Click here to switch to the map view.

The map label for this gene is gspO [H]

Identifier: 162146305

GI number: 162146305

Start: 478157

End: 478942

Strand: Direct

Name: gspO [H]

Synonym: GDI_0480

Alternate gene names: 162146305

Gene position: 478157-478942 (Clockwise)

Preceding gene: 162146304

Following gene: 162146306

Centisome position: 12.12

GC content: 73.28

Gene sequence:

>786_bases
ATGCTGCATCTACCCGTCCTTCCCCTGCTCCTGTCCCCCTTCATCGGCTCTTTCCTGGGGGTGCTGGCCGCGCGCCTGCC
CCGCAGGCTGCCGGTGGCGCTGGCCCGGTCGGCCTGCCCGCGCTGCGGGGCCGTGCTGCGCCCGCGCGAACTGGTGCCCG
TCGTCAGCTATCTGGTGCAGAAGGGACGGTGCCGGTCCTGCGGCGGCGCCATTTCGGCCGGGCATCCCGCCATGGAAGTG
GCGGCGCTGCTGGTCGCGGGCCTGGTCCTGCTGGCCGCCGGTCCGTTGCGCGCCGGCACGCCGGCCCCGTTGCTGGTGGT
CTGGAGCGGCTGCCTGTTCGGGTGGTGGCTGCTGGTGCTGGCCGCGATCGACCTGAAAAGCTTCCGCCTGCCCGACACGC
TGACAGTGCCGTTGATCGCGGCCGGGCTGGGGCTGGCGGCCCTGGGGGGTGCCGCGCCGGTGCTGTGGCACGCCCTGGCG
GCGGCGGCGGGGTACGGCGTCTTTCGCGGGATTTCGCTTCTGTATCGCCGGGCGCGCGGCCATGATGGGCTCGGCGGGGG
CGATGCCAAGCTGCTGGCCGCCGGGGGGGCGTGGCTCGGTCCCGAATCCCTGCCGCATGTCGTCTTCCTGGGGGCCGTGT
TGACGCTGTGCGCGGTCGCGATCGCCCGGCGTGGTGATGTGAATCGCACGCTGATGGTCCCGTTCGGGCCGGGACTGGCG
CTGGCGATGTGGGGAATCTGGCTGTGGCAGGCCAGCCTGCTCCGGCCGGACTGGCTGCCCGCATGA

Upstream 100 bases:

>100_bases
CCGAACGCGCGGGGCATGATTACGATCTCTGCTCGGCCGGTTCCGGCGGCAACGCCGCCAGCACGGCGACACAGATCTGC
AATCCCTGATCGTGGCGTCC

Downstream 100 bases:

>100_bases
CCCTCAGGAATGGCGGGGCATCATGATGGACGCGGGATTGGACGCTGACTCGCCGATCGACGCACTGGCGCGTTTGCTGG
TCGAGCGGGACCGGTGCGAC

Product: prepilin peptidase and N-methyltransferase O

Products: NA

Alternate protein names: Leader peptidase; Prepilin peptidase; N-methyltransferase [H]

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MLHLPVLPLLLSPFIGSFLGVLAARLPRRLPVALARSACPRCGAVLRPRELVPVVSYLVQKGRCRSCGGAISAGHPAMEV
AALLVAGLVLLAAGPLRAGTPAPLLVVWSGCLFGWWLLVLAAIDLKSFRLPDTLTVPLIAAGLGLAALGGAAPVLWHALA
AAAGYGVFRGISLLYRRARGHDGLGGGDAKLLAAGGAWLGPESLPHVVFLGAVLTLCAVAIARRGDVNRTLMVPFGPGLA
LAMWGIWLWQASLLRPDWLPA

Sequences:

>Translated_261_residues
MLHLPVLPLLLSPFIGSFLGVLAARLPRRLPVALARSACPRCGAVLRPRELVPVVSYLVQKGRCRSCGGAISAGHPAMEV
AALLVAGLVLLAAGPLRAGTPAPLLVVWSGCLFGWWLLVLAAIDLKSFRLPDTLTVPLIAAGLGLAALGGAAPVLWHALA
AAAGYGVFRGISLLYRRARGHDGLGGGDAKLLAAGGAWLGPESLPHVVFLGAVLTLCAVAIARRGDVNRTLMVPFGPGLA
LAMWGIWLWQASLLRPDWLPA
>Mature_261_residues
MLHLPVLPLLLSPFIGSFLGVLAARLPRRLPVALARSACPRCGAVLRPRELVPVVSYLVQKGRCRSCGGAISAGHPAMEV
AALLVAGLVLLAAGPLRAGTPAPLLVVWSGCLFGWWLLVLAAIDLKSFRLPDTLTVPLIAAGLGLAALGGAAPVLWHALA
AAAGYGVFRGISLLYRRARGHDGLGGGDAKLLAAGGAWLGPESLPHVVFLGAVLTLCAVAIARRGDVNRTLMVPFGPGLA
LAMWGIWLWQASLLRPDWLPA

Specific function: Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue [H]

COG id: COG1989

COG function: function code NOU; Type II secretory pathway, prepilin signal peptidase PulO and related peptidases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A24 family [H]

Homologues:

Organism=Escherichia coli, GI87082194, Length=258, Percent_Identity=31.0077519379845, Blast_Score=91, Evalue=7e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010627
- InterPro:   IPR014032
- InterPro:   IPR000045 [H]

Pfam domain/function: PF06750 DiS_P_DiS; PF01478 Peptidase_A24 [H]

EC number: =3.4.23.43 [H]

Molecular weight: Translated: 27114; Mature: 27114

Theoretical pI: Translated: 11.25; Mature: 11.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHLPVLPLLLSPFIGSFLGVLAARLPRRLPVALARSACPRCGAVLRPRELVPVVSYLVQ
CCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
KGRCRSCGGAISAGHPAMEVAALLVAGLVLLAAGPLRAGTPAPLLVVWSGCLFGWWLLVL
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHH
AAIDLKSFRLPDTLTVPLIAAGLGLAALGGAAPVLWHALAAAAGYGVFRGISLLYRRARG
HHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
HDGLGGGDAKLLAAGGAWLGPESLPHVVFLGAVLTLCAVAIARRGDVNRTLMVPFGPGLA
CCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHH
LAMWGIWLWQASLLRPDWLPA
HHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MLHLPVLPLLLSPFIGSFLGVLAARLPRRLPVALARSACPRCGAVLRPRELVPVVSYLVQ
CCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
KGRCRSCGGAISAGHPAMEVAALLVAGLVLLAAGPLRAGTPAPLLVVWSGCLFGWWLLVL
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHH
AAIDLKSFRLPDTLTVPLIAAGLGLAALGGAAPVLWHALAAAAGYGVFRGISLLYRRARG
HHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
HDGLGGGDAKLLAAGGAWLGPESLPHVVFLGAVLTLCAVAIARRGDVNRTLMVPFGPGLA
CCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHH
LAMWGIWLWQASLLRPDWLPA
HHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9826339 [H]