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The map label for this gene is gsiB [H]

Identifier: 162146288

GI number: 162146288

Start: 460848

End: 462536

Strand: Direct

Name: gsiB [H]

Synonym: GDI_0463

Alternate gene names: 162146288

Gene position: 460848-462536 (Clockwise)

Preceding gene: 162146285

Following gene: 162146289

Centisome position: 11.68

GC content: 65.9

Gene sequence:

>1689_bases
GTGAAGCACGTGCTGCCGTGCGACACGCTCTGCCGCGAACGGGGGACGAAACGACAGAATCTCCTCTCTCCGGCCGCGAA
GACGCGCTCCCTTATCGTTACGAACGGGTTATGCTTTCAAAAATCGCGCTGGAGGAGCTGTAACCCGGTGGCTTTCCGCT
CTCATACGCGCCGCGACGCGCTTCAGATCCTGGCCGGGGGCGCTATCGCGGCTTGCGCGCCGCCGGGCGCGCGGGCGCAG
GCGGTGGAAATCCCCCGGCGCGGGGGGCGCATCCGCGTCGCCGGCTTTTCCGGGTCCAGCGCCGATACGCTCGACCCCGC
GCGCGGGGCGCTGTCCACGGATTATATCCGTGGCGCGATGTTCTATGACGCGCTGACGGAACTGGACGAGGCGCTGCAGG
TACAGCCCTCCCTGGCCATTGCGATCGAAAGCGACGACGCCATCCGCTGGACGATCCGGCTGCGCAAGGGGGTCCGCTTT
CATGACGGCTCCCCGCTGACGGCGGACGATGTGGTGTTTTCGCTGCTGCGGCATCTCGACCCGCGCGTGGGCTCGCAGCA
GAAGGCGATCGCCCGGCAGTTCGGCAGCATCCGCGCGCGTGCCGCCGACGAGGTCGAGTTGACGCTGGTCGCGGCCAATG
TCGATCTTCCGGCCCTTCTGTCCCTGGCGCCGTTCTACATCATCAAGAACGCCACGACCGATTTTTCGCGCGCCAACGGC
ACGGGCCCGTTCCTCTGCCAGGAATTCAGCCCCGGCATCCGCTCGGTCGCCGTGCGCAATCCGGACTATTGGCGCGACGG
ACAGCCCCACCTGGACGAAATCGAATTTTTCACCATCGCCGATGACATGGCGCGCCATGATGCGCTGATGTCCGGGGACG
TCGACCTGATCGGCGGCGTCAATCCGCGGCTGGCGCCCCTGCTGCGGCAGCGCGGCCTGCAGATCATGGAAGCGCCGGGC
GGGGCCTATACCGATTTCATCATGCGGCTGGACCAGGCGCCTGGCAATAACCCGGATTTCGTGCGCGGAATGAAATACCT
GTTCAATCGCGAGCAGATGAAAAGCGCGATCTTCCGGGGCTACGCGCAGGTCGGGAACGACCAGCCGATCGCACCGGGGT
TTCCCTATTTCGATGCGTCGCTGCCCCAGACCGTGCAGGACCTGGACCGCGCGCGCTATCATTTCCGGCGATCCGGCCTG
ACGGGCAGCCGGGTGCCGATGGTCTGTTCGCCGGCGGCGGAGGGATCGGTCGAAATGGCGATCCTGTTGCAGCACGATGC
GCCGCCGCTGGGCATCGACCTGGCCATCCAGCAGGTCCCGGCGGACGGGTACTGGTCGAATTACTGGATGCAGGCGCCGA
TATCCTTCGGCAATCTGAACCCCCGGCCCCGGGCCGAAATGGCGTTTTCGCTGTCCTATGCCTCGGACGCGCCGTGGAAC
GAATCCCGCTGGCGCAATCCTGAATTCGACCGGCTGCTGCGCGCCGCGCGGGGCGAGCGCAATGAAAGCCTGCGGGCCGA
GATGTTCGCGCAAATGCAGGTCCTGGTCCATGACGGCAGCGGGGTGTGCATTCCGCTGTTCCTGAGTGATATCGACGCGT
TTTCACCCCGGCTGCGCGGCATGCGGCCCAGGAAGACGGGGGAATTCATGGGCTTCGAATTCGCCCGCCATGTCTGGCTG
GCGTCATGA

Upstream 100 bases:

>100_bases
TCCCCCGCGTTCCCGTTTGTCGATTTGTCCCACGGATGCGGCAAGGCGGCGTTGCGAATGTGGACGGCATTTCGATAGAT
CATACCGCAATCGTTCCGCC

Downstream 100 bases:

>100_bases
ACCGGTTTTCGCGCCACCTGATCGTGCTGCGGCTGGCGGGCCTGCGCATCCTGTCCGCGCTGGGCACGCTCCTGCTGGTG
ACCATCCTGATCTTCGCCGC

Product: extracellular solute-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 562; Mature: 562

Protein sequence:

>562_residues
MKHVLPCDTLCRERGTKRQNLLSPAAKTRSLIVTNGLCFQKSRWRSCNPVAFRSHTRRDALQILAGGAIAACAPPGARAQ
AVEIPRRGGRIRVAGFSGSSADTLDPARGALSTDYIRGAMFYDALTELDEALQVQPSLAIAIESDDAIRWTIRLRKGVRF
HDGSPLTADDVVFSLLRHLDPRVGSQQKAIARQFGSIRARAADEVELTLVAANVDLPALLSLAPFYIIKNATTDFSRANG
TGPFLCQEFSPGIRSVAVRNPDYWRDGQPHLDEIEFFTIADDMARHDALMSGDVDLIGGVNPRLAPLLRQRGLQIMEAPG
GAYTDFIMRLDQAPGNNPDFVRGMKYLFNREQMKSAIFRGYAQVGNDQPIAPGFPYFDASLPQTVQDLDRARYHFRRSGL
TGSRVPMVCSPAAEGSVEMAILLQHDAPPLGIDLAIQQVPADGYWSNYWMQAPISFGNLNPRPRAEMAFSLSYASDAPWN
ESRWRNPEFDRLLRAARGERNESLRAEMFAQMQVLVHDGSGVCIPLFLSDIDAFSPRLRGMRPRKTGEFMGFEFARHVWL
AS

Sequences:

>Translated_562_residues
MKHVLPCDTLCRERGTKRQNLLSPAAKTRSLIVTNGLCFQKSRWRSCNPVAFRSHTRRDALQILAGGAIAACAPPGARAQ
AVEIPRRGGRIRVAGFSGSSADTLDPARGALSTDYIRGAMFYDALTELDEALQVQPSLAIAIESDDAIRWTIRLRKGVRF
HDGSPLTADDVVFSLLRHLDPRVGSQQKAIARQFGSIRARAADEVELTLVAANVDLPALLSLAPFYIIKNATTDFSRANG
TGPFLCQEFSPGIRSVAVRNPDYWRDGQPHLDEIEFFTIADDMARHDALMSGDVDLIGGVNPRLAPLLRQRGLQIMEAPG
GAYTDFIMRLDQAPGNNPDFVRGMKYLFNREQMKSAIFRGYAQVGNDQPIAPGFPYFDASLPQTVQDLDRARYHFRRSGL
TGSRVPMVCSPAAEGSVEMAILLQHDAPPLGIDLAIQQVPADGYWSNYWMQAPISFGNLNPRPRAEMAFSLSYASDAPWN
ESRWRNPEFDRLLRAARGERNESLRAEMFAQMQVLVHDGSGVCIPLFLSDIDAFSPRLRGMRPRKTGEFMGFEFARHVWL
AS
>Mature_562_residues
MKHVLPCDTLCRERGTKRQNLLSPAAKTRSLIVTNGLCFQKSRWRSCNPVAFRSHTRRDALQILAGGAIAACAPPGARAQ
AVEIPRRGGRIRVAGFSGSSADTLDPARGALSTDYIRGAMFYDALTELDEALQVQPSLAIAIESDDAIRWTIRLRKGVRF
HDGSPLTADDVVFSLLRHLDPRVGSQQKAIARQFGSIRARAADEVELTLVAANVDLPALLSLAPFYIIKNATTDFSRANG
TGPFLCQEFSPGIRSVAVRNPDYWRDGQPHLDEIEFFTIADDMARHDALMSGDVDLIGGVNPRLAPLLRQRGLQIMEAPG
GAYTDFIMRLDQAPGNNPDFVRGMKYLFNREQMKSAIFRGYAQVGNDQPIAPGFPYFDASLPQTVQDLDRARYHFRRSGL
TGSRVPMVCSPAAEGSVEMAILLQHDAPPLGIDLAIQQVPADGYWSNYWMQAPISFGNLNPRPRAEMAFSLSYASDAPWN
ESRWRNPEFDRLLRAARGERNESLRAEMFAQMQVLVHDGSGVCIPLFLSDIDAFSPRLRGMRPRKTGEFMGFEFARHVWL
AS

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=273, Percent_Identity=26.3736263736264, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1787762, Length=460, Percent_Identity=24.5652173913043, Blast_Score=91, Evalue=3e-19,
Organism=Escherichia coli, GI1789887, Length=308, Percent_Identity=28.2467532467532, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1789966, Length=475, Percent_Identity=23.5789473684211, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI87081878, Length=250, Percent_Identity=25.6, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 62323; Mature: 62323

Theoretical pI: Translated: 8.55; Mature: 8.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHVLPCDTLCRERGTKRQNLLSPAAKTRSLIVTNGLCFQKSRWRSCNPVAFRSHTRRDA
CCCCCCHHHHHHHCCCCHHHHHCHHHHCCEEEEECCEEECCCCCCCCCCHHHHHHHHHHH
LQILAGGAIAACAPPGARAQAVEIPRRGGRIRVAGFSGSSADTLDPARGALSTDYIRGAM
HHHHCCCCEEECCCCCCCCEEEECCCCCCEEEEEECCCCCCCCCCCHHCCCCHHHHHHHH
FYDALTELDEALQVQPSLAIAIESDDAIRWTIRLRKGVRFHDGSPLTADDVVFSLLRHLD
HHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEEEECCCEECCCCCCCHHHHHHHHHHHCC
PRVGSQQKAIARQFGSIRARAADEVELTLVAANVDLPALLSLAPFYIIKNATTDFSRANG
CCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHCCEEEEECCCCCCCCCCC
TGPFLCQEFSPGIRSVAVRNPDYWRDGQPHLDEIEFFTIADDMARHDALMSGDVDLIGGV
CCCEEECCCCCCCCEEEECCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHCCCCCEECCC
NPRLAPLLRQRGLQIMEAPGGAYTDFIMRLDQAPGNNPDFVRGMKYLFNREQMKSAIFRG
CHHHHHHHHHCCCEEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCHHHHHHHHHHH
YAQVGNDQPIAPGFPYFDASLPQTVQDLDRARYHFRRSGLTGSRVPMVCSPAAEGSVEMA
HHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCEEEE
ILLQHDAPPLGIDLAIQQVPADGYWSNYWMQAPISFGNLNPRPRAEMAFSLSYASDAPWN
EEEECCCCCCCCEEEEEECCCCCCCCCEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCC
ESRWRNPEFDRLLRAARGERNESLRAEMFAQMQVLVHDGSGVCIPLFLSDIDAFSPRLRG
CCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHEEEEEECCCCCEEEHHHHHHHHHCHHHCC
MRPRKTGEFMGFEFARHVWLAS
CCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MKHVLPCDTLCRERGTKRQNLLSPAAKTRSLIVTNGLCFQKSRWRSCNPVAFRSHTRRDA
CCCCCCHHHHHHHCCCCHHHHHCHHHHCCEEEEECCEEECCCCCCCCCCHHHHHHHHHHH
LQILAGGAIAACAPPGARAQAVEIPRRGGRIRVAGFSGSSADTLDPARGALSTDYIRGAM
HHHHCCCCEEECCCCCCCCEEEECCCCCCEEEEEECCCCCCCCCCCHHCCCCHHHHHHHH
FYDALTELDEALQVQPSLAIAIESDDAIRWTIRLRKGVRFHDGSPLTADDVVFSLLRHLD
HHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEEEECCCEECCCCCCCHHHHHHHHHHHCC
PRVGSQQKAIARQFGSIRARAADEVELTLVAANVDLPALLSLAPFYIIKNATTDFSRANG
CCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHCCEEEEECCCCCCCCCCC
TGPFLCQEFSPGIRSVAVRNPDYWRDGQPHLDEIEFFTIADDMARHDALMSGDVDLIGGV
CCCEEECCCCCCCCEEEECCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHCCCCCEECCC
NPRLAPLLRQRGLQIMEAPGGAYTDFIMRLDQAPGNNPDFVRGMKYLFNREQMKSAIFRG
CHHHHHHHHHCCCEEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCHHHHHHHHHHH
YAQVGNDQPIAPGFPYFDASLPQTVQDLDRARYHFRRSGLTGSRVPMVCSPAAEGSVEMA
HHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCEEEE
ILLQHDAPPLGIDLAIQQVPADGYWSNYWMQAPISFGNLNPRPRAEMAFSLSYASDAPWN
EEEECCCCCCCCEEEEEECCCCCCCCCEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCC
ESRWRNPEFDRLLRAARGERNESLRAEMFAQMQVLVHDGSGVCIPLFLSDIDAFSPRLRG
CCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHEEEEEECCCCCEEEHHHHHHHHHCHHHCC
MRPRKTGEFMGFEFARHVWLAS
CCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA