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The map label for this gene is yeiE [H]

Identifier: 162146483

GI number: 162146483

Start: 667880

End: 668779

Strand: Reverse

Name: yeiE [H]

Synonym: GDI_0659

Alternate gene names: 162146483

Gene position: 668779-667880 (Counterclockwise)

Preceding gene: 162146487

Following gene: 162146481

Centisome position: 16.96

GC content: 68.11

Gene sequence:

>900_bases
ATGACCCTGGAACAACTCCGCCTCTTTATCGCCGTCGCGGAACGGGAACACGTCACCGCCGCCAGCCGCGCCCTGAACGT
GACCCAGTCCGCCGTATCGGCCGCCATCGCGACGCTGGAAGAACGGCATGGCGTGAAGCTCTTCCACCGTGTCGGCCGGG
GCATTCGCCTGACGGACGCCGGCCGGCTGTTCCTGCCCGAGGCCCGTGCTGTCCTGGCCCGGGCCGTGGCGGCCGAAAAC
GTGCTGGACGATTTCAGCGGCCTGAAGCGCGGCACATTGCGTGTCGTGGCCAGCCAGACCATCGCCGCGTATTGGCTGCC
CCCCATCCTGGTATCGTTTCGCAAGCAATATCCAGGGATTACGATCGACCTGGCGATCAGCAATACCGAAGACGCAGCCG
CCAGGGTCAGGGACGGAGAGGTCGAACTGGGCATCGTCGAGGCGATCGTCGACGACCCCGCACTGGCGCAATGGCCGCTG
GGGGAAGATCGCCTGACCATCATCCAGGCCGATCCGTGCCTGCCCCGGACGGTCGACCGGGCATGGCTACGGGCCGCCCG
CTGGGTGATGCGGGAGCCCGGATCGGGTACGCGCTCGACGCTGGAGCAGCACCTCCGTCACCTCGGGATCGAACCCGAAG
ACCTGACCATCAGTCTTGTCCTGCCCTCCAACGAAAGCGTGCGGACGGCGATCGAAGCGGGGGCCGGGATCGGGGCCCTG
TCCTCGCTGGTCGTGGCCCCGGCGTTACAGGCCGGCACGGTGCACGCCGTGCCGCAGGCGCTGGGCGCGCGGTCGTTCTA
CGGGTTGCGACATAAGGAGCGCTATCGCAGCCCGGCCGCCGAGGCCCTGCTGAAGCTTATCGGCCAGGAGCCGCCGGGAA
CGCCGAACCCGGTAGGTTGA

Upstream 100 bases:

>100_bases
CCTTCGCCACGAAGACTAGGAGGCAGCCATCTCATCACTCCAACTCATAATTTTTCTGATTCCGATCGGAAATCATGATT
GGTTGTGATATCCGGCGGGC

Downstream 100 bases:

>100_bases
GTCACACGGGATCGCACTCCCGCGCATGGTCCTTCGCCCTTACGACGCGCCCGTACGCATGCTGGCCGAACTCGGGCGCA
TATCGGTCATCCGCCCAGGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MTLEQLRLFIAVAEREHVTAASRALNVTQSAVSAAIATLEERHGVKLFHRVGRGIRLTDAGRLFLPEARAVLARAVAAEN
VLDDFSGLKRGTLRVVASQTIAAYWLPPILVSFRKQYPGITIDLAISNTEDAAARVRDGEVELGIVEAIVDDPALAQWPL
GEDRLTIIQADPCLPRTVDRAWLRAARWVMREPGSGTRSTLEQHLRHLGIEPEDLTISLVLPSNESVRTAIEAGAGIGAL
SSLVVAPALQAGTVHAVPQALGARSFYGLRHKERYRSPAAEALLKLIGQEPPGTPNPVG

Sequences:

>Translated_299_residues
MTLEQLRLFIAVAEREHVTAASRALNVTQSAVSAAIATLEERHGVKLFHRVGRGIRLTDAGRLFLPEARAVLARAVAAEN
VLDDFSGLKRGTLRVVASQTIAAYWLPPILVSFRKQYPGITIDLAISNTEDAAARVRDGEVELGIVEAIVDDPALAQWPL
GEDRLTIIQADPCLPRTVDRAWLRAARWVMREPGSGTRSTLEQHLRHLGIEPEDLTISLVLPSNESVRTAIEAGAGIGAL
SSLVVAPALQAGTVHAVPQALGARSFYGLRHKERYRSPAAEALLKLIGQEPPGTPNPVG
>Mature_298_residues
TLEQLRLFIAVAEREHVTAASRALNVTQSAVSAAIATLEERHGVKLFHRVGRGIRLTDAGRLFLPEARAVLARAVAAENV
LDDFSGLKRGTLRVVASQTIAAYWLPPILVSFRKQYPGITIDLAISNTEDAAARVRDGEVELGIVEAIVDDPALAQWPLG
EDRLTIIQADPCLPRTVDRAWLRAARWVMREPGSGTRSTLEQHLRHLGIEPEDLTISLVLPSNESVRTAIEAGAGIGALS
SLVVAPALQAGTVHAVPQALGARSFYGLRHKERYRSPAAEALLKLIGQEPPGTPNPVG

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788481, Length=300, Percent_Identity=34, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI157672245, Length=291, Percent_Identity=30.5841924398625, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI87082132, Length=298, Percent_Identity=27.5167785234899, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI145693105, Length=290, Percent_Identity=26.551724137931, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1788748, Length=302, Percent_Identity=28.1456953642384, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1790399, Length=261, Percent_Identity=26.4367816091954, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1786401, Length=266, Percent_Identity=26.3157894736842, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1790208, Length=251, Percent_Identity=27.4900398406374, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI1787601, Length=145, Percent_Identity=28.9655172413793, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1787128, Length=129, Percent_Identity=31.0077519379845, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788296, Length=162, Percent_Identity=29.0123456790123, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787530, Length=253, Percent_Identity=24.5059288537549, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1789639, Length=122, Percent_Identity=33.6065573770492, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1789173, Length=143, Percent_Identity=34.965034965035, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI87081904, Length=153, Percent_Identity=32.6797385620915, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1790262, Length=294, Percent_Identity=25.5102040816327, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1787879, Length=171, Percent_Identity=28.6549707602339, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI145693193, Length=260, Percent_Identity=26.5384615384615, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1787806, Length=179, Percent_Identity=27.3743016759777, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32230; Mature: 32099

Theoretical pI: Translated: 7.81; Mature: 7.81

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLEQLRLFIAVAEREHVTAASRALNVTQSAVSAAIATLEERHGVKLFHRVGRGIRLTDA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCEEEECC
GRLFLPEARAVLARAVAAENVLDDFSGLKRGTLRVVASQTIAAYWLPPILVSFRKQYPGI
CCEECHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
TIDLAISNTEDAAARVRDGEVELGIVEAIVDDPALAQWPLGEDRLTIIQADPCLPRTVDR
EEEEEECCCHHHHHHHCCCCEEHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCHHHHH
AWLRAARWVMREPGSGTRSTLEQHLRHLGIEPEDLTISLVLPSNESVRTAIEAGAGIGAL
HHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHCCCHHHH
SSLVVAPALQAGTVHAVPQALGARSFYGLRHKERYRSPAAEALLKLIGQEPPGTPNPVG
HHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TLEQLRLFIAVAEREHVTAASRALNVTQSAVSAAIATLEERHGVKLFHRVGRGIRLTDA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCEEEECC
GRLFLPEARAVLARAVAAENVLDDFSGLKRGTLRVVASQTIAAYWLPPILVSFRKQYPGI
CCEECHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
TIDLAISNTEDAAARVRDGEVELGIVEAIVDDPALAQWPLGEDRLTIIQADPCLPRTVDR
EEEEEECCCHHHHHHHCCCCEEHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCHHHHH
AWLRAARWVMREPGSGTRSTLEQHLRHLGIEPEDLTISLVLPSNESVRTAIEAGAGIGAL
HHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHCCCHHHH
SSLVVAPALQAGTVHAVPQALGARSFYGLRHKERYRSPAAEALLKLIGQEPPGTPNPVG
HHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]