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The map label for this gene is entA [C]

Identifier: 162146380

GI number: 162146380

Start: 560610

End: 561581

Strand: Reverse

Name: entA [C]

Synonym: GDI_0555

Alternate gene names: 162146380

Gene position: 561581-560610 (Counterclockwise)

Preceding gene: 162146390

Following gene: 162146378

Centisome position: 14.24

GC content: 69.44

Gene sequence:

>972_bases
ATGGCTGAATCCTTCAATGCCACGTCGACCACCGACGATGTGCTGGCGGGTGTCGATCTGCGCGGCAAGCGCGTGCTTGT
GACCGGCGTGTCCGCCGGACTGGGTGTGGAAACCGCCCGCGCCCTTGCCGCCCATGGCGCGCACGTCGTCGGCGCGGCGC
GCGACCTGGCCAAGGCCGAGCGCGCGACGGCGCATGTCCGCGCCGCCGCCGCCCATGGCGGCGGGCTGGACCTGATCGAA
CTGGACCTGGCCGCGCTGGCCAGCGTGCGCGCCTGCGCCAACGCCCTGCACGCGACCGGCAAGCCCTTCGACCTGGTCGT
CGCCAATGCGGGCGTGATGGCCTCCCCCTTCGGCCACACGGCGGATGGGTTCGAAACCCAGTTCGGGACCAACCATCTGG
GCCATTTCGTGCTGGTCAACCGGATTGCCGCGCTGATGCGGCCGGGATCGCGGCTGGTCAGCCTGGCGTCCTCGGGCCAT
CGCTTCGCGGATGTCGACCTCGACGATCCGAATTTCGACCATACGCCGTACGATCCCTTCGTCGCCTACGGCCGGTCCAA
GACCGCGAATATCCTGTTCGCGGTGGAATTCGACCGCCGGCACAAGGATCGCGGCGTGCGGGCCACGGCGGTGCATCCCG
GCGGAATCCGGACGGAACTCGCCCGCTACATGGACCCCGCGCATCTTCAGGCGATGATCGACGGCATCAACGCCGACCTG
GCCGCCGAGGGCAGGCCCCCCTTCGCATGGAAGACGATCCCGCAAGGGGCCGCGACCTCGGTCTGGGCCGGAATCGTCGC
CCCGGCCGCAGAGGTCGGCGGGCGGTATTGCGAGAACTGCCACGTGAGCGAGGTCGTGGCGGACCGCAAGATCAGCGTCA
TGAGCGAGGGCGTGCGCCCCTACGCCCTGGACCCCGAGCATGCCAGGGCGCTGTGGGCGAAGAGCGAGGACATGGTCGGC
GAAACGTTCTGA

Upstream 100 bases:

>100_bases
GGAGGGACCCTCCGTATTTAAACGGAGGCATTCTCCTTTTATATGCCGCACCGCCTTTCCCTCGGCAATGCAGGCCGGGC
GTTTCACAAAGGACAGAATC

Downstream 100 bases:

>100_bases
CCGCTATCCGTTGGCGGGGATCGTCACGATCGTGCTGGGGCCCTTGCTGGTCTTCGACAGCTCCAGCAGAATCTGCAGCA
TGGTGAAGGCCAGCTTGCTG

Product: oxidoreductase

Products: 2,3-Dihydroxybenzoate; H+; Nicotinamide adenine dinucleotide - reduced [C]

Alternate protein names: NA

Number of amino acids: Translated: 323; Mature: 322

Protein sequence:

>323_residues
MAESFNATSTTDDVLAGVDLRGKRVLVTGVSAGLGVETARALAAHGAHVVGAARDLAKAERATAHVRAAAAHGGGLDLIE
LDLAALASVRACANALHATGKPFDLVVANAGVMASPFGHTADGFETQFGTNHLGHFVLVNRIAALMRPGSRLVSLASSGH
RFADVDLDDPNFDHTPYDPFVAYGRSKTANILFAVEFDRRHKDRGVRATAVHPGGIRTELARYMDPAHLQAMIDGINADL
AAEGRPPFAWKTIPQGAATSVWAGIVAPAAEVGGRYCENCHVSEVVADRKISVMSEGVRPYALDPEHARALWAKSEDMVG
ETF

Sequences:

>Translated_323_residues
MAESFNATSTTDDVLAGVDLRGKRVLVTGVSAGLGVETARALAAHGAHVVGAARDLAKAERATAHVRAAAAHGGGLDLIE
LDLAALASVRACANALHATGKPFDLVVANAGVMASPFGHTADGFETQFGTNHLGHFVLVNRIAALMRPGSRLVSLASSGH
RFADVDLDDPNFDHTPYDPFVAYGRSKTANILFAVEFDRRHKDRGVRATAVHPGGIRTELARYMDPAHLQAMIDGINADL
AAEGRPPFAWKTIPQGAATSVWAGIVAPAAEVGGRYCENCHVSEVVADRKISVMSEGVRPYALDPEHARALWAKSEDMVG
ETF
>Mature_322_residues
AESFNATSTTDDVLAGVDLRGKRVLVTGVSAGLGVETARALAAHGAHVVGAARDLAKAERATAHVRAAAAHGGGLDLIEL
DLAALASVRACANALHATGKPFDLVVANAGVMASPFGHTADGFETQFGTNHLGHFVLVNRIAALMRPGSRLVSLASSGHR
FADVDLDDPNFDHTPYDPFVAYGRSKTANILFAVEFDRRHKDRGVRATAVHPGGIRTELARYMDPAHLQAMIDGINADLA
AEGRPPFAWKTIPQGAATSVWAGIVAPAAEVGGRYCENCHVSEVVADRKISVMSEGVRPYALDPEHARALWAKSEDMVGE
TF

Specific function: Siderophore biosynthesis; enterobactin biosynthesis. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI7706523, Length=328, Percent_Identity=35.6707317073171, Blast_Score=178, Evalue=5e-45,
Organism=Homo sapiens, GI10190746, Length=321, Percent_Identity=36.1370716510903, Blast_Score=165, Evalue=5e-41,
Organism=Homo sapiens, GI225579078, Length=305, Percent_Identity=34.4262295081967, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI186928839, Length=305, Percent_Identity=33.7704918032787, Blast_Score=146, Evalue=2e-35,
Organism=Homo sapiens, GI166795268, Length=308, Percent_Identity=34.0909090909091, Blast_Score=145, Evalue=6e-35,
Organism=Homo sapiens, GI19923931, Length=246, Percent_Identity=34.5528455284553, Blast_Score=122, Evalue=3e-28,
Organism=Homo sapiens, GI193804850, Length=306, Percent_Identity=29.4117647058824, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI146231950, Length=310, Percent_Identity=31.9354838709677, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI193207620, Length=330, Percent_Identity=30.9090909090909, Blast_Score=155, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17532805, Length=327, Percent_Identity=32.7217125382263, Blast_Score=150, Evalue=9e-37,
Organism=Caenorhabditis elegans, GI17532791, Length=276, Percent_Identity=31.8840579710145, Blast_Score=138, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI71992402, Length=317, Percent_Identity=30.2839116719243, Blast_Score=124, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI17535059, Length=324, Percent_Identity=29.9382716049383, Blast_Score=120, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI32565532, Length=225, Percent_Identity=31.1111111111111, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17505416, Length=316, Percent_Identity=28.7974683544304, Blast_Score=97, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI17558006, Length=313, Percent_Identity=26.517571884984, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI72001364, Length=217, Percent_Identity=30.4147465437788, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324820, Length=227, Percent_Identity=30.8370044052863, Blast_Score=85, Evalue=2e-17,
Organism=Drosophila melanogaster, GI20129347, Length=327, Percent_Identity=30.8868501529052, Blast_Score=159, Evalue=2e-39,
Organism=Drosophila melanogaster, GI28573296, Length=307, Percent_Identity=32.5732899022801, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI22024069, Length=307, Percent_Identity=32.8990228013029, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24586328, Length=335, Percent_Identity=31.6417910447761, Blast_Score=140, Evalue=8e-34,
Organism=Drosophila melanogaster, GI45554248, Length=299, Percent_Identity=32.7759197324415, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24640117, Length=299, Percent_Identity=32.7759197324415, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI19921754, Length=305, Percent_Identity=31.1475409836066, Blast_Score=131, Evalue=5e-31,
Organism=Drosophila melanogaster, GI161076371, Length=303, Percent_Identity=33.6633663366337, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI45553409, Length=301, Percent_Identity=28.9036544850498, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24647948, Length=301, Percent_Identity=28.9036544850498, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24647946, Length=320, Percent_Identity=28.125, Blast_Score=108, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24656154, Length=266, Percent_Identity=30.4511278195489, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI19922628, Length=266, Percent_Identity=30.4511278195489, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24647931, Length=289, Percent_Identity=25.6055363321799, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.3.1.28 [C]

Molecular weight: Translated: 34058; Mature: 33927

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAESFNATSTTDDVLAGVDLRGKRVLVTGVSAGLGVETARALAAHGAHVVGAARDLAKAE
CCCCCCCCCCCHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHH
RATAHVRAAAAHGGGLDLIELDLAALASVRACANALHATGKPFDLVVANAGVMASPFGHT
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEECCCCCC
ADGFETQFGTNHLGHFVLVNRIAALMRPGSRLVSLASSGHRFADVDLDDPNFDHTPYDPF
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCEEEECCCCCCCCCCCCCCHH
VAYGRSKTANILFAVEFDRRHKDRGVRATAVHPGGIRTELARYMDPAHLQAMIDGINADL
HHCCCCCCCEEEEEEEECCHHHCCCCEEEEECCCCHHHHHHHHCCHHHHHHHHHCCCCCE
AAEGRPPFAWKTIPQGAATSVWAGIVAPAAEVGGRYCENCHVSEVVADRKISVMSEGVRP
ECCCCCCCCEECCCCCCHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHCCCCC
YALDPEHARALWAKSEDMVGETF
CCCCHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
AESFNATSTTDDVLAGVDLRGKRVLVTGVSAGLGVETARALAAHGAHVVGAARDLAKAE
CCCCCCCCCCHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHH
RATAHVRAAAAHGGGLDLIELDLAALASVRACANALHATGKPFDLVVANAGVMASPFGHT
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEECCCCCC
ADGFETQFGTNHLGHFVLVNRIAALMRPGSRLVSLASSGHRFADVDLDDPNFDHTPYDPF
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCEEEECCCCCCCCCCCCCCHH
VAYGRSKTANILFAVEFDRRHKDRGVRATAVHPGGIRTELARYMDPAHLQAMIDGINADL
HHCCCCCCCEEEEEEEECCHHHCCCCEEEEECCCCHHHHHHHHCCHHHHHHHHHCCCCCE
AAEGRPPFAWKTIPQGAATSVWAGIVAPAAEVGGRYCENCHVSEVVADRKISVMSEGVRP
ECCCCCCCCEECCCCCCHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHCCCCC
YALDPEHARALWAKSEDMVGETF
CCCCHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 5550 [C]

Specific activity: 66

Km value (mM): 0.26 {methyl-2,3-dehydro-3-hydroxybenzoate}} 0.2 {3-oxocyclohexane-1-carboxylic} 0.3 {2,3-dihydro-2,3-dihydroxybenzoate}} [C]

Substrates: 2,3-Dihydro-2,3-dihydroxybenzoate; Nicotinamide adenine dinucleotide [C]

Specific reaction: 2,3-Dihydro-2,3-dihydroxybenzoate + Nicotinamide adenine dinucleotide <==> 2,3-Dihydroxybenzoate + H+ + Nicotinamide adenine dinucleotide - reduced [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8383668 [H]