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The map label for this gene is lsdB [H]

Identifier: 162146302

GI number: 162146302

Start: 475544

End: 477148

Strand: Direct

Name: lsdB [H]

Synonym: GDI_0477

Alternate gene names: 162146302

Gene position: 475544-477148 (Clockwise)

Preceding gene: 162146301

Following gene: 162146304

Centisome position: 12.06

GC content: 67.6

Gene sequence:

>1605_bases
ATGACCATGACCGGGACAGTTCCGCGATCGGCCGGATGGCGCCGCCTGTCGCGGCGCTGCCTGCCGGCCGCGTTCGCCCT
GCTGTTTCCTCTCGGGGCGCCGCACGCGGCCGATACGCCCCAATGGCGGCCCGTCCTGCATTTCTCGCCGGCGGCGTACT
GGATGAACGATCCGAACGGGCCCATCCTTCTCGATGGCGTCTATCACCTGTTCTACCAGTACGCTCCCGGCAGCATGACA
TGGGGGCATCCGTCCTGGGGGCACGCGACCAGCACGGATCTGCTCCACTGGACGGAGCATGGCGTCGCGATCGCGGCGAC
ACCGGGCGAGGAGATCTTTTCCGGAAGCCTGGTGCCGGACCCGCTGAATCGCTCGGGTCTGGGGAGTACGGACGCGCCGC
CGCTGCTTGCCTTCCACACATCCGTGTTCCACGACAATCCGGCCCATCCCGACGGTACGCAGGCACAATCGGTGTCCGTC
AGCCATGACGGCGGTTTCACGTGGCGGCCGTACGCCCACAACCCCGTCCTGACGCTTCATCCGGACTCCCGGCAGTTCAG
GGACCCTTCGGTCTTCTGGTACCAGGATGGCGGCTGCTGGATCATGACGACCGTCGTGGGCGACGCCCAGCTGGTCAAGC
TCTACCGGTCCACCGACCTGCTGCACTGGTCGTTTCTCAGCGACTTCCAGCCCTCCGGCTATCGCAAGCCCGGCATGCTG
TGGGAAATGCCCACACTGGTTCCGCTGAAGCTGGACGGAAACCCGCGGGCGACCAGATGGGTCATGATCGTCAGCGTCAA
TCCGTGGAGTATCGCGGGCGGATCGGGCGTGCAGTATTTCGTGGGCCGGTTCGACGGCGTGACCTTCACGCCGGACGCGC
TGCCTCCGCCGGGGTCCGATCCGTCCCGCTATGACTGGCTCGACCACGGCGCCGACCAGTATGCGACGACCCTGTTCGCC
AATACCGGCTCCGGCGCCCCGGTGCTGATCGGGTGGATGGACAACTGGGACTACGCGACGGACCTTCCGACCGCGCCCTG
GCGGGGGCAGATGACCCTGCCGGTGGACATCGCGCTGAAGACCGTGGACGGACATCCCACCGTGATCCAGACCCCGACCC
GCGCGTACGAGGACATGGTCAGGAAAAAGGGAGTCGTGACCTACGGGGACCGGAACCTGCCGCAGGACGGGCGCGTGACG
ATCCCGTCCCGGGGCGAGGTCCTGGACATCCGCCTGGTGCTGCGGCGCGGCGGCGCCCGGCGCGCCGGCATCGTGGTACG
GGAAACGCCGGACGGCAGGACCGGCACGTCGGTCAGCTACGATTTCGTGGACGGTACCCTGACCGTGGATCGCGGCCGTT
CCGGCCTGGTCGGCTTCTCGCCCCGCTTCAGCACCCGGCATATCGCGTACCTGGCCGCGCCGGGCGGCGAAGTCGCGCTT
CATCTGGTCGTCGATCGCGCTTCGGTCGAACTGTTCGCCAATGATGGGGTGCTGCGCATGACCGACCTGATCTTCCCCCC
GGCGGGAAGCGACCGGATCTCGCTGTTTGCCGAAGGGGGCGGCGCCACCATTCACGGATTGCGGGTGGCGGTGCTGGCCC
GATGA

Upstream 100 bases:

>100_bases
CCAACAATGCCCAGGTGCTGGCGCAGGTTCGCCAATTCCTGAACCAGTAATGACGGGGTTCGACCAGCCCGCCCTCCGGC
GGGCTGGTCTTTCGATATTG

Downstream 100 bases:

>100_bases
AATCCCCGGTGAAATCCCGTCCGCTCCGGCGGCGTTTACGAGGGGTGAGGCGGCCTGAACAGCCGCCGCGCCCAGGACAG
GACAAGGGCCTGGTAGGCCT

Product: levanase

Products: NA

Alternate protein names: Beta-D-fructofuranosidase; Exo-beta-D-fructosidase; Exo-levanase [H]

Number of amino acids: Translated: 534; Mature: 533

Protein sequence:

>534_residues
MTMTGTVPRSAGWRRLSRRCLPAAFALLFPLGAPHAADTPQWRPVLHFSPAAYWMNDPNGPILLDGVYHLFYQYAPGSMT
WGHPSWGHATSTDLLHWTEHGVAIAATPGEEIFSGSLVPDPLNRSGLGSTDAPPLLAFHTSVFHDNPAHPDGTQAQSVSV
SHDGGFTWRPYAHNPVLTLHPDSRQFRDPSVFWYQDGGCWIMTTVVGDAQLVKLYRSTDLLHWSFLSDFQPSGYRKPGML
WEMPTLVPLKLDGNPRATRWVMIVSVNPWSIAGGSGVQYFVGRFDGVTFTPDALPPPGSDPSRYDWLDHGADQYATTLFA
NTGSGAPVLIGWMDNWDYATDLPTAPWRGQMTLPVDIALKTVDGHPTVIQTPTRAYEDMVRKKGVVTYGDRNLPQDGRVT
IPSRGEVLDIRLVLRRGGARRAGIVVRETPDGRTGTSVSYDFVDGTLTVDRGRSGLVGFSPRFSTRHIAYLAAPGGEVAL
HLVVDRASVELFANDGVLRMTDLIFPPAGSDRISLFAEGGGATIHGLRVAVLAR

Sequences:

>Translated_534_residues
MTMTGTVPRSAGWRRLSRRCLPAAFALLFPLGAPHAADTPQWRPVLHFSPAAYWMNDPNGPILLDGVYHLFYQYAPGSMT
WGHPSWGHATSTDLLHWTEHGVAIAATPGEEIFSGSLVPDPLNRSGLGSTDAPPLLAFHTSVFHDNPAHPDGTQAQSVSV
SHDGGFTWRPYAHNPVLTLHPDSRQFRDPSVFWYQDGGCWIMTTVVGDAQLVKLYRSTDLLHWSFLSDFQPSGYRKPGML
WEMPTLVPLKLDGNPRATRWVMIVSVNPWSIAGGSGVQYFVGRFDGVTFTPDALPPPGSDPSRYDWLDHGADQYATTLFA
NTGSGAPVLIGWMDNWDYATDLPTAPWRGQMTLPVDIALKTVDGHPTVIQTPTRAYEDMVRKKGVVTYGDRNLPQDGRVT
IPSRGEVLDIRLVLRRGGARRAGIVVRETPDGRTGTSVSYDFVDGTLTVDRGRSGLVGFSPRFSTRHIAYLAAPGGEVAL
HLVVDRASVELFANDGVLRMTDLIFPPAGSDRISLFAEGGGATIHGLRVAVLAR
>Mature_533_residues
TMTGTVPRSAGWRRLSRRCLPAAFALLFPLGAPHAADTPQWRPVLHFSPAAYWMNDPNGPILLDGVYHLFYQYAPGSMTW
GHPSWGHATSTDLLHWTEHGVAIAATPGEEIFSGSLVPDPLNRSGLGSTDAPPLLAFHTSVFHDNPAHPDGTQAQSVSVS
HDGGFTWRPYAHNPVLTLHPDSRQFRDPSVFWYQDGGCWIMTTVVGDAQLVKLYRSTDLLHWSFLSDFQPSGYRKPGMLW
EMPTLVPLKLDGNPRATRWVMIVSVNPWSIAGGSGVQYFVGRFDGVTFTPDALPPPGSDPSRYDWLDHGADQYATTLFAN
TGSGAPVLIGWMDNWDYATDLPTAPWRGQMTLPVDIALKTVDGHPTVIQTPTRAYEDMVRKKGVVTYGDRNLPQDGRVTI
PSRGEVLDIRLVLRRGGARRAGIVVRETPDGRTGTSVSYDFVDGTLTVDRGRSGLVGFSPRFSTRHIAYLAAPGGEVALH
LVVDRASVELFANDGVLRMTDLIFPPAGSDRISLFAEGGGATIHGLRVAVLAR

Specific function: Exo-fructosidase that can hydrolyze both levan and inulin, leading to the production of free fructose. Is also able to hydrolyze sucrose and to a small extent raffinose, but not melezitose, stachylose, cellobiose, maltose, and lactose [H]

COG id: COG1621

COG function: function code G; Beta-fructosidases (levanase/invertase)

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 32 family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6322029, Length=325, Percent_Identity=38.7692307692308, Blast_Score=208, Evalue=2e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008985
- InterPro:   IPR010496
- InterPro:   IPR001362
- InterPro:   IPR018053
- InterPro:   IPR013189
- InterPro:   IPR013148
- InterPro:   IPR011040 [H]

Pfam domain/function: PF06439 DUF1080; PF08244 Glyco_hydro_32C; PF00251 Glyco_hydro_32N [H]

EC number: =3.2.1.80 [H]

Molecular weight: Translated: 58368; Mature: 58236

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS00609 GLYCOSYL_HYDROL_F32

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMTGTVPRSAGWRRLSRRCLPAAFALLFPLGAPHAADTPQWRPVLHFSPAAYWMNDPNG
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCEEEECCCCC
PILLDGVYHLFYQYAPGSMTWGHPSWGHATSTDLLHWTEHGVAIAATPGEEIFSGSLVPD
CEEEHHHHEEHEEECCCCCCCCCCCCCCCCCCCEEEEECCCEEEEECCCHHHHCCCCCCC
PLNRSGLGSTDAPPLLAFHTSVFHDNPAHPDGTQAQSVSVSHDGGFTWRPYAHNPVLTLH
CCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEEECCCCEEECCCCCCCEEEEC
PDSRQFRDPSVFWYQDGGCWIMTTVVGDAQLVKLYRSTDLLHWSFLSDFQPSGYRKPGML
CCCCCCCCCEEEEEECCCEEEEEEECCCHHHHHHHHHCCEEEEEHHCCCCCCCCCCCCCE
WEMPTLVPLKLDGNPRATRWVMIVSVNPWSIAGGSGVQYFVGRFDGVTFTPDALPPPGSD
EECCCEEEEEECCCCCCEEEEEEEEECCCEECCCCCCEEEEEECCCEEECCCCCCCCCCC
PSRYDWLDHGADQYATTLFANTGSGAPVLIGWMDNWDYATDLPTAPWRGQMTLPVDIALK
CCCCCHHHCCCCCEEEEEEEECCCCCEEEEEEECCCCCCCCCCCCCCCCEEEEEEEEEEE
TVDGHPTVIQTPTRAYEDMVRKKGVVTYGDRNLPQDGRVTIPSRGEVLDIRLVLRRGGAR
EECCCCCEEECCHHHHHHHHHHCCEEEECCCCCCCCCCEECCCCCCEEEEEEEEECCCCC
RAGIVVRETPDGRTGTSVSYDFVDGTLTVDRGRSGLVGFSPRFSTRHIAYLAAPGGEVAL
CCCEEEEECCCCCCCCEEEEEEECCEEEEECCCCCCEECCCCCCCEEEEEEECCCCCEEE
HLVVDRASVELFANDGVLRMTDLIFPPAGSDRISLFAEGGGATIHGLRVAVLAR
EEEEECCEEEEEECCCEEEEEEEECCCCCCCEEEEEECCCCCEEEEEEEEEEEC
>Mature Secondary Structure 
TMTGTVPRSAGWRRLSRRCLPAAFALLFPLGAPHAADTPQWRPVLHFSPAAYWMNDPNG
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCEEEECCCCC
PILLDGVYHLFYQYAPGSMTWGHPSWGHATSTDLLHWTEHGVAIAATPGEEIFSGSLVPD
CEEEHHHHEEHEEECCCCCCCCCCCCCCCCCCCEEEEECCCEEEEECCCHHHHCCCCCCC
PLNRSGLGSTDAPPLLAFHTSVFHDNPAHPDGTQAQSVSVSHDGGFTWRPYAHNPVLTLH
CCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEEECCCCEEECCCCCCCEEEEC
PDSRQFRDPSVFWYQDGGCWIMTTVVGDAQLVKLYRSTDLLHWSFLSDFQPSGYRKPGML
CCCCCCCCCEEEEEECCCEEEEEEECCCHHHHHHHHHCCEEEEEHHCCCCCCCCCCCCCE
WEMPTLVPLKLDGNPRATRWVMIVSVNPWSIAGGSGVQYFVGRFDGVTFTPDALPPPGSD
EECCCEEEEEECCCCCCEEEEEEEEECCCEECCCCCCEEEEEECCCEEECCCCCCCCCCC
PSRYDWLDHGADQYATTLFANTGSGAPVLIGWMDNWDYATDLPTAPWRGQMTLPVDIALK
CCCCCHHHCCCCCEEEEEEEECCCCCEEEEEEECCCCCCCCCCCCCCCCEEEEEEEEEEE
TVDGHPTVIQTPTRAYEDMVRKKGVVTYGDRNLPQDGRVTIPSRGEVLDIRLVLRRGGAR
EECCCCCEEECCHHHHHHHHHHCCEEEECCCCCCCCCCEECCCCCCEEEEEEEEECCCCC
RAGIVVRETPDGRTGTSVSYDFVDGTLTVDRGRSGLVGFSPRFSTRHIAYLAAPGGEVAL
CCCEEEEECCCCCCCCEEEEEEECCEEEEECCCCCCEECCCCCCCEEEEEEECCCCCEEE
HLVVDRASVELFANDGVLRMTDLIFPPAGSDRISLFAEGGGATIHGLRVAVLAR
EEEEECCEEEEEECCCEEEEEEEECCCCCCCEEEEEECCCCCEEEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3112519; 3120151; 9141695; 9384377; 2117666 [H]