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The map label for this gene is citA [H]

Identifier: 162146174

GI number: 162146174

Start: 358418

End: 359710

Strand: Reverse

Name: citA [H]

Synonym: GDI_0346

Alternate gene names: 162146174

Gene position: 359710-358418 (Counterclockwise)

Preceding gene: 162146178

Following gene: 162146165

Centisome position: 9.12

GC content: 70.22

Gene sequence:

>1293_bases
ATGCGGCATTCATCGGCCATGACGACGCACCCCCCGACCGATATCCCGCGCATCAGGGCCGCCCTTCAGGTCGCCGCCGG
CAACATGCTGGAGATGTACGACTTCATGGTGTTCGGCTATTACGCCGCCGCCATCGGCCAGGCCTTCTTTCCGTCCGGCA
ACGCCTATGCCGAGACGCTGAAATCCTTCGCGACCTTCGGCGCGGGCTTTTTGATGCGCCCGCTGGGCGCGCTGATCCTG
GGCGCCTATGTGGACCGGATCGGGCGGCGGCGGGGCCTGCTGCTGACGCTGTTGCTGATGGCGATCGGCACGCTGACCCT
GACCGTCACCCCCCGCTATGCCGCGATCGGCCTGGCCGCGCCGGTCATCGTGCTGTGCGGGCGGCTGCTGCAGGGGTTTT
CGGCCGGCGTGGAACTGGGATCGACATCGGTCTACCTGGCGGAAATCGCGCGGCCCGCCCATCGCGGCTTCCTGGTCTCG
CTGCAATCGGCATCGCAGCAGGTGGCGGTGATCGGCGCGGCGGCGATCGGGGTGGCGCTGGCGACGATGCTGCCCGACGC
CACCATGCGGCAATGGGGCTGGCGGGTGCCGCTGCTGGCGGGCTGCCTGATCGTGCCGGTCCTGTTCGTCCTGCGCCGGC
ACCTGCGCGAGACCGCGGCCTTCGCCGCCCAGGCCGCCCCGCCCCACCTGCCCGAGGTGCTGGCCACCCTGCGCCGGGAA
TGGCGCGTGGTATCGACCGGCGTGATGATGGTGCTGATGACCACGATTTCGTTCTACGTCATCACGGCCTATACCCCGAC
CTTCGGCAGCGGCGTGCTGCATCTGTCGGGCCGCGACAGCCTGGCCGTCACGCTGTGCGTCGGTCTGTCGAACCTGCTGC
TGCTGCCGGCCTTCGGCGCGGTGTCGGACCGGGTCGGACGGCGTCCGCTGCTGGCCGGCGCCACGATCGCCGCCATCCTG
ACCGCCTGGCCCGCGATGTCGTGGCTGGTCGCGTCCCCGTCCTTCGCCCACCTGCTGGCGGTGGAGATCTGGCTGTCGCT
GATCTACAGCGCCTATAACGGCGCCGCCGTGGTGTACCTGACCGAGATCGTGCCGCCGCGCATCCGCACCACCGGCTTTT
CGCTGGCCTATAGCTGCGCGACCTGCATCGGCGGCTTCACGCCGGCGCTGTGCACGTGGCTGATCCATGTGACGGACAGC
CGCGCGGCGCCGGGGGCGGTGCTGTCCGCCGCCGCCCTGTGCGGACTGGCCGCCGCACTGGTCGCGCGGCCATTCCGGAA
AACGGAGGGGTAG

Upstream 100 bases:

>100_bases
GGAAGGTTCGGACACCATCCGCCCGACATGGCATGTATGAAGGTAAACTGCAAAGTTATTCTACACACCCGCCGGGCCGG
GGTGGACCGAAGGGATCGCG

Downstream 100 bases:

>100_bases
GCGCCCGCCCGAAGGCAGGCGCCCGCCCCCGCGGGCCGTCAGAACGGCGCATCGATGTCCACGACGTCGATCAGCTTGGC
ATTGACGAATTCCTTGATGC

Product: citrate-proton symporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter; Citrate utilization determinant; Citrate utilization protein A [H]

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MRHSSAMTTHPPTDIPRIRAALQVAAGNMLEMYDFMVFGYYAAAIGQAFFPSGNAYAETLKSFATFGAGFLMRPLGALIL
GAYVDRIGRRRGLLLTLLLMAIGTLTLTVTPRYAAIGLAAPVIVLCGRLLQGFSAGVELGSTSVYLAEIARPAHRGFLVS
LQSASQQVAVIGAAAIGVALATMLPDATMRQWGWRVPLLAGCLIVPVLFVLRRHLRETAAFAAQAAPPHLPEVLATLRRE
WRVVSTGVMMVLMTTISFYVITAYTPTFGSGVLHLSGRDSLAVTLCVGLSNLLLLPAFGAVSDRVGRRPLLAGATIAAIL
TAWPAMSWLVASPSFAHLLAVEIWLSLIYSAYNGAAVVYLTEIVPPRIRTTGFSLAYSCATCIGGFTPALCTWLIHVTDS
RAAPGAVLSAAALCGLAAALVARPFRKTEG

Sequences:

>Translated_430_residues
MRHSSAMTTHPPTDIPRIRAALQVAAGNMLEMYDFMVFGYYAAAIGQAFFPSGNAYAETLKSFATFGAGFLMRPLGALIL
GAYVDRIGRRRGLLLTLLLMAIGTLTLTVTPRYAAIGLAAPVIVLCGRLLQGFSAGVELGSTSVYLAEIARPAHRGFLVS
LQSASQQVAVIGAAAIGVALATMLPDATMRQWGWRVPLLAGCLIVPVLFVLRRHLRETAAFAAQAAPPHLPEVLATLRRE
WRVVSTGVMMVLMTTISFYVITAYTPTFGSGVLHLSGRDSLAVTLCVGLSNLLLLPAFGAVSDRVGRRPLLAGATIAAIL
TAWPAMSWLVASPSFAHLLAVEIWLSLIYSAYNGAAVVYLTEIVPPRIRTTGFSLAYSCATCIGGFTPALCTWLIHVTDS
RAAPGAVLSAAALCGLAAALVARPFRKTEG
>Mature_430_residues
MRHSSAMTTHPPTDIPRIRAALQVAAGNMLEMYDFMVFGYYAAAIGQAFFPSGNAYAETLKSFATFGAGFLMRPLGALIL
GAYVDRIGRRRGLLLTLLLMAIGTLTLTVTPRYAAIGLAAPVIVLCGRLLQGFSAGVELGSTSVYLAEIARPAHRGFLVS
LQSASQQVAVIGAAAIGVALATMLPDATMRQWGWRVPLLAGCLIVPVLFVLRRHLRETAAFAAQAAPPHLPEVLATLRRE
WRVVSTGVMMVLMTTISFYVITAYTPTFGSGVLHLSGRDSLAVTLCVGLSNLLLLPAFGAVSDRVGRRPLLAGATIAAIL
TAWPAMSWLVASPSFAHLLAVEIWLSLIYSAYNGAAVVYLTEIVPPRIRTTGFSLAYSCATCIGGFTPALCTWLIHVTDS
RAAPGAVLSAAALCGLAAALVARPFRKTEG

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=424, Percent_Identity=29.2452830188679, Blast_Score=207, Evalue=9e-55,
Organism=Escherichia coli, GI1788942, Length=379, Percent_Identity=31.9261213720317, Blast_Score=171, Evalue=1e-43,
Organism=Escherichia coli, GI1788292, Length=387, Percent_Identity=31.266149870801, Blast_Score=165, Evalue=4e-42,
Organism=Escherichia coli, GI1789941, Length=399, Percent_Identity=29.5739348370927, Blast_Score=132, Evalue=4e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 45518; Mature: 45518

Theoretical pI: Translated: 10.02; Mature: 10.02

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHSSAMTTHPPTDIPRIRAALQVAAGNMLEMYDFMVFGYYAAAIGQAFFPSGNAYAETL
CCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
KSFATFGAGFLMRPLGALILGAYVDRIGRRRGLLLTLLLMAIGTLTLTVTPRYAAIGLAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEEEECCCHHHHHHHH
PVIVLCGRLLQGFSAGVELGSTSVYLAEIARPAHRGFLVSLQSASQQVAVIGAAAIGVAL
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCHHHCCEEEEECCCCCHHHHHHHHHHHHHH
ATMLPDATMRQWGWRVPLLAGCLIVPVLFVLRRHLRETAAFAAQAAPPHLPEVLATLRRE
HHHCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
WRVVSTGVMMVLMTTISFYVITAYTPTFGSGVLHLSGRDSLAVTLCVGLSNLLLLPAFGA
HHHHHHHHHHHHHHHHHHHHHEEECCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCHH
VSDRVGRRPLLAGATIAAILTAWPAMSWLVASPSFAHLLAVEIWLSLIYSAYNGAAVVYL
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEEEE
TEIVPPRIRTTGFSLAYSCATCIGGFTPALCTWLIHVTDSRAAPGAVLSAAALCGLAAAL
ECCCCCCHHHCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
VARPFRKTEG
HHCCHHCCCC
>Mature Secondary Structure
MRHSSAMTTHPPTDIPRIRAALQVAAGNMLEMYDFMVFGYYAAAIGQAFFPSGNAYAETL
CCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
KSFATFGAGFLMRPLGALILGAYVDRIGRRRGLLLTLLLMAIGTLTLTVTPRYAAIGLAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEEEECCCHHHHHHHH
PVIVLCGRLLQGFSAGVELGSTSVYLAEIARPAHRGFLVSLQSASQQVAVIGAAAIGVAL
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCHHHCCEEEEECCCCCHHHHHHHHHHHHHH
ATMLPDATMRQWGWRVPLLAGCLIVPVLFVLRRHLRETAAFAAQAAPPHLPEVLATLRRE
HHHCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
WRVVSTGVMMVLMTTISFYVITAYTPTFGSGVLHLSGRDSLAVTLCVGLSNLLLLPAFGA
HHHHHHHHHHHHHHHHHHHHHEEECCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCHH
VSDRVGRRPLLAGATIAAILTAWPAMSWLVASPSFAHLLAVEIWLSLIYSAYNGAAVVYL
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEEEE
TEIVPPRIRTTGFSLAYSCATCIGGFTPALCTWLIHVTDSRAAPGAVLSAAALCGLAAAL
ECCCCCCHHHCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
VARPFRKTEG
HHCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]