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The map label for this gene is ada [H]

Identifier: 162146295

GI number: 162146295

Start: 468869

End: 469999

Strand: Direct

Name: ada [H]

Synonym: GDI_0470

Alternate gene names: 162146295

Gene position: 468869-469999 (Clockwise)

Preceding gene: 162146294

Following gene: 162146296

Centisome position: 11.89

GC content: 70.56

Gene sequence:

>1131_bases
GTGCGCGACAGGTTTGGAAGGAACGCCCCGATGGTCGAGATGACAGACATGACGGAACGGGACCCGCGCTGGGCCCGCAT
CGTGGCACGGGACCGCCGCGCGGACGGCCTGTTCTGGTATTCGGTGCGCACGACCGGGGTCTATTGCCGCCCGTCCTGTC
CCTCCCGAATGGCAAAGCCCGGCAACGTGCGCCTGCATGACACGCTGGGGGCCGCACGGGCCGCGGGCTTCCGTCCCTGC
CGCCGCTGCAACCCGGACGGCGCATCGCCGCAGGATATCGGCCAGGCCCTGGTCGTCCAGGCCTGCCGGCTGATCGCGGC
GCGGGACGACATGCCGTCCATCGCCGACCTGGCCCGCGCGGTGGAACTGAGCCCCAGCCATTTCCACCGGCTGTTCCGGG
CCGAGACCGGCCTGACGCCCCATGCCTATGCCGCCGCCCATCGCGCCGGCCGCATCCGCGCGGAACTGCCCCGCGCGGAC
AGCGTGACCGAGGCGATTTTCGATGCCGGATACGGGTCCAGCGGACGGTTCTACGCCCAGGCGGCCGATCTGCTGGGCAT
GACACCGGCCCGCTATCGCGCCGGCGGCGCGGGCGAACGGATTCGCTTCGCCGTCGGGCAATGCAGCCTCGGCGCGATCC
TGGTCGCGTCCAGTGCCAGGGGCGTGGTCGCGATCCTGATCGACGACGATCCGAACACCCTGGTCCGCGACCTGCAGGCA
CGTTTTCCCCGCGCCGAGCTGATCGGCGGTGACGAGGAGTACGAACGGCATGTCGCACTGGTGGTGGGCTTCGTGGAGGC
ACCCGGAATCGGGCTGGACCTGCCGCTCGATATTCGCGGCACCGCGTTCCAGCATCGTGTCTGGAGCGCGCTGCGCGACA
TTCCGGCAGGGCAAACCGTCACCTATGCCGACATCGCGCGACGGATCGGCCAGCCCCAGGCTGTCCGGGCGGTCGCCGGG
GCCTGCGCGGCCAACCGGATTGCCGTGGCCATTCCCTGCCATCGCGTCGTCCGCAACGACGGCGCACTGTCGGGCTATCG
CTGGGGGGTGGACCGCAAGCGCATCCTGATCGATCGCGAGCGCACGGCCGCGCCGGTTCCTTCCGCCCGTCCAGAAGGCC
GATCGTCTTGA

Upstream 100 bases:

>100_bases
GAGGACCTGCGCGGCGGCCGGTTCGGTACCCTCGGGGCCGCCTGAGCGGATTTGAAAGCAGGGGACGGAGTGCGCGGCCG
GTCGCCGTCCTGCATCGTGG

Downstream 100 bases:

>100_bases
CCGTGTGGAAGACCGAATGGGCGTCGTGACATTCCGCCAGCAGGTAATTGGGAATTCCCGGCGCGGCGTGGTGGACGTGA
TGCAGCCCGATGCTGGCGGA

Product: regulatory protein ada (regulatory protein of adaptative response)

Products: NA

Alternate protein names: Regulatory protein of adaptative response; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MRDRFGRNAPMVEMTDMTERDPRWARIVARDRRADGLFWYSVRTTGVYCRPSCPSRMAKPGNVRLHDTLGAARAAGFRPC
RRCNPDGASPQDIGQALVVQACRLIAARDDMPSIADLARAVELSPSHFHRLFRAETGLTPHAYAAAHRAGRIRAELPRAD
SVTEAIFDAGYGSSGRFYAQAADLLGMTPARYRAGGAGERIRFAVGQCSLGAILVASSARGVVAILIDDDPNTLVRDLQA
RFPRAELIGGDEEYERHVALVVGFVEAPGIGLDLPLDIRGTAFQHRVWSALRDIPAGQTVTYADIARRIGQPQAVRAVAG
ACAANRIAVAIPCHRVVRNDGALSGYRWGVDRKRILIDRERTAAPVPSARPEGRSS

Sequences:

>Translated_376_residues
MRDRFGRNAPMVEMTDMTERDPRWARIVARDRRADGLFWYSVRTTGVYCRPSCPSRMAKPGNVRLHDTLGAARAAGFRPC
RRCNPDGASPQDIGQALVVQACRLIAARDDMPSIADLARAVELSPSHFHRLFRAETGLTPHAYAAAHRAGRIRAELPRAD
SVTEAIFDAGYGSSGRFYAQAADLLGMTPARYRAGGAGERIRFAVGQCSLGAILVASSARGVVAILIDDDPNTLVRDLQA
RFPRAELIGGDEEYERHVALVVGFVEAPGIGLDLPLDIRGTAFQHRVWSALRDIPAGQTVTYADIARRIGQPQAVRAVAG
ACAANRIAVAIPCHRVVRNDGALSGYRWGVDRKRILIDRERTAAPVPSARPEGRSS
>Mature_376_residues
MRDRFGRNAPMVEMTDMTERDPRWARIVARDRRADGLFWYSVRTTGVYCRPSCPSRMAKPGNVRLHDTLGAARAAGFRPC
RRCNPDGASPQDIGQALVVQACRLIAARDDMPSIADLARAVELSPSHFHRLFRAETGLTPHAYAAAHRAGRIRAELPRAD
SVTEAIFDAGYGSSGRFYAQAADLLGMTPARYRAGGAGERIRFAVGQCSLGAILVASSARGVVAILIDDDPNTLVRDLQA
RFPRAELIGGDEEYERHVALVVGFVEAPGIGLDLPLDIRGTAFQHRVWSALRDIPAGQTVTYADIARRIGQPQAVRAVAG
ACAANRIAVAIPCHRVVRNDGALSGYRWGVDRKRILIDRERTAAPVPSARPEGRSS

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus. It activates the transcription of alkA, alkB and aidB [H]

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788542, Length=350, Percent_Identity=48.8571428571429, Blast_Score=338, Evalue=2e-94,
Organism=Escherichia coli, GI1787596, Length=87, Percent_Identity=44.8275862068966, Blast_Score=86, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI115533070, Length=80, Percent_Identity=57.5, Blast_Score=101, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI115533068, Length=80, Percent_Identity=57.5, Blast_Score=100, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6320001, Length=89, Percent_Identity=42.6966292134831, Blast_Score=95, Evalue=2e-20,
Organism=Drosophila melanogaster, GI17137554, Length=165, Percent_Identity=39.3939393939394, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR016221
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF01035 DNA_binding_1; PF00165 HTH_AraC; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 40897; Mature: 40897

Theoretical pI: Translated: 10.13; Mature: 10.13

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2 ; PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDRFGRNAPMVEMTDMTERDPRWARIVARDRRADGLFWYSVRTTGVYCRPSCPSRMAKP
CCCCCCCCCCEEEECCCCCCCCHHHHHHHHCCCCCCEEEEEEEECEEEECCCCHHHHCCC
GNVRLHDTLGAARAAGFRPCRRCNPDGASPQDIGQALVVQACRLIAARDDMPSIADLARA
CCEEEEHHHHHHHHCCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
VELSPSHFHRLFRAETGLTPHAYAAAHRAGRIRAELPRADSVTEAIFDAGYGSSGRFYAQ
HCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCEECCCCCCHHHHHHHHCCCCCCCCCHHH
AADLLGMTPARYRAGGAGERIRFAVGQCSLGAILVASSARGVVAILIDDDPNTLVRDLQA
HHHHHCCCCHHHCCCCCCCEEEEEECCCCCCEEEEECCCCCEEEEEEECCCHHHHHHHHH
RFPRAELIGGDEEYERHVALVVGFVEAPGIGLDLPLDIRGTAFQHRVWSALRDIPAGQTV
HCCHHHHCCCCHHHHHEEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEE
TYADIARRIGQPQAVRAVAGACAANRIAVAIPCHRVVRNDGALSGYRWGVDRKRILIDRE
EHHHHHHHHCCCHHHHHHHHHHHCCCEEEEECHHHHHHCCCCCCCEEECCCCCEEEEECC
RTAAPVPSARPEGRSS
CCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRDRFGRNAPMVEMTDMTERDPRWARIVARDRRADGLFWYSVRTTGVYCRPSCPSRMAKP
CCCCCCCCCCEEEECCCCCCCCHHHHHHHHCCCCCCEEEEEEEECEEEECCCCHHHHCCC
GNVRLHDTLGAARAAGFRPCRRCNPDGASPQDIGQALVVQACRLIAARDDMPSIADLARA
CCEEEEHHHHHHHHCCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
VELSPSHFHRLFRAETGLTPHAYAAAHRAGRIRAELPRADSVTEAIFDAGYGSSGRFYAQ
HCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCEECCCCCCHHHHHHHHCCCCCCCCCHHH
AADLLGMTPARYRAGGAGERIRFAVGQCSLGAILVASSARGVVAILIDDDPNTLVRDLQA
HHHHHCCCCHHHCCCCCCCEEEEEECCCCCCEEEEECCCCCEEEEEEECCCHHHHHHHHH
RFPRAELIGGDEEYERHVALVVGFVEAPGIGLDLPLDIRGTAFQHRVWSALRDIPAGQTV
HCCHHHHCCCCHHHHHEEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEE
TYADIARRIGQPQAVRAVAGACAANRIAVAIPCHRVVRNDGALSGYRWGVDRKRILIDRE
EHHHHHHHHCCCHHHHHHHHHHHCCCEEEEECHHHHHHCCCCCCCEEECCCCCEEEEECC
RTAAPVPSARPEGRSS
CCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2987251; 3887409; 9097040; 9278503; 2982792; 3536913; 3009022; 3529081; 1581309; 8202360; 8500619; 8156986 [H]