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The map label for this gene is rpoN [H]

Identifier: 162146249

GI number: 162146249

Start: 422874

End: 424283

Strand: Reverse

Name: rpoN [H]

Synonym: GDI_0424

Alternate gene names: 162146249

Gene position: 424283-422874 (Counterclockwise)

Preceding gene: 162146250

Following gene: 162146246

Centisome position: 10.76

GC content: 67.94

Gene sequence:

>1410_bases
ATGACGCCGATGTCGGCCCAGCCCCGTCAGGAATTCAAGCAGAACCAGGGACTGTTCATGAGCGCGCGCATGCGCCAGTC
CATCCAGATCCTGCAATTGTCCAATGCCGAGCTCCATGAATTCCTGGACGCGGAGGTCGAGAAGAATCCGCTTCTGGAGC
GCGATCCGACCACCTCCGCCCCCCTGGCGGAGAGGCCCGGCCTTGCCGCGCCGCCGCCCATGATGCGCGCGCCCGGCGCG
ACGGGGGATGCCGGGCGCTGGACGTCCGACAGCGGCCTGGATGATGACGGGACGGGGCGCCTGGCCGAATCCGCCCCGGC
GTTGCGCGACGTGATGTTCGAACAACTGGTTCTGTCCGGCTGCACGACGACGGAGCGGGAAATCGGCGCGCACCTGATCG
CCGCACTCGATCCGGCGGGACGGCTGGCCGGCGGCGCCACCGACGACATCGCCGCCACGTTGCAGGTCACGCTGCGGGAC
GTCGAGGCCATCCGGCAGCGCATGATGCGTTTCGACCCGGCCGGCCTGTTCGCCACGTCGCTTCGGGAATGCCTGGCGGC
CCAGTTGCAGGAACGCAACCGTTTCGGCCCGGCCATGGCGCGCCTGCTGGACAATCTGGACCTTCTGGCCCGGCACGACC
TGAAGCGCCTGCGCCAGGCCTGCGACGTCAGCGCCACGGACCTGGAGGACATGATTGCCGAATTACGCACACTCGACCCC
AAGCCCGGGGCGGAACATGGCAGCACCCTGACCGTCATCGTCCCGGACATCCTGATGGAGCAGACGGCCGAGGGCGGATG
GACGCTGGAGCTGAACCCCGAGAGCACCCCCCGTGTCGTGCTGAACAGCGCGCTGAGCACCCGCATGTCGCTGCGCGCCC
ATGGGGCGGAGCGGACATACCTGAATGACACTCTGACCAGCGCGAACTGGCTGATCCGCGCGCTGCAGCAGCGCAGCATG
ACCATCCTGCGCGTCTCGACCGAGATCCTGCGCCGGCAGGAGGACTTCCTGCAGCACGGTCCGCAGGCCCTGCAGCCGCT
CAACCTCCGGACGGTCGCCGAGGCGCTGAACATTCACGAAAGCACGGTCAGCCGCGTGACGGCGAACAAGTACGTCGCGA
CCCCGCGCGGCGTGCTGCCGCTGAAATTCTTCTTCGTGGCGGCCATGACCGGAAATGACGGCGAAATCCGCAGCAACATT
GCGATCCAGAGCGTGATCCGCCGCATGATCCAGGGCGAACGCCCCGATGCCGTCCTGTCCGACGAGGCGATTTCCCTGAC
CCTGCGCCGGCAGGGCATCGACATCGCACGCCGCACGGTCGCGAAATATCGCGAGGCGATGGGCTTTCCCAATTCCCTCC
AGCGGCTTCAGCGCGCCGCCGAGGCCGCCCCGCCCCCGAACCGGCGATAG

Upstream 100 bases:

>100_bases
CCGACATGGCGGACCGGCTGGACAACCGCCGGGGGTATCGCCCGGATCACACCCTGCCTGATGCCGCCGCGACGGATTGT
CGCGCGGCCAGATCGGAAGG

Downstream 100 bases:

>100_bases
GCGCCGGAGCGGAGGCGGGGCGGGAGCCGGATTCTATCCGATGAGCGGAAGCAGGCCGTCGATGATGCGATGGAGGCGGT
CGCAGAGCACGTCGATTTCC

Product: RNA polymerase sigma-54 factor 1

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MTPMSAQPRQEFKQNQGLFMSARMRQSIQILQLSNAELHEFLDAEVEKNPLLERDPTTSAPLAERPGLAAPPPMMRAPGA
TGDAGRWTSDSGLDDDGTGRLAESAPALRDVMFEQLVLSGCTTTEREIGAHLIAALDPAGRLAGGATDDIAATLQVTLRD
VEAIRQRMMRFDPAGLFATSLRECLAAQLQERNRFGPAMARLLDNLDLLARHDLKRLRQACDVSATDLEDMIAELRTLDP
KPGAEHGSTLTVIVPDILMEQTAEGGWTLELNPESTPRVVLNSALSTRMSLRAHGAERTYLNDTLTSANWLIRALQQRSM
TILRVSTEILRRQEDFLQHGPQALQPLNLRTVAEALNIHESTVSRVTANKYVATPRGVLPLKFFFVAAMTGNDGEIRSNI
AIQSVIRRMIQGERPDAVLSDEAISLTLRRQGIDIARRTVAKYREAMGFPNSLQRLQRAAEAAPPPNRR

Sequences:

>Translated_469_residues
MTPMSAQPRQEFKQNQGLFMSARMRQSIQILQLSNAELHEFLDAEVEKNPLLERDPTTSAPLAERPGLAAPPPMMRAPGA
TGDAGRWTSDSGLDDDGTGRLAESAPALRDVMFEQLVLSGCTTTEREIGAHLIAALDPAGRLAGGATDDIAATLQVTLRD
VEAIRQRMMRFDPAGLFATSLRECLAAQLQERNRFGPAMARLLDNLDLLARHDLKRLRQACDVSATDLEDMIAELRTLDP
KPGAEHGSTLTVIVPDILMEQTAEGGWTLELNPESTPRVVLNSALSTRMSLRAHGAERTYLNDTLTSANWLIRALQQRSM
TILRVSTEILRRQEDFLQHGPQALQPLNLRTVAEALNIHESTVSRVTANKYVATPRGVLPLKFFFVAAMTGNDGEIRSNI
AIQSVIRRMIQGERPDAVLSDEAISLTLRRQGIDIARRTVAKYREAMGFPNSLQRLQRAAEAAPPPNRR
>Mature_468_residues
TPMSAQPRQEFKQNQGLFMSARMRQSIQILQLSNAELHEFLDAEVEKNPLLERDPTTSAPLAERPGLAAPPPMMRAPGAT
GDAGRWTSDSGLDDDGTGRLAESAPALRDVMFEQLVLSGCTTTEREIGAHLIAALDPAGRLAGGATDDIAATLQVTLRDV
EAIRQRMMRFDPAGLFATSLRECLAAQLQERNRFGPAMARLLDNLDLLARHDLKRLRQACDVSATDLEDMIAELRTLDPK
PGAEHGSTLTVIVPDILMEQTAEGGWTLELNPESTPRVVLNSALSTRMSLRAHGAERTYLNDTLTSANWLIRALQQRSMT
ILRVSTEILRRQEDFLQHGPQALQPLNLRTVAEALNIHESTVSRVTANKYVATPRGVLPLKFFFVAAMTGNDGEIRSNIA
IQSVIRRMIQGERPDAVLSDEAISLTLRRQGIDIARRTVAKYREAMGFPNSLQRLQRAAEAAPPPNRR

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of the nitrogen fixation genes [H]

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=472, Percent_Identity=33.0508474576271, Blast_Score=258, Evalue=5e-70,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 51700; Mature: 51569

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS00717 SIGMA54_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPMSAQPRQEFKQNQGLFMSARMRQSIQILQLSNAELHEFLDAEVEKNPLLERDPTTSA
CCCCCCCHHHHHHHCCCCEEHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCC
PLAERPGLAAPPPMMRAPGATGDAGRWTSDSGLDDDGTGRLAESAPALRDVMFEQLVLSG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHC
CTTTEREIGAHLIAALDPAGRLAGGATDDIAATLQVTLRDVEAIRQRMMRFDPAGLFATS
CCCCHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
LRECLAAQLQERNRFGPAMARLLDNLDLLARHDLKRLRQACDVSATDLEDMIAELRTLDP
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCC
KPGAEHGSTLTVIVPDILMEQTAEGGWTLELNPESTPRVVLNSALSTRMSLRAHGAERTY
CCCCCCCCEEEEEEHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCHH
LNDTLTSANWLIRALQQRSMTILRVSTEILRRQEDFLQHGPQALQPLNLRTVAEALNIHE
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHH
STVSRVTANKYVATPRGVLPLKFFFVAAMTGNDGEIRSNIAIQSVIRRMIQGERPDAVLS
HHHHHHHHCCEECCCCCCHHHEEHHHEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCEEC
DEAISLTLRRQGIDIARRTVAKYREAMGFPNSLQRLQRAAEAAPPPNRR
CCHHEEEHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TPMSAQPRQEFKQNQGLFMSARMRQSIQILQLSNAELHEFLDAEVEKNPLLERDPTTSA
CCCCCCHHHHHHHCCCCEEHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCC
PLAERPGLAAPPPMMRAPGATGDAGRWTSDSGLDDDGTGRLAESAPALRDVMFEQLVLSG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHC
CTTTEREIGAHLIAALDPAGRLAGGATDDIAATLQVTLRDVEAIRQRMMRFDPAGLFATS
CCCCHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
LRECLAAQLQERNRFGPAMARLLDNLDLLARHDLKRLRQACDVSATDLEDMIAELRTLDP
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCC
KPGAEHGSTLTVIVPDILMEQTAEGGWTLELNPESTPRVVLNSALSTRMSLRAHGAERTY
CCCCCCCCEEEEEEHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCHH
LNDTLTSANWLIRALQQRSMTILRVSTEILRRQEDFLQHGPQALQPLNLRTVAEALNIHE
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHH
STVSRVTANKYVATPRGVLPLKFFFVAAMTGNDGEIRSNIAIQSVIRRMIQGERPDAVLS
HHHHHHHHCCEECCCCCCHHHEEHHHEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCEEC
DEAISLTLRRQGIDIARRTVAKYREAMGFPNSLQRLQRAAEAAPPPNRR
CCHHEEEHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1991712; 12597275 [H]