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The map label for this gene is iolD [H]

Identifier: 162146190

GI number: 162146190

Start: 374183

End: 376051

Strand: Direct

Name: iolD [H]

Synonym: GDI_0362

Alternate gene names: 162146190

Gene position: 374183-376051 (Clockwise)

Preceding gene: 162146189

Following gene: 162146191

Centisome position: 9.49

GC content: 70.25

Gene sequence:

>1869_bases
ATGAACACGATCCGCCTGACCATGGCACAGGCGCTGGTCCGCGCCATGTGCGCGCAGAAGACCGAGCTGGACGGCCAGAT
CGTGCCGTTCTTCGGCGGTGTCTGGGCCATTTTCGGCCACGGCAACGTCGCCGGCATGGGCGAGGCACTGCACGCCCAGC
GCGACGACATCACCACCTTCCGCGCCCATAACGAACAGGGCATGGTCCACGCCGCCACGGCCTATGCCAAGGCGCAGCGG
CGGCGGCGGGCCATGGCCTGCACCACCTCGATCGGCCCGGGCGCGCTGAACATGGTCACGGGCGCCGCCGTGGCGCACGT
GAACCGCCTGCCGGTGCTGCTGCTGCCCGGCGACGTGTTCGCCAACCGCGTGCCCGATCCCGTGCTGCAGCAGGTCGAGG
ATTTCGGGGACGGCACCATCACCGCCAATGACGCCTTCCGCCCGGTATCGCGCTATTTCGATCGCATCACCCGGCCGGAA
CAGCTCATTCCCGCCTTCGCCCGCGCCATGGCCGTGCTGACCGATCCCGCCGAATGCGGCCCGGCGACGCTCGCGCTGTG
CCAGGACGTGCAGGCGGAAGCGTTCGACTATCCCGAGCATGTCTTCGCCGAGCGCATCCACCGGCCCCGGCGCCCCGCCC
CCGACGAACGGGATCTGCATGACGCGGCCGGCATCATCCGCGCGGCGCGGCGGCCCTTCGTGGTGGTCGGCGGCGGCGTG
CCGTATTCCGGGGCAGGGGAGCAGGTGGCCGCCTTCTGCGCCGCGCGCGGCATTCCCGCCGGGGAAACCCAGGCGGGGAA
ATCCGCGCTGCCGGCCACGCTGGCCATGAACATGGGCGGCGTCGGCGTCAGCGGCACCAGCGCGGCCAACACCATGGCGG
CGCAGGCCGACGTGGTGATCGCCATCGGCACGCGGCTGCAGGATTTCACGACCGGGTCCTGGGGCCTGTTCCAGAACCCC
GACATGCGGATCGTGGGCCTGAACGTGCAGGCGTTCGACGCCATGAAGCACGAGGCGCAGCCGCTGCTGGCCGACGCGCG
CGTGGGGCTGGCGGCGCTGGACGCGCACCTGGGGGACTGGCGCGCGGAAGGCGACTGGACGGGCATGGCCGAACGCGCCC
GCGCCGAATGGCTGGAAATCTCGGCGCGCTGCCTGGCGGCATCTTCGGCCTCGGCCCCGCCATCGGGCGCCCTGCCGTCC
GACGCGCAGGTGATCGGCGCCGTGCGCCGCGCCGGCACGCCCACGGATGTCGTCGTCTGCGCGGCGGGGGGGCTTCCGGC
CGAACTGCAGAAGCACTGGCGGGCGGACAGGCCGGGCGGCTACCACATGGAGTACGGCTTTTCCTGCATGGGGTACGAAC
TGGCCGGCGCCCTCGGCGTCAAGATGGCCGATCCGTCGCGCGAGGTCATCGTGATGGTGGGGGACGGCTCCTATTTGATG
CTGAATTCCGAAATCGCGACCTCGGTGATGCTGGGGTTCAGGCTGACCATCGTCGTGCTGGACAATCACGGCTATGGCTG
CATCAACCGGTTGCAGCAGGCATGCGGCGGCGCGCCGTTCAACAATCTGTGGAAAAATTCGATGCATATGCACGAATTTC
CCGCCATCGACTTCGCGGCCCACGCGCGCAGCCTGGGCGCTGACGCCGTCAAGGTGTCCGGCCTGGCGGCGCTGGAGGAC
GCGGTCCGCCAGGCCCGCGACGCCGCCCGCACCACCGTCATCGTCATCGACACCGATCCCATGCCGACGACGCGGGACGG
CGGCGCGTGGTGGGACGTGGCGGTGCCCGAAATCTCCGCCCGCCCGGGCGTGAACCAGGCCCGCGCGGAATACGAATGCA
AGCGCAATTCCCAACGCATCGGAAACTGA

Upstream 100 bases:

>100_bases
ACGACGCAGGCGCGGTCGATGAAATGAGCCGCCGTTTCGCGCGCTTCGTCGCCGCCTGGCAGGCGGTGCCGCGCACCACC
GACGCAACGGGACAACGGTC

Downstream 100 bases:

>100_bases
CCATGTCCGCAAAAATCGGCGCCAACCCCATCATCTGGTCGAACGACGACATGCCGGAACTGGGGGGCGAGACCCCGCTG
GAAACCTGCCTGGCCGAGGC

Product: acetolactate synthase large subunit

Products: pyruvate; 2-aceto-2-hydroxy-butyrate; CO2 [C]

Alternate protein names: THcHDO hydrolase [H]

Number of amino acids: Translated: 622; Mature: 622

Protein sequence:

>622_residues
MNTIRLTMAQALVRAMCAQKTELDGQIVPFFGGVWAIFGHGNVAGMGEALHAQRDDITTFRAHNEQGMVHAATAYAKAQR
RRRAMACTTSIGPGALNMVTGAAVAHVNRLPVLLLPGDVFANRVPDPVLQQVEDFGDGTITANDAFRPVSRYFDRITRPE
QLIPAFARAMAVLTDPAECGPATLALCQDVQAEAFDYPEHVFAERIHRPRRPAPDERDLHDAAGIIRAARRPFVVVGGGV
PYSGAGEQVAAFCAARGIPAGETQAGKSALPATLAMNMGGVGVSGTSAANTMAAQADVVIAIGTRLQDFTTGSWGLFQNP
DMRIVGLNVQAFDAMKHEAQPLLADARVGLAALDAHLGDWRAEGDWTGMAERARAEWLEISARCLAASSASAPPSGALPS
DAQVIGAVRRAGTPTDVVVCAAGGLPAELQKHWRADRPGGYHMEYGFSCMGYELAGALGVKMADPSREVIVMVGDGSYLM
LNSEIATSVMLGFRLTIVVLDNHGYGCINRLQQACGGAPFNNLWKNSMHMHEFPAIDFAAHARSLGADAVKVSGLAALED
AVRQARDAARTTVIVIDTDPMPTTRDGGAWWDVAVPEISARPGVNQARAEYECKRNSQRIGN

Sequences:

>Translated_622_residues
MNTIRLTMAQALVRAMCAQKTELDGQIVPFFGGVWAIFGHGNVAGMGEALHAQRDDITTFRAHNEQGMVHAATAYAKAQR
RRRAMACTTSIGPGALNMVTGAAVAHVNRLPVLLLPGDVFANRVPDPVLQQVEDFGDGTITANDAFRPVSRYFDRITRPE
QLIPAFARAMAVLTDPAECGPATLALCQDVQAEAFDYPEHVFAERIHRPRRPAPDERDLHDAAGIIRAARRPFVVVGGGV
PYSGAGEQVAAFCAARGIPAGETQAGKSALPATLAMNMGGVGVSGTSAANTMAAQADVVIAIGTRLQDFTTGSWGLFQNP
DMRIVGLNVQAFDAMKHEAQPLLADARVGLAALDAHLGDWRAEGDWTGMAERARAEWLEISARCLAASSASAPPSGALPS
DAQVIGAVRRAGTPTDVVVCAAGGLPAELQKHWRADRPGGYHMEYGFSCMGYELAGALGVKMADPSREVIVMVGDGSYLM
LNSEIATSVMLGFRLTIVVLDNHGYGCINRLQQACGGAPFNNLWKNSMHMHEFPAIDFAAHARSLGADAVKVSGLAALED
AVRQARDAARTTVIVIDTDPMPTTRDGGAWWDVAVPEISARPGVNQARAEYECKRNSQRIGN
>Mature_622_residues
MNTIRLTMAQALVRAMCAQKTELDGQIVPFFGGVWAIFGHGNVAGMGEALHAQRDDITTFRAHNEQGMVHAATAYAKAQR
RRRAMACTTSIGPGALNMVTGAAVAHVNRLPVLLLPGDVFANRVPDPVLQQVEDFGDGTITANDAFRPVSRYFDRITRPE
QLIPAFARAMAVLTDPAECGPATLALCQDVQAEAFDYPEHVFAERIHRPRRPAPDERDLHDAAGIIRAARRPFVVVGGGV
PYSGAGEQVAAFCAARGIPAGETQAGKSALPATLAMNMGGVGVSGTSAANTMAAQADVVIAIGTRLQDFTTGSWGLFQNP
DMRIVGLNVQAFDAMKHEAQPLLADARVGLAALDAHLGDWRAEGDWTGMAERARAEWLEISARCLAASSASAPPSGALPS
DAQVIGAVRRAGTPTDVVVCAAGGLPAELQKHWRADRPGGYHMEYGFSCMGYELAGALGVKMADPSREVIVMVGDGSYLM
LNSEIATSVMLGFRLTIVVLDNHGYGCINRLQQACGGAPFNNLWKNSMHMHEFPAIDFAAHARSLGADAVKVSGLAALED
AVRQARDAARTTVIVIDTDPMPTTRDGGAWWDVAVPEISARPGVNQARAEYECKRNSQRIGN

Specific function: Involved in the cleavage of the C1-C2 bond of 3D- (3,5/4)-trihydroxycyclohexane-1,2-dione (THcHDO) to yield 5-deoxy- glucuronate (5DG) [H]

COG id: COG3962

COG function: function code E; Acetolactate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081685, Length=557, Percent_Identity=25.8527827648115, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1790104, Length=474, Percent_Identity=24.4725738396624, Blast_Score=119, Evalue=8e-28,
Organism=Escherichia coli, GI1787096, Length=497, Percent_Identity=23.1388329979879, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1786717, Length=531, Percent_Identity=23.9171374764595, Blast_Score=83, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6323755, Length=608, Percent_Identity=22.2039473684211, Blast_Score=109, Evalue=1e-24,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: 2.2.1.6 [C]

Molecular weight: Translated: 66152; Mature: 66152

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTIRLTMAQALVRAMCAQKTELDGQIVPFFGGVWAIFGHGNVAGMGEALHAQRDDITTF
CCEEEHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEEECCCCCCCCHHHHCCCCCCEEE
RAHNEQGMVHAATAYAKAQRRRRAMACTTSIGPGALNMVTGAAVAHVNRLPVLLLPGDVF
EECCCCCCEEHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHCCCCEEEECCHHH
ANRVPDPVLQQVEDFGDGTITANDAFRPVSRYFDRITRPEQLIPAFARAMAVLTDPAECG
HCCCCCHHHHHHHHCCCCEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHCC
PATLALCQDVQAEAFDYPEHVFAERIHRPRRPAPDERDLHDAAGIIRAARRPFVVVGGGV
HHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCC
PYSGAGEQVAAFCAARGIPAGETQAGKSALPATLAMNMGGVGVSGTSAANTMAAQADVVI
CCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHEEECCCCCCCCCCCHHHHHHHCCCEEE
AIGTRLQDFTTGSWGLFQNPDMRIVGLNVQAFDAMKHEAQPLLADARVGLAALDAHLGDW
EECCCCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHCCCHHHHHHCCHHHHHHHHCCC
RAEGDWTGMAERARAEWLEISARCLAASSASAPPSGALPSDAQVIGAVRRAGTPTDVVVC
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEE
AAGGLPAELQKHWRADRPGGYHMEYGFSCMGYELAGALGVKMADPSREVIVMVGDGSYLM
ECCCCCHHHHHHHCCCCCCCEEEECCCEEECHHHHHHHEEEECCCCCEEEEEECCCCEEE
LNSEIATSVMLGFRLTIVVLDNHGYGCINRLQQACGGAPFNNLWKNSMHMHEFPAIDFAA
EEHHHHHHHHHCEEEEEEEEECCCCHHHHHHHHHHCCCCHHHHHHCCCCHHHCCCHHHHH
HARSLGADAVKVSGLAALEDAVRQARDAARTTVIVIDTDPMPTTRDGGAWWDVAVPEISA
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCCCCCCCCEEEEECCCCCC
RPGVNQARAEYECKRNSQRIGN
CCCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNTIRLTMAQALVRAMCAQKTELDGQIVPFFGGVWAIFGHGNVAGMGEALHAQRDDITTF
CCEEEHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEEECCCCCCCCHHHHCCCCCCEEE
RAHNEQGMVHAATAYAKAQRRRRAMACTTSIGPGALNMVTGAAVAHVNRLPVLLLPGDVF
EECCCCCCEEHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHCCCCEEEECCHHH
ANRVPDPVLQQVEDFGDGTITANDAFRPVSRYFDRITRPEQLIPAFARAMAVLTDPAECG
HCCCCCHHHHHHHHCCCCEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHCC
PATLALCQDVQAEAFDYPEHVFAERIHRPRRPAPDERDLHDAAGIIRAARRPFVVVGGGV
HHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCC
PYSGAGEQVAAFCAARGIPAGETQAGKSALPATLAMNMGGVGVSGTSAANTMAAQADVVI
CCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHEEECCCCCCCCCCCHHHHHHHCCCEEE
AIGTRLQDFTTGSWGLFQNPDMRIVGLNVQAFDAMKHEAQPLLADARVGLAALDAHLGDW
EECCCCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHHHCCCHHHHHHCCHHHHHHHHCCC
RAEGDWTGMAERARAEWLEISARCLAASSASAPPSGALPSDAQVIGAVRRAGTPTDVVVC
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEE
AAGGLPAELQKHWRADRPGGYHMEYGFSCMGYELAGALGVKMADPSREVIVMVGDGSYLM
ECCCCCHHHHHHHCCCCCCCEEEECCCEEECHHHHHHHEEEECCCCCEEEEEECCCCEEE
LNSEIATSVMLGFRLTIVVLDNHGYGCINRLQQACGGAPFNNLWKNSMHMHEFPAIDFAA
EEHHHHHHHHHCEEEEEEEEECCCCHHHHHHHHHHCCCCHHHHHHCCCCHHHCCCHHHHH
HARSLGADAVKVSGLAALEDAVRQARDAARTTVIVIDTDPMPTTRDGGAWWDVAVPEISA
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCCCCCCCCEEEEECCCCCC
RPGVNQARAEYECKRNSQRIGN
CCCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 4002 [C]

Specific activity: 53

Km value (mM): 5 {2-oxobutanoate}} 1 {2-oxobutanoate}} 7.6 {pyruvate}} 7 {pyruvate}} 2.9 {pyruvate}} 2.6 {pyruvate}} 1.3 {pyruvate}} 1 {pyruvate}} [C]

Substrates: acetolactate; CO2; pyruvate; 2-oxobutanoate [C]

Specific reaction: acetolactate + CO2 = 2 pyruvate pyruvate + 2-oxobutanoate = 2-aceto-2-hydroxy-butyrate + CO2 [C]

General reaction: C-C bond formation; Decarboxylation [C]

Inhibitor: 2-oxobutanoate; chlorimuronethyl; chlorsulphuron; Ile; imazapyr; Leu; Val [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA