCAGCGACGCCCCCCTGGACGCCGGCACGGCGCTGGACCTGATCGGTCCGCA
TCATTCCATCGATTCCGCCGCCGCTGCCGCCGGAACCATCGGCTACGAACTGCTGACGGGGCTGGGCGCACGGTATCATC
GTCTCTATCGCGACGACCGGTCGCGGAAAGGATGCAACGGGTGA

Upstream 100 bases:

>100_bases
CTGTCGGGTGAATTTGTTATATCATCGCTGACCCCCATCCGGAGTCCGCGATGCGTTCCAGCCCGCGCCAGACGATGCCG
TACCGGCGGTCCTGCCCGCC

Downstream 100 bases:

>100_bases
AGGTCATCGTTCTGGGGGCCGGGGTCATCGGTGTCACCTCGGCCTGGTATCTGGCGAAACTGGGCCATGAGGTCGAAGTG
GTCGACCGGCAGCCCGCGGC

Product: alanine racemase, catabolic

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MLTADPMPAAPSAWTGGILTIDLDAVAANYRILRDRVGPRTICGAAVKADAYGLGAARIVPVLAEAGCHTFFVAHLAEGA
AIRPLLGTDATIFVLNGAMPGTEAALHEHGLVPVLNSLEQIARWRDLARQAGRDLPAALQVDSGMSRFGLSDADVDALAA
RDGSLAGITPLLVMSHLACADEPAHPANHAQRAAFLRLRARLPAAPASLAASSGLFMGSDWHFDMVRPGAALYGINPTPG
WPNPMQPVVRLQARIVQTRWIGPGTAVGYGAAFIADRPSRIATLAVGYGDGFPRAAGGHGHAVLPERPAAKLPIIGRISM
DCLAVDVTDISDAPLDAGTALDLIGPHHSIDSAAAAAGTIGYELLTGLGARYHRLYRDDRSRKGCNG

Sequences:

>Translated_387_residues
MLTADPMPAAPSAWTGGILTIDLDAVAANYRILRDRVGPRTICGAAVKADAYGLGAARIVPVLAEAGCHTFFVAHLAEGA
AIRPLLGTDATIFVLNGAMPGTEAALHEHGLVPVLNSLEQIARWRDLARQAGRDLPAALQVDSGMSRFGLSDADVDALAA
RDGSLAGITPLLVMSHLACADEPAHPANHAQRAAFLRLRARLPAAPASLAASSGLFMGSDWHFDMVRPGAALYGINPTPG
WPNPMQPVVRLQARIVQTRWIGPGTAVGYGAAFIADRPSRIATLAVGYGDGFPRAAGGHGHAVLPERPAAKLPIIGRISM
DCLAVDVTDISDAPLDAGTALDLIGPHHSIDSAAAAAGTIGYELLTGLGARYHRLYRDDRSRKGCNG
>Mature_387_residues
MLTADPMPAAPSAWTGGILTIDLDAVAANYRILRDRVGPRTICGAAVKADAYGLGAARIVPVLAEAGCHTFFVAHLAEGA
AIRPLLGTDATIFVLNGAMPGTEAALHEHGLVPVLNSLEQIARWRDLARQAGRDLPAALQVDSGMSRFGLSDADVDALAA
RDGSLAGITPLLVMSHLACADEPAHPANHAQRAAFLRLRARLPAAPASLAASSGLFMGSDWHFDMVRPGAALYGINPTPG
WPNPMQPVVRLQARIVQTRWIGPGTAVGYGAAFIADRPSRIATLAVGYGDGFPRAAGGHGHAVLPERPAAKLPIIGRISM
DCLAVDVTDISDAPLDAGTALDLIGPHHSIDSAAAAAGTIGYELLTGLGARYHRLYRDDRSRKGCNG

Specific function: Isomerizes L-alanine to D-alanine which is then oxidized to pyruvate by DadA [H]

COG id: COG0787

COG function: function code M; Alanine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alanine racemase family [H]

Homologues:

Organism=Escherichia coli, GI1787439, Length=357, Percent_Identity=31.6526610644258, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI1790487, Length=371, Percent_Identity=31.8059299191375, Blast_Score=135, Evalue=3e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000821
- InterPro:   IPR009006
- InterPro:   IPR011079
- InterPro:   IPR001608
- InterPro:   IPR020622 [H]

Pfam domain/function: PF00842 Ala_racemase_C; PF01168 Ala_racemase_N [H]

EC number: =5.1.1.1 [H]

Molecular weight: Translated: 40296; Mature: 40296

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: PS00395 ALANINE_RACEMASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTADPMPAAPSAWTGGILTIDLDAVAANYRILRDRVGPRTICGAAVKADAYGLGAARIV
CCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHH
PVLAEAGCHTFFVAHLAEGAAIRPLLGTDATIFVLNGAMPGTEAALHEHGLVPVLNSLEQ
HHHHHCCHHHHHHHHHHCCCEECEECCCCEEEEEEECCCCCHHHHHHHCCCHHHHHHHHH
IARWRDLARQAGRDLPAALQVDSGMSRFGLSDADVDALAARDGSLAGITPLLVMSHLACA
HHHHHHHHHHHCCCCCEEEEECCCHHHCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHC
DEPAHPANHAQRAAFLRLRARLPAAPASLAASSGLFMGSDWHFDMVRPGAALYGINPTPG
CCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHCCCCEECCCCCEEEECCCCEEEECCCCCC
WPNPMQPVVRLQARIVQTRWIGPGTAVGYGAAFIADRPSRIATLAVGYGDGFPRAAGGHG
CCCHHHHHHHHHHHHHHHEECCCCCHHHHCCCEECCCCCCEEEEEEECCCCCCCCCCCCC
HAVLPERPAAKLPIIGRISMDCLAVDVTDISDAPLDAGTALDLIGPHHSIDSAAAAAGTI
CCCCCCCCCCCCCEEEEECCEEEEEEECCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHH
GYELLTGLGARYHRLYRDDRSRKGCNG
HHHHHHHCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MLTADPMPAAPSAWTGGILTIDLDAVAANYRILRDRVGPRTICGAAVKADAYGLGAARIV
CCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHH
PVLAEAGCHTFFVAHLAEGAAIRPLLGTDATIFVLNGAMPGTEAALHEHGLVPVLNSLEQ
HHHHHCCHHHHHHHHHHCCCEECEECCCCEEEEEEECCCCCHHHHHHHCCCHHHHHHHHH
IARWRDLARQAGRDLPAALQVDSGMSRFGLSDADVDALAARDGSLAGITPLLVMSHLACA
HHHHHHHHHHHCCCCCEEEEECCCHHHCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHC
DEPAHPANHAQRAAFLRLRARLPAAPASLAASSGLFMGSDWHFDMVRPGAALYGINPTPG
CCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHCCCCEECCCCCEEEECCCCEEEECCCCCC
WPNPMQPVVRLQARIVQTRWIGPGTAVGYGAAFIADRPSRIATLAVGYGDGFPRAAGGHG
CCCHHHHHHHHHHHHHHHEECCCCCHHHHCCCEECCCCCCEEEEEEECCCCCCCCCCCCC
HAVLPERPAAKLPIIGRISMDCLAVDVTDISDAPLDAGTALDLIGPHHSIDSAAAAAGTI
CCCCCCCCCCCCCEEEEECCEEEEEEECCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHH
GYELLTGLGARYHRLYRDDRSRKGCNG
HHHHHHHCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]