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The map label for this gene is irlS [H]

Identifier: 162146498

GI number: 162146498

Start: 680985

End: 682424

Strand: Direct

Name: irlS [H]

Synonym: GDI_0674

Alternate gene names: 162146498

Gene position: 680985-682424 (Clockwise)

Preceding gene: 162146497

Following gene: 162146499

Centisome position: 17.27

GC content: 68.12

Gene sequence:

>1440_bases
ATGTCCCCGGCCGGCAGGGGCCGGTTCCGCACCTTGCGGCGGCTCACGTCCAGCCTGTCCTTCCGGATCGTCATCCTGTT
CGCGGGGTTCCTGATGCTGTCGGGCATCCTGGTGATCCTGCTGTCGGGATGGCGGAGCCAGCGTTCGCTGGAAGGCCAGA
TCCGCCGTGCCGCCACGACGGAACAGGCGGAAGCCCTGTCGGCGGCCGGCACGCGGGACCTGGAGCATCTTCTGCCGGTC
GTGCGGGACCTGGTGCGCCATGAACCGGATTTCTTCTATCTGCTGCAGGATGGTTCGGGCCACGTCATGGCGGGCAACAT
GCTGAATCTGCGCCCCGTGCCCGGCAGTCGCTGGCTGTCGCGGGCGCATCGGCGGCCCTTCGGCGAGGCGAACGGCCTGG
TGTACGGCCAGGGCCTGGTCCTGGTCGATGGCGGTTATTTCTTCGTGGGGATCGATGCATCGCCCCTGCGCCGGCTGCGG
CATGAATTCCGGATCGTCGTGGGCTGGAGTCTCGTGGGGTTCGCCGTGCTGGGCGTGGGGGGCGGCCTGATCCTCAGCGC
CATCATCCTGGGCCGGATCGAGACGATCGGCGCGATCGCGCGGCGGATCATGAACGGCGATCTCTCGCAGCGGATTCCCC
ATCAGGGGGCGAATGACGAATTCGATGACCTGGCCCACGCCCTCAACGCGATGCTGGAGCGCAACGAACGGCTGATCGCC
AGCCTGCAGCAGGTCTCGAACGACATCGCGCACGACATGCGCCGCCCGCTCTCGCGCCTGCGACAGCGGCTGGAAGCGGC
ATCGGTCGCCGACCACACGCCCGATGCGTTCCGCGCCACCATCGAGGACGCGGTCGAGGACCTGGATTCGGCGCTGGAAA
TCTTCTCGTCGCTGCTGCGGCTGTCGCAGATCGAGGCCGCGATTTCGACCGGCACCGTCATGCCCGTCGCCGTGGGCGGT
CTGGTCAGGGAGGTCGTGGACCTTTTTCGTCCGGTGGCCGAGGACCGGGGACAGGCCCTGGCCGTCCGCTCCCTCGATTC
CGGTCCGGTTTCCGGGACCGTCATGGGCGATCCGGTCCTGCTGATGCAGATGCTGTCGAACCTGGTGGAAAACGCGATCA
ACCACACGCCGCATGGCAGCCACATCACCGTCGGGGCCACGCTGTCGGGCGACCGGATCATGCTGGTGGTGGCCGATGAC
GGGGCCGGCATTCCGCCCGACAGCCGCGAACGGGTCTTCCACCGTTTCGTCCGGCTGGACGACAGCCGGTCGGTGCCCGG
CAGCGGGCTGGGCCTCAGCCTGGTGCGGGCGATCGTGCGGCTGCATGGCGGCACGATCGCCCTGTCGGACAACCGGCCCG
GTTTGCGTTGCACCGTCATCCTGCCGCATATCGCCTCGATTGACCCAACCGACGGGCCGACTCGCCTGGGGGACAGATAG

Upstream 100 bases:

>100_bases
GCCTGCGCGACAAGCTGGGGCCGGATGGGCGGCAGGTGATCCAGACCATTCGTGGCGCCGGCTACGTCATGGGCGGCGTG
GCGGGCGCGGAATCGTGATG

Downstream 100 bases:

>100_bases
TGACCGATACTGCCGAGATCCTGGCTTCTCCCGCGCCCGCCGTTGCCGATGGGGGGCGGCCCCTGGCGGTCGTGACCGGC
GGGACGGGCGGGATCGGGCG

Product: two-component sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MSPAGRGRFRTLRRLTSSLSFRIVILFAGFLMLSGILVILLSGWRSQRSLEGQIRRAATTEQAEALSAAGTRDLEHLLPV
VRDLVRHEPDFFYLLQDGSGHVMAGNMLNLRPVPGSRWLSRAHRRPFGEANGLVYGQGLVLVDGGYFFVGIDASPLRRLR
HEFRIVVGWSLVGFAVLGVGGGLILSAIILGRIETIGAIARRIMNGDLSQRIPHQGANDEFDDLAHALNAMLERNERLIA
SLQQVSNDIAHDMRRPLSRLRQRLEAASVADHTPDAFRATIEDAVEDLDSALEIFSSLLRLSQIEAAISTGTVMPVAVGG
LVREVVDLFRPVAEDRGQALAVRSLDSGPVSGTVMGDPVLLMQMLSNLVENAINHTPHGSHITVGATLSGDRIMLVVADD
GAGIPPDSRERVFHRFVRLDDSRSVPGSGLGLSLVRAIVRLHGGTIALSDNRPGLRCTVILPHIASIDPTDGPTRLGDR

Sequences:

>Translated_479_residues
MSPAGRGRFRTLRRLTSSLSFRIVILFAGFLMLSGILVILLSGWRSQRSLEGQIRRAATTEQAEALSAAGTRDLEHLLPV
VRDLVRHEPDFFYLLQDGSGHVMAGNMLNLRPVPGSRWLSRAHRRPFGEANGLVYGQGLVLVDGGYFFVGIDASPLRRLR
HEFRIVVGWSLVGFAVLGVGGGLILSAIILGRIETIGAIARRIMNGDLSQRIPHQGANDEFDDLAHALNAMLERNERLIA
SLQQVSNDIAHDMRRPLSRLRQRLEAASVADHTPDAFRATIEDAVEDLDSALEIFSSLLRLSQIEAAISTGTVMPVAVGG
LVREVVDLFRPVAEDRGQALAVRSLDSGPVSGTVMGDPVLLMQMLSNLVENAINHTPHGSHITVGATLSGDRIMLVVADD
GAGIPPDSRERVFHRFVRLDDSRSVPGSGLGLSLVRAIVRLHGGTIALSDNRPGLRCTVILPHIASIDPTDGPTRLGDR
>Mature_478_residues
SPAGRGRFRTLRRLTSSLSFRIVILFAGFLMLSGILVILLSGWRSQRSLEGQIRRAATTEQAEALSAAGTRDLEHLLPVV
RDLVRHEPDFFYLLQDGSGHVMAGNMLNLRPVPGSRWLSRAHRRPFGEANGLVYGQGLVLVDGGYFFVGIDASPLRRLRH
EFRIVVGWSLVGFAVLGVGGGLILSAIILGRIETIGAIARRIMNGDLSQRIPHQGANDEFDDLAHALNAMLERNERLIAS
LQQVSNDIAHDMRRPLSRLRQRLEAASVADHTPDAFRATIEDAVEDLDSALEIFSSLLRLSQIEAAISTGTVMPVAVGGL
VREVVDLFRPVAEDRGQALAVRSLDSGPVSGTVMGDPVLLMQMLSNLVENAINHTPHGSHITVGATLSGDRIMLVVADDG
AGIPPDSRERVFHRFVRLDDSRSVPGSGLGLSLVRAIVRLHGGTIALSDNRPGLRCTVILPHIASIDPTDGPTRLGDR

Specific function: Member of the two-component regulatory system irlR/irlS. May be involved in invasion of eukaryotic cells and heavy-metal resistance. Probably activates irlR by phosphorylation [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1786783, Length=272, Percent_Identity=31.6176470588235, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI1787894, Length=263, Percent_Identity=32.6996197718631, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1790346, Length=268, Percent_Identity=30.9701492537313, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1789403, Length=312, Percent_Identity=27.5641025641026, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1788279, Length=227, Percent_Identity=27.7533039647577, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1788393, Length=270, Percent_Identity=30.3703703703704, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1790861, Length=246, Percent_Identity=30.4878048780488, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1789808, Length=235, Percent_Identity=28.936170212766, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1790551, Length=247, Percent_Identity=31.1740890688259, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1786600, Length=218, Percent_Identity=30.7339449541284, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1786912, Length=239, Percent_Identity=26.3598326359833, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI87082128, Length=290, Percent_Identity=24.4827586206897, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI1790436, Length=246, Percent_Identity=28.8617886178862, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1789149, Length=214, Percent_Identity=31.3084112149533, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1788713, Length=248, Percent_Identity=26.2096774193548, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR006290
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 51933; Mature: 51801

Theoretical pI: Translated: 8.00; Mature: 8.00

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPAGRGRFRTLRRLTSSLSFRIVILFAGFLMLSGILVILLSGWRSQRSLEGQIRRAATT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
EQAEALSAAGTRDLEHLLPVVRDLVRHEPDFFYLLQDGSGHVMAGNMLNLRPVPGSRWLS
HHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEECCEEEEECCCCHHHHH
RAHRRPFGEANGLVYGQGLVLVDGGYFFVGIDASPLRRLRHEFRIVVGWSLVGFAVLGVG
HHHCCCCCCCCCEEEECCEEEEECCEEEEECCHHHHHHHHHHHEEEEHHHHHHHHHHHCC
GGLILSAIILGRIETIGAIARRIMNGDLSQRIPHQGANDEFDDLAHALNAMLERNERLIA
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
SLQQVSNDIAHDMRRPLSRLRQRLEAASVADHTPDAFRATIEDAVEDLDSALEIFSSLLR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LSQIEAAISTGTVMPVAVGGLVREVVDLFRPVAEDRGQALAVRSLDSGPVSGTVMGDPVL
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEECCCHHH
LMQMLSNLVENAINHTPHGSHITVGATLSGDRIMLVVADDGAGIPPDSRERVFHRFVRLD
HHHHHHHHHHHHHCCCCCCCEEEEEEEECCCEEEEEEECCCCCCCCCHHHHHHHHHHHCC
DSRSVPGSGLGLSLVRAIVRLHGGTIALSDNRPGLRCTVILPHIASIDPTDGPTRLGDR
CCCCCCCCCCCHHHHHHHHHHCCCEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SPAGRGRFRTLRRLTSSLSFRIVILFAGFLMLSGILVILLSGWRSQRSLEGQIRRAATT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
EQAEALSAAGTRDLEHLLPVVRDLVRHEPDFFYLLQDGSGHVMAGNMLNLRPVPGSRWLS
HHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEECCEEEEECCCCHHHHH
RAHRRPFGEANGLVYGQGLVLVDGGYFFVGIDASPLRRLRHEFRIVVGWSLVGFAVLGVG
HHHCCCCCCCCCEEEECCEEEEECCEEEEECCHHHHHHHHHHHEEEEHHHHHHHHHHHCC
GGLILSAIILGRIETIGAIARRIMNGDLSQRIPHQGANDEFDDLAHALNAMLERNERLIA
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
SLQQVSNDIAHDMRRPLSRLRQRLEAASVADHTPDAFRATIEDAVEDLDSALEIFSSLLR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LSQIEAAISTGTVMPVAVGGLVREVVDLFRPVAEDRGQALAVRSLDSGPVSGTVMGDPVL
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEECCCHHH
LMQMLSNLVENAINHTPHGSHITVGATLSGDRIMLVVADDGAGIPPDSRERVFHRFVRLD
HHHHHHHHHHHHHCCCCCCCEEEEEEEECCCEEEEEEECCCCCCCCCHHHHHHHHHHHCC
DSRSVPGSGLGLSLVRAIVRLHGGTIALSDNRPGLRCTVILPHIASIDPTDGPTRLGDR
CCCCCCCCCCCHHHHHHHHHHCCCEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9393784 [H]