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The map label for this gene is arsB [H]

Identifier: 162146163

GI number: 162146163

Start: 346388

End: 347641

Strand: Direct

Name: arsB [H]

Synonym: GDI_0335

Alternate gene names: 162146163

Gene position: 346388-347641 (Clockwise)

Preceding gene: 162146162

Following gene: 162146166

Centisome position: 8.78

GC content: 58.21

Gene sequence:

>1254_bases
ATGACCTTTCACCAAGAGGCGATCTGGGCAATCGCGGCTCTGGCGACGGCCGGTGTCATAATCCAACCGTTCCGGGTTCC
GGAGTGGGTATGGGCCGTCATAGGGGCGGCAGCCCTCGTGCTTACGGGGCTGATCCCCCTGACCATGGCGGAGACCGGCC
TCCTGAAGGGCGGCGACGTCTATCTGTTTCTGATCGGCATGATGCTGCTCAGTGAAACGGCCCGTGCAAACGGGCTGTTC
GACTGGATCGCGACCTGGGCGGTCAATCATGCCGCAGGTTCAACGGCAAAGCTGTTCACACTGGTCTATCTGGCCGGTGT
CGTAGTGACGATGTTCATGTCCAACGACGCAACGGCTGTTGTGCTGACACCAGCCGTGTTCGAGGCAGCAAAAAAGGCAA
AAGCTGATCCTCTTCCGCTGTTGTTTGCCTGTGCACTCGTTGCCAATGCGGCAAGCTTTGTCCTGCCTGTCTCCAATCCG
GCGAACCTGGTTCTGTATGACGGCGCACTCCCCGCGCTGGGATCATGGATAAGGTCATTCGCGCTCCCGTCGCTCCTGGC
GATCGTGACGACTTATGGTGTTTTGCGCCTCATGGAACAGTCACACTTGCGTCAGGACTGCATCTCCCAGGTTGGGACCA
CCCCATTAACACTAGGCGGCAAAGCGGCTTTTGTCGGCATCGCCCTGACCGCGCTTTTGCTTGTCACAGTGTCCGCTTTT
GATCTGCCTCTCGGCTTACCAACAGCGCTCGCCGGTATTGGAACAGCATTGGGCGTATCGACGCTGGCACGACGATCGCC
GATTGCGCTCATGAGGGATATTTCATGGGGTGTTCTCCCGCTGGTCGGCGGCCTGTTCGTTCTGGTAGCCGCCGTCGAAA
GTGCGGGGGTGGTCGGGGTTCTTGACCACCTGCTTCAACACGCCGCCAATACCGACCTTGTGGCCACAGCTTGGGGAGCT
GGCACTCTTTTGGCCTTCATTTCCAACATCATGAACAACCTGCCTGCTGGATTGATTGCCAGTGAGACAGTGGCGCAGAC
TCATGTATCGCGACTGGTAGTTGATAACCTGTTAATCGGCGTGGATCTTGGCCCAAACCTTTCCGTGACAGGATCTCTGG
CCACAATTCTCTGGTTGCAGGTGATCCGTCGAGAAGGCGAAGAGATCGGGTTTCTGCAATTTCTCAGGGTTGGCGCCGTG
GCGATGCCCGTTGCGCTCCTCACGGTACTCGGTGCCCGGCTTTTGATCGGGTGA

Upstream 100 bases:

>100_bases
ATCCGGTGGCGTTTTATAGTCTGGGCTGTTTTGCAATCTTGTCACTGGGGCTCTGGATAGGCTCGGCATCGATCCTACGC
TCTGCCGATCAGGTGTCGGC

Downstream 100 bases:

>100_bases
ACAGATTAGACGATATTCGCAGGCTGCTGCCAGCCTCAGAGCTGTCCGTAGATAAAATTTATTTTCCGACGAACTCTATG
AATAGATGCGCTTGCTGTCT

Product: arsenical pump membrane protein

Products: ADP; phosphate; arsenite [Periplasm] [C]

Alternate protein names: Arsenic efflux pump protein [H]

Number of amino acids: Translated: 417; Mature: 416

Protein sequence:

>417_residues
MTFHQEAIWAIAALATAGVIIQPFRVPEWVWAVIGAAALVLTGLIPLTMAETGLLKGGDVYLFLIGMMLLSETARANGLF
DWIATWAVNHAAGSTAKLFTLVYLAGVVVTMFMSNDATAVVLTPAVFEAAKKAKADPLPLLFACALVANAASFVLPVSNP
ANLVLYDGALPALGSWIRSFALPSLLAIVTTYGVLRLMEQSHLRQDCISQVGTTPLTLGGKAAFVGIALTALLLVTVSAF
DLPLGLPTALAGIGTALGVSTLARRSPIALMRDISWGVLPLVGGLFVLVAAVESAGVVGVLDHLLQHAANTDLVATAWGA
GTLLAFISNIMNNLPAGLIASETVAQTHVSRLVVDNLLIGVDLGPNLSVTGSLATILWLQVIRREGEEIGFLQFLRVGAV
AMPVALLTVLGARLLIG

Sequences:

>Translated_417_residues
MTFHQEAIWAIAALATAGVIIQPFRVPEWVWAVIGAAALVLTGLIPLTMAETGLLKGGDVYLFLIGMMLLSETARANGLF
DWIATWAVNHAAGSTAKLFTLVYLAGVVVTMFMSNDATAVVLTPAVFEAAKKAKADPLPLLFACALVANAASFVLPVSNP
ANLVLYDGALPALGSWIRSFALPSLLAIVTTYGVLRLMEQSHLRQDCISQVGTTPLTLGGKAAFVGIALTALLLVTVSAF
DLPLGLPTALAGIGTALGVSTLARRSPIALMRDISWGVLPLVGGLFVLVAAVESAGVVGVLDHLLQHAANTDLVATAWGA
GTLLAFISNIMNNLPAGLIASETVAQTHVSRLVVDNLLIGVDLGPNLSVTGSLATILWLQVIRREGEEIGFLQFLRVGAV
AMPVALLTVLGARLLIG
>Mature_416_residues
TFHQEAIWAIAALATAGVIIQPFRVPEWVWAVIGAAALVLTGLIPLTMAETGLLKGGDVYLFLIGMMLLSETARANGLFD
WIATWAVNHAAGSTAKLFTLVYLAGVVVTMFMSNDATAVVLTPAVFEAAKKAKADPLPLLFACALVANAASFVLPVSNPA
NLVLYDGALPALGSWIRSFALPSLLAIVTTYGVLRLMEQSHLRQDCISQVGTTPLTLGGKAAFVGIALTALLLVTVSAFD
LPLGLPTALAGIGTALGVSTLARRSPIALMRDISWGVLPLVGGLFVLVAAVESAGVVGVLDHLLQHAANTDLVATAWGAG
TLLAFISNIMNNLPAGLIASETVAQTHVSRLVVDNLLIGVDLGPNLSVTGSLATILWLQVIRREGEEIGFLQFLRVGAVA
MPVALLTVLGARLLIG

Specific function: Involved in arsenical resistance. Thought to form the channel of an arsenite pump [H]

COG id: COG1055

COG function: function code P; Na+/H+ antiporter NhaD and related arsenite permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ArsB family [H]

Homologues:

Organism=Escherichia coli, GI87082277, Length=364, Percent_Identity=29.3956043956044, Blast_Score=110, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000802 [H]

Pfam domain/function: PF02040 ArsB [H]

EC number: NA

Molecular weight: Translated: 43376; Mature: 43245

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFHQEAIWAIAALATAGVIIQPFRVPEWVWAVIGAAALVLTGLIPLTMAETGLLKGGDV
CCCHHHHHHHHHHHHHCCHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
YLFLIGMMLLSETARANGLFDWIATWAVNHAAGSTAKLFTLVYLAGVVVTMFMSNDATAV
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEE
VLTPAVFEAAKKAKADPLPLLFACALVANAASFVLPVSNPANLVLYDGALPALGSWIRSF
EECHHHHHHHHHCCCCCCHHHHHHHHHHCHHHEEEECCCCCCEEEECCCHHHHHHHHHHH
ALPSLLAIVTTYGVLRLMEQSHLRQDCISQVGTTPLTLGGKAAFVGIALTALLLVTVSAF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHHHHHHH
DLPLGLPTALAGIGTALGVSTLARRSPIALMRDISWGVLPLVGGLFVLVAAVESAGVVGV
CCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHH
LDHLLQHAANTDLVATAWGAGTLLAFISNIMNNLPAGLIASETVAQTHVSRLVVDNLLIG
HHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHEE
VDLGPNLSVTGSLATILWLQVIRREGEEIGFLQFLRVGAVAMPVALLTVLGARLLIG
EECCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TFHQEAIWAIAALATAGVIIQPFRVPEWVWAVIGAAALVLTGLIPLTMAETGLLKGGDV
CCHHHHHHHHHHHHHCCHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
YLFLIGMMLLSETARANGLFDWIATWAVNHAAGSTAKLFTLVYLAGVVVTMFMSNDATAV
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEE
VLTPAVFEAAKKAKADPLPLLFACALVANAASFVLPVSNPANLVLYDGALPALGSWIRSF
EECHHHHHHHHHCCCCCCHHHHHHHHHHCHHHEEEECCCCCCEEEECCCHHHHHHHHHHH
ALPSLLAIVTTYGVLRLMEQSHLRQDCISQVGTTPLTLGGKAAFVGIALTALLLVTVSAF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHHHHHHH
DLPLGLPTALAGIGTALGVSTLARRSPIALMRDISWGVLPLVGGLFVLVAAVESAGVVGV
CCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHH
LDHLLQHAANTDLVATAWGAGTLLAFISNIMNNLPAGLIASETVAQTHVSRLVVDNLLIG
HHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHEE
VDLGPNLSVTGSLATILWLQVIRREGEEIGFLQFLRVGAVAMPVALLTVLGARLLIG
EECCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; arsenite [Cytoplasm]; H2O [C]

Specific reaction: ATP + arsenite [Cytoplasm] + H2O = ADP + phosphate + arsenite [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12950922 [H]