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The map label for this gene is pckA [H]

Identifier: 162146435

GI number: 162146435

Start: 618218

End: 619837

Strand: Direct

Name: pckA [H]

Synonym: GDI_0610

Alternate gene names: 162146435

Gene position: 618218-619837 (Clockwise)

Preceding gene: 162146431

Following gene: 162146436

Centisome position: 15.67

GC content: 69.75

Gene sequence:

>1620_bases
ATGAAGAACAAGAACGGTTTCCGGCCTGCGGGTTCCGACAGCGTCCTGGCGGGAACCGGCCTGCGTCATGCCGGCCCGGT
CCATGCCAACCTCCATGCCCCCGAACTGACCGAATGCGCGATCCGCCGGGGCGAGGGCAGCCTGTCCGCCGGCGGGGCGC
TGATGGTACGGACGGGGGTGCATACCGGCCGGTCGGCGCAGGACAAATTCATCGTCGACGAACCGGCGACGCGCGCCGGC
ATCTGGTGGGGCGACATCAACATGCCCCTGCCGGTCGCCGCGTTCCAGCGCGTCTGCGACCATGCGCGCGGATACCTGGA
AGGGCAGGAACTGTTCACCCAGGACCTGTATGCCGGCGCCGACCCGGCCCATCGCATCCGCATCCGGCTGGTCACGACCT
CGGCATGGCACGCCCTGTTCGCGCGCAATATGTTCATCCGCCCCCCGGCGGCCGACCTGCCGGGATTCCAGCCGGATTAC
GTGATCCTGCACGCGCCCGAACTGGACCTGGACCCGGCGGTGCACGGGGTGCGCAGCAGCACGGGCGTCATCCTGTCGCT
GGAACAGCGCCTGATCGTGATCGCCGGGACCGGGTACGCGGGCGAGATCAAGAAATCGATCTTCACGGCCATGAACTGGC
TGCTGCCGGACCAGGACGTGATGCCGATGCATTGTTCGGCCAATATCGGGCCGGATGGTGACGTGGCGCTGTTCTTCGGC
CTGTCGGGCACCGGCAAGACCACGCTGTCGTCCGATGCGTCGCGGCATCTGATCGGCGATGACGAACATGGCTGGTCGGG
CCGGGGCGTCTTCAATATCGAAGGGGGCTGCTACGCCAAGGTGATCGGGTTGCGGCAGGCGGCCGAACCGGAAATCTGGG
ACGCATCGCGGCACTTCGGCGCGGTGCTGGAAAACGTGGTGGCCGACGCGCACGGCGTGCCGGATTTCGCGGACGGCAGC
CTGACGGAAAACACGCGGGCCTGCTACCCGATCGATTTCGTCGCCAATGCCAGCGCGGACGGGCAGGGCGGGCTGCCGCG
CCATGTGGTGATGCTGACGGCCGACGCGTTCGGCGTGCTGCCGCCGATCGCGCGGCTGACGCCGGCGCAGGCGGTCTATC
ATTTCCTGTCGGGCTACACAGCACGGATCGCCGGCACCGAAAAGGGGCTGGGGAAGGAGCCGCAGGCGACGTTCAGCGCC
TGCTTCGGCGCGCCGTTCCTACCCCGCCCGCCGGAACTGTACGGCGACCGGCTGGCGGCCTGCCTGGCGCGCGTGCCGGT
CACGTGCTGGCTGGTCAATACCGGCTGGACCGGCGGCCCGTACGGCGTGGGGGACCGGATCTCGCTCGCGCATACACGGG
CCCTGGTCAACGCGGCGCTGGACGGGCGGCTGGACGACGCCCCCTTCCAGGTCGAGCCGCATTTCGGCCTGTCCATTCCC
GCCCGGGTGCCCGGCATCCCCGCCGCGCTGCTGGACCCGGCGGCGGCCTGGGCCGATCCGGACGCCTACCGCCATGCCGC
GTCGGACCTCGCGGCGCGGTTCGAACGGAATTTCGCGGCGTTCCACGCGCGCTGCACGCCCGACATCCAGGCCGCGGCGA
TCCGTCCCGTCCGGCCATAG

Upstream 100 bases:

>100_bases
AAACCGTTAGAATACATAAGGAATTTGCGACAATAGCCGTGGGGTGACGGACAGGGCGCTGCTATGCTGCGGGTCACGCC
ATATTCAGGAAGCGGAAAGA

Downstream 100 bases:

>100_bases
CAAAAAACAACCAGCACCCGGCGGCGGCGTTCGGTGTCAGTGCAGGCTGCCCCGGTGCCGCCCCCGGCAGTCCTCTGAGT
ACCGCAGGAGGCTGCATGAC

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase [H]

Number of amino acids: Translated: 539; Mature: 539

Protein sequence:

>539_residues
MKNKNGFRPAGSDSVLAGTGLRHAGPVHANLHAPELTECAIRRGEGSLSAGGALMVRTGVHTGRSAQDKFIVDEPATRAG
IWWGDINMPLPVAAFQRVCDHARGYLEGQELFTQDLYAGADPAHRIRIRLVTTSAWHALFARNMFIRPPAADLPGFQPDY
VILHAPELDLDPAVHGVRSSTGVILSLEQRLIVIAGTGYAGEIKKSIFTAMNWLLPDQDVMPMHCSANIGPDGDVALFFG
LSGTGKTTLSSDASRHLIGDDEHGWSGRGVFNIEGGCYAKVIGLRQAAEPEIWDASRHFGAVLENVVADAHGVPDFADGS
LTENTRACYPIDFVANASADGQGGLPRHVVMLTADAFGVLPPIARLTPAQAVYHFLSGYTARIAGTEKGLGKEPQATFSA
CFGAPFLPRPPELYGDRLAACLARVPVTCWLVNTGWTGGPYGVGDRISLAHTRALVNAALDGRLDDAPFQVEPHFGLSIP
ARVPGIPAALLDPAAAWADPDAYRHAASDLAARFERNFAAFHARCTPDIQAAAIRPVRP

Sequences:

>Translated_539_residues
MKNKNGFRPAGSDSVLAGTGLRHAGPVHANLHAPELTECAIRRGEGSLSAGGALMVRTGVHTGRSAQDKFIVDEPATRAG
IWWGDINMPLPVAAFQRVCDHARGYLEGQELFTQDLYAGADPAHRIRIRLVTTSAWHALFARNMFIRPPAADLPGFQPDY
VILHAPELDLDPAVHGVRSSTGVILSLEQRLIVIAGTGYAGEIKKSIFTAMNWLLPDQDVMPMHCSANIGPDGDVALFFG
LSGTGKTTLSSDASRHLIGDDEHGWSGRGVFNIEGGCYAKVIGLRQAAEPEIWDASRHFGAVLENVVADAHGVPDFADGS
LTENTRACYPIDFVANASADGQGGLPRHVVMLTADAFGVLPPIARLTPAQAVYHFLSGYTARIAGTEKGLGKEPQATFSA
CFGAPFLPRPPELYGDRLAACLARVPVTCWLVNTGWTGGPYGVGDRISLAHTRALVNAALDGRLDDAPFQVEPHFGLSIP
ARVPGIPAALLDPAAAWADPDAYRHAASDLAARFERNFAAFHARCTPDIQAAAIRPVRP
>Mature_539_residues
MKNKNGFRPAGSDSVLAGTGLRHAGPVHANLHAPELTECAIRRGEGSLSAGGALMVRTGVHTGRSAQDKFIVDEPATRAG
IWWGDINMPLPVAAFQRVCDHARGYLEGQELFTQDLYAGADPAHRIRIRLVTTSAWHALFARNMFIRPPAADLPGFQPDY
VILHAPELDLDPAVHGVRSSTGVILSLEQRLIVIAGTGYAGEIKKSIFTAMNWLLPDQDVMPMHCSANIGPDGDVALFFG
LSGTGKTTLSSDASRHLIGDDEHGWSGRGVFNIEGGCYAKVIGLRQAAEPEIWDASRHFGAVLENVVADAHGVPDFADGS
LTENTRACYPIDFVANASADGQGGLPRHVVMLTADAFGVLPPIARLTPAQAVYHFLSGYTARIAGTEKGLGKEPQATFSA
CFGAPFLPRPPELYGDRLAACLARVPVTCWLVNTGWTGGPYGVGDRISLAHTRALVNAALDGRLDDAPFQVEPHFGLSIP
ARVPGIPAALLDPAAAWADPDAYRHAASDLAARFERNFAAFHARCTPDIQAAAIRPVRP

Specific function: Rate-limiting gluconeogenic enzyme. [C]

COG id: COG1866

COG function: function code C; Phosphoenolpyruvate carboxykinase (ATP)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [ATP] family [H]

Homologues:

Organism=Escherichia coli, GI1789807, Length=506, Percent_Identity=45.8498023715415, Blast_Score=439, Evalue=1e-124,
Organism=Saccharomyces cerevisiae, GI6322950, Length=517, Percent_Identity=44.8742746615087, Blast_Score=444, Evalue=1e-125,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001272
- InterPro:   IPR013035
- InterPro:   IPR008210
- InterPro:   IPR015994 [H]

Pfam domain/function: PF01293 PEPCK_ATP [H]

EC number: =4.1.1.49 [H]

Molecular weight: Translated: 57465; Mature: 57465

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNKNGFRPAGSDSVLAGTGLRHAGPVHANLHAPELTECAIRRGEGSLSAGGALMVRTGV
CCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCEEEEEECC
HTGRSAQDKFIVDEPATRAGIWWGDINMPLPVAAFQRVCDHARGYLEGQELFTQDLYAGA
CCCCCCCCCEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCC
DPAHRIRIRLVTTSAWHALFARNMFIRPPAADLPGFQPDYVILHAPELDLDPAVHGVRSS
CCCCEEEEEEEECHHHHHHHHCCEEECCCCCCCCCCCCCEEEEECCCCCCCHHHHCCCCC
TGVILSLEQRLIVIAGTGYAGEIKKSIFTAMNWLLPDQDVMPMHCSANIGPDGDVALFFG
CCEEEEEECEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCEEEEEE
LSGTGKTTLSSDASRHLIGDDEHGWSGRGVFNIEGGCYAKVIGLRQAAEPEIWDASRHFG
ECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEEEEEHHHCCCCCHHCCHHHHH
AVLENVVADAHGVPDFADGSLTENTRACYPIDFVANASADGQGGLPRHVVMLTADAFGVL
HHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCEEEEEEEECHHHCC
PPIARLTPAQAVYHFLSGYTARIAGTEKGLGKEPQATFSACFGAPFLPRPPELYGDRLAA
CCHHHCCHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHH
CLARVPVTCWLVNTGWTGGPYGVGDRISLAHTRALVNAALDGRLDDAPFQVEPHFGLSIP
HHHHCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCC
ARVPGIPAALLDPAAAWADPDAYRHAASDLAARFERNFAAFHARCTPDIQAAAIRPVRP
CCCCCCCHHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHCCCCCCC
>Mature Secondary Structure
MKNKNGFRPAGSDSVLAGTGLRHAGPVHANLHAPELTECAIRRGEGSLSAGGALMVRTGV
CCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCEEEEEECC
HTGRSAQDKFIVDEPATRAGIWWGDINMPLPVAAFQRVCDHARGYLEGQELFTQDLYAGA
CCCCCCCCCEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCC
DPAHRIRIRLVTTSAWHALFARNMFIRPPAADLPGFQPDYVILHAPELDLDPAVHGVRSS
CCCCEEEEEEEECHHHHHHHHCCEEECCCCCCCCCCCCCEEEEECCCCCCCHHHHCCCCC
TGVILSLEQRLIVIAGTGYAGEIKKSIFTAMNWLLPDQDVMPMHCSANIGPDGDVALFFG
CCEEEEEECEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCEEEEEE
LSGTGKTTLSSDASRHLIGDDEHGWSGRGVFNIEGGCYAKVIGLRQAAEPEIWDASRHFG
ECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEEEEEHHHCCCCCHHCCHHHHH
AVLENVVADAHGVPDFADGSLTENTRACYPIDFVANASADGQGGLPRHVVMLTADAFGVL
HHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCEEEEEEEECHHHCC
PPIARLTPAQAVYHFLSGYTARIAGTEKGLGKEPQATFSACFGAPFLPRPPELYGDRLAA
CCHHHCCHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHH
CLARVPVTCWLVNTGWTGGPYGVGDRISLAHTRALVNAALDGRLDDAPFQVEPHFGLSIP
HHHHCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCC
ARVPGIPAALLDPAAAWADPDAYRHAASDLAARFERNFAAFHARCTPDIQAAAIRPVRP
CCCCCCCHHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA