Click here to switch to the map view.

The map label for this gene is bcr [H]

Identifier: 162146357

GI number: 162146357

Start: 526915

End: 528090

Strand: Direct

Name: bcr [H]

Synonym: GDI_0532

Alternate gene names: 162146357

Gene position: 526915-528090 (Clockwise)

Preceding gene: 162146356

Following gene: 162146363

Centisome position: 13.36

GC content: 69.81

Gene sequence:

>1176_bases
ATGATCAAGACTTCGCCTTTTGCGATGACGGCCGTCCTTGCCATCCTGACGGCGGTCGGCCCGATTTCGACCGATATGTA
TCTTCCCGCCTTCGGGGCCATGACTCACGCCCTGGGCGGCGCGCATGGCGATGCCGAAATGACGCTGGCGGCCTGGTTCC
TCGGCCTGTCGGTGGGGCAGGTCACCCATGGCGCGATCGCCGACCATTACGGGCGGCGCGGGCCGCTGCTGGGCGGCACC
CTGCTTTATACCATCGCCTCGGTCGGCTGCGCGGTGGCGGGTTCGATGTGGGCATTTTCGCTCTGGCGGTTCGTCGCGGC
GTTCGGGGCGGCCGCCAGCCTCGTGATCCCGCGCGCCGTCATCCGCGATGTGGTGGGGGGCGACGGCGACGCGGCGCGGA
TGCTGGGCCGGATGGTGCTGGTGATGAGCATCGTGCCGATGCTGGCACCGACCCTGGGCGGCGTGATTGCCGAGGACTGG
CAGTGGCGCGGTATCTTCTGGATAGCGGCGGCATACGGCGCCTGCTGCGTGGCCATGGTCTACTGGCTGCTGCCCGATAC
GCTGACCACCGAACTGCATATGCGCTTTCGGCTGGAACAAGCGGTCACGGGCTATGTCCTGATCCTGCATGACCGCGTGT
TCCGCCTGCACGCGCTGGAGGGCGGGTGCGCCACCTTCACCCTGTTCGCATTCCTGGGCGGTGCGCCACGGGTTTTCGTG
GCGGGGTACGGGCTTTCGCCCGTGCAGTTCGGCGGCATCTTCATCCTGAACGCGGCGGGCTATGCCGTCGGCACCAACAT
CAACGGGCGGCTGGTGCCGCGGCTGGGCGCCCCCCGCGTGCTCAGCGGCGCGGCCGTGGCGCTGGCGGTGGCGTGCGGCG
GCATGTTCGCGGCGGCCCTGCTGCATGTCGGGGGCGGGATCCTGCAGGTCGGCGGGATCACCCTGTGCATGGCCATCCTG
GGCTTCCTGCTGCCGGGCGCGGCCCTGGGGTCGGTCCTGCCGAACGGGGCCAGGGCGGCGACGGCCTCGGCGCTGTATGG
AACCGTGGTGTTCTTCATCGGCTCGTTCAGCACGATGCTGGTGGGCTGGCTGGGCACGCGCGGGCCCGCGTCGATGACGG
GGCTGATGCTGCTGGGCGCCCTGATGGCCCTGGCCTGCGACTGGCGGCGGCGATAG

Upstream 100 bases:

>100_bases
ATGAAAATCCCACAGGGATTTATGGGCGGATAGCCCCTGTGTTATCCGCGATGATCGTCCTACCATGGCCCGGCCCGCAG
ATGGCCGGGGCCGGGGAATG

Downstream 100 bases:

>100_bases
GCGAGCGGCGATAGGGGACCCGGTGCCCTATCCCGCCATGCCGGCCGCCGCCATCAGCAGGTGCATCAGCATGGCGAACA
CGCCCAGGGCCAGCACGCTG

Product: MFS transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Sulfonamide resistance protein [H]

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MIKTSPFAMTAVLAILTAVGPISTDMYLPAFGAMTHALGGAHGDAEMTLAAWFLGLSVGQVTHGAIADHYGRRGPLLGGT
LLYTIASVGCAVAGSMWAFSLWRFVAAFGAAASLVIPRAVIRDVVGGDGDAARMLGRMVLVMSIVPMLAPTLGGVIAEDW
QWRGIFWIAAAYGACCVAMVYWLLPDTLTTELHMRFRLEQAVTGYVLILHDRVFRLHALEGGCATFTLFAFLGGAPRVFV
AGYGLSPVQFGGIFILNAAGYAVGTNINGRLVPRLGAPRVLSGAAVALAVACGGMFAAALLHVGGGILQVGGITLCMAIL
GFLLPGAALGSVLPNGARAATASALYGTVVFFIGSFSTMLVGWLGTRGPASMTGLMLLGALMALACDWRRR

Sequences:

>Translated_391_residues
MIKTSPFAMTAVLAILTAVGPISTDMYLPAFGAMTHALGGAHGDAEMTLAAWFLGLSVGQVTHGAIADHYGRRGPLLGGT
LLYTIASVGCAVAGSMWAFSLWRFVAAFGAAASLVIPRAVIRDVVGGDGDAARMLGRMVLVMSIVPMLAPTLGGVIAEDW
QWRGIFWIAAAYGACCVAMVYWLLPDTLTTELHMRFRLEQAVTGYVLILHDRVFRLHALEGGCATFTLFAFLGGAPRVFV
AGYGLSPVQFGGIFILNAAGYAVGTNINGRLVPRLGAPRVLSGAAVALAVACGGMFAAALLHVGGGILQVGGITLCMAIL
GFLLPGAALGSVLPNGARAATASALYGTVVFFIGSFSTMLVGWLGTRGPASMTGLMLLGALMALACDWRRR
>Mature_391_residues
MIKTSPFAMTAVLAILTAVGPISTDMYLPAFGAMTHALGGAHGDAEMTLAAWFLGLSVGQVTHGAIADHYGRRGPLLGGT
LLYTIASVGCAVAGSMWAFSLWRFVAAFGAAASLVIPRAVIRDVVGGDGDAARMLGRMVLVMSIVPMLAPTLGGVIAEDW
QWRGIFWIAAAYGACCVAMVYWLLPDTLTTELHMRFRLEQAVTGYVLILHDRVFRLHALEGGCATFTLFAFLGGAPRVFV
AGYGLSPVQFGGIFILNAAGYAVGTNINGRLVPRLGAPRVLSGAAVALAVACGGMFAAALLHVGGGILQVGGITLCMAIL
GFLLPGAALGSVLPNGARAATASALYGTVVFFIGSFSTMLVGWLGTRGPASMTGLMLLGALMALACDWRRR

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=357, Percent_Identity=32.2128851540616, Blast_Score=149, Evalue=4e-37,
Organism=Escherichia coli, GI48994889, Length=252, Percent_Identity=26.5873015873016, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1790146, Length=330, Percent_Identity=24.5454545454545, Blast_Score=81, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6324264, Length=186, Percent_Identity=26.8817204301075, Blast_Score=66, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 40514; Mature: 40514

Theoretical pI: Translated: 9.06; Mature: 9.06

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKTSPFAMTAVLAILTAVGPISTDMYLPAFGAMTHALGGAHGDAEMTLAAWFLGLSVGQ
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
VTHGAIADHYGRRGPLLGGTLLYTIASVGCAVAGSMWAFSLWRFVAAFGAAASLVIPRAV
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IRDVVGGDGDAARMLGRMVLVMSIVPMLAPTLGGVIAEDWQWRGIFWIAAAYGACCVAMV
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHHHHHHH
YWLLPDTLTTELHMRFRLEQAVTGYVLILHDRVFRLHALEGGCATFTLFAFLGGAPRVFV
HHHCCCHHHHHHHHHHHHHHHHCCEEEEEEHHHHHEEECCCCHHHHHHHHHHCCCCEEEE
AGYGLSPVQFGGIFILNAAGYAVGTNINGRLVPRLGAPRVLSGAAVALAVACGGMFAAAL
EECCCCCEEECCEEEEECCCEEEECCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
LHVGGGILQVGGITLCMAILGFLLPGAALGSVLPNGARAATASALYGTVVFFIGSFSTML
HHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
VGWLGTRGPASMTGLMLLGALMALACDWRRR
HHHHCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIKTSPFAMTAVLAILTAVGPISTDMYLPAFGAMTHALGGAHGDAEMTLAAWFLGLSVGQ
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
VTHGAIADHYGRRGPLLGGTLLYTIASVGCAVAGSMWAFSLWRFVAAFGAAASLVIPRAV
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IRDVVGGDGDAARMLGRMVLVMSIVPMLAPTLGGVIAEDWQWRGIFWIAAAYGACCVAMV
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHHHHHHH
YWLLPDTLTTELHMRFRLEQAVTGYVLILHDRVFRLHALEGGCATFTLFAFLGGAPRVFV
HHHCCCHHHHHHHHHHHHHHHHCCEEEEEEHHHHHEEECCCCHHHHHHHHHHCCCCEEEE
AGYGLSPVQFGGIFILNAAGYAVGTNINGRLVPRLGAPRVLSGAAVALAVACGGMFAAAL
EECCCCCEEECCEEEEECCCEEEECCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
LHVGGGILQVGGITLCMAILGFLLPGAALGSVLPNGARAATASALYGTVVFFIGSFSTML
HHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
VGWLGTRGPASMTGLMLLGALMALACDWRRR
HHHHCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8486276; 9278503; 2694948 [H]